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Fibromyalgia is a chronic pain syndrome with diffuse pain, muscle weakness, and other symptoms. A relationship between the severity of symptoms and obesity has been observed. Objective: To determine the relationship between weight and the severity of fibromyalgia. Material and Methods: 42 patients with fibromyalgia were studied. BMI and fibromyalgia severity are classified weight by FIQR. Results: The mean age was 47.3 ± 9.4 years, 78% presented severe and extreme fibromyalgia, and 88% were overweight or obese. There was a positive correlation between the severity of the symptoms and the BMI (r = 0. 309). The reliability test for FIQR presented a Cronbach's α of 0.94. Conclusion: About 80% of the participants do not show controlled symptoms, and their prevalence of obesity is high, observing a positive correlation between both.
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Fibromialgia , Humanos , Adulto , Pessoa de Meia-Idade , Fibromialgia/complicações , Fibromialgia/epidemiologia , Reprodutibilidade dos Testes , Dor , Obesidade/complicações , Obesidade/epidemiologiaRESUMO
PURPOSE: To determine the frequency and association of polymorphisms in the TP53 and RB1 genes with clinical characteristics in a group of children with retinoblastoma (RB) in northern Mexico. METHODS: A prospective, longitudinal, and analytical study of 11 patients diagnosed with RB was conducted. Endpoint PCR and high-resolution real-time PCR were performed. Chi-square and Student t tests were used to evaluate associations between variables. Allelic frequencies, as well as genotypic and Hardy-Weinberg equilibriums, were evaluated using Guo and Thompson's method. RESULTS: We found a statistically significant difference between the polymorphism RB1-GG/rs9568036 and tumor chemoresistance (p<0.05). The allelic variants RB1-AA and AG/rs9568036 were determined to be associated with tumor chemosensitivity (p<0.05). A statistically significant relation between the polymorphism RB1-GG/rs9568036 and males (p = 0.0386), rate ratio (RR) = 2.0 (95% confidence interval [CI] = 0.76-5.32), as well as between the allelic variants RB1-AA and AG/rs9568036 and females (p = 0.0027), RR = 8.0 (95% CI = 1.28-50.04), was observed. We also observed a statistically significant association between the rs1042522 polymorphism in the TP53 gene and unilateral presentation of the disease. CONCLUSIONS: The rs9568036 polymorphism in the RB1 gene and the allelic variants can be associated with type of response to medical therapy and associated with male sex, while the allelic variant rs1042522 polymorphism in the TP53 gene is associated with the unilateral presentation of the disease in a group of Mexican children with RB.
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Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único/genética , Proteína do Retinoblastoma/genética , Retinoblastoma/genética , Proteína Supressora de Tumor p53/genética , Criança , Pré-Escolar , DNA de Neoplasias/genética , Frequência do Gene/genética , Humanos , MéxicoRESUMO
Patients with Gaucher type 1 (GD1) throughout Argentina were enrolled in the Argentine bone project to evaluate bone disease and its determinants. We focused on presence and predictors of bone lesions (BL) and their relationship to therapeutic goals (TG) with timing and dose of enzyme replacement therapy (ERT). A total of 124 patients on ERT were enrolled in a multi-center study. All six TG were achieved by 82% of patients: 70.1% for bone pain and 91.1% for bone crisis. However, despite the fact that bone TGs were achieved, residual bone disease was present in 108 patients on ERT (87%) at time 0. 16% of patients showed new irreversible BL (bone infarcts and avascular osteonecrosis) despite ERT, suggesting that they appeared during ERT or were not detected at the moment of diagnosis. We observed 5 prognostic factors that predicted a higher probability of being free of bone disease: optimal ERT compliance; early diagnosis; timely initiation of therapy; ERT initiation dose ≥45 UI/kg/EOW; and the absence of history of splenectomy. Skeletal involvement was classified into 4 major phenotypic groups according to BL: group 1 (12.9%) without BL; group 2 (28.2%) with reversible BL; group 3 (41.9%) with reversible BL and irreversible chronic BL; and group 4 (16.9%) with acute irreversible BL. Our study identifies prognostic factors for achieving best therapeutic outcomes, introduces new risk stratification for patients and suggests the need for a redefinition of bone TG. Am. J. Hematol. 91:E448-E453, 2016. © 2016 Wiley Periodicals, Inc.
