An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity.

Activation of the innate immune system via pattern recognition receptors (PRRs) is key to generate lasting adaptive immunity. PRRs detect unique chemical patterns associated with invading microorganisms, but whether and how the physical properties of PRR ligands influence the development of the immune response remains unknown. Through the study of fungal mannans, we show that the physical form of PRR ligands dictates the immune response. Soluble mannans are immunosilent in the periphery but elicit a potent pro-inflammatory response in the draining lymph node (dLN). By modulating the physical form of mannans, we developed a formulation that targets both the periphery and the dLN. When combined with viral glycoprotein antigens, this mannan formulation broadens epitope recognition, elicits potent antigen-specific neutralizing antibodies, and confers protection against viral infections of the lung. Thus, the physical properties of microbial ligands determine the outcome of the immune response and can be harnessed for vaccine development.

Theory of the cubic autoproduct and its utility for noisy direction of arrival estimationa).

Autoproducts are quadratic or higher products of frequency-domain acoustic fields that can mimic genuine fields at frequencies within or outside the original field's bandwidth. Past studies have found a variety of interesting autoproduct properties but have been limited to quadratic autoproducts. This paper presents cubic autoproduct theory and documents how noise suppression may be attained with the cubic frequency-difference autoproduct, a product of three frequency-domain acoustic fields. The cubic autoproduct's field equations, developed from the inhomogeneous Helmholtz equation, and analytical results in single- and two-path environments justify interpretating the cubic autoproduct as a pseudofield and underscore its similarities to the quadratic autoproducts. For nonzero field bandwidth, many frequency triplets satisfy the relationship for a single cubic autoproduct frequency. Thus, bandwidth averaging can lead to serendipitous noise suppression and is shown herein to facilitate field-phase-structure recovery from ideal free space fields corrupted by Gaussian noise. Cubic-autoproduct-based direction of arrival (DOA) estimation using signal and noise recordings collected in the ocean are found to be more accurate than conventional DOA estimates from the same data. In particular, cubic autoproduct results showed a 3-5 dB noise suppression advantage for 4- and 6-kHz direct- and reflected-path sounds broadcast 200 m to a four-element receiving array.

Coherence of the frequency-difference autoproduct deduced from high-frequency acoustic fields scattered from a rough sea surfacea).

The prevalence of random scattering from a rough ocean surface increases with increasing χ=kh cos θ, where k is the acoustic wavenumber, h is the root-mean-square surface height, and θ is the incidence angle. Generally, when χ≫1, coherence between incident and surface-scattered fields is lost. However, such coherence may be recovered when χ≫1 by considering the frequency-difference autoproduct of the surface-scattered field, a quadratic product of complex fields at nearby frequencies. Herein, the autoproduct's coherent reflection coefficient for χ> 20 is determined from surface-scattered sound fields obtained from 50 independent realizations of the rough ocean surface measured in pelagic waters off the coast of California in January 1992. The recordings were made with a source at a depth of 147 m that broadcasted 30 and 40 kHz signals to a single receiver 576 m away at depth of 66 m. An analytic formula for the coherent reflection coefficient of the frequency-difference autoproduct, based on the Kirchhoff approximation and a Gaussian surface autocorrelation function, compares favorably with measurements. Improved agreement with the single-receiver measurements is possible via a minor adjustment to the surface autocorrelation length. The adjustment identified here matches that determined previously from horizontal spatial coherence estimates utilizing the experiment's eight-element receiving array.

Coherent reflection recovery in scattering from the ocean surface using the frequency-difference autoproducta).

The coherence of rough sea-surface-scattered acoustic fields decreases with increasing frequency. The frequency-difference autoproduct, a quadratic product of acoustic fields at nearby frequencies, mimics a genuine field at the difference frequency. In rough-surface scattering, the autoproduct's lower effective frequency decreases the apparent surface roughness, restoring coherent reflection. Herein, the recovery of coherent reflection in sea surface scattering via the frequency-difference autoproduct is examined for data collected off the coast of New Jersey during the Shallow Water '06 (SW06) experiment. An acoustic source at depth 40 m and receiver at depth 24.3 m and range 200 m interrogated 160 independent realizations of the ocean surface. The root mean square surface height h was 0.167 m, and broadcast frequencies were 14-20 kHz, so that 2.5 ≤kh cos θ≤ 3.7 for acoustic wavenumber k and incidence angle θ. Measured autoproducts, constructed from scattered constituent fields, show significant coherent reflection at sufficiently low difference frequencies. Theoretical results, using the Kirchhoff approximation and a non-analytic surface autocorrelation function, agree with experimental findings. The match is improved using a numerical strategy, exploiting the relationship between autoproduct-based coherence recovery, the ocean-surface autocorrelation function, and the ocean-surface height spectrum. Error bars computed from Monte Carlo scattering simulations support the validity of the measured coherence recovery.

Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance.

BACKGROUND: Obesity and type 2 diabetes mellitus (T2DM) are associated with an increased risk of severe outcomes from infectious diseases, including coronavirus disease 2019. These conditions are also associated with distinct responses to immunization, including an impaired response to widely used severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccines. OBJECTIVE: We sought to establish a connection between reduced immunization efficacy via modeling the effects of metabolic diseases on vaccine immunogenicity that is essential for the development of more effective vaccines for this distinct vulnerable population. METHODS: A murine model of diet-induced obesity and insulin resistance was used to model the effects of comorbid T2DM and obesity on vaccine immunogenicity and protection. RESULTS: Mice fed a high-fat diet (HFD) developed obesity, hyperinsulinemia, and glucose intolerance. Relative to mice fed a normal diet, HFD mice vaccinated with a SARS-CoV-2 mRNA vaccine exhibited significantly lower anti-spike IgG titers, predominantly in the IgG2c subclass, associated with a lower type 1 response, along with a 3.83-fold decrease in neutralizing titers. Furthermore, enhanced vaccine-induced spike-specific CD8+ T-cell activation and protection from lung infection against SARS-CoV-2 challenge were seen only in mice fed a normal diet but not in HFD mice. CONCLUSIONS: The study demonstrated impaired immunity following SARS-CoV-2 mRNA immunization in a murine model of comorbid T2DM and obesity, supporting the need for further research into the basis for impaired anti-SARS-CoV-2 immunity in T2DM and investigation of novel approaches to enhance vaccine immunogenicity among those with metabolic diseases.

Localization of a remote source in a noisy deep ocean sound channel using phase-only matched autoproduct processing.

Long-range passive source localization is possible in the deep ocean using phase-only matched autoproduct processing (POMAP) [Geroski and Dowling (2021). J. Acoust. Soc. Am. 150, 171-182], an algorithm based on matched field processing that is more robust to environmental mismatch. This paper extends these prior POMAP results by analyzing the localization performance of this algorithm in the presence of environmental noise. The noise rejection performance of POMAP is assessed using both simulated and measured signal data, with noise data based on environmental noise measurements. Herein, signal and noise measurements are from the nominally one-year-long PhilSea10 ocean acoustic propagation experiment. All signals were recorded from a single moored source, placed near the ocean sound channel 129.4 km away from a nearly water-column-spanning distributed vertical line array. The source transmitted linear frequency modulated chirps with nominal bandwidth from 200 to 300 Hz. The noise measurements used in this study were collected in the months after this source stopped transmitting, and synthetic samples of noise are calculated based on the characteristics of this measured noise. The effect that noise rejection algorithms have on the source localization performance of POMAP is also evaluated, but only 1 dB of performance improvement is achieved using these.

A Precision Adjuvant Approach to Enhance Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Vaccines Optimized for Immunologically Distinct Vulnerable Populations.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused significant mortality, especially among older adults whose distinct immune system reflects immunosenescence. Multiple SARS-CoV-2 vaccines have received emergency use authorization and/or licensure from the US Food and Drug Administration and throughout the world. However, their deployment has heighted significant limitations, such by age-dependent immunogenicity, requirements for multiple vaccine doses, refrigeration infrastructure that is not universally available, as well as waning immunity. Thus, there was, and continues to be a need for continued innovation during the pandemic given the desire for dose-sparing, formulations stable at more readily achievable temperatures, need for robust immunogenicity in vulnerable populations, and development of safe and effective pediatric vaccines. In this context, optimal SARS-CoV-2 vaccines may ultimately rely on inclusion of adjuvants as they can potentially enhance protection of vulnerable populations and provide dose-sparing effects enabling single shot protection.

