RESUMO
The prevalence of Toxoplasma gondii was investigated on a poorly managed pig farm in Maryland. Serum and tissue samples from 48 of the 100 pigs on the farm were available for T. gondii evaluation. Serological testing was performed using both ELISA and the modified agglutination test (MAT). Antibodies to T. gondii were detected by ELISA in 12 of 48 animals, while antibodies were detected in 34 of 48 pigs by MAT with titers of 1:10 in 1, 1:20 in 4, 1:40 in 7, 1:80 in 3, 1:160 in 8, 1:320 in 3, 1:640 in 4, and 1:1,280 in 4. Hearts of 16 pigs with MAT titers of 1:10 or higher were bioassayed for T. gondii in cats; 11 cats shed T. gondii oocysts. Hearts of 22 pigs were autolyzed and bioassayed only in mice; T. gondii was isolated from 3 of these 22 pigs. Genetic typing of the 14 T. gondii isolates using the SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico loci revealed 4 genotypes; 10 isolates belonged to type II lineage (genotypes 1 and 2), 3 belonged to genotype 3, and 1 belonged to genotype 4. Genotype 1 and 2 have type II alleles at all genetic loci, except the former has type II allele and the latter has a type I allele at locus Apico. Both genotypes 1 and 2 are considered to belong to the clonal type II lineages. Genotype 3 and 4 are nonclonal isolates. Results document high prevalence of T. gondii in pigs on a farm in Maryland.
Assuntos
Doenças Endêmicas/veterinária , Doenças dos Suínos/epidemiologia , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/epidemiologia , Testes de Aglutinação/veterinária , Alelos , Animais , Anticorpos Antiprotozoários/sangue , Bioensaio , Gatos , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Genótipo , Coração/parasitologia , Masculino , Maryland/epidemiologia , Camundongos , Prevalência , Suínos , Doenças dos Suínos/parasitologia , Toxoplasma/classificação , Toxoplasma/genética , Toxoplasma/imunologia , Toxoplasmose Animal/parasitologiaRESUMO
Pigs infected with the zoonotic parasite Trichinella spiralis were detected on a farm in Maryland during an animal welfare investigation. Sera and/or tissues were collected from 49 pigs and three pig carcasses (7 weeks of age to adult, mixed sex). The tissues were tested for the presence of T. spiralis muscle larvae (ML) by tissue digestion, and the sera were tested for the presence of anti-Trichinella antibodies by ELISA. Seventeen of 50 (34%) pigs were infected with T. spiralis based on tissue digestion. Of these 17 pigs, sera were collected from 16; nine were serologically positive, three sera had OD values that were very close to the positive cut-off (0.30), but were still negative, and four were negative (suggesting that they had become infected within a few weeks of testing). All pigs that tested negative by tissue digestion for ML were also ELISA negative. The farm was subsequently depopulated of pigs. Six months later, testing of trapped scavenging mammals in the farm environment demonstrated that 41% were infected with T. spiralis. After 12 months, 10% of trapped animals were T. spiralis positive, and after 18 months, T. spiralis could not be detected in the scavenging mammal population surrounding the farm. Results of the study suggest that T. spiralis, typically transmitted in the peridomestic rat-pig-human cycle in the US, was not maintained in scavenging mammals in the absence of infected pigs.
Assuntos
Gambás/parasitologia , Guaxinins/parasitologia , Doenças dos Suínos/parasitologia , Trichinella spiralis/isolamento & purificação , Triquinelose/transmissão , Triquinelose/veterinária , Criação de Animais Domésticos , Animais , Animais Selvagens/parasitologia , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/isolamento & purificação , Doenças Transmissíveis/transmissão , Reservatórios de Doenças , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/veterinária , Humanos , Maryland/epidemiologia , Prevalência , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/prevenção & controle , Doenças dos Suínos/transmissão , Triquinelose/epidemiologia , Triquinelose/parasitologia , Triquinelose/prevenção & controleRESUMO
Recovery of behavioural and sensory function was examined following unilateral pedal nerve crush in Aplysia californica. Nerve crush that transected all axons connecting the tail to the central nervous system (CNS) eliminated the ipsilateral tail-evoked siphon reflex, whose sensory input travels in the crushed tail nerve (p9). The first reliable signs of recovery of this reflex were observed within 1 week, and most animals displayed tail-evoked siphon responses within 2 weeks. Wide-dynamic-range mechanosensory neurons with somata in the ventrocaudal (VC) cluster of the ipsilateral pleural ganglion exhibited a few receptive fields (RFs) on the tail 3 weeks after unilateral pedal nerve crush, indicating that the RFs had either regenerated or been reconnected to the central somata. These RFs were smaller and sensitized compared with corresponding RFs on the contralateral, uncrushed side. Centrally conducted axon responses of VC sensory neurones to electrical stimulation distal to the nerve crush site did not reappear until at least 10 days after the crush. Because the crush site was much closer to the CNS than to the tail, the failure of axon responses to be restored earlier than the behavioural responses indicates that early stages of reflex recovery are not due to regeneration of VC sensory neurone axons into the tail. Following nerve crush, VC sensory neurones often could be activated by stimulating central connectives or peripheral nerves that do not normally contain the sensory neurone's axons. These results suggest that recovery of behavioral function after nerve injury involves complex mechanisms, including regenerative growth of axotomized VC sensory neurones, sensitization of regenerating RFs and sprouting of VC sensory neurone fibres within the CNS. Furthermore, the rapidity of behavioural recovery indicates that its initial phases are mediated by additional mechanisms, perhaps centripetal regeneration of unidentified sensory neurones having peripheral somata, or transient reconnection of proximal and distal stumps of axotomized VC cells.
