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BACKGROUND: Low temperature is a crucial stress factor of wheat (Triticum aestivum L.) and adversely impacts on plant growth and grain yield. Multi-million tons of grain production are lost annually because crops lack the resistance to survive in winter. Particularlly, winter wheat yields was severely damaged under extreme cold conditions. However, studies about the transcriptional and metabolic mechanisms underlying cold stresses in wheat are limited so far. RESULTS: In this study, 14,466 differentially expressed genes (DEGs) were obtained between wild-type and cold-sensitive mutants, of which 5278 DEGs were acquired after cold treatment. 88 differential accumulated metabolites (DAMs) were detected, including P-coumaroyl putrescine of alkaloids, D-proline betaine of mino acids and derivativ, Chlorogenic acid of the Phenolic acids. The comprehensive analysis of metabolomics and transcriptome showed that the cold resistance of wheat was closely related to 13 metabolites and 14 key enzymes in the flavonol biosynthesis pathway. The 7 enhanced energy metabolites and 8 up-regulation key enzymes were also compactly involved in the sucrose and amino acid biosynthesis pathway. Moreover, quantitative real-time PCR (qRT-PCR) revealed that twelve key genes were differentially expressed under cold, indicating that candidate genes POD, Tacr7, UGTs, and GSTU6 which were related to cold resistance of wheat. CONCLUSIONS: In this study, we obtained the differentially expressed genes and differential accumulated metabolites in wheat under cold stress. Using the DEGs and DAMs, we plotted regulatory pathway maps of the flavonol biosynthesis pathway, sucrose and amino acid biosynthesis pathway related to cold resistance of wheat. It was found that candidate genes POD, Tacr7, UGTs and GSTU6 are related to cold resistance of wheat. This study provided valuable molecular information and new genetic engineering clues for the further study on plant resistance to cold stress.
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Regulação da Expressão Gênica de Plantas , Triticum , Aminoácidos/metabolismo , Grão Comestível/metabolismo , Flavonóis/metabolismo , Sacarose/metabolismo , Triticum/genética , Triticum/metabolismoRESUMO
The spikelet number and heading date are two crucial and correlated traits for yield in wheat. Here, a quantitative trait locus (QTL) analysis was conducted in F8 recombinant inbred lines (RILs) derived from crossing two common wheats with different spikelet numbers. A total of 15 stable QTL influencing total spikelet number (TSN) and heading date (HD) were detected. Notably, FT-D1, a well-known flowering time gene in wheat, was located within the finely mapped interval of a major QTL on 7DS (QTsn/Hd.cau-7D). A causal indel of one G in the third exon of FT-D1 was significantly associated with total spikelet number and heading date. Consistently, CRISPR/Cas9 mutant lines with homozygous mutations in FT-D1 displayed an increase in total spikelet number and heading date when compared with wild type. Moreover, one simple and robust marker developed according to the polymorphic site of FT-D1 revealed that this one G indel had been preferentially selected to adapt to different environments. Collectively, these data provide further insights into the genetic basis of spikelet number and heading date, and the diagnostic marker of FT-D1 will be useful for marker-assisted pyramiding in wheat breeding.
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Melhoramento Vegetal , Triticum , Éxons/genética , Nucleotídeos , Locos de Características Quantitativas/genética , Triticum/genéticaRESUMO
KEY MESSAGE: QHd.cau-7D.1 for heading date was delimited into the physical interval of approximately 17.38 Mb harboring three CONSTANS-like zinc finger genes. Spike morphological traits, plant height and heading date play important roles in yield improvement of wheat. To reveal the genetic factors that controlling spike morphological traits, plant height and heading date on the D genome, we conducted analysis of quantitative traits locus (QTL) using 198 F7:8 recombinant inbred lines (RILs) derived from a cross between the common wheat TAA10 and resynthesized allohexaploid wheat XX329 with similar AABB genomes. A total of 23 environmentally stable QTL on the D sub-genome for spike length (SL), fertile spikelet number per spike (FSN), sterile spikelet number per spike (SSN), total spikelet number per spike (TSN), spike compactness (SC), plant height (PHT) and heading date (HD) were detected, among which eight appeared to be novel QTL. Furthermore, QHd.cau-7D.1 and QPht.cau-7D.2 shared identical confidence interval and were delimited into the physical interval of approximately 17.38 Mb with 145 annotated genes, including three CONSTANS-like zinc finger genes (TraesCS7D02G209000, TraesCS7D02G213000 and TraesCS7D02G220300). This study will help elucidate the molecular mechanism of the seven traits (SL, FSN, SSN, TSN, SC, PHT and HD) and provide a potentially valuable resource for genetic improvement.