Precision Vaccine Adjuvants for Older Adults: A Scoping Review.

Older adults, defined as those ≥60 years of age, are a growing population vulnerable to infections including severe acute respiratory syndrome coronavirus 2. Although immunization is a key to protecting this population, immunosenescence can impair responses to vaccines. Adjuvants can increase the immunogenicity of vaccine antigens but have not been systematically compared in older adults. We conducted a scoping review to assess the comparative effectiveness of adjuvants in aged populations. Adjuvants AS01, MF59, AS03, and CpG-oligodeoxynucleotide, included in licensed vaccines, are effective in older human adults. A growing menu of investigational adjuvants, such as Matrix-M and CpG plus alum, showed promising results in early phase clinical trials and preclinical studies. Most studies assessed only 1 or 2 adjuvants and no study has directly compared >3 adjuvants among older adults. Enhanced preclinical approaches enabling direct comparison of multiple adjuvants including human in vitro modeling and age-specific animal models may derisk and accelerate vaccine development for older adults.

Acceptability of a Fentanyl Vaccine to Prevent Opioid Overdose and Need for Personalized Decision-Making.

BACKGROUND: The opioid epidemic worsened during the coronavirus disease 2019 (COVID-19) pandemic. Synthetic opioids (primarily fentanyl) comprise the most common drugs involved in overdose (OD) death. A vaccine that blocks fentanyl from reaching the brain to prevent OD is under development, and insight is needed into its acceptability. METHODS: Using a semi-structured interview guide, persons with opioid use disorder (OUD), family, professionals, and the public were interviewed about attitudes and concerns regarding a fentanyl vaccine. Reactions to fictional clinical vignettes of persons at risk of OUD because of pain and/or substance use histories were collected, analyzed, and quantified for favorability. Interviews were transcribed, coded, and analyzed thematically. RESULTS: Among N = 64 participants, (70.3% female, average age 32.4 years), attitudes were favorable toward a fentanyl vaccine, with preference for lifelong durability (76% of n = 55 asked). Perceived benefits centered on the potential for a life-saving intervention, suffering averted, healthcare dollars saved, and the utility of a passive harm reduction strategy. Concerns centered on uncertainty regarding vaccine safety, questions about efficacy, worry about implications for future pain management, stigma, and need for supportive counseling and guidance to personalize decision making. Reactions to vignettes revealed complex attitudes toward fentanyl vaccination when considering recipient age, health history, and future risks for addiction and pain. CONCLUSIONS: Positive responses to a fentanyl vaccine were found along with appreciation for the complexity of a vaccine strategy to prevent OD in the setting of pain and uncertain durability. Further research is needed to elucidate operational, ethical, and communications strategies to advance the model.

Recovery of coherent reflection from rough-surface scattered acoustic fields via the frequency-difference autoproduct.

The acoustic field reflected from a random rough surface loses coherence with the incident field in the Kirchhoff approximation as kh cos θ increases, where k is the incident field wavenumber, h is the root mean square roughness height, and θ is the incidence angle. Thus, for fixed rough-surface properties and incidence angle, a reflected field at lower wavenumber should retain more coherence. Recent results suggest that the frequency-difference autoproduct formed from complex acoustic field amplitudes at two nearby frequencies can recover acoustic information at the difference of those frequencies even when the difference frequency is below the recorded field's bandwidth. Herein analytical, computational, and experimental results are presented for the extent to which the frequency-difference autoproduct recovers coherence from randomly rough-surface-scattered constituent fields that have lost coherence. The analytical results, developed from the Kirchhoff approximation and formal ensemble averaging over randomly rough surfaces with Gaussian height distributions and Gaussian correlation functions, indicate that the coherence of the rough-surface-reflected frequency-difference autoproduct depends on the surface correlation length and Δkh cos θ, where Δk is the difference of the autoproduct's constituent field wavenumbers. These results compare favorably with Monte Carlo simulations of rough surface scattering, and with laboratory experiments involving long surface correlation lengths where 1 ≤kh cos θ≤ 3.

The sixth revolution in pediatric vaccinology: immunoengineering and delivery systems.

