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Nutraceuticals have received increased attention in sustainable aquaculture. Consequently, the present study aimed to evaluate the dietary effects of Mucuna pruriens (MP) seed extract on growth performance, immune status, hepatic function, biochemical profiles, gonadal histology, and expression of immune-related genes in mono-sex Nile tilapia (Oreochromis niloticus). Fish were allocated into four groups and received MP at rates of 0 (control), 2, 4, and 6 g/kg diet, respectively, for 90 days. The results revealed that MP significantly (P<0.05) modulated growth performance (specific growth rate, final length, and length gain rate, body mass gain, and feed conversion ratio), lysozyme activity, and liver enzymes (AST, ALT). However, a non-significant effect on nitric oxide (NO) or immunoglobulin M (IgM) levels was detected, whereas the dietary inclusion of MP had a hypoglycemic effect. In terms of plasma globulin, albumin, globulin/albumin ratio, and cortisol, the MP receiving groups showed insignificant difference (P<0.05) when compared to controls, except for the 2 g MP-supplemented group. The lower inclusion concentration of MP (2 g/kg diet) demonstrated the best result (P < 0.05) for gonadosomatic index (GSI) and plasma testosterone level that was consistent with the histological findings reflecting an improvement in the testicular development compared with the control group. Expressions of complement component (C5) and interleukin 1-ß (IL-1ß) genes were significantly up-regulated in MP receiving groups. In conclusion, M. pruriens can be used as a safe natural economic feed additive and a low inclusion level of 2 g/kg diet is recommended to improve growth, enhance immunity, maintain liver functioning, improve testicular development, and to modulate immune-related genes in the mono-sex O. niloticus.
Assuntos
Ciclídeos , Doenças dos Peixes , Mucuna , Albuminas , Ração Animal/análise , Animais , Dieta/veterinária , Suplementos Nutricionais/análise , Extratos Vegetais , SementesRESUMO
Lead toxicity is a common occupational and environmental health hazard that exerts many toxic effects on animals and humans, including immunotoxicity. Curcumin (CUR) and cinnamon (CIN) are common medicinal herbs with immunostimulatory and antioxidant properties. Therefore, this study investigated the protective effect of curcumin and cinnamon against lead acetate (LA)-induced splenotoxicity in rats via hemato-biochemical, immunological, oxidative stress marker, CYP-2E1 expression, histological, and immunohistological evaluations. Four groups of seven rats each were used: the control group received corn oil as a vehicle; the lead acetate group received (100 mg/kg), the CUR + LA group received curcumin (400 mg/kg) plus lead acetate, and the CIN + LA group received cinnamon (200 mg/kg) plus lead acetate orally for 1 month. LA exposure induced macrocytic hypochromic anemia, leukocytosis, neutrophilia, monocytosis, and lymphopenia. Additionally, significant elevations in serum iron, ferritin levels, and transferrin saturation percentage with significant decline of total and unsaturated iron binding capacities (TIBC and UIBC), transferrin, and immunoglobulin G and M levels were recorded. In addition, lead acetate significantly upregulated splenic CYP-2E1 expression, that was evident by significant depletion of reduced glutathione (GSH) activity and elevation of malondihyde (MDA), nitric oxide (NO), and protein carbonyl (PC) concentrations in the spleen. Histologically, hyperplasia of lymphoid follicles, hemosiderin deposition, and disturbance of CD3 and CD68 immuno-expressions were evident in the spleen from the lead acetate group. However, curcumin and cinnamon administration restored the hemato-biochemical, immunological, and oxidative stress parameters as well as histological and immunohistological pictures toward normalcy. In conclusion, curcumin and cinnamon can partially ameliorate LA-induced oxidative damage in the spleen, possibly through their antioxidant, immunomodulatory, and gene-regulating activities.