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Locos de Características Quantitativas , Triticum , Ligação Genética , Fenótipo , Triticum/genéticaRESUMO
Rhizoctonia cerealis is the causal agent of sharp eyespot, a devastating disease of cereal crops including wheat. Several metalloproteases have been implicated in pathogenic virulence, but little is known about whole-genome metalloproteases in R. cerealis. In this study, a total of 116 metalloproteases-encoding genes were identified and characterized from the R. cerealis Rc207 genome. The gene expression profiles and phylogenetic relationship of 11 MEP36/fungalysin metalloproteases were examined during the fungal infection to wheat, and function of an upregulated secretory MEP36 named RcFL1 was validated. Of 11 MEP36 family metalloproteases, ten, except RcFL5, were predicted to be secreted proteins and nine encoding genes, but not RcFL5 and RcFL2, were expressed during the R. cerealis infection process. Phylogenetic analysis suggested that MEP36 metalloproteases in R. cerealis were closely related to those of Rhizoctonia solani but were remote to those of Bipolaris sorokiniana, Fusarium graminearum, F. pseudograminearum, and Pyricularia oryzae. Expression of RcFL1 was significantly upregulated during the infection process and induced plant cell death in wheat to promote the virulence of the pathogen. The MEP36 domain was necessary for the activities of RcFL1. Furthermore, RcFL1 could repress the expression of wheat genes coding for the chitin elicitor receptor kinase TaCERK1 and chitinases. These results suggest that this MEP36 metalloprotease RcFL1 may function as a virulence factor of R. cerealis through inhibiting host chitin-triggered immunity and chitinases. This study provides insights on pathogenic mechanisms of R. cerealis. RcFL1 likely is an important gene resource for improving resistance of wheat to R. cerealis through host-induced gene silencing strategy.
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Quitinases , Triticum , Basidiomycota , Quitina/metabolismo , Quitinases/metabolismo , Metaloproteases/genética , Metaloproteases/metabolismo , Filogenia , Doenças das Plantas/microbiologia , Rhizoctonia/fisiologia , Triticum/metabolismo , Virulência/genética , Fatores de Virulência/genética , Fatores de Virulência/metabolismoRESUMO
KEY MESSAGE: A novel wax locus GLOSSY1 was finely mapped to an approximately 308.1-kbp genomic interval on chromosome 2DS of wheat. The epicuticular wax, the outermost layer of aerial organs, gives plants their bluish-white (glaucous) appearance. Epicuticular wax is ubiquitous and provides an essential protective function against environmental stresses. In this study, we identified the glossy1 mutant on the basis of its glossy glume from an EMS population in the elite wheat (Triticum aestivum L.) cultivar Jimai22. The mutant had a dramatically different profile in total wax load and composition of individual wax constituents relative to the wild type, resulting in the increased cuticle permeability of glumes. The glossy glume phenotype was controlled by a single, semidominant locus mapping to the short arm of chromosome 2D, within a 308.1-kbp genomic interval that contained ten annotated protein-coding genes. These results pave the way for an in-depth analysis of the underlying genetic basis of wax formation patterns and enrich our understanding of mechanisms regulating wax metabolism.
