RESUMO
SARS-CoV-2 infects less than 1% of cells in the human body, yet it can cause severe damage in a variety of organs. Thus, deciphering the non-cell-autonomous effects of SARS-CoV-2 infection is imperative for understanding the cellular and molecular disruption it elicits. Neurological and cognitive defects are among the least understood symptoms of COVID-19 patients, with olfactory dysfunction being their most common sensory deficit. Here, we show that both in humans and hamsters, SARS-CoV-2 infection causes widespread downregulation of olfactory receptors (ORs) and of their signaling components. This non-cell-autonomous effect is preceded by a dramatic reorganization of the neuronal nuclear architecture, which results in dissipation of genomic compartments harboring OR genes. Our data provide a potential mechanism by which SARS-CoV-2 infection alters the cellular morphology and the transcriptome of cells it cannot infect, offering insight to its systemic effects in olfaction and beyond.
Assuntos
Anosmia , COVID-19 , Animais , Cricetinae , Regulação para Baixo , Humanos , Receptores Odorantes , SARS-CoV-2 , OlfatoAssuntos
Academias e Institutos , Biologia/educação , Educação Continuada , Docentes , Viagem , Recursos HumanosRESUMO
The piriform cortex (PCX) is the largest component of the olfactory cortex and is hypothesized to be the locus of odor object formation. The distributed odorant representation found in PCX contrasts sharply with the topographical representation seen in other primary sensory cortices, making it difficult to test this view. Recent work in PCX has focused on functional characteristics of these distributed afferent and association fiber systems. However, information regarding the efferent projections of PCX and how those may be involved in odor representation and object recognition has been largely ignored. To investigate this aspect of PCX, we have used the efferent pathway from mouse PCX to the orbitofrontal cortex (OFC). Using double fluorescent retrograde tracing, we identified the output neurons (OPNs) of the PCX that project to two subdivisions of the OFC, the agranular insula and the lateral orbitofrontal cortex (AI-OPNs and LO-OPNs, respectively). We found that both AI-OPNs and LO-OPNs showed a distinct spatial topography within the PCX and fewer than 10% projected to both the AI and the LO as judged by double-labeling. These data revealed that the efferent component of the PCX may be topographically organized. Further, these data suggest a model for functional organization of the PCX in which the OPNs are grouped into parallel output circuits that provide olfactory information to different higher centers. The distributed afferent input from the olfactory bulb and the local PCX association circuits would then ensure a complete olfactory representation, pattern recognition capability, and neuroplasticity in each efferent circuit.
Assuntos
Córtex Piriforme/anatomia & histologia , Células Receptoras Sensoriais/citologia , Animais , Camundongos , Córtex Piriforme/citologiaRESUMO
Olfactory receptors are G protein-coupled receptors that mediate olfactory chemosensation and serve as chemosensors in other tissues. We find that Olfr78, an olfactory receptor expressed in the kidney, responds to short chain fatty acids (SCFAs). Olfr78 is expressed in the renal juxtaglomerular apparatus, where it mediates renin secretion in response to SCFAs. In addition, both Olfr78 and G protein-coupled receptor 41 (Gpr41), another SCFA receptor, are expressed in smooth muscle cells of small resistance vessels. Propionate, a SCFA shown to induce vasodilation ex vivo, produces an acute hypotensive response in wild-type mice. This effect is differentially modulated by disruption of Olfr78 and Gpr41 expression. SCFAs are end products of fermentation by the gut microbiota and are absorbed into the circulation. Antibiotic treatment reduces the biomass of the gut microbiota and elevates blood pressure in Olfr78 knockout mice. We conclude that SCFAs produced by the gut microbiota modulate blood pressure via Olfr78 and Gpr41.
Assuntos
Pressão Sanguínea/fisiologia , Intestinos/microbiologia , Rim/metabolismo , Metagenoma/fisiologia , Receptores Acoplados a Proteínas G/metabolismo , Receptores Odorantes/metabolismo , Renina/metabolismo , Transdução de Sinais/fisiologia , Animais , Biomassa , Pressão Sanguínea/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/genética , Hipertensão/genética , Hipertensão/metabolismo , Hipertensão/microbiologia , Mucosa Intestinal/metabolismo , Camundongos , Camundongos Knockout , Propionatos/metabolismo , Propionatos/farmacologia , Receptores Acoplados a Proteínas G/genética , Receptores Odorantes/genética , Transdução de Sinais/efeitos dos fármacos , Vasodilatação/efeitos dos fármacos , Vasodilatação/fisiologiaRESUMO
Membrane proteins are a large, diverse group of proteins, serving a multitude of cellular functions. They are difficult to study because of their requirement of a lipid membrane for function. Here we show that two-photon polarization microscopy can take advantage of the cell membrane requirement to yield insights into membrane protein structure and function, in living cells and organisms. The technique allows sensitive imaging of G-protein activation, changes in intracellular calcium concentration and other processes, and is not limited to membrane proteins. Conveniently, many suitable probes for two-photon polarization microscopy already exist.