Infection is the predominant cause of mortality in early life, and immunization is the most promising biomedical intervention to reduce this burden. However, very young infants fail to respond optimally to most vaccines currently in use, especially neonates. In 2005, Stanley Plotkin proposed that new delivery systems would spur a new revolution in pediatric vaccinology, just as attenuation, inactivation, cell culture of viruses, genetic engineering, and adjuvantation had done in preceding decades. Recent advances in the field of immunoengineering, which is evolving alongside vaccinology, have begun to increasingly influence vaccine formulation design. Historically, the particulate nature of materials used in many vaccine formulations was empiric, often because of the need to stabilize antigens or reduce endotoxin levels. However, present vaccine delivery systems are rationally engineered to mimic the size, shape, and surface chemistry of pathogens, and are therefore often referred to as "pathogen-like particles". More than a decade from his original assessment, we re-assess Plotkin's prediction. In addition, we highlight how immunoengineering and advanced delivery systems may be uniquely capable of enhancing vaccine responses in vulnerable populations, such as infants. IMPACT: Immunoengineering and advanced delivery systems are leading to new developments in pediatric vaccinology. Summarizes delivery systems currently in use and development, and prospects for the future. Broad overview of immunoengineering's impact on vaccinology, catering to Pediatric Clinicians and Immunologists.

Robust long-range source localization in the deep ocean using phase-only matched autoproduct processing.

Passive source localization in the deep ocean using array signal processing techniques is possible using an algorithm similar to matched field processing (MFP) that interrogates a measured frequency-difference autoproduct instead of a measured pressure field [Geroski and Dowling, J. Acoust. Soc. Am. 146, 4727-4739 (2019)]. These results are extended herein to a new MFP-style algorithm, phase-only matched autoproduct processing, that is more robust at source-array ranges as large as 225 km. This new algorithm is herein described and compared to three existing approaches. The performance of all four techniques is evaluated using measured ocean propagation data from the PhilSea10 experiment. These data nominally span a 12-month period; include six source-array ranges from 129 to 450 km; and involve signals with center frequencies between 172.5 and 275 Hz, and bandwidths of 60 to 100 Hz. In all cases, weight vectors are calculated assuming a range-independent environment using a single sound-speed profile measured near the receiving array. The frequency-differencing techniques considered here are capable of localizing all six sources, with varying levels of consistency, using single-digit-Hz difference frequencies. At source-array ranges up to and including 225 km, the new algorithm requires fewer signal samples for success and is more robust to the choice of difference frequencies.

Measurements of the correlation of the frequency-difference autoproduct with acoustic and predicted-autoproduct fields in the deep ocean.

Frequency-domain spatial-correlation analysis of recorded acoustic fields is typically limited to the bandwidth of the recordings. A previous study [Lipa, Worthmann, and Dowling (2018) J. Acoust. Soc. Am. 143(4), 2419-2427] suggests that limiting such analysis to in-band frequencies is not strictly necessary in a Lloyd's mirror environment. In particular, below-band field information can be retrieved from the frequency-difference autoproduct, a quadratic product of measured complex pressure-field amplitudes from two nearby frequencies. The frequency-difference autoproduct is a surrogate field that mimics a genuine acoustic field at the difference frequency. Here, spatial-correlation analysis is extended to deep-ocean acoustic fields measured during the PhilSea10 experiment. The frequency-difference autoproduct, at difference frequencies from 0.0625 to 15 Hz, is determined from hundreds of Philippine Sea recordings of 60 or 100 Hz bandwidth signals with center frequencies from 172.5 to 275 Hz broadcast to a vertical receiving array 129-450 km away. The measured autoproducts are cross correlated along the array with predicted acoustic fields and with predicted autoproduct fields at corresponding below-band frequencies. Stable measured cross correlations as high as 80%-90% are found at the low end of the investigated difference-frequency range, with consistent correlation loss due to mismatch at the higher below-band frequencies.

Effect of green waste and lime amendments on biostabilisation, physical-chemical and microbial properties of the composted fine fraction of residual municipal solid waste.