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Oxirredutases do Álcool/genética , Mapeamento Cromossômico/métodos , Cromossomos de Plantas/genética , Regulação da Expressão Gênica de Plantas , Mutação , Proteínas de Plantas/genética , Triticum/genética , Ligação Genética , Marcadores Genéticos , FenótipoRESUMO
KEY MESSAGE: GWAS identified stable loci for TGW and stress tolerance in winter wheat based on two sowing conditions, which will provide opportunities for developing new cultivars with high yield and yield stability. Wheat is an important food crop widely cultivated in the world. Breeding new varieties with high yields and superior adaptability is the main goal of modern wheat breeding program. In order to determine the marker-trait associations (MATs), a set of 688 diverse winter wheat accessions were subjected to genome-wide association study (GWAS) using the wheat 90K array. Field trials under normal-sown (NS) and late-sown (LS) conditions were conducted for thousand grain weight (TGW) and stress susceptibility index (SSI) at three different sites across two consecutive years. A total of 179 (NS) and 158 (LS) MATs corresponded with TGW; of these, 16 and 6 stable MATs for TGWNS and TGWLS were identified on chromosomes 1B, 2B, 3A, 3B, 5A, 5B, 5D, 6B, and 7D across at least three environments. Notably, a QTL hot spot controlling TGW under NS and LS conditions was found on chromosome 5A (140-142 cM). Moreover, 8 of 228 stable MATs on chromosomes 4B, 5A, and 5D for SSI were detected. A haplotype block associated with TGW and SSI was located on chromosome 5A at 91 cM, nearby the vernalization gene VRN-A1. Additionally, analysis of wheat varieties from the different eras revealed that the grain weight and stress tolerance are not improved concurrently. Overall, our results provide promising alleles controlling grain weight and stress tolerance (particularly for thermotolerance) for wheat breeders, which can be used in marker-assisted selection for improving grain yield and yield stability in wheat.
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Locos de Características Quantitativas , Sementes/crescimento & desenvolvimento , Triticum/genética , Grão Comestível/genética , Meio Ambiente , Estudos de Associação Genética , Genética Populacional , Haplótipos , Desequilíbrio de Ligação , Polimorfismo de Nucleotídeo Único , Estresse FisiológicoRESUMO
The development of a biodegradable cellulose-based separator with excellent performance has been of great research significance and application potential for the green development of supercapacitors. Herein, the regenerated porous cellulose/Polyvinyl alcohol films (CP-10, CP-15, CP-20, CP-25) with different mass ratio were successfully fabricated by a simple blending and phase inversion process. Their electrochemical properties as separators in assembled supercapacitor were evaluated. Fourier transform infrared spectroscopy and x-ray diffraction analysis indicate that intermolecular and intramolecular hydrogen bonding existed between cellulose and polyvinyl alcohol of the CP films. Compared with other CP films, the CP-20 film shows higher mechanical strength (28.02 MPa), better wettability (79.06°), higher porosity (59.69%) and electrolyte uptake (281.26 wt%). These properties of CP-20 are expected to show better electrochemical performance as separator. Indeed, the electrochemical tests, including electrochemical impedance spectroscopy, cyclic voltammetry, galvanostatic charge discharge, demonstrate that the SC-20 capacitor (with CP-20 as separator) shows the lowest equivalent series resistance of 0.57 Ω, the highest areal capacitance of 1.98 F cm-2 at 10 mV s-1, specific capacitance of 134.41 F g-1 and charge-discharge efficiency of 98.62% at 1 A g-1 among the four capacitors with CP films as separators. Comparing the assembled SC-40 and SC-30 with two commercial separators (TF4040 and MPF30AC) and SC-PVA with Polyvinyl alcohol (PVA) separator, the CV and GCD curves of SC-20 maintain the quasi rectangular and symmetrical triangular profiles respectively at different scan rates in potential window of 0-1 V. SC-20 exhibits the highest value of 28.24 Wh kg-1 at 0.5 A g-1 with a power density of 0.26 kW kg-1, and 13.41 Wh kg-1 at 10 A g-1 with a power density of 6.04 kW kg-1. SC-20 also shows the lowest voltage drop and the highest areal and specific capacitance. Moreover, SC-20 maintains the highest value of 86.81% after 4000 cycles compared to 21.18% of SC-40, 75.07% of SC-30, and 6.66% of SC-PVA, showing a superior rate capability of a supercapacitor. These results indicate that CP films can be served as promising separators for supercapacitors.