Assuntos
Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Proteínas de Membrana/metabolismo , Proteínas de Membrana/ultraestrutura , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Microscopia de Polarização/métodos , Conformação Proteica , Relação Estrutura-AtividadeRESUMO
The nearly 2,000 glomeruli that cover the surface of the olfactory bulb are so distinctive that they were noted specifically in the earliest of Cajal's catalogues. They have variously been considered a functional unit, an organizational unit and a crucial component of the olfactory coding circuit. Despite their central position in olfactory processing, the development of the glomeruli has only recently begun to be investigated with new and powerful genetic tools. Some unexpected findings have been made that may lead to a new understanding of the processes involved in wiring sensory regions of the brain. It may no longer be sufficient to simply invoke genes, spikes and their interplay in the construction of brain circuits. The story of 'how the olfactory bulb got its glomeruli' may be more complex, and more revealing, than has been supposed.
Assuntos
Rede Nervosa/fisiologia , Bulbo Olfatório/citologia , Bulbo Olfatório/fisiologia , Neurônios Receptores Olfatórios/fisiologia , Potenciais de Ação/fisiologia , Animais , Humanos , Odorantes , Neurônios Receptores Olfatórios/citologia , Transdução de Sinais/fisiologiaRESUMO
A compelling new play revisits the discovery that drastically reduced maternal mortality.
RESUMO
Olfactory-like chemosensory signaling occurs outside of the olfactory epithelium. We find that major components of olfaction, including olfactory receptors (ORs), olfactory-related adenylate cyclase (AC3) and the olfactory G protein (G(olf)), are expressed in the kidney. AC3 and G(olf) colocalize in renal tubules and in macula densa (MD) cells which modulate glomerular filtration rate (GFR). GFR is significantly reduced in AC3(-/-) mice, suggesting that AC3 participates in GFR regulation. Although tubuloglomerular feedback is normal in these animals, they exhibit significantly reduced plasma renin levels despite up-regulation of COX-2 expression and nNOS activity in the MD. Furthermore, at least one member of the renal repertoire of ORs is expressed in a MD cell line. Thus, key components of olfaction are expressed in the renal distal nephron and may play a sensory role in the MD to modulate both renin secretion and GFR.
Assuntos
Túbulos Renais Distais/química , Rim/química , Mucosa Olfatória/química , Transdução de Sinais , Adenilil Ciclases/análise , Animais , Subunidades alfa de Proteínas de Ligação ao GTP/análise , Taxa de Filtração Glomerular , Rim/fisiologia , Camundongos , Camundongos Knockout , Neurônios Receptores Olfatórios , Renina/sangue , Renina/metabolismoRESUMO
The receptive field of the TRPA1 nociceptor is remarkably expansive when compared to other chemodetectors such as odorant receptors. The identification of a unique mechanism utilized by TRPA1 helps clarify how this protein can efficiently alert the cell to an array of reactive chemical agents, regardless of their structure.
Assuntos
Canais de Cálcio/química , Canais de Cálcio/fisiologia , Nociceptores/fisiologia , Receptores Odorantes/química , Receptores Odorantes/fisiologia , Animais , Anquirinas , Humanos , Odorantes , Dor/fisiopatologia , Ratos , Estimulação Química , Canal de Cátion TRPA1 , Canais de Cátion TRPCRESUMO
During normal and diseased aging, it is thought the capacity for tissue regeneration and repair in neuronal tissues diminishes. In the peripheral olfactory system, stem cell reservoirs permit regeneration of olfactory and vomeronasal sensory neurons, a unique capacity among neurons. Following injury, a large number of new neurons can be regenerated in a young animal. However, it is unknown whether this capacity for renewal exists in aged proliferative populations. Here, we report that neuronal replacement-associated proliferation continues in the vomeronasal organ of aged (18-24 months) mice. In addition, the potential for the aged stem cell to yield a mature neuron persisted at the same rate as that observed in young animals. Furthermore, the robust regenerative capacity to respond to both acute and sustained injury following olfactory bulbectomy remains intact even in very old animals. Hence, the neuronal epithelium lining the vomeronasal organ is unique in that it contains stem cells capable of generating functional neurons throughout life and in the aged animal in particular. This persistent regenerative capacity provides hope for neuronal replacement therapies in the aged nervous system.