Implementation of guidelines to reduce the amount of biodegradable municipal waste (BMW) sent to landfill has created a need in the waste-management industry to investigate possible methods of accelerating biostabilisation of residual BMW. The effect of commercially feasible manipulations (lime and green waste (GW)) on the rate of biostabilisation of the fine (<20 mm) fraction of residual BMW was investigated. The physical and chemical attributes of the composted wastes were measured, and their bacterial communities profiled using traditional culture-based methods. In addition, ammonia-oxidising microbes were monitored during the biostabilisation process using molecular profiling methods. Addition of GW accelerated biostabilisation, reduced conductivity and increased the levels of ammonia-oxidising bacterial (AOB) and archaeal (AOA) genes. The best stability was noted in the dual (Lime + GW) treatment, which was under the limit of 13 mmol O2 kg DM-1 h-1 recommended by the Irish compost standard. Biostabilised wastes met recommendations for source-segregated compost for pH (6-8) and pathogens (E. coli and Salmonella), but not heavy metals, indicating their unsuitability for uses other than landfill cover. Levels of AOA genes (log 3-6 g-1 DM) were higher than AOB (log 1-6 g-1 DM, indicating AOA may contribute more to potential ammonia oxidation in residual BMW composting.

Toward precision adjuvants: optimizing science and safety.

PURPOSE OF REVIEW: The gradual replacement of inactivated whole cell and live attenuated vaccines with subunit vaccines has generally reduced reactogenicity but in many cases also immunogenicity. Although only used when necessary, adjuvants can be key to vaccine dose/antigen-sparing, broadening immune responses to variable antigens, and enhancing immunogenicity in vulnerable populations with distinct immunity. Licensed vaccines contain an increasing variety of adjuvants, with a growing pipeline of adjuvanted vaccines under development. RECENT FINDINGS: Most adjuvants, including Alum, Toll-like receptor agonists and oil-in-water emulsions, activate innate immunity thereby altering the quantity and quality of an adaptive immune response. Adjuvants activate leukocytes, and induce mediators (e.g., cytokines, chemokines, and prostaglandin-E2) some of which are biomarkers for reactogenicity, that is, induction of local/systemic side effects. Although there have been safety concerns regarding a hypothetical risk of adjuvants inducing auto-immunity, such associations have not been established. As immune responses vary by population (e.g., age and sex), adjuvant research now incorporates principles of precision medicine. Innovations in adjuvant research include use of human in vitro models, immuno-engineering, novel delivery systems, and systems biology to identify biomarkers of safety and adjuvanticity. SUMMARY: Adjuvants enhance vaccine immunogenicity and can be associated with reactogenicity. Novel multidisciplinary approaches hold promise to accelerate and de-risk targeted adjuvant discovery and development. VIDEO ABSTRACT: http://links.lww.com/MOP/A53.

High-resolution acoustic localization of changes in spatially-distributed coherent sources for structural health monitoring.

Localization of acoustic sources is a common remote sensing goal. When multiple sources are present and coherent, high-resolution localization typically becomes more challenging. The spectral estimation method with additive noise (SEMWAN) is an existing technique for high-resolution localization of incoherent monopole sources in low-signal-to-noise environments. SEMWAN utilizes a reference measurement to incoherently suppress background noise, but its performance suffers in applications involving spatially-distributed coherent sources, such as like a vibrating plate. However, by subtracting a reference measurement and using subarray averaging, SEMWAN can be extended to localization of small changes in distributed coherent sources. This revised approach, the spectral estimation method with coherent background removal (SEMCBR), permits remote acoustic localization of damage in a vibrating structure. A simple multi-source experiment using an 8-by-8 planar square microphone array with 6-cm spacing in both horizontal directions was performed to validate SEMCBR at a frequency of 5.0 kHz. Additional SEMCBR experimental results are reported for the same array placed 1.0 m above a 30 cm × 30 cm vibrating plate with and without damage. Cuts, boundary failures and delamination of a composite plate were successfully located with conventional spherical-wave beamforming and SEMCBR using a 0.1 to 6.0 kHz bandwidth. However, SEMCBR provides 4 to 6 times better resolution.

The effects of refraction and caustics on autoproducts.