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KEY MESSAGE: This study dissected and validated a QTL cluster associated with thousand grain weight on chromosome 4B using multiple near-isogenic lines in common wheat. Grain size and weight are crucial components of wheat yield. Previously, we identified a QTL cluster for thousand grain weight (TGW) on chromosome 4B using the Nongda3338 (ND3338)/Jingdong6 (JD6) doubled haploid population. Here, near-isogenic lines (NILs) in the ND3338 background were developed to dissect and validate the QTL cluster. Based on six independent BC3F3:4 heterogeneous inbred families, the 4B QTL cluster was divided into two linked QTL intervals (designated 4B.1 and 4B.2 QTL). For the 4B.1 QTL, the Rht-B1 gene, of which Rht-B1b allele reduces plant height (PH) by 21.18-29.34 cm (34.34-53.71%), was demonstrated to be the most likely candidate gene with pleiotropic effects on grain size and TGW. For the 4B.2 QTL, the NILJD6 consistently showed an increase in TGW of 3.51-7.68 g (8.84-22.77%) compared with NILND3338 across different field trials, along with a significant increase in PH of 2.26-6.71 cm (3.92-12.01%). Moreover, both QTL intervals had a larger effect on grain width than on grain length. Additionally, the first significant difference in 100-grain fresh weight and 100-grain dry weight between the NIL pairs of the 4B.1 QTL interval (Rht-B1) was observed at 6 days after pollination (DAP), while the differences were first visible at 30 DAP for the 4B.2 QTL interval. Collectively, our work provides a new example of QTL dissection for grain weight in wheat and lays a foundation for further map-based cloning of the major QTL that have potential applications in wheat molecular breeding for high yield.
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Locos de Características Quantitativas , Sementes/crescimento & desenvolvimento , Triticum/genética , Mapeamento Cromossômico , Ligação Genética , Pleiotropia Genética , Haploidia , Repetições de Microssatélites , Fenótipo , Melhoramento Vegetal , Polimorfismo de Nucleotídeo Único , Sementes/genéticaRESUMO
Lithium-sulfur (Li-S) batteries as a new generation of high energy batteries, with low cost and environmentally friendly, have a broad application prospects. While the poor conductivity of sulfur, the volume effect and 'shuttle effect' during charge and discharge, and slow redox kinetics of polysulfide intermediates still limit the practical application. To solve these problems, we synthesize a valid 3D hybrid material (Co9S8@N-CHS) of nanosized Co9S8 evenly distributed on N-doped carbon hollow spheres with strong chemical coupling by in situ carbonization of Co(NO3)2@resorcinol/formaldehyde and sulfidation. It presents a high electronic conductivity, absorbing chemical adsorption capability to polysulfides and can catalyze the sulfur redox processes. Compared with S/AC and S/N-CHS electrodes, S/Co9S8@N-CHS electrodes achieve an excellent initial discharge specific capacity of 1337 mAh g-1 at 0.1 C and a long cycle life with an ultralow capacity decay of 0.027% per cycle over 1000 cycles at 1.0 C and the coulombic efficiency is above 99%. Consequently, it is an effective sulfur host material for high performance Li-S batteries.
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BACKGROUND: High-molecular-weight glutenin subunits (HMW-GS) play important roles in the elasticity of dough made from wheat. The HMW-GS null line is useful for studying the contribution of HMW-GS to the end-use quality of wheat. METHODS: In a previous work, we cloned the Glu-1Ebx gene from Thinopyrum bessarabicum and introduced it into the wheat cultivar, Bobwhite. In addition to lines expressing the Glu-1Ebx gene, we also obtained a transgenic line (LH-11) with all the HMW-GS genes silenced. The HMW-GS deletion was stably inherited as a dominant and conformed to Mendel's laws. Expression levels of HMW-GS were determined by RT-PCR and epigenetic changes in methylation patterns and small RNAs were analyzed. Glutenins and gliadins were separated and quantitated by reversed-phase ultra-performance liquid chromatography. Measurement of glutenin macropolymer, and analysis of agronomic traits and end-use quality were also performed. RESULTS: DNA methylation and the presence of small double-stranded RNA may be the causes of post-transcriptional gene silencing in LH-11. The accumulation rate and final content of glutenin macropolymer (GMP) in LH-11 were significantly lower than in wild-type (WT) Bobwhite. The total protein content was not significantly affected as the total gliadin content increased in LH-11 compared to WT. Deletion of HMW-GS also changed the content of different gliadin fractions. The ratio of ω-gliadin increased, whereas α/ß- and γ-gliadins declined in LH-11. The wet gluten content, sedimentation value, development time and stability time of LH-11 were remarkably lower than that of Bobwhite. Bread cannot be made using the flour of LH-11. CONCLUSIONS: Post-transcriptional gene silencing through epigenetic changes and RNA inhibition appear to be the causes for the gene expression deficiency in the transgenic line LH-11. The silencing of HMW-GW in LH-11 significantly reduced the dough properties, GMP content, wet gluten content, sedimentation value, development time and stability time of flour made from this wheat cultivar. The HMW-GS null line may provide a potential material for biscuit-making because of its low dough strength.