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Monosodium glutamate (MSG), common flavor enhancer and feed additive, causes male reproductive dysfunction. However, Roselle tea, popular Hibiscus sabdariffa (HS) beverage, has a controversial effectiveness on male fertility. Therefore, the current study aimed to investigate either the adverse effect of aqueous HS extract (HSE) on the testicle or its potential ameliorative role including some stress markers, biochemical and immunohistochemical expressions in rats subjected to MSG. Here, the animals were divided into four groups that were given distilled water, HSE, MSG, and HSE + MSG respectively via gavage. After 6 weeks from the beginning of experiment, blood samples were collected for hormonal analysis. Additionally, testicular specimens were excised and processed for oxidative/antioxidant parameters determination, histological examination, and immunohistochemical evaluation of Bax and PCNA positive spermatogenic cells. Preliminary phytochemical analyses as well as antioxidant capacity of the HSE were tested. Our results revealed a strong inhibitory activity of the HSE phytochemical constituents against DPPH radical. MSG group revealed a significant decrease of testosterone, LH, FSH, and antioxidant parameters with elevated MDA compared with control and HSE groups. Additionally, an alteration of the testicular histo-architecture was observed among MSG group along with increased Bax and decreased PCNA positive cells. Meanwhile, the HSE showed a potent protective effect against testicular damage as well as oxidative stress induced by MSG. On the whole, our findings provide evidence that HSE can ameliorate MSG-induced testicular toxicity via induction of cell proliferation along with reduction of oxidative stress and cellular apoptosis in adult rat that attributed to the antioxidant and antiapoptotic effects of its phytochemical constituents.
Assuntos
Antioxidantes , Hibiscus , Animais , Apoptose , Masculino , Estresse Oxidativo , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Ratos , Glutamato de Sódio/toxicidade , TestículoRESUMO
Diazinon (DZN), a common organophosphorus insecticide (OPI), has hazardous effect to human and animals with its ubiquitous use. Considering the implication of reactive oxygen species (ROS) in the OPIs toxicity, the present study was aimed to evaluate the ameliorative properties of basil (BO) and sesame (SO) seed oils against the toxic effect of DZN. Forty adult male albino rats were divided into four experimental groups (n = 10 rats/group); control, DZN (10 mg/kg b.w/day), DZN + BO (5 ml/kg b.w/day), and DZN + SO (8 ml/kg b.w/day) groups, treated for a period of 4 weeks. DZN-exposed animals showed significant elevation in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (Cr) with a significant decline in testosterone level compared with control. On the other hand, DZN + BO and DZN + SO groups revealed significant decreases in ALT, AST, BUN, and Cr with a significant increase in testosterone level when compared with DZN-exposed animals. Oxidative/antioxidant indices revealed significant increases of malondialdehyde (MDA) levels along with significant decreases of superoxide dismutase (SOD), glutathione peroxidase (Gpx), and catalase (CAT) activities among DZN-treated rats compared with control. Distinctly lower levels of MDA and increased activities of SOD, Gpx, and CAT were evident in both DZN + BO and DZN + SO groups when compared with DZN-exposed animals. Inflammatory and immuno-modulatory markers assessment showed a significant increase in TNF-α with a significant decline in IL-10 level in DZN group; meanwhile, both DZN + BO and DZN + SO groups revealed significant declines in levels of TNF-α with significant increases in IL-10. Corresponds immunohistochemistry, the total scores (TS) of TNF-α immunostainings in hepatorenal, testicular, and epididymal tissues of control, DZN + BO and DZN + SO groups were significantly lower than those values of DZN group. Additionally, the examined tissues of DZN + BO group revealed significant lower TS of TNF-α immunostaining compared with DZN + SO group. The overall data suggested that both BO and SO can be efficiently used as preventive herbal compounds against DZN-induced oxidative stress with special reference to their possible antioxidant, anti-inflammatory, and free radical activities. However, BO has more potent protective effect against DZN-induced tissue injury at both immunohistochemical and molecular levels.
Assuntos
Diazinon , Ocimum basilicum , Sesamum , Animais , Antioxidantes/metabolismo , Diazinon/toxicidade , Fígado/metabolismo , Estresse Oxidativo , Óleos de Plantas/metabolismo , Ratos , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Background: The heart ventricles have thicker walls than atrium as they pump blood through blood vessels into all body organs. Aim: This study aimed to describe the histological changes of the heart ventricles in Egyptian bovine (Bos aegyptiacus) with special reference to Purkinje fibers. Methods: A total of 10 male Egyptian bovines of 1-10 years old were divided into three groups according to age; immature, mature, and adult animals. Results: The histological sections from all examined animals' groups revealed three different layers of the wall of both right and left ventricles; endocardium, myocardium, and epicardium. The endocardium was lined with endothelium and filled with fibrous connective tissue. The endocardium of adult bovine was the thickest. Purkinje fibers appeared of pale cytoplasm with few myofibrils. They were present in the deep layer of the endocardium and in the myocardium. The size of Purkinje fibers and the amount of their myofibrils appeared to be increased with advanced age. Bundles of cardiac muscles were the main constituent of the myocardium. The myocardial bundles were separated by fine connective tissue in immature animals that showed an increased amount in the adult animals. The hypereosinophilic cardiac muscle cells were observed in the ventricles of both mature and adult animals suggesting hypercontraction during rigor mortis. An external layer of the ventricles was the epicardium which consisted of connective tissue and covered with mesothelium. Conclusion: Overall, this study revealed histological changes in the wall of the ventricle and Purkinje fibers of Egyptian bovines (B. aegyptiacus) in relation to age. Additionally, the hypereosinophilia of the cardiac muscle cells was recorded in the ventricles of mature and adult bovines.
