RESUMO
Animal testing is crucial in situations when research on humans is not allowed because of unknown health risks and ethical concerns. The current project aims to develop reporting guidelines exclusively for animal studies in Endodontology, using an established consensus-based methodology. The guidelines have been named: Preferred Reporting Items for Animal Studies in Endodontology (PRIASE) 2021. Nine individuals (PD, VN, AK, PM, MN, JF, EP, JJ and SJ), including the project leaders (PD, VN) formed a steering committee. The steering committee developed a novel checklist by adapting and integrating their animal testing and peer review experience with the Animals in Research: Reporting In Vivo Experiments (ARRIVE) guidelines and also the Clinical and Laboratory Images in Publications (CLIP) principles. A PRIASE Delphi Group (PDG) and PRIASE Online Meeting Group (POMG) were also formed. Thirty-one PDG members participated in the online Delphi process and achieved consensus on the checklist items and flowchart that were used to formulate the PRIASE guidelines. The novel PRIASE 2021 guidelines were discussed with the POMG on 9 September 2020 via a Zoom online video call attended by 21 individuals from across the globe and seven steering committee members. Following the discussions, the guidelines were modified and then piloted by several authors whilst writing a manuscript involving research on animals. The PRIASE 2021 guidelines are a checklist consisting of 11 domains and 43 individual items together with a flowchart. The PRIASE 2021 guidelines are focused on improving the methodological principles, reproducibility and quality of animal studies in order to enhance their reliability as well as repeatability to estimate the effects of endodontic treatments and usefulness for guiding future clinical studies on humans.
Assuntos
Endodontia , Projetos de Pesquisa , Animais , Consenso , Humanos , Reprodutibilidade dos Testes , Relatório de PesquisaRESUMO
Laws and ethics require that before conducting human clinical trials, a new material, device or drug may have to undergo testing in animals in order to minimize health risks to humans, unless suitable supporting grandfather data already exist. The Preferred Reporting Items for Animal Studies in Endodontology (PRIASE) 2021 guidelines were developed exclusively for the specialty of Endodontology by integrating and adapting the ARRIVE (Animals in Research: Reporting In Vivo Experiments) guidelines and the Clinical and Laboratory Images in Publications (CLIP) principles using a validated consensus-based methodology. Implementation of the PRIASE 2021 guidelines will reduce potential sources of bias and thus improve the quality, accuracy, reproducibility, completeness and transparency of reports describing animal studies in Endodontology. The PRIASE 2021 guidelines consist of a checklist with 11 domains and 43 individual items and a flowchart. The aim of the current document is to provide an explanation for each item in the PRIASE 2021 checklist and flowchart and is supplemented with examples from the literature in order for readers to understand their significance and to provide usage guidance. A link to the PRIASE 2021 explanation and elaboration document and PRIASE 2021 checklist and flowchart is available on the Preferred Reporting Items for study Designs in Endodontology (PRIDE) website (http://pride-endodonticguidelines.org/priase/).
Assuntos
Endodontia , Projetos de Pesquisa , Animais , Lista de Checagem , Humanos , Reprodutibilidade dos Testes , Relatório de PesquisaRESUMO
The assessment of root canal curvature is essential for clinical and research purposes. This systematic review presents an overview of the published techniques for the measurement of root canal curvature features using imaging and to provide a critique of their clinical application. A database search in PubMed, PubMed Central, Embase, Scopus, EBSCO Dentistry & Oral Sciences Source and Virtual Health Library was conducted, using appropriate key words to identify measurement methods for root canal curvatures. The search strategy retrieved 10594 records in total, and 31 records fulfilled the inclusion criteria. From 2D image acquisitions, eleven studies measured exclusively the angle of curvature, an additional thirteen measured other curvature features (level, height, radius, length and shape). Seven reports described methods from 3D imaging (CBCT, µCT). Root canal curvatures should be measured, for clinical proposes, to facilitate endodontic treatment planning, and in research, to reduce the risk of selection bias. This review has revealed that there are many methods described in the literature; however, no consensus exists on which method should be used. Some of the methodologies have potential clinical translation, whereas others are suitable for research purpose only, as they require a specific software or radiographic exposure in the mesiodistal direction.
