Exogenous melatonin-mediated modulation of arsenic tolerance with improved accretion of secondary metabolite production, activating antioxidant capacity and improved chloroplast ultrastructure in rosemary herb.

Arsenic (As) recognized as a group I human carcinogen additionally poses a threat to plants which limit growth, metabolic activity, and productivity. Melatonin (MEL) is a naturally occurring compound in plants that have been recognized to mediate numerous morphological, physiological and molecular processes. Conversely, the role of MEL in inducing As-tolerance remains inexpressible and the plausible mechanisms in inducing As tolerance have remained largely unknown. The present investigation was designed to understand the protective role of MEL concentrations in rosemary herbs cultivated under As contamination. Arsenic evoked a deleterious decline on herb productivity, photosynthetic pigment, ion concentration, water status, ascorbic acid, essential oil (EO) yield and induced malformation of the chloroplast. Alternatively, increased organic osmolytes, oxidative impairment criteria, additionally antioxidant enzymes, phenol, flavonoid, anthocyanin, and EO%. Exogenous application of MEL with or without As, considerably increased growth, photosynthetic pigment, ion concentration, organic osmolytes as well as EO yield regarding polluted or non-polluted treatment respectively. Moreover, MEL treatment stabilized the cell membrane integrity, suppressed oxidative impairment criteria, and enhanced antioxidant capacity, additionally upregulation antioxidant enzymes. Plant treated with As showed a significant increase in As contamination and a bioconcentration factor in both root and shoot system. MEL supplementation under normal or As concentration, reduced As accumulation and bioconcentration factors, in either shoot or root systems. Additionally As decrease transfer factor, however, supplementation of MEL further decreased it. Application of 50 µM MEL might help the herbs to withstand As stress by strengthening their antioxidant machinery and osmoregulation capacity.

Potential Role of Biochar and Silicon in Improving Physio-Biochemical and Yield Characteristics of Borage Plants under Different Irrigation Regimes.

Silicon (Si) and biochar (Bc) are key signaling conditioners that improve plant metabolic processes and promote drought tolerance. However, the specific role of their integrative application under water restrictions on economical plants is not yet well understood. Two field experiments throughout 2018/2019 and 2019/2020 were conducted to examine the physio-biochemical modifications and yield attributes of borage plants mediated by Bc (9.52 tons ha-1) and/or Si (300 mg L-1) under different irrigation regimes (100, 75, and 50% of crop evapotranspiration). Catalase (CAT) and peroxidase (POD) activity; relative water content, water, and osmotic potential; leaf area per plant and yield attributes; and chlorophyll (Chl) content, Chla/chlorophyllidea (Chlida), and Chlb/Chlidb were considerably reduced within the drought condition. On the other hand, oxidative biomarkers, as well as organic and antioxidant solutes, were increased under drought, associated with membrane dysfunction, superoxide dismutase (SOD) activation, and osmotic adjustment (OA) capacity as well as a hyperaccumulation of porphyrin intermediates. Supplementation of Bc and Si lessens the detrimental impacts of drought on several plant metabolic processes associated with increasing leaf area and yield attributes. Their application under normal or drought conditions significantly elicited the accumulation of organic and antioxidant solutes as well as the activation of antioxidant enzymes, followed by lessening the formation of free radical oxygen and mitigating oxidative injuries. Moreover, their application maintained water status and OA capacity. Si and/or Bc treatment reduced protoporphyrin, magnesium-protoporphyrin, and protochlorophyllide while increasing Chla and Chlb assimilation and boosting the ratio of Chla/Chlida and Chlb/Chlidb, resulting in a rise in leaf area per plant and yield components following these modifications. These findings highlight the significance of Si and/or Bc as (a) stress-signaling molecule(s) in regulating defensive systems in drought-affected borage plants by boosting antioxidant aptitude, regulating water status, and accelerating chlorophyll assimilation, thus leading to increasing leaf area and productivity.

Sustainable Biochar and/or Melatonin Improve Salinity Tolerance in Borage Plants by Modulating Osmotic Adjustment, Antioxidants, and Ion Homeostasis.

