EFFECTS OF BEEF SEMEN AND BEEF EMBRYO STRATEGIES ON PROFITABILITY: Economics of using beef semen and beef in vitro produced embryo transfer in Jersey herds.

Dairy herds have adopted sexed semen (SS) and beef semen (BS) to control heifer inventory and increase calf sales revenue. Beef in vitro-produced embryo transfer (beef IVP-ET) may be an alternative to increase calf sales revenue. Besides, raising those Jersey beef crossbred and/or pure beef animals in a dairy system may be a new source of revenue. We aimed to evaluate breed strategies combining dairy conventional semen (CS), SS, BS, and beef IVP-ET on herd dynamics and profitability by marketing those animals with one-day-old or raising them to 180 kg. A Markov chain model was developed to maximize the profitability of Jersey herds by changing the number of dairy heifers sold at birth and the culling rate of 3rd and greater parity cows. The model presents inputs on the reproductive and productive performance of heifers and cows over time. The last year's data (year 10 - steady state) was used to calculate accrual operational cost and revenue per cow per year. We varied the breeding strategy by breeding order and parities, the embryo transfer cost ($85 or $170), the pure beef calf market price ($200 or $300), and by marketing Jersey-beef and pure beef animals with one-day-old or raising them to 180 kg. A total of 8 scenarios + default scenario were simulated. Overall, the proportion of SS use was 47.3 ± 0.6%. For the scenarios replacing all CS breedings with BS breedings, the proportion of CS and BS used was 52.3 ± 0.6. When beef IVP-ET was used, the percentage of BS and beef IVP-ET used was 22.4 ± 0.1% and 31.0 ± 0.1%, respectively. We observed that when we compared SS:BS with the default scenario, the production of purebred Jersey male calves was reduced by 83.5%, and profit/cow per year was increased from $113.5 to $203.3 with SS:BS. When a beef IVP-ET of $85 per transfer was used (scenarios 2 and 3), profit/cow per year was $145.5 and $176.2 for a pure-beef calf price of $200 and $300, respectively. In scenario 4, with a beef IVP-ET cost of $170, the lowest profit ($52.9 per cow per year) was found when marketing one-day-old pure-beef calves at $200. The highest profit was achieved for scenario raising the Jersey-beef crossbred animals to 180 kg ($232.9, scenario 6), followed by scenario 7 ($222.9, SS:BS:IVP-ET) with an embryo transfer cost of $85. Under the current market conditions, combining SS and BS in the reproductive program was a feasible economic opportunity for Jersey herds, yielding the highest net return. The adoption of beef IVP-ET in a reproductive program can potentially increase profit/cow per year, but its profitability will depend on the beef IVP-ET pregnancy cost, the pure-beef market price, calf performance, and the herd reproductive performance. In conclusion, raising the Jersey-beef crossbred calves may be a profitable strategy, and dairy producers need to evaluate the best option to invest in since it will take an extra risk to produce high-quality animals to the market.

Association of morbidity, mortality, and average daily gain with transfer of passive immunity in dairy-beef crossbred calves up to 60 d of life.