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Pão , Glutens/metabolismo , Proteínas de Plantas/metabolismo , Triticum/metabolismo , Pão/normas , Culinária , Metilação de DNA , Farinha , Glutens/genética , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , Interferência de RNA , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Triticum/genéticaRESUMO
Bi-based YbMg2Bi1.98 Zintl compounds represent promising thermoelectric materials. Precise composition and appropriate doping are of great importance for this complex semiconductor. Here, the influence of Zn substitution for Mg on the microstructure and thermoelectric properties of p-type YbMg1.85-xZnxBi1.98 (x = 0, 0.05, 0.08, 0.13, 0.23) was investigated. Polycrystalline samples were prepared using induction melting and densified with spark plasma sintering. X-ray diffraction confirmed that the major phase of the samples possesses the trigonal CaAl2Si2-type crystal structure, and SEM/EDS indicated the presence of minor secondary phases. The electrical conductivity increases and the lattice thermal conductivity decreases with more Zn doping in YbMg1.85-xZnxBi1.98, whereas the Seebeck coefficient has a large reduction. The band gap decreases with increasing Zn concentration and leads to bipolar conduction, resulting in an increase in the thermal conductivity at higher temperatures. Figure of merit ZT values of 0.51 and 0.49 were found for the samples with x = 0 and 0.05 at 773 K, respectively. The maximum amount of Zn doping is suggested to be less than x = 0.1.
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Double-layered manganites are natural superlattices with low thermal conductivity, which is of importance for potential thermoelectric applications. The Gd2-2xSr1+2xMn2O7 (x = 0.5; 0.625; 0.75) were prepared by the solid-state reaction method. All the samples crystallize in the tetragonal I4/mmm Sr3Ti2O7 type structure. The unit cell volume and the distortion in the MnO6 octahedra increase with increasing Gd content. Their thermoelectric properties were investigated between 300 and 1200 K. All exhibit an n-type semiconducting behavior. The electrical conductivity (σ) increases while the absolute value of the Seebeck coefficient (|S|) decreases with increasing Gd content. Simultaneous increases in σ and |S| with increasing temperature are observed at temperatures approximately higher than 600 K, and the power factor reaches a maximum value of 18.36 µW/(m K²) for x = 0.75 at 1200 K. The thermal conductivity (κ) is lower than 2 W/(m K) over the temperature range of 300-1000 K for all the samples and a maximum dimensionless figure of merit ZT of 0.01 is obtained for x = 0.75 at 1000 K.
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Sitophilus zeamais (maize weevil) is one of the most destructive pests that seriously affects the quantity and quality of wheat (Triticum aestivum L.). However, little is known about the constitutive defense mechanism of wheat kernels against maize weevils. In this study, we obtained a highly resistant variety RIL-116 and a highly susceptible variety after two years of screening. The morphological observations and germination rates of wheat kernels after feeding ad libitum showed that the degree of infection in RIL-116 was far less than that in RIL-72. The combined analysis of metabolome and transcriptome of RIL-116 and RIL-72 wheat kernels revealed differentially accumulated metabolites were mainly enriched in flavonoids biosynthesis-related pathway, followed by glyoxylate and dicarboxylate metabolism, and benzoxazinoid biosynthesis. Several flavonoids metabolites were significantly up-accumulated in resistant variety RIL-116. In addition, the expression of structural genes and transcription factors (TFs) related to flavonoids biosynthesis were up-regulated to varying degrees in RIL-116 than RIL-72. Taken together, these results indicated that the biosynthesis and accumulation of flavonoids contributes the most to wheat kernels defense against maize weevils. This study not only provides insights into the constitutive defense mechanism of wheat kernels against maize weevils, but may also play an important role in the breeding of resistant varieties.