Assuntos
Aplysia/fisiologia , Axônios/fisiologia , Vias Neurais/fisiologia , Neurônios Aferentes/fisiologia , Sistema Nervoso Periférico/fisiologia , Animais , Encéfalo/fisiologia , Mecanorreceptores/fisiologia , Compressão Nervosa , Sistema Nervoso Periférico/lesõesRESUMO
Morphological methods were used to examine injury-induced growth of peripheral and central axons of nociceptive mechanosensory neurones in the ventrocaudal (VC) clusters of the pleural ganglia of Aplysia californica. Pedal nerve crush transected all axons in the nerve while leaving the overlying sheath largely intact. Immunohistochemical staining was performed with an antibody to a sensory-neurone-specific peptide, sensorin-A. Following bilateral crush of pedal nerve p9, which innervates the tail, sensorin-A immunofluorescence was lost distal to the crush site within 2 days. Fine immunopositive fibres began to invade the crush region within 5 days. These fibres arborized in the crush region and gradually extended down the crushed nerve. Immunopositive fibres were found near the tail within 3 weeks. Similar results were obtained after injecting individual sensory neurone somata in the tail/p9 region of the VC cluster with biocytin. Biocytin injections and horseradish peroxidase injections 3 weeks after ipsilateral pedal nerve crush revealed new fibres projecting rostrally from the tail/p9 region of the VC cluster and entering the pleural-cerebral and pleural-abdominal connectives. Such projections were never observed in control, uncrushed preparations. These results demonstrate that nerve injury triggers extensive growth of both peripheral and central processes of the VC sensory neurones.
Assuntos
Aplysia/fisiologia , Axônios/fisiologia , Sistema Nervoso Central/fisiologia , Regeneração Nervosa/fisiologia , Neurônios Aferentes/fisiologia , Sistema Nervoso Periférico/fisiologia , Animais , Imunofluorescência , Peroxidase do Rábano Silvestre , Lisina/análogos & derivados , Mecanorreceptores/fisiologia , Compressão Nervosa , Neuropeptídeos/análise , Sistema Nervoso Periférico/lesões , Cauda/inervaçãoRESUMO
Axon regeneration after injury and long-term alterations associated with learning both require protein synthesis in the neuronal cell body, but the signals that initiate these changes are largely unknown. Direct evidence that axonal injury activates molecular signals in the axon was obtained by injecting axoplasm from crushed or uncrushed nerves into somata of sensory neurons with uncrushed axons. Those injected with crush axoplasm behaved as if their axons had been crushed, exhibiting increases in both repetitive firing and spike duration, and a decrease in spike afterhyperpolarization 1 d after injection. Because similar changes occur in the same cells after learning, these data suggest that some of the long-lasting adaptive changes that occur after injury and learning may be induced by common axoplasmic signals. Since the signals in axoplasm must be conveyed to the cell soma, we have begun to test the hypothesis that at least some of these signals are proteins containing a nuclear localization signal (NLS). Axoplasmic proteins at the crush site and those that accumulated at a ligation proximal to the crush were probed with an antibody to an amino acid sequence (sp) containing a NLS that provides access to the retrograde transport/nuclear import pathway. One protein, sp97, displayed properties expected of an axonal injury signal: it responded to injury by undergoing an anterograde-to-retrograde change in movement and, when the ligation was omitted, it was transported to the cell bodies of the injured neurons.