RESUMO
The establishment of neural circuits depends on the ability of axonal growth cones to sense their surrounding environment en route to their target. To achieve this, a coordinated rearrangement of cytoskeleton in response to extracellular cues is essential. Although previous studies have identified different chemotropic and adhesion molecules that influence axonal development, the molecular mechanism by which these signals control the cytoskeleton remains poorly understood. Here, we show that in vivo conditional ablation of the focal adhesion kinase gene (Fak) from mouse hippocampal pyramidal cells impairs axon outgrowth and growth cone morphology during development, which leads to functional defects in neuronal connectivity. Time-lapse recordings and in vitro FRAP analysis indicate that filopodia motility is altered in growth cones lacking FAK, probably owing to deficient actin turnover. We reveal the intracellular pathway that underlies this process and describe how phosphorylation of the actin nucleation-promoting factor N-WASP is required for FAK-dependent filopodia formation. Our study reveals a novel mechanism through which FAK controls filopodia formation and actin nucleation during axonal development.
Assuntos
Actinas/metabolismo , Axônios/fisiologia , Proteína-Tirosina Quinases de Adesão Focal/metabolismo , Rede Nervosa/crescimento & desenvolvimento , Neurônios/citologia , Pseudópodes/fisiologia , Animais , Axônios/enzimologia , Primers do DNA/genética , Eletroporação , Potenciais Pós-Sinápticos Excitadores/fisiologia , Recuperação de Fluorescência Após Fotodegradação , Proteína-Tirosina Quinases de Adesão Focal/genética , Cones de Crescimento/fisiologia , Imuno-Histoquímica , Imunoprecipitação , Camundongos , Camundongos Transgênicos , Faloidina , Pseudópodes/enzimologiaRESUMO
Axon refinement is a necessary event for sculpting the final wiring of neural circuits. Although some factors have been identified that cause axonal arbor remodeling, the molecular pathways transducing these extracellular signals to adhesion disassembly and the cytoskeleton are poorly understood. Here we show that conditional ablation of Focal adhesion kinase (Fak) abolishes axon remodeling induced by Semaphorin-3A (Sema3A) in hippocampal neurons. Sema3A elicits divergent effects on different tyrosine residues of FAK: it increases phosphorylation of Tyr397, the kinase domain and the tyrosine residue 925, and decreases phosphorylation of Tyr407 and Tyr861. Moreover, Sema3A mediates mechanisms that contribute to the disassembly of adhesion contacts in a FAK-dependent manner: tyrosine phosphorylation of alpha-actinin and FAKY925 that decreases FAK-Paxillin interaction. Altogether, our results provide novel insights into the spatiotemporal dynamics of FAK activation mediated by Sema3A and on its interaction with its downstream effectors: Paxillin and alpha-actinin in neurons.
Assuntos
Axônios/metabolismo , Encéfalo/crescimento & desenvolvimento , Encéfalo/metabolismo , Proteína-Tirosina Quinases de Adesão Focal/metabolismo , Semaforina-3A/metabolismo , Actinina/metabolismo , Animais , Encéfalo/citologia , Adesão Celular/fisiologia , Células Cultivadas , Ativação Enzimática/fisiologia , Inibidores Enzimáticos/farmacologia , Proteína-Tirosina Quinases de Adesão Focal/química , Proteína-Tirosina Quinases de Adesão Focal/genética , Adesões Focais/genética , Adesões Focais/metabolismo , Adesões Focais/ultraestrutura , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Paxilina/metabolismo , Fosforilação , Estrutura Terciária de Proteína/fisiologia , Semaforina-3A/genética , Tirosina/metabolismoRESUMO
Pairing of homologous chromosomes is a crucial step in meiosis, which in fission yeast depends on nuclear oscillations. However, how nuclear oscillations help pairing is unknown. Here, we show that homologous loci typically pair when the spindle pole body is at the cell pole and the nucleus is elongated, whereas they unpair when the spindle pole body is in the cell center and the nucleus is round. Inhibition of oscillations demonstrated that movement is required for initial pairing and that prolonged association of loci leads to mis-segregation. The double-strand break marker Rec25 accumulates in elongated nuclei, indicating that prolonged chromosome stretching triggers recombinatory pathways leading to mis-segregation. Mis-segregation is rescued by overexpression of the Holliday junction resolvase Mus81, suggesting that prolonged pairing results in irresolvable recombination intermediates. We conclude that nuclear oscillations exhibit a dual role, promoting initial pairing and restricting the time of chromosome associations to ensure proper segregation.