Assuntos
Cavidade Pulpar/anatomia & histologia , Diagnóstico por Imagem/métodos , Odontometria/métodos , Raiz Dentária/anatomia & histologia , Bases de Dados Factuais , Cavidade Pulpar/diagnóstico por imagem , Humanos , Processamento de Imagem Assistida por Computador/métodos , Imageamento Tridimensional/métodos , Metanálise como Assunto , Radiografia Dentária/métodos , Software , Raiz Dentária/diagnóstico por imagemRESUMO
The regulated use of animals in endodontic research is often necessary to investigate the biological mechanisms of endodontic diseases and to measure the preclinical efficacy, biocompatibility, toxicology and safety of new treatments, biomaterials, sealers, drugs, disinfectants, irrigants, devices and instruments. Animal testing is most crucial in situations when research on humans is not ethical, practical or has unknown health risks. Currently, there is a wide variability in the quality of manuscripts that report the results of animal studies. Towards the goal of improving the quality of publications, guidelines for preventing disability, pain, and suffering to animals, and enhanced reporting requirements for animal research have been developed. These guidelines are referred to as Animals in Research: Reporting In Vivo Experiments (ARRIVE). Henceforth, causing any form of animal suffering for research purposes is not acceptable and cannot be justified under any circumstances. The present report describes a protocol for the development of welfare and reporting guidelines for animal studies conducted in the specialty of Endodontology: the Preferred Reporting Items for Animal Studies in Endodontology (PRIASE) guidelines. The PRIASE guidelines will be developed by adapting and modifying the ARRIVE guidelines and the Clinical and Laboratory Images in Publication (CLIP) principles. The development of the new PRIASE guidelines will include a five-step consensus process. An initial draft of the PRIASE guidelines will be developed by a steering committee. Each item in the draft guidelines will then be evaluated by members of a PRIASE Delphi Group (PDG) for its clarity using a dichotomous scale (yes or no) and suitability for its inclusion using a 9-point Likert scale. The online surveys will continue until each item achieves this standard, and a set of items are agreed for further analysis by a PRIASE Face-to-face Consensus Meeting Group (PFCMG). Following the consensus meeting, the steering committee will finalize and confirm the PRIASE guidelines taking into account the responses and comments of the PFCMG. The PRIASE guidelines will be published and disseminated internationally and updated periodically based on feedback from stakeholders.
Assuntos
Endodontia , Projetos de Pesquisa , Animais , Consenso , Humanos , Dor , Relatório de PesquisaRESUMO
The role and effect of glide path preparation in root canal treatment remain controversial. This systematic review aims to compare apical transportation and canal centring of different glide path preparation techniques, with or without subsequent engine-driven root canal preparation. A database search in PubMed, PubMed Central, Embase, Scopus, EBSCO Dentistry & Oral Sciences Source and Virtual Health Library was conducted, using appropriate key words to identify the effect of glide path preparation (or its absence) on apical transportation and canal centring. An assessment for the risk of bias in included studies was carried out. Amongst 2146 studies, 18 satisfied the inclusion criteria. Nine studies assessed glide path preparation per se, comparing apical transportation and canal centring of rotary systems and/or manual files; eleven further investigations examined the efficacy of the glide path prior to final canal preparation with different engine-driven systems. Risk of bias and other study design features with potential influence on study outcomes and clinical implications were assessed. Based on the available evidence, and within the limitation of the studies included, preparation of a glide path using rotary sequences performs similarly (in most of the component studies) or significantly better than manual preparation when assessing apical transportation or canal centring. When compared to the absence of a glide path, canal shaping following glide path preparation was of similar, or significantly better quality, in regard to apical transportation or canal centring.