Assuntos
Senescência Celular/fisiologia , Mucosa Nasal/fisiologia , Regeneração Nervosa/fisiologia , Neurônios/fisiologia , Mucosa Olfatória/fisiologia , Órgão Vomeronasal/fisiologia , Animais , Proliferação de Células , Camundongos , Camundongos Endogâmicos C57BL , Mucosa Nasal/citologia , Neurogênese/fisiologia , Neurônios/citologia , Mucosa Olfatória/citologia , Órgão Vomeronasal/citologiaRESUMO
As odorant receptors (ORs) are thought to be critical determinants of olfactory sensory neuron (OSN) axon targeting and organization, we examined the spatiotemporal onset of mice ORs expression from the differentiation of OSNs in the olfactory placode to an aging olfactory epithelium. ORs were first detected in the placode at embryonic day 9 (E9), at the onset of OSN differentiation but before axon extension. By E13, 22 of 23 ORs were expressed. Onset of individual OR expression was diverse; levels and patterns of expression were unique for each OR. Regional distribution of ORs within zones of the olfactory epithelium appeared stable across development; adult-like patterns were observed by E13. Finally, analysis of OR expression and chromosomal location suggests that ORs are not stochastically expressed; they show evidence of coordinated expression. Collectively, these studies demonstrate that ORs are not equally represented in the "olfactome" across an animal's lifespan.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Bulbo Olfatório/citologia , Neurônios Receptores Olfatórios/metabolismo , Receptores Odorantes/metabolismo , Algoritmos , Animais , Animais Recém-Nascidos , Diferenciação Celular/fisiologia , Mapeamento Cromossômico/métodos , Embrião de Mamíferos , Camundongos , Camundongos Endogâmicos C57BL , Análise em Microsséries/métodos , Moléculas de Adesão de Célula Nervosa/metabolismo , Bulbo Olfatório/embriologia , Bulbo Olfatório/crescimento & desenvolvimento , Receptores Odorantes/genética , Tubulina (Proteína)/metabolismoRESUMO
Olfaction relies on a coordinated partnership between odorant flow and neuronal communication. Disruption in our ability to detect odors, or anosmia, has emerged as a hallmark symptom of infection with SARS-CoV-2, yet the mechanism behind this abrupt sensory deficit remains elusive. Here, using molecular evaluation of human olfactory epithelium (OE) from subjects succumbing to COVID-19 and a hamster model of SARS-CoV-2 infection, we discovered widespread downregulation of olfactory receptors (ORs) as well as key components of their signaling pathway. OR downregulation likely represents a non-cell autonomous effect, since SARS-CoV-2 detection in OSNs is extremely rare both in human and hamster OEs. A likely explanation for the reduction of OR transcription is the striking reorganization of nuclear architecture observed in the OSN lineage, which disrupts multi-chromosomal compartments regulating OR expression in humans and hamsters. Our experiments uncover a novel molecular mechanism by which a virus with a very selective tropism can elicit persistent transcriptional changes in cells that evade it, contributing to the severity of COVID-19.
RESUMO
The discrimination of n-alkyl-saturated aldehydes during the early stage of odorant recognition by the rat I7 olfactory receptor (OR-I7) is investigated. The concentrations of odorants necessary for 50% activation (or inhibition) of the OR-I7 are measured by calcium imaging recordings of dissociated rat olfactory sensory neurons, expressing the recombinant OR-I7 from an adenoviral vector. These are correlated with the corresponding binding free energies computed for a homology structural model of the OR-I7 built from the crystal structure of bovine visual rhodopsin at 2.2 A resolution.
Assuntos
Aldeídos , Discriminação Psicológica , Receptores Odorantes/fisiologia , Aldeídos/química , Aldeídos/farmacologia , Animais , Bovinos , Discriminação Psicológica/efeitos dos fármacos , Modelos Moleculares , Odorantes , Ratos , Receptores Odorantes/química , Rodopsina/química , Células Receptoras SensoriaisRESUMO
Olfactory receptor (OR) genes constitute the basis for the sense of smell. It has long been observed that a subset of mammalian OR genes are expressed in nonolfactory tissues, in addition to their expression in the olfactory epithelium. However, it is unknown whether OR genes have alternative functions in the nonolfactory tissues. Using a dedicated microarray, we surveyed OR gene expression in olfactory epithelium as well as a number of nonolfactory tissues, in human and chimpanzee. Our observations suggest that ectopically expressed OR orthologous genes are expressed in the same nonolfactory tissues in human and chimpanzee more often than expected by chance alone. Moreover, we found that the subset of orthologous OR genes with conserved ectopic expression evolve under stronger evolutionary constraint than OR genes expressed exclusively in the olfactory epithelium. Thus, although we cannot provide direct functional data, our observations are consistent with the notion that a subset of ectopically expressed OR genes have additional functions in nonolfactory tissues.