Assuntos
Núcleo Celular/metabolismo , Cromossomos Fúngicos/metabolismo , Meiose , Schizosaccharomyces/citologia , Schizosaccharomyces/metabolismo , Pareamento Cromossômico , Segregação de Cromossomos , Loci Gênicos , Proteínas de Fluorescência Verde/metabolismo , Resolvases de Junção Holliday , Schizosaccharomyces/genética , Proteínas de Schizosaccharomyces pombe/metabolismo , Corpos Polares do Fuso/metabolismo , Imagem com Lapso de TempoRESUMO
During cell division, spindle microtubules attach to chromosomes through kinetochores, protein complexes on the chromosome. The central question is how microtubules find kinetochores. According to the pioneering idea termed search-and-capture, numerous microtubules grow from a centrosome in all directions and by chance capture kinetochores. The efficiency of search-and-capture can be improved by a bias in microtubule growth towards the kinetochores, by nucleation of microtubules at the kinetochores and at spindle microtubules, by kinetochore movement, or by a combination of these processes. Here we show in fission yeast that kinetochores are captured by microtubules pivoting around the spindle pole, instead of growing towards the kinetochores. This pivoting motion of microtubules is random and independent of ATP-driven motor activity. By introducing a theoretical model, we show that the measured random movement of microtubules and kinetochores is sufficient to explain the process of kinetochore capture. Our theory predicts that the speed of capture depends mainly on how fast microtubules pivot, which was confirmed experimentally by speeding up and slowing down microtubule pivoting. Thus, pivoting motion allows microtubules to explore space laterally, as they search for targets such as kinetochores.
Assuntos
Cinetocoros/metabolismo , Microtúbulos/metabolismo , Schizosaccharomyces/fisiologia , Fuso Acromático/metabolismo , Trifosfato de Adenosina/fisiologia , Adenilil Imidodifosfato/farmacologia , Cromossomos Fúngicos/metabolismo , Recuperação de Fluorescência Após Fotodegradação , Proteínas de Fluorescência Verde/metabolismo , Cinética , Microscopia de Fluorescência , Proteínas Associadas aos Microtúbulos/metabolismo , Mitose , Modelos Biológicos , Proteínas Recombinantes de Fusão/metabolismo , Schizosaccharomyces/ultraestrutura , Proteínas de Schizosaccharomyces pombe/metabolismo , Imagem com Lapso de TempoRESUMO
During the formation of neural circuitry, axons are known to be guided to their specific targets by a relatively small arsenal of guidance signals. However, the molecular integration of this guidance information inside the axonal growth cone (GC) is still baffling. Focal adhesion kinase (FAK) is a cytosolic kinase which interacts with a complex molecular network via multiple phosphorylation sites. Paradoxically, FAK activation is required by both attractive and repulsive cues to control respectively axon outgrowth and disassembly of adhesive structures together with cytoskeletal dynamics. It was suggested that FAK might work as a versatile molecular integrator switching to different functions depending on its activation state. Two studies published recently by our group and Woo et al. shed light on this issue: for the first time, these works report a detailed molecular analysis of FAK activation and phosphorylation pattern in primary neuronal cultures in response to the repulsive cues Semaphorin3A and ephrinA1 respectively. Here we comment on the major novelties provided by these papers in the context of previous literature and we speculate on the future avenues of investigation opened by these works.
Assuntos
Efrina-A1/metabolismo , Proteína-Tirosina Quinases de Adesão Focal/metabolismo , Cones de Crescimento/fisiologia , Semaforina-3A/metabolismo , Adesão Celular/fisiologia , Moléculas de Adesão Celular/metabolismo , Moléculas de Adesão Celular/fisiologia , Movimento Celular/fisiologia , Células Cultivadas , Efrina-A1/fisiologia , Cones de Crescimento/metabolismo , Células HEK293 , Humanos , Fatores de Crescimento Neural/metabolismo , Fatores de Crescimento Neural/fisiologia , Netrina-1 , Neurônios/metabolismo , Neurônios/fisiologia , Fosforilação , Semaforina-3A/fisiologia , Transdução de Sinais/fisiologia , Proteínas Supressoras de Tumor/metabolismo , Proteínas Supressoras de Tumor/fisiologiaRESUMO
The capacity of the fungus Trichoderma harzianum CECT 2413 to colonize roots and stimulate plant growth was analyzed. Tobacco seedlings (Nicotiana benthamiana) transferred to Petri dishes inoculated with T. harzianum conidia showed increased plant fresh weight (140%) and foliar area (300%), as well as the proliferation of secondary roots (300%) and true leaves (140%). The interaction between strain CECT 2413 and the tomato-root system was also studied during the early stages of root colonization by the fungus. When T. harzianum conidia were inoculated into the liquid medium of hydroponically grown tomato plants (Lycopersicum esculentum), profuse adhesion of hyphae to the plant roots as well as colonization of the root epidermis and cortex were observed. Confocal microscopy of a T. harzianum transformant that expressed the green fluorescent protein (GFP) revealed intercellular hyphal growth and the formation of plant-induced papilla-like hyphal tips. Analysis of the T. harzianum-tomato interaction in soil indicated that the contact between T. harzianum and the roots persisted over a long period of time. This interaction was characterized by the presence of yeast-like cells, a novel and previously undescribed developmental change. To study the molecular mechanism underlying fungal ability to colonize the tomato-root system, the T. harzianum transcriptome was analyzed during the early stages of the plant-fungus interaction. The expression of fungal genes related to redox reactions, lipid metabolism, detoxification, and sugar or amino-acid transport increased when T. harzianum colonized tomato roots. These observations are similar to those regarding the interactions of mycorrhiza and pathogenic fungi with plants.