Adequate transfer of passive immunity (TPI) is a cornerstone for a proper health status of calves. In the literature, there is limited information on the prevalence of failure of TPI in dairy-beef crossbred calves and its impact on morbidity, mortality, and average daily gain (ADG) during the preweaning period. Therefore, this study aimed to evaluate the association between TPI with morbidity, mortality, and ADG in pre-weaned dairy-beef crossbred calves. A total of 1,055 newborn dairy-beef crossbred calves were enrolled upon arrival at a calf-raising facility in California from January to August 2021. Farm of origin, genetic breed group, sex, and body weight were recorded upon arrival. Blood was collected at 24 ± 1 h post-arrival to evaluate serum IgG concentration, serum total protein (TP), and packed cell volume. Morbidity (diarrhea and respiratory treatment records) and mortality were recorded daily until 60 d of life. Calves were grouped into 2 genetic breed groups: Holstein x Beef (Ho x Be, 49.6%) and Jersey × Beef crossbred calves (Je × Be, 50.4%). Descriptive statistics and Cox proportional hazard models were created to evaluate the association of TPI categories for serum IgG (TPI-IgG: poor: < 10.0 g/L, fair: 10.0 - 17.9 g/L, good: 18.0 - 24.9 g/L, and excellent: ≥ 25.0 g/L) and TP (TPI-TP: poor: < 5.1 g/dL, fair: 5.1 - 5.7 g/dL, good: 5.8 - 6.1 g/dL, and excellent: ≥ 6.2 g/dL), sex, and genetic breed group with morbidity and mortality. Additionally, a mixed linear regression was performed to evaluate the association of sex, genetic breed group, and TPI categories with ADG. Overall morbidity and mortality were 84.8% (n = 895) and 2.5% (n = 26). Calves classified as TPI-IgG excellent were associated with the lowest (43.2% less) hazard of being treated compared with TPI-IgG poor calves. For mortality, dairy-beef crossbred calves with TPI-IgG excellent were associated with a reduction of 82.0% in the hazard of dying compared with TPI-IgG poor. The TPI-IgG poor and TPI-IgG fair calves were associated with a decreased ADG of 101.0 and 98.8 g/d, respectively, in comparison with TPI-IgG good calves. Average daily gain of TPI-IgG good and TPI-IgG excellent calves were not different. In our study, dairy-beef crossbred calves enrolled may have endured challenging conditions that increased morbidity. This reinforces the importance of high IgG levels to decrease morbidity and mortality and maximize ADG in dairy-beef crossbred calves raising systems. Further research should evaluate the long-term effects of TPI categories on the health and performance of dairy-beef crossbred calves.

Physiological responses of Holstein calves and heifers carrying the SLICK1 allele to heat stress in California and Florida dairy farms.

Inheritance of the SLICK1 allele of the prolactin receptor gene improves thermotolerance of lactating Holstein cows under humid heat stress conditions. The aim of this study was to investigate whether pre- and postweaning Holstein heifers carrying the SLICK1 allele would show physiological responses indicative of higher tolerance to heat stress in high- and low-humidity climates. A total of 101 heifer calves of two age groups heterozygous for the SLICK1 allele and 103 wild-type half-siblings were evaluated during July 2020 in 3 dairy farms in central California and 2 in south Florida. Dry bulb temperature and relative humidity data were recorded during evaluation and used to calculate the temperature-humidity index (THI). Physiological measurements were obtained between 1600 and 1900 h in California, and 1200 and 1400 h in Florida and included rectal temperature, respiration rate, skin temperature, and sweating rate. Data were analyzed via Generalized Linear Mixed Models including the main effects of genotype, state, group, sire, farm within state, and interactions, with THI included as a covariate. The correlations between THI and dependent variables were analyzed via linear regression. The average 24-h THI was higher in Florida compared with California (90 vs. 72, respectively); the main driver of the higher THI in Florida was the high relative humidity (average 85.6% in Florida vs. 36.7% in California). In Florida, the rectal temperature of slick calves was 0.4°C lower than non-slick calves (39.5 ± 0.1 vs 39.9 ± 0.1°C); no differences were detected between slick and non-slick calves in California. Regardless of genotype, heifer calves in Florida had higher respiration rate, higher rectal and skin temperatures, and lower sweating rate than in California. This study is the first to evaluate physiological responses of calves carrying the SLICK1 allele under heat stress conditions in different climates. Our findings demonstrate that the presence of this allele is associated with lower rectal temperatures in pre- and post-weaning Holstein females. According to the physiological parameters evaluated, calves raised in Florida appeared to be under more severe heat stress; in those conditions, the SLICK1 allele was advantageous to confer thermotolerance as evidenced by lower rectal temperature in slick animals.

Antibiotic use and potential economic impact of implementing selective dry cow therapy in large US dairies.

In this study, our objectives were to evaluate the economic feasibility of implementing selective dry cow therapy (SDCT) in large US herds and to estimate the potential reduction in antibiotic use around the dry period if SDCT management is adopted. Cow-level data were obtained from the Dairy Herd Improvement Association (AgriTech, Visalia, CA) and individual dairy herds in California. Logistic regression models were used to predict the incidence risk of subclinical and clinical mastitis in the subsequent lactation for 96 last test-day somatic cell score categories. Linear programming was used to optimize the costs of dry cow therapy in 3 simulated large US dairy herds with different bulk tank somatic cell counts (BTSCC). The objective function was aimed at minimization of the total cost of mastitis around the dry period (TCMD), under a varying constraint of the maximum percentage of cows dried off with antibiotics. A sensitivity analysis was performed on milk price, dry-off antibiotic price, and risk ratio of mastitis in the subsequent lactation when no antibiotics and only teat sealant was used at dry-off. For all situations, blanket dry cow therapy was more expensive than SDCT. In a herd with medium BTSCC, the TCMD was $54.7 per primiparous dry cow and $58.5 per multiparous dry cow annually. In the optimal economic situation where SDCT was used, only 30% of primiparous cows received antibiotics, leading to a TCMD of $52.4 per primiparous dry cow, whereas 88% of multiparous cows received antibiotics, at a cost of $58.2 per multiparous dry cow. This corresponded with an overall reduction of 29% in the use of antibiotics around the dry period in a conservative scenario. This study showed that it is economically feasible to reduce antibiotic use associated with dry cow therapy in large US dairy herds. This contributes to the efforts of reducing antibiotic use worldwide.

