Comparative study on the effect of dietary ß-carotene and phycocyanin extracted from Spirulina platensis on immune-oxidative stress biomarkers, genes expression and intestinal enzymes, serum biochemical in Nile tilapia, Oreochromis niloticus.

The current trial investigated the roles of ß-carotene and phycocyanin extracted from Spirulina platensis on growth, serum biochemical, digestive enzymes, antioxidant defense, immune responses, and immune gene expression in Nile tilapia (Oreochromis niloticus). Fish (1.52 ± 0.10 g) were randomly stocked to three treatments with three replicates (12 fish per replicate) in nine aquaria (60 L glass aquarium for each), and reared for 70-days. Three tested diets were formulated to be isonitrogenous and isolipidic, and were offered for experimental fish until ad-libitum three times daily at 09:00 a.m., 11.00 a.m. and 3:00 p.m. The first diet (control) was without supplementation. About 50 mg ß-carotene and 50 mg phycocyanin kg-1 were supplemented to the other experimental diets, respectively. Results indicated that feed intake was not (P > 0.05) differ among experimental diets. Compared to control diet wight gain and specific growth rate were significantly (P < 0.05) in fish fed diet containing ß-carotene, while, the highest weight gain and the best FCR were detected in phycocyanin diet. Survival fish among treatments was significantly (P < 0.05) differ and the highest survival rate was showed in fish fed diet supplemented with phycocyanin. Either ß-carotene or phycocyanin significantly (P < 0.05) improved the intestinal digestive enzymes compared with control diet, where the highest values of chymotrypsin, trypsin, lipase and amylase were noticed in fish fed phycocyanin. Diets supplemented with ß-carotene and phycocyanin significantly (P < 0.05) improved hematology parameters contents compared with to the control diet, and the best contents were detected in fish fed diet supplemented with phycocyanin. The highest significant (P < 0.05) phagocytic, lysozyme, immunoglobulin M (IgM), superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and total antioxidant capacity (T-AOC) activities were recorded in diet supplemented with phycocyanin. The transcripts of interferon gamma and interleukin 1ß genes were (P < 0.05) up-regulated in the liver of fish fed diet supplemented with ß-carotene and phycocyanin, but expression of HSP70 gene down-regulated in fish fed ß-carotene and phycocyanin containing diet compared control. The highest gene expression of the interferon gamma and interleukin 1ß was observed in fish fed phycocyanin.

The potential benefits of orange peels derived pectin on serum and skin mucus immune parameters, antioxidant defence and growth performance in common carp (Cyprinus carpio).

This study was performed to determine the effects of pectin derived from orange peel (PDOP) on growth performance, antioxidant enzyme activity and serum and skin mucus immune response of common carp (Cyprinus carpio). Common Carp (16.94 ± 0.03 g) were distributed into 12 tanks representing four treatments repeated in triplicates. Four diets were prepared to contain four levels of PDOP as follows: 0 (control), 0.5, 1, and 2% PDOP. Growth and immunological parameters as skin mucus lysozyme activity (SMLA) and total immunoglobulin (SMTIg), serum total immunoglobulin (STIg), serum peroxidase activities (SPA), Catalyse activity (CAT), DPPH radical scavenging activity, specific growth rate (SGR), weight gain (WG), final weight (FW), and feed conversion ratio (FCR) were assessed. Fish fed diets supplemented with PDOP showed an improvement of SGR, WG, FW, and FCR (P < 0.05). In terms of skin mucus immunological parameters, dietary inclusion of pectin significantly (P < 0.05) increased SMTIg. Likewise, carps fed either 1 or 2% PDOP showed notable enhancement of SMLA. In the case of serum immune parameters and antioxidant defence, carps in 1% PDOP treatment showed significantly (P < 0.05) higher SPA and CAT compared to fish fed either control diet or 0.5% OPDP. Additionally, no significant change (P > 0.05) was found in SPA and CAT of fish fed either 1% PDOP or 2% PDOP. Also, no significant (P > 0.05) difference was noticed between treated groups and control in the case of STIg. Furthermore, no significant differences were observed in DPPH radical activity among treatments (P > 0.05). Overall, these results suggested that inclusion of PDOP in common carp diet can beneficially affect growth and immune response.

Nano Iron Versus Bulk Iron Forms as Functional Feed Additives: Growth, Body Indices, Hematological Assay, Plasma Metabolites, Immune, Anti-oxidative Ability, and Intestinal Morphometric Measurements of Nile tilapia, Oreochromis niloticus.