Assuntos
Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Tratamento do Canal Radicular/instrumentação , Tratamento do Canal Radicular/métodos , Bases de Dados Factuais , Ligas Dentárias , Cavidade Pulpar , Desenho de Equipamento , Humanos , Rotação , Ápice DentárioRESUMO
AIM: To evaluate the influence of different apical enlargement protocols on the radiographic and histological healing of apical periodontitis in rats. METHODOLOGY: Apical periodontitis was induced bilaterally in the mandibular right and left first molars of 24 Wistar rats by pulp exposure to the oral cavity for 3 weeks. A standard serial root canal preparation technique was performed in the molar of one side, whilst the opposite side was the control group. Rats were randomly divided into three experimental groups (n = 8), according to the diameter of apical enlargement during root canal preparation: K-files size 20 (EG1), size 25 (EG2) and size 30 (EG3). Each animal was its own positive control, because the opposite arch remained untreated. Root canals were filled with a standard technique. After 3 weeks, the animals were euthanized. The main outcome of apical periodontitis healing was evaluated radiographically (mm2 ) and histologically (ordinal scores of inflammation) using a HE staining technique. The measurement of effect was obtained between the three experimental groups by carrying out generalized estimating equations, with Poisson regression with robust variance, pairing each experimental group with its respective control group within animals, adjusted for the mean within animal differences, with α = 5%. RESULTS: The mean and standard deviations of radiographic apical periodontitis size (mm2 ) and intensity of histological inflammatory scores were, respectively: EG1 (0.44 ± 0.27; 2.25 ± 0.46), EG2 (0.33 ± 0.10; 2.50 ± 0.53) and EG3 (0.22 ± 0.08; 2.63 ± 0.74). After 3 weeks, a significantly more favourable radiographic repair was observed when larger apical enlargement was performed (EG3), compared to EG1 and EG2 (P = 0.001). All experimental groups were associated with a significant difference on the radiographic and histological healing of apical periodontitis compared with its respective control group. CONCLUSION: Under the experimental conditions of this study, a larger apical enlargement protocol favoured a more rapid radiographic repair of apical periodontitis in rats after a 3-week follow-up.
Assuntos
Periodontite Periapical/patologia , Periodontite Periapical/terapia , Tecido Periapical/patologia , Ápice Dentário/patologia , Animais , Reabsorção Óssea/diagnóstico por imagem , Reabsorção Óssea/patologia , Cavidade Pulpar/patologia , Feminino , Inflamação , Mandíbula , Dente Molar , Periodontite Periapical/diagnóstico por imagem , Tecido Periapical/diagnóstico por imagem , Ratos , Ratos Wistar , Materiais Restauradores do Canal Radicular , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Tratamento do Canal Radicular/métodos , Ápice Dentário/diagnóstico por imagem , Cicatrização/fisiologiaRESUMO
AIM: To determine the prevalence of preoperative endodontic pain (PREP) and the incidence of postoperative endodontic pain (POEP), identifying the predictors of PREP and POEP in a southern Brazilian subpopulation, using clinical data from an electronic chart database (ECD). METHODOLOGY: This retrospective observational study included 563 consecutive individuals presenting for root canal treatment (RCT). Patients were treated by undergraduate and graduate students, following standard RCT protocols. Demographic, medical and dental variables were extracted from a pre-structured and standardized ECD. The main outcomes PREP and incident POEP were collected through a 0-10 numeric rating scale, dichotomized as none/mild (<4) or moderate/severe (≥4) pain. Predictive models calculating the prevalence ratios (PR) of PREP and the relative risks (RR) of incident POEP were carried out with Poisson regression analysis, estimating the relationship between clinical factors, PREP and incident POEP. RESULTS: Mean age at baseline was 49.2 ± 17.1 years, with 68.4% women. The prevalence and incidence of moderate/severe PREP and POEP were 44.4% and 3.8%, respectively. RCT intervention significantly reduced PREP (P < 0.001). Multivariate analysis revealed that group of teeth, location (mandibular teeth), pulpitis, necrotic pulp, preoperative swelling and periapical radiolucency were independently associated with moderate/severe PREP, whilst age ≥60 years and root canal retreatments were independent protective factors to PREP (P < 0.05). No demographic, medical or dental variables were associated with POEP, although molar teeth (RR = 4.23, 95%CI = 0.93-19.2, P = 0.056) had a borderline nonsignificant association. CONCLUSIONS: Moderate/severe PREP was independently associated with age, group of teeth, location, preoperative swelling, retreatments and pulp and periapical status. No demographic, medical or dental variable predicted moderate/severe POEP following RCT amongst this subpopulation.
