Treatment of Synthetic Ammonium Sulfate Wastewater by Mixed Culture of Chlorella pyrenoidosa and Enriched Nitrobacteria.

Ammonium sulfate wastewater can cause eutrophication and black odor of water body. Although ammonia nitrogen can be used as nutrient of microalgae, high ammonia nitrogen levels could inhibit the growth of microalgae. Nitrobacteria can transform ammonia nitrogen into nitrate nitrogen. In this study, mono Chlorella pyrenoidosa culture (mono-C.py), synchronous mixed culture (mixed-a), and asynchronous mixed culture (mixed-b) systems were examined for their ability to treat ammonium sulfate wastewater. Nitrogen removal rate of mixed-b at the end of culture (52.96%) was higher than that of the mono-C.py (46.37%) and the mixed-a (39.11%). Higher total suspended solid concentration (2.40 g/L), crude protein yield (0.76 g/L), and heating value yield (35.73 kJ/L) were obtained in mixed-b, meanwhile with excellent settlement performance (91.43 ± 0.51%). Mechanism analysis of settlement showed that the relative abundance of floc-forming-related bacteria Sphingopyxis and Acidovorax were increased generally, while nitrification/denitrifying members were decreased in mixed-b along with the culture proceeding.

Treatment and resource utilization of dairy liquid digestate by nitrification of biological aerated filter coupled with assimilation of Chlorella pyrenoidosa.

Ammonia inhibition is considered a key issue when using liquid digestate for microalgae cultivation. To study the effect of pretreatment with a biological aerated filter (BAF) on microalgae culture with dairy liquid digestate, nitrification characteristics of BAFs under different hydraulic retention time (HRT) and the growth characteristics of Chlorella pyrenoidosa in effluents of BAFs were investigated. Results showed that the BAFs can rapidly nitrify ammonia nitrogen and significantly improve the light transmittance of liquid digestate (the maximum promotion rate was ~260%), and the effect improved as the HRT increased. Pretreatment of liquid digestate with BAFs can eliminate ammonia inhibition for C. pyrenoidosa. Furthermore, lipid, crude protein, and higher heating value (HHV) output were also not affected by HRT. The similar removal of nitrate nitrogen in microalgae culture systems using effluents with 6-h and 12-h HRT (21.59% and 21.07%, respectively) were recorded. The results suggested that BAF coupled with microalgae culture is a novel option on the resource utilization of dairy liquid digestate.