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Antiferroelectrics (AFEs) are promising candidates in energy-storage capacitors, electrocaloric solid-cooling, and displacement transducers. As an actively studied lead-free antiferroelectric (AFE) material, NaNbO3 has long suffered from its ferroelectric (FE)-like polarization-electric field (P-E) hysteresis loops with high remnant polarization and large hysteresis. Guided by theoretical calculations, a new strategy of reducing the oxygen octahedral tilting angle is proposed to stabilize the AFE P phase (Space group Pbma) of NaNbO3. To validate this, we judiciously introduced CaHfO3 with a low Goldschmidt tolerance factor and AgNbO3 with a low electronegativity difference into NaNbO3, the decreased cation displacements and [BO6] octahedral tilting angles were confirmed by Synchrotron X-ray powder diffraction and aberration-corrected scanning transmission electron microscopy. Of particular importance is that the 0.75NaNbO3-0.20AgNbO3-0.05CaHfO3 ceramic exhibits highly reversible phase transition between the AFE and FE states, showing well-defined double P-E loops and sprout-shaped strain-electric field curves with reduced hysteresis, low remnant polarization, high AFE-FE phase transition field, and zero negative strain. Our work provides a new strategy for designing NaNbO3-based AFE material with well-defined double P-E loops, which can also be extended to discover a variety of new lead-free AFEs.
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Wheat as a staple food crop is enduring ever-frequent intermittent and changing drought with the climate change. It is of great significance to highlight the adaptive approaches under such variable conditions at multiple levels to provide a comprehensive understanding of drought tolerance and facilitate the genetic breeding of wheat. Therefore, three wheat lines with different drought tolerance (drought-tolerant mutant Mu > common wheat CK > drought susceptible mutant mu) were analyzed under moderate and severe drought stresses as well as rehydration. Samples were subjected to transcriptomic and metabolomic profiling in combination with physiological and biochemical determination. The moderate drought stress rendered 198 and 115 differentially expressed metabolites (DEMs) in CK and Mu, respectively. The severe drought stress rendered 166, 151 and 137 DEMs in CK, Mu and mu, respectively. The rehydration rendered 150 and 127 DEMs in CK and Mu. 12,557 and 10,402 differentially expressed genes (DEGs) were identified for CK and Mu under moderate drought stress, respectively. 9,893, 7,924, and 9,387 DEGs were identified for CK, Mu, and mu under severe drought stress, respectively. 13,874 and 14,839 were identified in CK and Mu under rehydration, respectively. Metabolomics results showed that amino acid was the most differentially expressed metabolites, followed by phenolic acids. Flavonoids played an important role in drought tolerance. Most enriched pathways under drought included biosynthesis of secondary metabolites, metabolic pathways and photosynthesis. Metabolites and genes involved in osmotic regulation, antioxidase activities, and ABA signaling were more enriched in Mu than in CK and mu. Various drought-responsive genes and metabolites in Mu showed different trends with those in CK and mu. Increased amino acids biosynthetic capability and ROS scavenging ability resulted from higher antioxidase activities and increased flavonoids may be the mechanisms underlying the drought tolerance characteristic of Mu. Recovery from reversible ROS damage and rapid amino acid biosynthesis may contribute to the rapid recovery of Mu. The present study provides new insights for mechanisms of wheat under complex drought conditions.
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The lead-bismuth eutectic (LBE) can be easily activated by neutron radiation to produce the radionuclide 210Po. It is therefore necessary to establish an effective method to remove vaporized polonium in the cover gas to prevent its release into the air in scenarios of reactor maintenance and coolant leakage accidents. This paper presents a SiO2 nanofiber membrane prepared based on the electrostatic spinning and calcination process. The SiO2 nanofiber membrane had the advantages of good flexibility, high-temperature resistance, and corrosion resistance. In the trapping experiments, the SiO2 nanofiber membrane filters showed excellent filtration performance at 300~400 °C, and the filtration efficiencies for Te, Pb, and Bi could reach 99%, 99%, and 98%, respectively. Proper filtration temperature and gas flow rate are important to maintain high filtration efficiency. After five cycles, the SiO2 nanofiber membrane filter still exhibited excellent cycle-use performance. In the density functional theory (DFT) calculations, PbPo and PbTe had strong interactions with amorphous SiO2, having adhesion energies of -2.96 to -2.83 eV/molecule.