Assuntos
Doenças da Polpa Dentária/fisiopatologia , Doenças da Polpa Dentária/cirurgia , Polpa Dentária/fisiopatologia , Dor Facial/etiologia , Dor Pós-Operatória/etiologia , Tratamento do Canal Radicular/efeitos adversos , Brasil/epidemiologia , Registros Eletrônicos de Saúde , Dor Facial/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Dor Pós-Operatória/epidemiologia , Prevalência , Estudos RetrospectivosRESUMO
AIM: To evaluate the effects of topical Resolvin E1 (RvE1) application on infected dental pulps. METHODOLOGY: Forty-two male Wistar rats (n = 6 per three groups/and two time periods) were used. To induce inflammation, pulps in mandibular right first molars were accessed and then left exposed to the oral environment for 24 h. After this period, topical medication with a corticosteroid/antibiotic blend, or RvE1, or its vehicle (Ethanol 0.1%) was directly applied onto the pulp tissue and teeth were restored with silver amalgam. The effects of the protocols were evaluated histologically and compared with control pulps not exposed to the oral environment. The inflammatory changes after 24 and 72 h were assessed through a scoring method and analysed using the Kruskal-Wallis test followed by Dunn's. Differences were considered significant if P < 0.05 (CI = 95%). RESULTS: Ethanol and corticosteroid/antibiotic treatment were not effective in arresting severe inflammatory alterations of exposed pulps at 24 and 72 h (P < 0.05, CI = 95%). At both time periods, RvE1 treatment led to a reduction of tissue cellularity and extent of inflammation, whose changes were not different from control pulps (P > 0.05, CI = 95%). CONCLUSIONS: A protective role for RvE1 in pulp inflammation was observed even in the presence of contamination, suggesting that it may be a candidate for a novel therapeutic strategy for conservative dental pulp treatment.
Assuntos
Polpa Dentária/efeitos dos fármacos , Ácido Eicosapentaenoico/análogos & derivados , Corticosteroides/administração & dosagem , Corticosteroides/farmacologia , Animais , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Polpa Dentária/patologia , Ácido Eicosapentaenoico/farmacologia , Masculino , Ratos , Ratos WistarRESUMO
AIM: To evaluate if the immersion of chlorhexidine-impregnated gutta-percha points in chlorine-containing endodontic irrigants causes colour changes and precipitate formation. METHODOLOGY: Eighty-one size 25 chlorhexidine medicated (Activ Points, Roeko, Langenau, Germany) and 27 size 25 standard gutta-percha points (Roeko) were immersed in microtubes, containing the following solutions: 0.5 and 5.25% NaOCl or Aquatine (Optident, Ilkley, UK). The samples were visually assessed, by two independent observers, at regular intervals over 3 weeks, to detect colour changes and precipitate formation. A score system was used to grade the precipitate intensity. To compare the number of days required for a precipitate to form mean and standard differences were calculated; the means between the groups were compared using a one-way analysis of variance test. Mean intensity scores were calculated and compared between groups using Kruskal-Wallis procedure followed by the Mann-Whitney test. RESULTS: The groups containing 5.25% NaOCl started to produce a visible precipitate after fourteen days (16.48 ± 0.98) for the Activ points; this process was first noticed with the standard gutta-percha group on day 17 (18.33 ± 1.22 days), with a statistically significant difference (P = 0.002). When looking at the intensity scores from day 17 onwards, these two groups differed statistically, with a greater amount for the AP group (P < 0.001). No other test group presented with changes. CONCLUSION: The placement of impregnated gutta-percha points, in 5.25% NaOCl for a 2-week period, caused the formation of a precipitate in the experimental conditions of this study.