The current study aimed to compare the utilization efficiency of iron (Fe) feed additives from either bulk or nano sources in Nile tilapia, Oreochromis niloticus diets on growth, haematological, immunity, anti-oxidative, and intestinal topography capacities. Five isonitrogenous and isoenergetic diets were performed; the basal diet served as a control with no Fe added, whereas the experimental diets were shaped by adding bulk-Fe2O3 and nano-Fe2O3 to the basal diet to preserve Fe levels at 0.2 and 0.4 mg kg-1, respectively. Results indicated that superior growth performance was recorded in fish-fed diets supplemented with 0.4 nano-Fe2O3 mg kg-1 diet. In addition, the highest (P ≤ 0.05) survival rate, absorption area of villous (AAV), mucosal to serosal amplification ratio (MSR), and villi parameters (height and width) were noticed in fish fed diet enrichment with either bulk or nano-Fe2O3 source. However, the superiority observed in nano-Fe2O3 fish groups. Also, the highest values of plasma albumin, total protein, high-density lipoprotein cholesterol (HDL-C), white blood cells (WBCs), and lymphocyte absolute count (LYM) (P ≤ 0.05) recorded in fish fed a diet supplemented with nano-Fe2O3 versus the basal diet. Moreover, the highest values of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and plasma lysozyme activity (P ≤ 0.05) were observed in fish fed 0.4 mg/kg-1 nano-Fe2O3, while the lowest value was recorded in fish fed the control diet. The best value of malondialdehyde activity (P ≤ 0.05) recorded in a fish-fed diet supplemented with 0.4 mg/kg-1 nano-Fe2O3. The current findings emphasize the importance of including Fe to improve fish growth, immunity, antioxidant capabilities, and intestinal structure, primarily with a nano-Fe source, which demonstrated a more effective function in satisfying Nile tilapia dietary Fe requirements and improving the aforementioned parameters.

Evaluation of Different Copper Sources in Nile Tilapia Diets: Growth, Body Indices, Hematological Assay, Plasma Metabolites, Immune, Anti-Oxidative Ability, and Intestinal Morphometric Measurements.

The goal of the current study was to compare how well Nile tilapia, Oreochromis niloticus, utilized copper (Cu) as bulk and nano sources by evaluating fish growth, body indices, hematological assays, plasma metabolites, immune and anti-oxidative abilities, and intestinal morphometric measurements. The basal diet served as a control, with no Cu added, whereas the experimental diets were formed by adding bulk-Cu and nano-Cu to the basal diet to keep Cu levels at 3 and 6 mg kg-1, respectively, in both Cu sources. Tilapia (9.10 ± 0.014 g) were fed the control diet and four experimental diets for 12 weeks. Results indicated that growth, feed utilization, and body indices demonstrated a substantial improvement (P ≤ 0.05) in tilapia that received a diet containing 3 and 6 Nano-Cu mg kg-1 diet, compared to the performance of fish that received diets containing 3 and 6 Bulk-Cu mg kg-1 diet and the control diet. Villi height, villi width, absorption area of villous (AAV), and mucosal to serosal amplification ratio (MSR) values demonstrated a substantial increase (P ≤ 0.05) in tilapia fed 3 and 6 mg kg-1 Nano-Cu compared to the values observed in fish fed the control and Bulk-Cu supplemented diets. Fish fed Bulk-Cu recorded the highest (P ≤ 0.05) hemoglobin concentration in those fed 6 mg kg-1 compared to 3 mg kg-1. Hematocrit value considerably improved (P ≤ 0.05) by supplementation of Cu, whereas the highest significant value demonstrated in fish provided 6 mg/kg-1 Nano-Cu. A fish-fed diet containing 3 mg kg-1 Nano-Cu revealed the best (P ≤ 0.05) values of plasma albumin, total protein, and globulins. Plasma HDL-C highest concentrations (P ≤ 0.05) were reported in fish fed diet supplemented with 6 mg/kg-1 either Bulk or Nano Cu, whereas values of plasma TG and VLDL-C declined as Cu supplementation level increased either from Bulk or Nano source. Also, the best (P ≤ 0.05) values of CAT and GPX were seen in fish given diet supplemented with 6 Nano-Cu mg/kg-1. Fillets of fish-fed Nano-Cu-supplemented diets showed a marked decline (P ≤ 0.05) in moisture and fat contents, while crude protein, ash, and Cu contents considerably increased in the fillet by dietary supplementation of Nano-Cu at both levels 3 and 6 mg kg-1. In conclusion, the supplemental diets with 3 or 6 Nano-Cu mg/kg-1 enhanced growth, feed utilization, body indices, fillet nutrient composition, hematological assay, plasma metabolites, immune, antioxidant activities, and intestinal morphometry of Nile tilapia.