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Cytokinin is an important endogenous hormone in plants performing a wide spectrum of biological roles. The type-A response regulators (RRAs) are primary cytokinin response genes, which are important components of the cytokinin signaling pathway and are involved in the regulation of plant growth and development. By analysis of the whole genome sequence of wheat, we identified 20 genes encoding RRAs which were clustered into eight homologous groups. The gene structure, conserved motifs, chromosomal location, and cis-acting regulatory elements of the TaRRAs were analyzed. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that the expression levels of most of the TaRRAs increased rapidly on exogenous cytokinin application. Moreover, the TaRRA family members displayed different expression profiles under the stress treatments of drought, salt, cold, and heat. This study provides valuable insights into the RRA gene family in wheat and promotes the potential application of these genes in wheat genetic improvement.
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As an efficient and economical way of dealing with organic pollutants, piezo-photocatalysis has attracted great interest. In this work, we demonstrated that ferroelectricity and Schottky heterojunction engineering could significantly enhance the piezo-photocatalytic activity of AgNbO3. The poled 20 mol % K+ doped AgNbO3 disclosed its superior piezo-photocatalytic activity of 0.131 min-1 for 10 mg·L-1 RhB, which is 7.8 times of the pristine one under the condition of illumination only. The designed piezo-photocatalyst also exhibited good piezo-photocatalytic stability after four cycles. These merits are attributed to the built-in electric field associated with the large spontaneous polarization and low coercive field originated from the stable ferroelectric state after ferroelectricity engineering, plus with the electron trapper effect of the in situ precipitated metal Ag particles. Our work not only provides a promising piezo-photocatalyst for degrading organic contaminants but also paves a good way for developing high piezo-photocatalytic activity catalysts.
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The regulation of wheat protein quality is a highly complex biological process involving multiple metabolic pathways. To reveal new insights into the regulatory pathways of wheat glutenin synthesis, we used the grain-filling period wheat grains of the near-isogenic lines NIL-723 and NIL-1010, which have large differences in quality, to perform a combined transcriptome and proteome analysis. Compared with NIL-1010, NIL-723 had 1287 transcripts and 355 proteins with significantly different abundances. Certain key significantly enriched pathway were identified, and wheat quality was associated with alanine, aspartate and glutamate metabolism, nitrogen metabolism and alpha-linolenic acid metabolism. Differentially expressed proteins (DEPs) or Differentially expressed genes (DEGs) in amino acid synthesis pathways were upregulated primarily in the glycine (Gly), methionine (Met), threonine (Thr), glutamic acid (Glu), proline (proC), cysteine (Cys), and arginine (Arg) synthesis and downregulated in the tryptophan (trpE), leucine (leuC), citrulline (argE), and ornithine (argE) synthesis. Furthermore, to elucidate changes in glutenin in the grain synthesis pathway, we plotted a regulatory pathway map and found that DEGs and DEPs in ribosomes (RPL5) and the ER (HSPA5, HYOU1, PDIA3, PDIA1, Sec24, and Sec31) may play key roles in regulating glutenin synthesis. The transcriptional validation of some of the differentially expressed proteins through real-time quantitative PCR analysis further validated the transcriptome and proteomic results.
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Pão , Glutens/biossíntese , Proteoma , Transcriptoma , Triticum/metabolismo , Aminoácidos/biossíntese , Triticum/genéticaRESUMO
Developing novel functional materials with promising desired properties in enhancing energy conversion and lowering the catalytic reaction barriers is essential for the demand to solve the increasingly severe energy and environmental crisis nowadays. Metal oxide semiconductors (MOS) are widely used in the field of catalysis because of its excellent catalytic characteristics. Introduction of defects, in addition to the adjustment of composition and atomic arrangement in the materials can effectively improve the materials' catalytic performance. Especially, introducing oxygen vacancies (OVs) into the lattice structure of MOS has been developed as a facile route to improve MOS's optical and electronic transmission characteristics. And a large number of metal oxides with rich OVs have been served in oxygen reduction reaction (ORR), oxygen evolution reaction (OER), hydrogen evolution reaction (HER), carbon dioxide reduction reaction (CO2-RR) photo-degradation of organic pollutants, etc. This small review briefly outlines some preparation techniques to introduce OVs into MOS, and the characterization techniques to identify and quantify the OVs in MOS. The applications of OVs contained MOS especially in energy and environmental catalysis areas are also discussed. The effects of OVs types and concentrations on the catalytic performances are deliberated. Finally, the defective structure-catalytic property relationship is highlighted, and the future status and opportunities of MOS containing OVs in the catalytic field are suggested.