Assuntos
Anti-Infecciosos Locais/química , Clorexidina/química , Cloro/química , Guta-Percha/química , Materiais Restauradores do Canal Radicular/química , Irrigantes do Canal Radicular/química , Precipitação Química , Cor , Desinfetantes de Equipamento Odontológico/química , Humanos , Peróxido de Hidrogênio/química , Ácido Hipocloroso/química , Teste de Materiais , Hipoclorito de Sódio/química , Fatores de TempoRESUMO
Sodium hypochlorite (NaOCl) is recommended as an endodontic irrigant in view of its broad antimicrobial and tissue dissolution capacities. To enhance its penetration into inaccessible areas of root canals and to improve its overall effect, the addition of surface-active agents has been suggested. The aim of this investigation was to review the effect of the reduction of the surface tension on the performance of NaOCl in endodontics. A search was performed in the Medline electronic database (articles published up to 28 July 2012, in English) with the search terms and combinations as follows: 'sodium hypochlorite AND surface tension or interfacial force or interfacial tension or surface-active agent or amphiphilic agent or surface active agent or surfactant or tenside or detergent'. The purpose of this search was to identify publications that compared NaOCl alone and NaOCl modified with the addition of a surface-active agent in endodontics. A hand search of articles published online ('in-press' and 'early view'), and appearing in the reference list of the articles included, was further performed, using the same search criteria as the electronic search. The search identified 302 publications, of which 11 fulfilled the inclusion/exclusion criteria of the review. The evidence available suggests that surface-active agents improve the penetration of NaOCl in the main canal and have no effect on its pulp tissue dissolution ability. There are, however, insufficient data to enable a sound conclusion to be drawn regarding the effect of modifying NaOCl's surface tension on lubrication, antimicrobial and smear layer or debris removal abilities.
Assuntos
Irrigantes do Canal Radicular/química , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio/química , Tensoativos/química , Antibacterianos/química , Antibacterianos/farmacocinética , Cavidade Pulpar/metabolismo , Cavidade Pulpar/ultraestrutura , Humanos , Irrigantes do Canal Radicular/farmacocinética , Hipoclorito de Sódio/farmacocinética , Solventes/química , Solventes/farmacocinética , Tensão Superficial , Tensoativos/farmacocinéticaRESUMO
AIM: To present the case of a maxillary left lateral incisor with Oehlers' type III dens invaginatus in which cone beam computed tomography (CBCT) was used as an adjunctive resource in the diagnosis as well as in the planning and 2-year follow-up of the nonsurgical/surgical treatment. SUMMARY: The tooth had two root canals: a primary (main) canal with vital pulp that appeared to be closed apically and an invaginated canal that was necrotic, wide-open at the portal of exit and associated with a large chronic periapical lesion extending to the apex of the maxillary left central incisor. Radiographic tracking of a sinus tract in the labial gingiva of the affected tooth with a gutta-percha point revealed its origin to be the invagination. The CBCT scans revealed that the periapical radiolucency was significantly larger than seen radiographically as well as an increased thickness of the buccal cortical plate. Conventional root canal treatment of the primary canal was undertaken. As nonsurgical access to the invaginated canal was not possible, endodontic surgery was performed for curettage of the lesion, root-end cavity preparation using ultrasonic tips and root canal filling with white mineral trioxide aggregate (MTA). CBCT scanning after 17 months and clinical and radiographic follow-up after 24 months revealed complete periapical repair and absence of symptoms. KEY LEARNING POINTS: The combination of nonsurgical and surgical treatments produced periapical repair in a tooth with type III dens invaginatus with two root canals. CBCT may aid the diagnosis as well as the management plan and follow-up of teeth with this developmental anomaly.