Distribution of seasonality of calving patterns and milk production in dairy herds across the United States.

Calving patterns and milk production are seasonal throughout the United States; however, the distribution of seasonality, and the extent to which this seasonality is due to direct effects of climate on milk production and reproductive performance or farm management, is not well quantified. Summer-to-winter (SW) ratios have been used as measures of seasonality, but other measures such as low-to-peak (LP) ratios have been proposed. Our objectives were (1) to describe the distribution of seasonality in calving pattern and milk production among herds in the United States, (2) to compare SW and LP ratios of calving pattern and milk production, (3) to quantify the effect of a seasonal calving pattern, parity, and percentage of dry cows on seasonality of milk production, and (4) to describe the association between seasonality in calving pattern and milk production, herd size, and daily milk production per cow. The final data set contained Dairy Herd Improvement Association lactation records from 2015 from 5,292 (calving pattern) and 5,200 (milk production) herds for 41 states in the United States. We used generalized linear regression models with 1 sinusoidal curve to model calving pattern and milk production per cow for each herd. For milk production, a model adjusting for days in milk (DIM) and the interaction of DIM and parity (ADJ) and a model that was not adjusted (NO) were run. Both models included the effect of the percentage of dry cows. We used SW and LP ratios calculated from the parameters of the sinusoidal component of the models as measures of seasonality. The variability within states for all seasonality measures was large. The median LP ratio of calving pattern was 0.61, and small herds were more seasonal (LP ratio 0.56) than large herds (LP ratio 0.75). For milk production, the median LP ratio-NO was 0.88, and the LP ratio-ADJ was 0.90. Small herds were more seasonal (0.89) than large herds (0.92) when their LP ratios-ADJ were compared. States in the south of the United States were the most seasonal for calving patterns and milk production. Adjusting for DIM and parity increased the LP ratio of milk production by 8.9% for 66% of the herds. Adjusting for the percentage of dry cows increased the LP ratio in 72.9% of the herds by a median value of 21.8%. The correlations between SW and LP ratios were weak. Herds that were more seasonal for milk production had a lower average daily milk per cow than less-seasonal herds. In conclusion, seasonality in calving patterns and milk production among herds varied greatly across the United States. Sinusoidal models with covariates allowed for quantification of the effects of calving pattern, DIM, and parity on the seasonality in milk production. The LP ratios captured the maximum seasonality better than SW ratios did.

Impact of Partially Substituting Canola Meal with Solvent-Extracted Distillers Grain with Solubles as a Protein Source on Milk Production in a Commercial Holstein Dairy Herd.

The removal of corn oil from dried distillers grains using solvent extraction allows a higher level of inclusion for solvent-extracted dried distillers grains with solubles (SDG) in rations and reduces the risk of milk fat depression in lactating Holstein dairy cows. The objective of this study was to evaluate the impact of substituting 70% of the canola meal (CM) with SDG on milk production and total mixed ration costs. A total of 1408 Holstein cows averaging 91 ± 49 days in milk were randomly enrolled to one of four dietary treatment groups: (1) primiparous control cows (PC) fed 13% CM and 0.21% urea; (2) primiparous SDG cows (PSDG) fed 4.2% CM, 8.8% SDG and 0.42% urea; (3) multiparous control cows (MC) with 13% CM and 0.21% urea; and (4) multiparous SDG cows (MSDG) with 4.1% CM, 8.6% SDG and 0.42% urea. The total mixed rations were formulated to be isonitrogenous. For the income over the feed costs from a control herd, the fed PC and MC's total mixed rations and the fed PSDG and MSDG's total mixed rations were compared. The milk yield, energy-corrected milk, milk fat yield, milk protein yield and milk protein % were lower in the PC cows compared to the PSDG cows. The MSDG cows scored lower in terms of the milk yield, milk protein yield and milk protein % and higher for the 3.5%-fat-corrected milk, milk fat yield and milk fat % compared to the MC cows. The total income, cost of dry matter and income over feed costs per cow/d were higher in the control vs. SDG simulated dairy herds. The control herd had a higher income over feed costs than the SDG herd because the average milk yield per cow/d was higher even though the SDG herd had a lower total mixed ration cost and higher milk fat production.