Nano Zinc Versus Bulk Zinc Form as Dietary Supplied: Effects on Growth, Intestinal Enzymes and Topography, and Hemato-biochemical and Oxidative Stress Biomarker in Nile Tilapia (Oreochromis niloticus Linnaeus, 1758).

Five isonitrogenous diets were formulated to comprise two forms of zinc (Zn): convention zinc oxide named Bulk-ZnO or zinc oxide nanoparticles (Nano-ZnO) supplemented at two levels 30 and 60 mg kg-1 compared to the control diet. Nile tilapia, Oreochromis niloticus, fingerlings (5.02-5.05 g) were fed tested diets two times a day for 84 days. The results displayed that the best growth and digestive enzyme activity (P < 0.05) were noticed in fish fed 60 mg kg-1 Nano-ZnO. Moreover, significant (P < 0.05) improvement in intestinal topography was observed in 60 mg kg-1 Nano-ZnO group versus other treatments. Furthermore, fish fed 30 mg kg-1 Nano-ZnO recorded the best values of hematological indices (P < 0.05). The alanine and aspartate aminotransferase (ALT and AST) values were lower, while total serum protein, albumin, and globulin contents were clearly higher in fish fed diet that contained 30 mg kg-1 Nano-ZnO versus other groups. The significant highest values of oxidative enzyme activity escorted with lower malondialdehyde value recorded of fish fed diet supplemented with 60 mg kg-1 Nano-ZnO. The results indicated that inclusion of Nano-ZnO at 60 mg kg-1 was the recommended source to enhance growth, feed utilization, amylase and lipase enzymes activity, intestinal morphology, hemato-biochemical, and oxidative response biomarkers of Nile tilapia compared with Bulk-ZnO in commercial tilapia feeds.

A mechanistic model of nutritional control of protein synthesis in animal tissues.

Regulation of mRNA translation has been held responsible for effects of diet, age, alcohol, hormones, hibernation, disease and hypoxia on protein synthesis in animal tissues. Dietary effects are due to concentrations of amino acids and insulin in circulation that affect activities of two key translational regulators, eukaryotic initiation factor 2 (F2) and eukaryotic initiation factor 4E binding protein 1 (Bp). To construct a platform for prediction of global protein synthesis to nutritional stimuli, a dynamic, mechanistic model of translational control in whole tissues was developed. The model was composed of a set of differential equations which describe the dynamics of 11 state variables: tRNA and acyl-tRNA for leucine (Leu), limiting (Laa) and other amino acids (Oaa), inactivated F2 with GDP (F2d), activated F2 with GTP (F2t), F4e, Bp and its complex with F4e (4eBp), available mRNA start codons (AUG), and active ribosomes (Arib). Material was assumed to flow from one variable to another according to mass-action kinetics or Michaelis-Menten form. Uncharged tRNA inhibit GTP exchange on eIF2, and free amino acids and insulin inhibit reversible sequestration of F4e by Bp. Initial conditions and parameters were set for a skeletal muscle fractional synthesis rate of 10%/d and ribosome transit time of 80s. Between amino acid concentrations of 500 and 4000x10(3)nM, protein synthesis increased from 0.9 to 11.7%/d at 0microU/mL insulin, and from 5.0 to 12.8%/d at 30microU/mL insulin. Predicted responses to graded levels of a deficient amino acid were asymptotic. A single parameter accomodated differences between tissues in insulin sensitivity. Seven parameters must be changed to simulate initiation and elongation rates in more active tissues such as liver, or in tissues of older mature animals. An increase in uncharged tRNA during insulin stimulation highlighted the physiological importance of coordinated regulation of amino acid supply by insulin. In conclusion, the regulation of F4e release from Bp by Ins and Leu, and of F2d recycling by uncharged tRNA can be tied together to describe a wide range of FSR values across tissues and physiological states.