Assuntos
Tomografia Computadorizada de Feixe Cônico , Dens in Dente/diagnóstico por imagem , Incisivo/anormalidades , Planejamento de Assistência ao Paciente , Compostos de Alumínio/uso terapêutico , Compostos de Cálcio/uso terapêutico , Criança , Dens in Dente/terapia , Fístula Dentária/diagnóstico por imagem , Fístula Dentária/terapia , Cavidade Pulpar/anormalidades , Cavidade Pulpar/diagnóstico por imagem , Necrose da Polpa Dentária/diagnóstico por imagem , Necrose da Polpa Dentária/terapia , Combinação de Medicamentos , Seguimentos , Humanos , Incisivo/diagnóstico por imagem , Masculino , Óxidos/uso terapêutico , Periodontite Periapical/diagnóstico por imagem , Periodontite Periapical/terapia , Obturação Retrógrada/métodos , Materiais Restauradores do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/métodos , Tratamento do Canal Radicular/métodos , Silicatos/uso terapêuticoRESUMO
Chlorine-containing solutions are used for broad disinfection purposes. Water disinfection literature suggests that their disinfectant action depends on pH values as this will influence the available free chlorine forms. Hypochlorous acid (HOCl) has been suggested to have an antimicrobial effect around 80-100 times stronger than the hypochlorite ion. The aim of this paper was to review the influence of pH changes on the efficacy of chlorine-containing endodontic irrigating solutions. An electronic and hand search (articles published through to 2010, including 'in press' articles; English language; search terms 'root canal irrigants AND sodium hypochlorite or hypochlorous acid or superoxidized water or electrochemically activated solution'; 'antimicrobial action AND sodium hypochlorite or hypochlorous acid or superoxidized water or electrochemically activated solution'; 'tissue dissolution AND sodium hypochlorite or hypochlorous acid or superoxidized water or electrochemically activated solution'; 'smear layer AND sodium hypochlorite or hypochlorous acid or superoxidized water or electrochemically activated solution') was performed to identify publications that compared chlorine water solutions with different pH. Of 1304 publications identified, 20 were considered for inclusion in the review. The search resulted in the retrieval of articles studying sodium hypochlorite (NaOCl), superoxidized waters (SOW) and sodium dichloroisocyanurate (NaDCC). Regarding antimicrobial efficacy, the literature suggested that reducing the pH value of NaOCl to between 6 and 7.5 would lead to improved action; SOW was described as having a lower antimicrobial effect. The tissue dissolution activity NaOCl decreased when the pH reached values between 6 and 7.5; NaDCC and SOW had no clinically relevant tissue dissolution capability. Chlorine solutions of different characteristics appeared to have some cleaning efficacy although they should to be used in conjunction with chelating and/or detergent agents.
Assuntos
Anti-Infecciosos/química , Cloro/química , Concentração de Íons de Hidrogênio , Irrigantes do Canal Radicular/química , Irrigação Terapêutica/métodos , Desinfecção/métodos , Técnicas Eletroquímicas , Humanos , Ácido Hipocloroso/química , Camada de Esfregaço , Hipoclorito de Sódio/químicaRESUMO
AIM: To analyse the anatomy of the root canal system of maxillary premolars with three distinct roots using high-resolution computed tomography (µCT). METHODOLOGY: Ten three-rooted human maxillary premolars were scanned transversally from crown to apex at 42µm intervals using a high-resolution desktop µTC system (Skyscan 1072). The images were processed and analysed for the following features: cross section of the roots and canals in the apical, middle and coronal thirds; location of the apical foramen; distance from the pulp chamber roof to the bifurcation and trifurcation of the canals and anatomic variations of the root canal system. The results were expressed descriptively. RESULTS: The cross-sectional shape of canals was heterogeneous along the length of the roots. The location of the apical foramen varied, tending to lie palatally or distally. The buccal pulp horn was larger than the palatal. The mean distance from the most cervical region of the pulp chamber roof to the bifurcation and trifurcation of the canals was 3.13 and 5.08mm, respectively. CONCLUSIONS: Features of the internal anatomy of the pulp cavity of three-rooted maxillary premolars were identified with the use of µCT. The results revealed the heterogeneity of three-rooted maxillary premolars.