Potential impacts to antibiotics use around the dry period if selective dry cow therapy is adopted by dairy herds: An example of the western US.

Mastitis is a prevalent and expensive disease in dairy herds worldwide. Blanket dry cow therapy (BDCT), in which all quarters of all cows are infused with antimicrobials at the dry-off, is a cornerstone for mastitis control in many countries. An alternative approach is the use of selective dry cow therapy (SDCT), in which only cows with high risk for intramammary infection (IMI) at dry-off receive antimicrobials. Our objectives in this cross-sectional study were to estimate the potential reduction in the use of antimicrobials if SDCT was adopted in the US by using cow-level dairy herd data and to describe the factors associated with cows being classified as high-risk for an IMI at dry-off. Besides, we aimed to describe the seasonality in IMI at dry-off. We used cow-level somatic cell score (SCS) test-day data from herds in the western US (DHIA, Dairy Herd Improvement Association, AgriTech, Visalia, CA) to create five scenarios to classify cows as high risk for IMI at dry-off. Associations between cow-level data and state were also used in logistic regression models. We also calculated the average animal-defined daily dosage of antimicrobials per cow per year around the dry period if a BDCT or SDCT approach is used, adjusting for the risk of cases of clinical mastitis in the next lactation for the SDCT approach. The point prevalence of IMI at the last test-day before dry-off varied between 15.0 % and 54 % for primiparous and 34.0 % and 85 % for multiparous cows, depending on the scenario. By extrapolating our results obtained from using data from dairies enrolled in the DHIA testing program for the western US, we demonstrated that regardless of the criteria used to classify cows as high risk of IMI at dry-off (scenarios 1-5), if selective dry cow therapy is adopted in the US, the dairy industry could reduce the use of antimicrobials around the dry-off between 31 % and 66 %. Multiparous cows had greater odds of being classified as high-risk than primiparous cows. Cows dried off in the spring, summer, and fall had lower odds of being classified as high-risk compared to cows dried off in the winter. Advanced days in milk at dry-off was associated with greater risk of IMI at dry-off. Greater milk yield and higher protein percentage at the last test-day before dry-off were associated with decreased odds of a cow being classified as high-risk at dry-off, cows in small herds had greater odds of being classified as high-risk at dry-off, and a variation among states was observed.

Intercomparación de las medidas de actividad por parte de los usuarios en diferentes servicios medicina nuclear de Sergipe / Intercomparasion of activity measurements in different nuclear medicine centers of Sergipe, Brazil

Un programa de control de calidad en medicina nuclear incluye la verificación de la eficiencia de equipos utilizados para el diagnóstico y tratamiento, hasta la formación. En este estudio se evaluaron las medidas de actividad realizada por los usuarios que trabajan en los servicios de medicina nuclear en Sergipe, Brazil. En las medidas de actividad se estableció uno y se caracteriza entre las medidas de fuentes radiactivas selladas de actividad normal, 133Ba y 57Co, llevada a cabo por distintos profesionales, con el fin de comprobar la reproducibilidad de estas medidas cuando se compara con el valor por defecto de la actividad de cada fuente. Los resultados de inter-medidas en estado satisfactorio y pueden ser utilizados como parámetros en el evaluación futuro de los servicios de medicina nuclear.

A program of quality control program in nuclear medicine includes the verification of the efficiency of any equipment used for diagnosis and treatment, until the personal training. In this study were evaluated measures of activity undertaken by users who work in nuclear medicine services in Sergipe, Brazil. In measures of activity was established and characterized an inter measures of activity sealed radioactive sources standard, 133Ba and 57Co, carried out by different professionals, aiming to verify the reproducibility of these measures when compared with the default value of the activity of each source. The results of inter-measures were in satisfactory and can be used as parameters in future evaluation services in nuclear medicine.