Assuntos
Bovinos/anatomia & histologia , Endocárdio/anatomia & histologia , Ventrículos do Coração/anatomia & histologia , Pericárdio/anatomia & histologia , Ramos Subendocárdicos/anatomia & histologia , Envelhecimento , Animais , Egito , MasculinoRESUMO
The retina possesses few types of neurons so; it is considered an excellent model for understanding the neural mechanisms underlying basic neural information processing in the brain. Glutamate is the major excitatory neurotransmitter in the vertebrate central nervous system and retina. The present study was carried out to characterize the expression pattern of vesicular glutamate transporter 2 (Vglut2) and 3 (Vglut3) and glutamate receptor 1 (GluR1) and 2 (GluR2) mRNAs in the retina of adult laughing dove (Streptopelia senegalensis) by RT-PCR and in situ hybridization histochemistry. The cerebellum of adult laughing dove was used as a positive control in this study. Vglut2 mRNA was highly expressed only in the granular layer of the cerebellum while Vglut3 mRNA was weakly expressed only in the Purkinje cells layer. In the retina, Vglut2 mRNA was highly expressed in the ganglion cell layer and moderately expressed in the inner nuclear layer while Vglut3 mRNA was moderately expressed only in the inner nuclear layer. GluR1 mRNA was intensely expressed in the Purkinje cells layer while GluR2 mRNA signals were highly detectable in both granular and Purkinje cells layers of the cerebellum. In the retina, moderate expression of GluR1 and intense expression of GluR2 was found in both ganglion cell layer and the internal half of inner nuclear layer mostly amacrine cells. These results suggest that some retinal neuronal cells in the adult laughing dove are glutamatergic. Therefore, GluR1 and 2 are suggested as useful markers for glutamatergic retinal neuronal cells in the adult laughing doves.
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Sistemas de Transporte de Aminoácidos Acídicos/metabolismo , RNA Mensageiro/metabolismo , Receptores de Glutamato/metabolismo , Retina/metabolismo , Proteína Vesicular 2 de Transporte de Glutamato/metabolismo , Sistemas de Transporte de Aminoácidos Acídicos/genética , Animais , Columbidae , Hibridização In Situ , RNA Mensageiro/genética , Receptores de Glutamato/genética , Proteína Vesicular 2 de Transporte de Glutamato/genéticaRESUMO
Cisplatin (CP) is one of the most active medications in cancer treatment and has some adverse effects such as hepatotoxicity and nephrotoxicity. The present research was planned to determine the protective effects of L-carnitine (LC) against CP-induced hepato-renal oxidative stress in rats, via investigating of some serum biochemical and tissue oxidative/antioxidant parameters, histological alterations, and immunohistochemical expressions of two different intermediate filaments (IFs) proteins; vimentin (VIM) and cytokeratin 18 (CK18). Twenty-eight rats were divided into four groups (7 rats each). Groups I and II were orally administered saline and LC (100 mg/kg body weight), respectively, once daily for 30 consecutive days. Group III received saline orally once daily and a single dose of CP on the 27th day of the experiment [7.5 mg/kg, intraperitoneal (IP)]. Group IV received both LC and CP. Injection of CP significantly (P ≤ 0.05) increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities and creatinine and urea levels, while serum total protein, albumin, and globulin concentrations significantly (P ≤ 0.05) decreased. In addition, CP induced a dramatic increase in the Malondialdehyde (MDA) level along with a substantial decrease in reduced glutathione (GSH) and catalase (CAT) in the hepato-renal tissues. Histologically, both liver and kidney of the CP treated group revealed marked degenerative changes. Moreover, overexpression of both VIM and CK18 in hepato-renal tissues were noted after CP injection. On the other hand, the administration of LC in the CP injected group (Group IV) restored the biochemical parameters, histological, and immunohistochemical pictures toward the normalcy. In conclusion, LC may be supplemented for chemotherapy with CP to ameliorate its oxidative stress and restore the normal organization of IFs, especially VIM and CK18 within the CP intoxicated hepato-renal cells.
