RESUMO
Several studies describe the immobilization of microbial lipases aiming to evaluate the mechanical/thermal stability of the support/enzyme system, the appropriate method for immobilization, acid and alkaline stability, tolerance to organic solvents and specificity of fatty acids. However, literature reviews focus on application of enzyme/support system in food technology remains scarce. This current systematic literature review aimed to identify, evaluate and interpret available and relevant researches addressing the type of support and immobilization techniques employed over lipases, in order to obtain products for food industry. Fourteen selected articles were used to structure the systematic review, in which the discussion was based on six main groups: (i) synthesis/enrichment of polyunsaturated fatty acids; (ii) synthesis of structured lipids; (iii) flavors and food coloring; (iv) additives, antioxidants and antimicrobials; (v) synthesis of phytosterol esters and (vi) synthesis of sugar esters. In general, the studies discussed the synthesis of the enzyme/support system and the characteristics: surface area, mass transfer resistance, activity, stability (pH and temperature), and recyclability. Each immobilization technique is applicable for a specific production, depending mainly on the sensitivity and cost of the process.
Assuntos
Enzimas Imobilizadas , Indústria Alimentícia , Estabilidade Enzimática , Enzimas Imobilizadas/metabolismo , Ésteres , Ácidos Graxos , SolventesRESUMO
Mannosylerythritol lipids (MELs) have attracted particular interest of medical, pharmaceutical, and cosmetic fields, due to their specific characteristics, including non-toxicity, easy biodegradability, and environmental compatibility. Therefore, this review aims to highlight recent findings on MEL biological properties, focusing on issues related to therapeutic applications. Among the main findings is that MELs can play a fundamental role due to their antimicrobial properties against several nosocomial pathogen microorganisms. Other remarkable biological properties of MELs are related to skincare, as antiaging (active agent), and in particular on recover of skin cells that were damaged by UV radiation. MEL is also related to the increased efficiency of DNA transfection in liposome systems. Regarding the health field, these glycolipids seem to be associated with disturbance in the membrane composition of cancerous cells, increasing expression of genes responsible for cytoplasmic stress and apoptosis. Moreover, MELs can be associated with nanoparticles, as a capping agent, also acting to increase the solubility and cytotoxicity of them. Furthermore, the differences in the chemical structure of MEL could improve and expand their biochemical diversity and applications. Such modifications could change their interfacial properties and, thus, reduce the surface tension value, enhance the solubility, lower critical micelle concentrations, and form unique self-assembly structures. The latest is closely related to molecular recognition and protein stabilization properties of MEL, that is, essential parameters for their effective cosmetical and pharmaceutical effects. Thus, this current research indicates the huge potential of MEL for use in biomedical formulations, either alone or in combination with other molecules.
Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Glicolipídeos/química , Glicolipídeos/farmacologia , Antibacterianos/química , Bactérias/patogenicidade , Cosméticos , Humanos , Micelas , TensoativosRESUMO
Mannosylerythritol lipids (MEL) are glycolipids mainly produced by pseudo-yeasts. These molecules present remarkable biological activities widely explored in many fields, including medicine, pharmaceuticals, and cosmetics. This review presents the main biological activity of MEL on the HL60, K562, B16, PC12, and skin cells. There is strong evidence that MEL changes the levels of glycosphingolipids of HL-60 lineage, which induce differentiation into granulocytic cells. Regarding B16 cells, MEL can trigger both apoptosis (10 µM) and cell differentiation (5 µM), in which the MEL concentration is related to each metabolic pathway. MEL can also trigger differentiation in PC12 cells due to the increase in the GalCer content. In this specific case, the effects are transient, and the differentiated cells are unstable and tend to apoptosis. MEL-B can particularly maintain skin hydration and moisture due to their self-assembled structures that resemble the tissue cells. Moreover, MEL-B repair aquaporin expression in the HaCaT keratinocytes damaged with UVA irradiation, whereas MEL-C suppresses the expression of COX-2 protein in fibroblasts, indicating that these glycolipids activate the cellular antioxidant mechanism. Recent findings denoted the anti-melanogenic activity of MEL since they suppress tyrosinase enzyme at mRNA levels in B16 and NHMs cells. MEL act effectively on mammalian cells; however, there is no clear pattern of their metabolic effects. Also, gene expression levels seem to be related to two main factors: chemical structure and concentration. However, the specific signaling cascades that are induced by MEL remain inconclusive. Thus, further investigations are vital to understanding these mechanisms clearly. KEY POINTS: ⢠The four MEL homologs promote different biological responses in mammalian cells. ⢠MEL modifies the pattern of glycosphingolipids in the plasma membrane of tumor cells. ⢠Activation/deactivation of phosphorylation of serine/threonine kinase proteins.