Assuntos
Dente Pré-Molar/anatomia & histologia , Cavidade Pulpar/anatomia & histologia , Anatomia Transversal , Dente Pré-Molar/diagnóstico por imagem , Cavidade Pulpar/diagnóstico por imagem , Humanos , Maxila , Tomografia Computadorizada por Raios X/métodosRESUMO
AIM: The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY: Nine root samples (seven teeth) were processed using either Eastman Dental Institute (EDI) (n = 4 teeth/4 roots) or Zurich (n = 3 teeth/5 roots) protocols. The roots were sectioned longitudinally; one root portion was viewed with SEM, descriptively dividing its length into apical, middle and coronal; semi-thin and ultra-thin transverse sections were viewed under LM and TEM from each third of the other root portion. Each root was therefore examined using all microscopy techniques. Observations of bacterial presence, description and distribution within the root canal lumen and root dentine were systematically recorded using pre-determined criteria. RESULTS: The Zurich technique gave a more predictable division of the root, but the surface was slightly smeared and demineralization was incomplete. The Eastman Dental Institute (EDI) approach appeared to provide better ultrastructural detail. Bacteria were detected in eight of the nine roots. Bacterial biofilms were commonly seen adhering to the root canal surface, containing various cellular morphotypes: rods, cocci, filaments and spirochaetes. Bacteria were more evident apically than coronally, associated with the canal wall but were more commonly evident coronally than apically within the dentinal tubules. Polymorphs (PMNs) were found in all the root thirds, especially apically, often numerous and walling off the bacterial biofilm from the remaining canal lumen. CONCLUSIONS: Both protocols had merits and de-merits. The combination of microscopy techniques offered complementary views of intra-radicular bacterial colonization. The perception of confinement of the host/microbial interface at the apical foramen is not entirely correct; PMNs may be found even in the coronal third of root canals containing necrotic pulp tissue.
Assuntos
Bactérias/ultraestrutura , Cavidade Pulpar/microbiologia , Periodontite Periapical/microbiologia , Bactérias/classificação , Biofilmes , Técnica de Descalcificação , Cavidade Pulpar/ultraestrutura , Necrose da Polpa Dentária/microbiologia , Necrose da Polpa Dentária/patologia , Dentina/microbiologia , Dentina/ultraestrutura , Eritrócitos/microbiologia , Eritrócitos/ultraestrutura , Técnicas Histológicas , Interações Hospedeiro-Patógeno , Humanos , Microscopia , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Neutrófilos/microbiologia , Neutrófilos/ultraestrutura , Periodontite Periapical/patologia , Inclusão do Tecido , Ápice Dentário/microbiologia , Ápice Dentário/ultraestruturaRESUMO
UNLABELLED: Taurodontism is the morpho-anatomical change in the shape of a tooth, which involves enlargement of the body of the tooth and shortening of the roots. It has been reported in permanent and deciduous teeth, in premolars and molars and associated with certain syndromes, particularly in those involving an ectodermal defect. It has clinical implications which are relevant to both the general dental practitioner and the orthodontist. CLINICAL RELEVANCE: This review compiles the available literature concerning taurodont teeth and highlights the management issues raised by the anomaly.