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The moderator band (MB) is a common fibromuscular anatomical structure for the right ventricle of most animals. The histomorphometric and immunohistochemical analysis of the MBs of Egyptian Baladi cattle in relation to age was the aim of this study. Eighteen clinically healthy animals of both sexes were used for this study. The animals were divided into three groups depending on age, group I (N = 4, <1 year), group II (N = 8, 1-2 years) and group III (N = 6, 4-8 years). Cross sections of the MBs from all groups were stained with H&E, Masson's trichrome and anti-connexin43 (Cx43) antibody for histological and immunohistochemical examinations. Also, measurements for the thickness of the endocardium of the MB as well as, the wall of its muscular artery were conducted. Bundles of Purkinje fibres (PFs) were identified peripherally in the endocardial layer and among the myocardial fibres in the core of each MB. The infiltration of endocardial adipocytes was the characteristic for MBs of old animals. All morphometric data showed a significant increase with the advancement of age. Immunohistochemical findings revealed the localization and distribution of Cx43 in the PFs and intercalated discs of all examined MBs. However, variation of Cx43 immunoreactivity was found among the groups depending on the age. On the basis of this study, this conclusion of different histomorphometry and Cx43 expression of the MBs in relation to age was drawn. These interesting findings provide further insight into age-related physiological and pathological heart conditions.
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Envelhecimento/fisiologia , Bovinos/anatomia & histologia , Coração/anatomia & histologia , Coração/fisiologia , Animais , Bovinos/fisiologia , Feminino , MasculinoRESUMO
This study was conducted to demonstrate the effect of castration on the structure of vesicular glands of the Egyptian Nubian (Zaraibi) goat. Vesicular glands of castrated (n=4) and intact (n=6) goat were used for histological and immunohistochemical evaluations. In this study, we report the difference in cell specific expression of androgen receptor (AR) and cyclooxygenase-2 (COX-2) in the vesicular glands of castrated and intact goats. In both castrated and intact goats, the present study revealed no immunopositive cells for AR or COX-2 in the fibromuscular stroma meanwhile, AR and COX-2 containing immunoreactive cells were restricted only to the epithelium of the secretory acini of the vesicular gland. Such finding suggests androgen and COX-2 as important regulators for the growth and secretory activity of epithelial cells in the vesicular gland of goats. Overall, the vesicular gland of castrated goats showed significantly (P<0.05) lower AR and COX-2 immuno-expression than intact goats indicating that both AR and COX-2 are androgen dependent.
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Ciclo-Oxigenase 2/metabolismo , Cabras/metabolismo , Receptores Androgênicos/metabolismo , Glândulas Seminais/enzimologia , Animais , Masculino , Orquiectomia , Glândulas Seminais/citologiaRESUMO
Uteri of mature Egyptian buffalo cows (5-10 years old) were collected at follicular (n=12) and luteal (n=16) phases of estrous cycle to investigate the expression of calbindin-D9k (CaPB-9k) and vitamin D receptor (VDR). This study was done using avidin-biotin immunohistochemistry method. In addition, blood levels of calcium (Ca), vitamin D3 (Vit D), estrogen (E2) and progesterone (P4) were measured. The immunohistochemical findings restricted the expressions of CaBP-9k and VDR to the luminal and glandular epithelia of the endometrium implicating the importance of CaBP-9K and VDR in the function of endometrial epithelium, especially the glandular one, in order to prepare a receptive uterus. On the other hand, the myometrium did not express CaBP-9k or VDR that denies the potential role of CaBP-9k and VDR in the uterine contractility during the estrous cycle of Egyptian buffalo. All of Ca, Vit D, and P4 blood levels significantly (P<0.05) increased during luteal phase however, blood level of E2 significantly (P<0.05) increased during follicular phase. The expressions of CaBP-9k and VDR in the uterus of Egyptian buffalo were significantly (P<0.05) higher during luteal (P4 dominant) phase than during the follicular (E2 dominant) phase indicating that P4 up-regulates the expressions of CaBP-9k and VDR. In view of these observations, this study represents the first characterization of CaBP-9K and VDR expression in the uterus of Egyptian buffalo and suggests the pivotal role of CaBP-9k and VDR in the uterine receptivity. Furthermore, it demonstrates the regulatory role of P4 for expressions of CaBP-9k and VDR in buffalo uterus.
