RESUMO
In the title three-dimensional tetrazolate-based coordination polymer, poly[bis(µ(3)-cyanido-κ(3)N:C:C)[µ(5)-5-(pyridin-4-yl)tetrazolato-κ(5)N:N':N'':N''':N'''']tricopper(I)], [Cu(3)(C(6)H(4)N(5))(CN)(2)](n), there are two types of coordinated Cu(I) atoms. One type exhibits a tetrahedral environment and the other, residing on a twofold axis, adopts a trigonal coordination environment. The closest Cu···Cu distance is only 2.531 (2) Å, involving a bridging cyanide C atom. All four tetrazolate and the pyridine N atom of the 4-(pyridin-4-yl)-1H-tetrazolate anion are coordinated to these Cu(I) atoms and exhibit a µ(5)-bridging mode. The three-dimensional coordination network can be topologically simplified as a rarely observed (3,3,4,5)-connected network with the Schläfli symbol (4.6.8(4))(2).(4(2).6.8(7)).(6.8(2))(3).
RESUMO
The asymmetric unit of the title compound, C(6)H(5)N(5)·C(9)H(6)O(6), comprises a full mol-ecule each of neutral trimesic acid (tma) and zwitterionic 5-(pyridin-1-ium-3-yl)-5H-1,2,3,4-tetra-zol-5-ide (ptz). The components are linked into a two-dimensional layer by a combination of O-Hâ¯O, O-Hâ¯N, N-Hâ¯O and N-Hâ¯N hydrogen bonds parallel to the (10[Formula: see text]) plane. Layers comprising alternating rows of tma and ptz are linked into a three-dimensional network by C-Hâ¯O and π-π inter-actions between tma and tetra-zolate rings [centroid-centroid distance = 3.763â (2)â Å], and between pyridinium and tetra-zolate rings [centroid-centroid distance = 3.745â (2)â Å].
RESUMO
Podocyte apoptosis coincides with albuminuria onset and precedes podocytopenia in diabetic nephropathy. However, there is a lack of effective therapeutic drugs to protect podocytes from apoptosis. Here, we demonstrated that resveratrol relieved a series of indicators of diabetic nephropathy and attenuated apoptosis of podocytes in db/db diabetic model mice. In addition, resveratrol induced autophagy in both db/db mice and human podocytes. Furthermore, inhibition of autophagy by 3-methyladenine (3-MA) and autophagy gene 5 (Atg5) short hairpin RNA (shRNA) reversed the protective effects of resveratrol on podocytes. Finally, we found that resveratrol might regulate autophagy and apoptosis in db/db mice and podocytes through the suppression of microRNA-383-5p (miR-383-5p). Together, our results indicate that resveratrol effectively attenuates high glucose-induced apoptosis via the activation of autophagy in db/db mice and podocytes, which involves miR-383-5p. Thus, this study reveals a new possible strategy to treat diabetic nephropathy.
Assuntos
Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Nefropatias Diabéticas/tratamento farmacológico , Podócitos/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Estilbenos/farmacologia , Albuminúria/tratamento farmacológico , Albuminúria/genética , Animais , Nefropatias Diabéticas/genética , Modelos Animais de Doenças , Camundongos , Camundongos Endogâmicos C57BL , RNA Interferente Pequeno/genética , ResveratrolRESUMO
The title compound, [Ag2(C6H4N4)(N3)]n, was obtained under hydrothermal conditions at 433â K. The asymmetric unit of the orthorhombic space group (Pna21) consists of two Ag(+) cations, an anionic 5-(pyridin-4-yl)tetrazolide (4-ptz(-)) ligand and an anionic azide ligand. Both Ag(+) centres are coordinated by four N atoms, forming a distorted tetrahedral coordination environment. When all the component ions are viewed as 4-connected nodes, the whole three-dimensional network can be regarded topologically as a new kind of 4,4,4,4-connected net with the Schläfli symbol (4.8(5))(4(2).8(4))(4(3).8(3))2.