Assuntos
Cavidade Pulpar/anormalidades , Anormalidades Dentárias/patologia , Dente Pré-Molar/anormalidades , Humanos , Dente Molar/anormalidades , Odontometria , Raiz Dentária/anormalidadesRESUMO
Dens invaginatus is a rare malformation of the teeth resulting from the invagination of the tooth crown before biological mineralization occurs. In most cases, the thin or incomplete enamel lining of the invagination cannot prevent the entry of bacteria into the pulp, which leads to pulp necrosis with an eventual periapical inflammatory response. The treatment options include preventive sealing or filling of the invagination, root canal treatment, endodontic apical surgery and extraction. The root canal treatment of such teeth is often complicated because of their anatomical complexity. This case describes a successful non-surgical endodontic treatment of a maxillary lateral incisor with type 2 dens invaginatus with a large periradicular lesion. At follow-up examinations after 6 and 12-months, the tooth was asymptomatic and the healed lesion was evident radiographically
Assuntos
Dens in Dente/terapia , Fístula Dentária/terapia , Cavidade Pulpar/anormalidades , Incisivo/anormalidades , Tratamento do Canal Radicular/métodos , Adolescente , Dens in Dente/complicações , Dens in Dente/patologia , Fístula Dentária/complicações , Cavidade Pulpar/patologia , Cavidade Pulpar/cirurgia , Necrose da Polpa Dentária/etiologia , Necrose da Polpa Dentária/cirurgia , Humanos , Incisivo/cirurgia , Masculino , Maxila , Raiz Dentária/anormalidades , Raiz Dentária/patologia , Resultado do TratamentoRESUMO
Super-oxidised waters, particularly Sterilox(®), have been suggested for the disinfection of dental unit water lines and dental impression materials owing to their antimicrobial efficacy. One of the previously suggested characteristics is their short shelf life. The purpose of this investigation was to understand the effect of storage conditions on Sterilox(®)'s stability. Eight bottles (four completely full, four half-full) of freshly prepared solution were divided into four groups and subsequently stored by being either exposed to or protected from sunlight. The chlorine concentration was monitored using chlorine test strips until the concentration reached zero, or until the thirteenth week. Statistically significant differences between the groups exposed to sunlight and the non-exposed groups (p <0.001) were found. The mean loss of chlorine per day for the non-exposed samples was 1.01 mg/L, whilst the mean for the exposed samples was 2.42 mg/L. The presence of air did not affect the chlorine decomposition in the bottles. The results of this investigation indicate that when the solution is exposed to sunlight, the decrease of chlorine starts at day 4, whilst for the groups sheltered from sunlight, the process started after day 14. Therefore, Sterilox(®) solutions appear to be more stable than previously surmised.
Assuntos
Desinfetantes de Equipamento Odontológico/química , Peróxido de Hidrogênio/química , Ar , Cloro/análise , Cloro/efeitos da radiação , Desinfetantes de Equipamento Odontológico/análise , Desinfetantes de Equipamento Odontológico/efeitos da radiação , Estabilidade de Medicamentos , Armazenamento de Medicamentos , Humanos , Peróxido de Hidrogênio/análise , Peróxido de Hidrogênio/efeitos da radiação , Concentração de Íons de Hidrogênio , Teste de Materiais , Luz Solar , Fatores de TempoRESUMO
AIM: To evaluate the early and delayed effects of fractioned teletherapy (radiotherapy) on the dental pulps of rats using Co(60). METHODOLOGY: In group 1 - rats (n = 15) were subjected to fractioned teletherapy by 30 daily sessions fractioned in doses of 200 cGy day(-1), totaling 60 Gy and the rats were killed immediately after the final dose of irradiation; group 2 - same protocol but killed 30 days following the final irradiation dose; groups 3 (n = 7) and 4 (n = 8) - formed controls without irradiation. Following perfusion, the left mandible of each rat was dissected and processed for histopathology. Serial sections (5 microm) were obtained and stained with HE or picrosirius. Observations were recorded for the coronal pulp tissue. A blinded observer evaluated HE sections using pre-defined indices of inflammation, nuclear alterations and extracellular matrix (ECM) hyalinization. Images of sections stained with picrosirius were converted to black and white for analysis by image-pro plus; areas in black (collagen) were measured as percentage area. The pulps of mandibular incisors of the specimens prepared for transmission electron microscopy (TEM) were subjected to descriptive analysis. Magnifications of 6300 and 10000 x were used to observe 10 pulp fibroblasts from each group. RESULTS: No inflammatory reactions or modification of the ECM status were found (P = 0.428) in any specimens. The collagen content also displayed no significant changes (P = 0.067) as a result of treatment. Groups 1 and 2 displayed significantly more nuclear alterations than the control groups (P < 0.05). The bubble-like aspect was more pronounced in group 1, and the bubbles looked smaller in group 2. The ECM showed no differences in the hyalinization status and there were no differences in the collagen area within the pulps. Under TEM, the pulp fibroblasts in group 1 displayed nuclear alterations that resembled circular, oval or elongated perforations; perforations also appeared in the cytoplasm. CONCLUSION: Fractioned teletherapy is capable of producing nuclear alterations in the dental pulp tissue of rats.