Salinity is persistently a decisive feature confining agricultural sustainability and food security in arid and semi-arid regions. Biochar (Bi) has been advocated as a means of lessening climate changes by sequestering carbon, concurrently supplying energy and rising crop productivity under normal or stressful conditions. Melatonin (Mt) has been shown to mediate numerous biochemical pathways and play important roles in mitigating multi-stress factors. However, their integrated roles in mitigating salt toxicity remain largely inexpressible. A completely randomized design was conducted to realize the remediation potential of Bi and/or Mt in attenuation salinity injury on borage plants by evaluating its effects on growth, water status, osmotic adjustment, antioxidant capacity, ions, and finally the yield. Salinity stress significantly decreased the plant growth and attributed yield when compared with non-salinized control plants. The depression effect of salinity on borage productivity was associated with the reduction in photosynthetic pigment and ascorbic acid (AsA) concentrations, potassium (K+) percentage, K+-translocation, and potassium/sodium ratio as well as catalase (CAT) activity. Additionally, borage plants' water status was disrupted by salinity through decreasing water content (WC), relative water content (RWC), and water retention capacity (WTC), as well as water potential (Ψw), osmotic potential (Ψs), and turgor potential (Ψp). Moreover, salinity stress evoked oxidative bursts via hyper-accumulation of hydrogen peroxide (H2O2) and malondialdehyde (MDA), as well as protein carbonyl, which is associated with membrane dysfunction. The oxidative burst was connected with the hyper-accumulation of sodium (Na+) and chloride (Cl-) in plant tissues, coupled with osmolytes' accumulation and accelerating plants' osmotic adjustment (OA) capacity. The addition of Bi and/or Mt had a positive effect in mitigating salinity on borage plants by reducing Cl-, Na+, and Na+-translocation, and oxidative biomarkers as well as Ψw, Ψs, and Ψp. Moreover, Bi and/or Mt addition to salt-affected plants increased plant growth and yield by improving plant water status and OA capacity associated with the activation of antioxidant capacity and osmolytes accumulation as well as increased photosynthetic pigments, K+, and K+/Na+ ratio. Considering these observations, Bi and/or Mt can be used as a promising approach for enhancing the productivity of salt-affected borage plants due to their roles in sustaining water relations, rising solutes synthesis, progressing OA, improving redox homeostasis, and antioxidant aptitude.

Acaricidal Efficacy of Jasmine and Lavender Essential Oil or Mustard Fixed Oil against Two-Spotted Spider Mite and Their Impact on Growth and Yield of Eggplants.

Eggplant is repeatedly attacked by numerous pests, particularly two-spotted spider mite (TSSM), which considerably decline plant productivity. Synthetic acaricides are frequently applied for controlling TSSM, resulting in environmental pollution. The utilization of rational novel substances which repel or prevent TSSM establishment represents a sustainable eco-friendly to reduce the utilization of agrochemicals. A greenhouse investigation was done for assessing the bio-acaricidal activity of mustard (Brassica juncea L.) fixed oil (MFO), jasmine (Jasminum grandiflorum L.) essential oil (JEO), or lavender (Lavandula angustifolia L.) essential oil (LEO), and their influences on eggplant growth and productivity. The results demonstrated that JEO represents the most acaricidal properties against TSSM followed by MFO and/or LEO compared to control. Spraying with natural oils significantly improved eggplant growth, i.e., plant height, number of leaves, and branches/plant, in addition to the leaf area and relative leaf dry mass of the 3rd-5th upper leaves. The JEO had the strongest positive effect compared with other oils or control. Additionally, Natural oils application significantly increased photosynthetic pigment, chlorophyll a:b ratio, and nitrogen, phosphorus, potassium, ascorbic acid, and phenols. The application of oils increased yield and its quality. In this study, JEO (2.5 mL/l) is shown to be extremely promising for the progress of new eco-friendly acaricides, improving plant growth and increasing eggplant yield.

Zinc and Paclobutrazol Mediated Regulation of Growth, Upregulating Antioxidant Aptitude and Plant Productivity of Pea Plants under Salinity.

Soil salinity is the main obstacle to worldwide sustainable productivity and food security. Zinc sulfate (Zn) and paclobutrazol (PBZ) as a cost-effective agent, has multiple biochemical functions in plant productivity. Meanwhile, their synergistic effects on inducing salt tolerance are indecisive and not often reported. A pot experiment was done for evaluating the defensive function of Zn (100 mg/L) or PBZ (200 mg/L) on salt (0, 50, 100 mM NaCl) affected pea plant growth, photosynthetic pigment, ions, antioxidant capacity, and yield. Salinity stress significantly reduces all growth and yield attributes of pea plants relative to nonsalinized treatment. This reduction was accompanied by a decline in chlorophyll, nitrogen, phosphorus, and potassium (K+), the ratio between K+ and sodium (Na+), as well as reduced glutathione (GSH) and glutathione reductase (GR). Alternatively, salinity increased Na+, carotenoid (CAR), proline (PRO), ascorbic acid (AsA), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) over nonsalinized treatment. Foliar spraying with Zn and PBZ under normal condition increased plant growth, nitrogen, phosphorus, potassium, K+/Na+ ratio, CAR, PRO, AsA, GSH, APX, GR, and yield and its quality, meanwhile decreased Na+ over nonsprayed plants. Application of Zn and PBZ counteracted the harmful effects of salinity on pea plants, by upregulating the antioxidant system, ion homeostasis, and improving chlorophyll biosynthesis that induced plant growth and yield components. In conclusion, Zn plus PBZ application at 30 and 45 days from sowing offset the injuries of salinity on pea plant growth and yield by upregulating the antioxidant capacity and increasing photosynthetic pigments.