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Búfalos/fisiologia , Receptores de Calcitriol/metabolismo , Proteína G de Ligação ao Cálcio S100/metabolismo , Útero/metabolismo , Animais , Ciclo Estral , Feminino , Fase Folicular , Fase Luteal , Útero/citologiaAssuntos
Hibiscus , Animais , Antioxidantes , Masculino , Compostos Fitoquímicos , Extratos Vegetais , Ratos , Glutamato de SódioRESUMO
Ten adult, cyclic female camels (Camelus dromedarius) were used to describe the distribution of androgen (AR) and progesterone (PR) receptors in the uterus using immunohistochemistry. Both AR and PR were distributed throughout the different compartments of the uterus with nuclear staining for AR and PR seen in the cells of epithelia (luminal and glandular), stroma and myometrial smooth muscles. AR immunostaining was not uniform in distribution and intensity; the surface epithelium and the glandular epithelium in the adluminal region of the endometrium showed lower AR immunoreactivity than other compartments of the uterus. PR immunostaining showed uniformity in both distribution and intensity strong PR immunostaining intensity in almost all cells of the different uterine compartments. The intensity and distribution of PR immunostaining in epithelia of lumen and glands in the adluminal regions of endometrium was higher (P<0.05) than that of AR. In conclusion, immunohistochemical localization of AR and PR in the uterus of the cyclic dromedary camel indicates the important roles of androgen and progesterone in controlling the uterine activity during the follicular phase.
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Camelus/metabolismo , Receptores Androgênicos/metabolismo , Receptores de Progesterona/metabolismo , Útero/metabolismo , Animais , Feminino , Imuno-HistoquímicaRESUMO
Thioacetamide (TAA) is one of the common fungicidal agents that induce liver injury varying from inflammation, necrosis, and fibrosis to cirrhosis. Many recent studies reported the beneficial effect of probiotics and silymarin on hepatotoxicity regardless the causative agents. Therefore, the present study aimed to evaluate the ameliorative role of probiotics and/or silymarin on TAA induced hepatotoxicity in rats via histological, and immunohistochemical methods. Twenty five male albino rats were used for this experiment and were divided into five groups (n=5 rats/group); group I acts as negative control, group II was orally administrated distilled water for six weeks, then injected with TAA (200 mg/kg b.wt./ 5 ml physiological saline/ I.P.) twice a week for another six weeks, group III was treated with probiotics at a dose of 135 mg/ kg b.wt. orally in drinking water daily for six weeks, then injected with TAA (dosage of group II), twice weekly for another six weeks, group IV was treated with silymarin at a dose of 200 mg/ kg b.wt orally 4 times per week for six weeks, then injected with TAA (dosage of group II), twice weekly for another six weeks and group V was treated with combination of both probiotics and silymarin, at the same dosage in groups III and IV respectively then injected with TAA (dosage of group II), twice weekly for another six weeks. Histologically, TAA induced hepatocytes degeneration, inflammatory cells infiltration, and pseudolobular parenchyma as well as, high apoptosis and low proliferation rates that were proved by immunohistochemical staining for caspase 3 and ki-67 respectively. Probiotics and/or silymarin improved the histological feature of hepatocytes, reduced apoptosis and stimulated proliferation. Based on these results, we concluded that the use of probiotics and silymarin combination ameliorates the hepatotoxic effect of TAA in rats more than the use of probiotics or silymarin alone.