Assuntos
Perfilação da Expressão Gênica , Receptores Odorantes/genética , Animais , Humanos , Neurônios Receptores Olfatórios , Análise de Sequência com Séries de Oligonucleotídeos , Pan troglodytes , Distribuição TecidualRESUMO
Olfactory responses to single odors have been well characterized but in reality we are continually presented with complex mixtures of odors. We performed high-throughput analysis of single-cell responses to odor blends using Swept Confocally Aligned Planar Excitation (SCAPE) microscopy of intact mouse olfactory epithelium, imaging ~10,000 olfactory sensory neurons in parallel. In large numbers of responding cells, mixtures of odors did not elicit a simple sum of the responses to individual components of the blend. Instead, many neurons exhibited either antagonism or enhancement of their response in the presence of another odor. All eight odors tested acted as both agonists and antagonists at different receptors. We propose that this peripheral modulation of responses increases the capacity of the olfactory system to distinguish complex odor mixtures.
Assuntos
Odorantes/análise , Neurônios Receptores Olfatórios/fisiologia , Olfato/fisiologia , Acetofenonas/análise , Monoterpenos Acíclicos/análise , Animais , Compostos de Benzil/análise , Camundongos , Camundongos Mutantes , Microscopia Confocal , Mucosa Olfatória/inervação , Análise de Célula ÚnicaAssuntos
Insetos , Ativação do Canal Iônico/efeitos dos fármacos , Receptores Odorantes/metabolismo , Animais , Cálcio/metabolismo , Proteínas de Drosophila/química , Proteínas de Drosophila/metabolismo , Condutividade Elétrica , Proteínas Heterotriméricas de Ligação ao GTP/metabolismo , Humanos , Ligantes , Nucleotídeos Cíclicos/metabolismo , Nucleotídeos Cíclicos/farmacologia , Odorantes/análise , Receptores Odorantes/química , Transdução de Sinais/efeitos dos fármacosRESUMO
Olfactory sensory neurons (OSNs) represent a unique population of neurons in which death and regeneration are ongoing throughout adulthood, a feature that makes them an attractive model cell type for the investigation of neuronal death. However, the mechanism by which OSNs die remains elusive. Therefore, we developed a culture system for studying pathways involved in OSN death. Here, we show that inhibition of transcription or translation, by actinomycin D or cycloheximide, respectively, suppresses pathways leading to death, prolonging the survival of OSNs in culture. We discovered that caspase activity and jun N-terminal kinase (JNK) signaling both play a role in OSN death, and inhibition of JNK activity suppresses effector caspase (caspase-3) activation. Results from studies in culture were confirmed in vivo, in a mouse bulbectomy-induced OSN death model. These findings provide new insights into the nature of OSN death and a means of studying OSNs in vitro.
Assuntos
Proteínas Quinases JNK Ativadas por Mitógeno/fisiologia , Sistema de Sinalização das MAP Quinases/fisiologia , Neurônios/enzimologia , Neurônios Receptores Olfatórios/enzimologia , Animais , Morte Celular/fisiologia , Células Cultivadas , Camundongos , Bulbo Olfatório/enzimologiaRESUMO
Dendrodendritic inhibition between mitral and granule cells in the olfactory bulb is thought to play an important role in olfactory discrimination. In this issue of Neuron, explore the propagation of action potentials along the secondary dendrites of mitral cells and their modulation by dendrodendritic inhibition.
Assuntos
Potenciais de Ação/fisiologia , Animais , Dendritos/fisiologia , Humanos , Bulbo Olfatório/fisiologia , Neurônios Receptores Olfatórios/fisiologia , Sinapses/fisiologiaRESUMO
A remarkable feature of peripheral olfactory projections in mammals is the convergence of axons from olfactory sensory neurons (OSNs) expressing the same odorant receptor (OR) into the same glomeruli. There is mounting evidence that the ORs play critical roles in glomerular formation. However, it remains unclear how the OR exerts its function of sorting axons into homogeneity. We and others have shown previously that activation of the G-protein/cAMP signaling cascade underlies glomerular formation. Here, we further investigated whether establishment of the mature glomerular array requires adenylyl cyclase 3 (AC3), a key component of the OR-mediated cAMP-dependent signaling cascade. We found robust AC3 expression in both OSN cilia and axons during the period of active glomerular formation in neonatal mice. Examination of OR-tagged mice in an AC3 knock-out background revealed that the absence of AC3 drastically and differentially perturbed the formation of several representative glomeruli. Furthermore, heterogeneous glomeruli innervated by axons of multiple OSN populations persisted in such mice well into adulthood. In addition, reproducible aberrations in axonal projections in AC3-/- mice appeared to correlate with the activation of specific OR loci, regardless of the expressed receptor sequence, suggesting that OR expression is but one factor in determining OSN axonal projections. Together, our results indicate that cAMP signaling is critical for axonal sorting and the establishment of axonal identity.