RESUMO
The presence of IgG and IgA antibodies to Simkania negevensis in adult Italian patients with respiratory or gastrointestinal symptoms was investigated by the microimmunofluorescence test. In patients with respiratory infections, IgG (50%) and IgA (13%) seropositivity was consistent with previous data. In patients with gastrointestinal disorders, IgG (68%) and IgA (18%) seroprevalence was significantly higher than in healthy controls. These results, in association with the previously described detection of S. negevensis in water sources, could suggest an oral route of infection other than droplets or close contact, and a possible association of S. negevensis with gastrointestinal infections.
Assuntos
Anticorpos Antibacterianos/sangue , Chlamydiales/imunologia , Gastroenteropatias/microbiologia , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Infecções Respiratórias/microbiologia , Adulto , Idoso , Estudos de Casos e Controles , Reações Cruzadas , Imunofluorescência , Infecções por Bactérias Gram-Negativas/imunologia , Humanos , Imunoglobulina A/biossíntese , Imunoglobulina G/biossíntese , Itália , Pessoa de Meia-Idade , Estudos Retrospectivos , Estudos SoroepidemiológicosRESUMO
In the present study, a monoclonal antibody (mAb), D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. negevensis-infected cells by using the immunofluorescence test. D5-14 mAb, reacting in immunoblot with an approximately 64-66-kDa protein of C. trachomatis LGV2 serotype, recognized a protein with the same molecular mass when tested with S. negevensis elementary bodies.
Assuntos
Chlamydia trachomatis/imunologia , Chlamydiales/isolamento & purificação , Anticorpos Antibacterianos/sangue , Anticorpos Monoclonais/imunologia , Western Blotting , Técnicas de Cultura de Células/métodos , Chlamydiales/imunologia , Imunofluorescência , HumanosRESUMO
The in vitro activity of six cathelicidin peptides against the reference strain Z of Simkania negevensis was investigated. Five peptides-PG-1, Bac7, SMAP-29, BMAP-27, and BMAP-28-proved to be active at very low concentrations (1 to 0.1 µg/mL), while LL-37 cathelicidin was ineffective even at a concentration of 100 µg/mL. In comparison to chlamydiae, S. negevensis proved to be more susceptible to the antimicrobial peptides tested.