La tioacetamida (TAA) es uno de los agentes fungicidas más comunes que inducen lesiones hepáticas que varían desde inflamación, necrosis y fibrosis hasta cirrosis. Muchos estudios recientes informaron el efecto beneficioso de los probióticos y la silimarina sobre la hepatotoxicidad independientemente de los agentes causantes. Por lo tanto, el presente estudio tuvo como objetivo evaluar el papel paliativo de los probióticos y / o silimarina en la hepatotoxicidad inducida por TAA en ratas a través de métodos histológicos e inmunohistoquímicos. Para este experimento se usaron veinticinco ratas albinas y se dividieron en cinco grupos (n = 5 ratas / grupo); el grupo I se usó como control negativo; en el grupo II se administró por vía oral agua destilada durante seis semanas y luego se inyectó TAA (200 mg / kg b.wt./ 5 ml solución salina fisiológica / IP) dos veces por semana durante otras seis semanas; el grupo III se trató con probióticos, dosis diaria de 135 mg / kg b.wt. por vía oral en agua potable, durante seis semanas y luego fue inyectado con TAA (dosis del grupo II), dos veces por semana durante otras seis semanas; el grupo IV se trató con silimarina, con una dosis de 200 mg / kg b.wt por vía oral 4 veces por semana durante seis semanas, luego se inyectó TAA (dosificación del grupo II), dos veces por semana durante otras seis semanas; y el grupo V, se trató con una combinación de ambos probióticos y silimarina con la misma dosis que en los grupos III y IV, respectivamente, luego fueron inyectados con TAA (dosificación del grupo II), dos veces por semana durante otras seis semanas. Histológicamente, la TAA indujo la degeneración de los hepatocitos, la infiltración de células inflamatorias y el parénquima pseudolobular, así como también una apoptosis alta y tasas de proliferación bajas que se probaron mediante tinción inmunohistoquímica para caspasa 3 y ki-67, respectivamente. Los probióticos y / o la silimarina mejoraron la característica histológica de los hepatocitos, redujeron la apoptosis y estimularon la proliferación. En base a estos resultados, concluimos que el uso de la combinación de probióticos y silimarina mejora el efecto hepatotóxico del TAA en ratas más que el uso de probióticos o silimarina individualmente.
Assuntos
Animais , Masculino , Ratos , Silimarina/administração & dosagem , Tioacetamida/toxicidade , Probióticos/administração & dosagem , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Imuno-Histoquímica , Doença Hepática Induzida por Substâncias e Drogas/patologia , Fígado/efeitos dos fármacosRESUMO
Fourteen vomeronasal organs (VNOs) of adult males one-humped camels (Camelus dromedarius) and Egyptian water buffaloes (Bubalus bubalis) (n=7/each) were examined immunohistochemically with neuronal markers; synaptophysin (SYP) and glial fibrillary acidic protein (GFAP) to clarify the distribution of the vomeronasal (VN) receptor cells and nerve fibers, in addition to elucidate the existence of non-neuronal elements via S-100 and endothelial nitric oxide synthase (eNOS). In both animals, the VNO was lined medially with VN sensory (olfactory) epithelium and non-sensory (respiratory) epithelium laterally. Immunohistochemically, both animals showed SYP immunolabeling only in the receptor cells of VN sensory epithelium while GFAP labeled the ensheathing cells of the nerve fibers of VNOs. Both S-100 and eNOS labeled non-neuronal elements of the VNO; the supporting cells of sensory epithelium and the VN glands. In view of these observations, we postulate that the VNOs of both animals contain various cells populations that express several neuronal and non-neuronal markers. As well as, SYP and GFAP are suggested as markers for receptor cells and ensheathing cells of nerves of the VNOs respectively. However, no clear differences can be detected in the expressions of neuronal and non-neuronal markers in VNOs of camel and buffalo since they are ruminant species.
En este estudio fueron examinados 14 órganos vomeronasales (OVN) de machos adultos de camellos de una joroba (Camelus dromedarius) y búfalos egipcios de agua (Bubalus bubalis) (n = 7 / cada uno) por inmunohistoquímica con marcadores neuronales, sinaptofisina (SIP) y proteína ácida fibrilar glial (PAFG), para identificar la distribución vomeronasal (VN) del receptor de células y fibras nerviosas, además de dilucidar la existencia de elementos no neuronales a través de S-100 y óxido nítrico sintasa endotelial (ONSe). En ambos animales, el OVN se encuentra alineado en sentido medial con el epitelio sensorial (olfato) y lateralmente con el epitelio no sensorial (respiratorio). En el estudio inmunohistoquímico, ambos animales mostraron marcadores inmunológicos solamente en las células receptoras del epitelio sensorial VN, mientras que la proteína ácida fibrilar glial marcaba las fibras nerviosas de OVN. Tanto el S-100 como la óxido nítrico sintasa endotelial, marcaron elementos no neuronales del OVN, las células de revestimiento del epitelio sensorial y las glándulas VN. En relación a estas observaciones, se postula que los OVN de ambos animales contienen células que expresan varios marcadores neuronales y no neuronales. SIP y la PAFG se sugieren como marcadores para células receptoras y las células gliales de nervios del OVN, respectivamente. Sin embargo, debido a que son especies de rumiantes, no existen diferencias claras que se puedan detectar en las expresiones de los marcadores neuronales y no neuronales en el OVN de camello y búfalo.