Abnormal fragile histidine triad (FHIT) expression in advanced cervical carcinoma: a poor prognostic factor.

The FHIT gene is a candidate tumor suppressor gene that has been implicated in the development of cervical carcinoma. We hypothesized that abnormal Fhit expression might be a poor prognostic factor for patients with cervical cancer. The tumors from 59 high-risk patients (stage II-III) were evaluated for abnormal Fhit expression by immunohistochemical staining. Abnormal Fhit expression (absent or reduced) was noted in 66% of the specimens. There was no statistical difference with respect to stage, performance status, para-aortic node metastasis, completion of therapy, grade, race, age, and HIV status between the normal and abnormal Fhit expression groups. The 3-year survival for patients whose tumors displayed normal Fhit expression versus abnormal Fhit expression was 74% versus 37%, respectively. Univariate analysis demonstrated a difference in survival that was statistically significant for age <55 years versus > or =55 years (P = 0.015), normal Fhit expression versus abnormal Fhit expression (P = 0.015), and stage II versus stage III (P = 0.033). Multivariate analysis showed that abnormal Fhit expression was a poor prognostic factor (P = 0.015).

Detection of human papillomavirus in cervical biopsies of summer camp ROTC cadets with abnormal papanicolaou smears.

STUDY OBJECTIVE: Human papillomavirus (HPV) infections may be the most common sexually transmitted disease (STD) among adolescents and young adults. A strong association exists for certain "high-risk" HPV-type cervical infections and subsequent evolution of cervical cancer and its precursor lesions. The objective of this study was to detect HPV in cervical biopsies of reserve officer training corps (ROTC) cadets attending a regional summer camp who had abnormal screening Papanicolaou (Pap) smears and to better define the spectrum of HPV oncogenic risk types for this population. It was hypothesized that HPV would be detected in the majority of cervical biopsies in keeping with the evolving concept of cervical cancer and its precursors as an STD and the central role of HPV in its development. DESIGN: On arrival for summer military camp training, college women cadets underwent physical examinations that included pelvic examinations for Pap smears. Study participants completed confidential questionnaires after informed consent was obtained. Subjects, who had abnormal Pap smears as defined by reports of atypical squamous cells of undetermined significance (ASCUS) or low- or high-grade squamous intra-epithelial lesions (LGSIL or HGSIL), underwent colposcopic evaluations with cervical biopsies. Subsequently, biopsies were processed for detection of HPV-specific DNA by gel electrophoresis, chemiluminescent oligonucleotide probing, and DNA-RNA hybridization methods after polymerase chain reaction (PCR) amplification. PARTICIPANTS: All female summer camp enrollees presented to the Madigan Army Medical Center Gynecology Clinic for complete physicals before the onset of the training program and were solicited for study inclusion. MAIN OUTCOME MEASURES: All colposcopic biopsy specimens were evaluated for the presence of HPV DNA and subtyped if positive. RESULTS: Representing 42 states, 95% (332) of eligible subjects participated. Mean age was 21.9 years. Racial composition included 70% white, 16% black, 4% Hispanic, 4% Asian, and 6% "other." Eighty-five percent had a history of hetero-sexual activity, with 17.7 years as the mean age at onset. Of the subjects, 12.6% reported a previous diagnosis of a sexually transmitted disease, and 7.8% (26 of 332) had abnormal Pap smears. Of these, colposcopic biopsy results were available for 25 of the 26 abnormal Pap smears. Ninety-six percent (24 of 25) of the colpobiopsies had detectable HPV. Eighty-eight percent (22 of 25) of the subjects with abnormal Pap smears had concurrent abnormal biopsy results. All of these had detectable HPV, confirmed by three different methods, and 29% had dual HPV infection. Sixty percent had "intermediate" or "high-risk" types identified by specific genotyping, 8% had "low-risk" types, and 28% had nontypable HPV. CONCLUSIONS: The data confirm that abnormal Pap smears in this young adult college population are almost always associated with HPV infection, a significant proportion of it being other than low-risk types. The prognostic significance of type-specific cervical HPV infection still needs to be better defined in relation to potential cofactors and host immune response. However, clinicians who provide primary gynecologic care to adolescents and young adults should be aware of the high correlation between abnormal Pap smears and HPV cervical infection and should follow up the patient with the potential risk in mind.

Further comments about infant circumcision.

Wellington data confirm the findings of a Christchurch study of infant circumcision with the added finding that social class (measured by mother's education and husband's socio-economic status) is significant in determining outcome. Women are subject to pressure from medical staff, not to have boys circumcised resulting in a lower actual circumcision rate (20 percent) than that anticipated six to eight weeks before delivery (37 percent).

Large cell neuroendocrine cervical carcinoma: a report of two cases and review of the literature.

BACKGROUND: Large cell neuroendocrine cervical carcinoma is a rare malignancy. These tumors appear to mimic the aggressive behavior of small cell neuroendocrine tumors. Metastasis and recurrent disease are common. Due to the low incidence of these tumors, optimal therapy has not been delineated. CASES: Two patients presented with large cell neuroendocrine cervical carcinoma, stage IB1 and IIA, at our institution from 1997 to 1999. We describe the clinical course for these two patients and review the relevant literature for the management of large cell cervical carcinoma. CONCLUSION: Unlike squamous cell carcinoma, early-stage large cell neuroendocrine tumors of the cervix are aggressive. Disease recurrences are frequent and distant metastasis is common. Multimodal therapy should be considered at the time of initial diagnosis.

Primary appendiceal malignancy mimicking advanced stage ovarian carcinoma: a case series.

BACKGROUND: Primary appendiceal malignancy metastatic to the ovaries is a rare condition that may mimic late stage ovarian cancer. This condition is rarely diagnosed preoperatively. CASES: Three patients referred to our institution from 1994 to 1999 for presumed late stage ovarian cancer were found to have primary appendiceal adenocarcinoma, adenocarcinoid, and mucinous cystadenocarcinoma metastatic to the ovaries at laparotomy. We describe the clinical course of these patients and review the relevant literature. CONCLUSION: It is important for the gynecologic oncologist to be aware of the clinicopathological features and surgical management of these malignancies, as the incidence, prognosis, and recommended treatment vary with histological subtype.