Quadratic products of complex amplitudes from acoustic fields with nonzero bandwidth, denoted "autoproducts," can mimic acoustic fields at frequencies lower or higher than the bandwidth of the original field. While this mimicry has been found to be very promising for a variety of signal processing applications, its theoretical extent has, thus far, only been considered under the most elementary ray approximation. In this study, the combined effects of refraction and diffraction are considered in environments where refraction causes neighboring rays to cross and form caustics. Acoustic fields on and near caustics are not well-predicted by elementary ray-acoustic theory. Furthermore, caustics introduce frequency dependence to the nearby acoustic field and a phase shift on the acoustic waves that passes through them. The effects these caustics have on autoproducts is assessed here using two simple, range-independent waveguides with index of refraction (n) profiles that are n2-quadratic and n2-linear. It is found that in multipath regions where rays have passed through differing numbers of caustics, the ability of autoproducts to mimic out-of-band fields is substantially hindered.

Autoproducts in and near acoustic shadow zones created by barriers.

The autoproducts are nonlinear mathematical constructs developed from acoustic fields with non-zero bandwidth. When averaged through the field's bandwidth, the autoproducts may mimic a genuine acoustic field at frequencies that are lower or higher than the original field's bandwidth. The resulting opportunity to extend signal processing to user-selectable below- or above-band frequencies is intriguing for many signal processing algorithms. Based on prior work, the limitations of the autoproducts' mimicry of out-of-band fields are understood when the in-band acoustic field is well-represented by ray acoustics. Thus, the focus in this study is on autoproducts in acoustic shadow zones behind barriers containing only diffracted acoustic fields where a sum of ray-path contributions is not an adequate field description. Diffraction is expected to be a detriment to autoproduct techniques due to its sensitivity to frequency. Two ideal shadow-zone environments with exact analytic Helmholtz-equation solutions are considered: Sommerfeld's half-plane problem, also known as knife-edge diffraction, and Mie scattering from a sphere with ka = 40, where k is the wavenumber and a is the sphere's radius. With the exception of the shadow regions, autoproducts experience only mild degradation in field-mimicry performance when compared to what the ray-based theory would predict.

Long-range frequency-difference source localization in the Philippine Sea.

Matched field processing (MFP) refers to a variety of source localization schemes for known complicated environments and involves matching measured and calculated (replica) fields to identify source locations. MFP may fail for several reasons, most notably when the calculated fields are insufficiently accurate. This error commonly prevents MFP-based long-range (>100 km) source localization in the deep ocean (from 5 to 6 km depth) for signal frequencies of hundreds of Hz, even when extensive high-signal-to-noise ratio field measurements are available. Recently, below-band MFP utilizing the frequency-difference autoproduct [Worthmann, Song, and Dowling (2015). J. Acoust. Soc. Am, 138(6), 3549-3562] achieved some shallow-ocean localization success at a 3 km source-to-array range with signal frequencies in the tens of kHz. The performance of this technique, when extended to matching the measured frequency-difference autoproduct with a composite mode-ray replica, is described here for deep ocean source localization. The ocean propagation data come from the PhilSea10 experiment and involve source-to-array ranges from 129 to 379 km and nominal 100-Hz-bandwidth signals having center frequencies from 250 to 275 Hz. Based on an incoherent average of five signal samples, the frequency-difference technique was 90%-100% successful at four different source-to-array ranges using single-digit-Hz difference frequencies.

Remote acoustic detection of cuts in a vibrating plate with stochastic input forcing in a reverberant environment.

A mechanical structure subject to vibratory forcing will often radiate sound. When remotely recorded, this sound depends on the vibratory forcing, the structure's frequency response function, and the structure-to-receiver acoustic propagation. Thus, successful remote acoustic detection of changes in a structure's vibration response between baseline and subsequent test recordings may require compensation for possible baseline-to-test changes in vibratory forcing and acoustic propagation. Compensation schemes for unknown structural forcing in an unknown reverberant environment that allow such remote detection are described here and found to be successful when the random forcing has a consistent power spectrum and the structure-radiated sound is recorded with an array of receivers. In particular, experimental results are presented for remote acoustic detection of 13-76 mm cuts in a vibrating 0.30-m-square by 3-mm-thick edge-clamped aluminum plate subject to 0.1-2 kHz base excitation in a reverberant laboratory. Radiated sound from the plate is recorded remotely with a 15-element microphone array and processed with the synthetic time reversal blind deconvolution algorithm to compensate for unknown reverberation. Cut detection success is compared for frequency-sweep and random-input forcing when this forcing is known and unknown, and when there are plate-to-array geometric changes between the baseline and test measurements.