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Doenças Ósseas/diagnóstico , Doença de Gaucher/complicações , Adolescente , Adulto , Idoso , Argentina , Doenças Ósseas/etiologia , Doenças Ósseas/patologia , Criança , Diagnóstico Precoce , Terapia de Reposição de Enzimas , Doença de Gaucher/diagnóstico , Doença de Gaucher/tratamento farmacológico , Doença de Gaucher/epidemiologia , Humanos , Adesão à Medicação , Pessoa de Meia-Idade , Fenótipo , Prognóstico , Medição de Risco , Esplenectomia , Adulto Jovem , beta-Glucosidase/uso terapêuticoRESUMO
AIM: To evaluate a predictive model of microalbuminuria by using anthropometric, clinical and genetic variables in relatives of subjects with diabetic nephropathy. METHODS: Eligible subjects, aged 18-63 years with body mass index<35 kg/m2, and first degree relatives of patients with type 2 diabetes and diabetic nephropathy were enrolled in a cross-sectional study. A total of 70 individuals with microalbuminuria were compared with 60 individuals without microalbuminuria. Based on a morning urinary sample, microalbuminuria was defined as albumin≥30<300 mg/dL. Genotyping of single nucleotide polymorphisms (SNPs) G-174C of IL6 (rs1800795), G-308A of TNF (rs1800629), and Pro12Ala of PPARγ2 (rs1801282) genes were determined. The multivariable dimensionality reduction analysis was performed using the software multifactor dimensionality reduction package. RESULTS: The multivariable dimensionality reduction analysis showed that obesity and SNP G-308A of TNF gene exhibited main effects with 1.10 and 1.98% of information gain (IG), respectively. The IL6 showed synergy (interaction) with HDL-c (IG 1.27%) and sex (IG 1.02%); also high-sensitivity C-reactive protein and triglycerides levels showed synergy (IG 1.08%). The consistency of the cross-validation for this model was 0.6836, with sensitivity and specificity of 0.58 and 0.76 (odds ratio 4.64; 95% CI 4.0-10.0, p<0.0001). CONCLUSION: Our results indicate that obesity and/or high blood pressure, in synergism with high-sensitivity C-reactive protein and high-density lipoprotein cholesterol levels, is the main predictive risk factor of diabetic nephropathy in healthy subjects, relatives of patients with type 2 diabetes and diabetic nephropathy.
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Albuminúria/sangue , Nefropatias Diabéticas/sangue , Adolescente , Adulto , Estudos Transversais , Família , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Biológicos , Modelos Estatísticos , Análise Multivariada , Fatores de Risco , Adulto JovemRESUMO
Nonalcoholic fatty liver disease has become the most common liver disorder worldwide, reaching a prevalence of 60% and 24% in patients with chronic liver disease and the general population, respectively. Liver function is often assessed using standard liver tests such as alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, and alkaline phosphatase. Randomized controlled trials (RCTs) investigating the potential beneficial effects of coffee consumption on liver function are scarce and their results are inconclusive. Some clinical trials have shown a significant increase in adiponectin concentrations following coffee consumption; however, there are few studies in this field. Hence, the hypothesis of this meta-analysis of RCTs is that coffee consumption decreases blood markers of liver function and increases adiponectin concentrations. A systematic search was conducted in PubMed-MEDLINE, Scopus, Web of Science, ClinicalTrials.gov, and Google Scholar databases. Meta-analysis was performed using a random-effects model followed by sensitivity analysis. Meta-analysis of 14 RCTs, including a total of 897 subjects, showed that coffee consumption has no significant effect on alanine aminotransferase (weighted mean difference [WMD], -0.89 mg/mL; 95% CI, -2.90 to 1.12; P = .39), aspartate aminotransferase (WMD, -0.29 mg/mL; 95% CI, -1.25 to 0.66; P = .55), gamma-glutamyl transferase (WMD, .10 mg/mL; 95% CI, -3.94 to 4.15; P = .96), alkaline phosphatase (WMD, -4.60 mg/mL; 95% CI, -9.26 to 0.07; P = .05), and lactate dehydrogenase (WMD, -0.65 mg/mL; 95% CI, -10.80 to 9.49; P = .90). However, coffee administration significantly increased adiponectin concentrations (WMD, 1.19 mg/mL; 95% CI, 0.08-2.31; P = .04). The results of this meta-analysis of RCTs suggest that coffee consumption may improve liver dysfunction through the elevation of adiponectin levels; however, further clinical trials are needed to corroborate our findings.
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Adiponectina , Café , Alanina/farmacologia , Alanina Transaminase , Fosfatase Alcalina , Aspartato Aminotransferases , Biomarcadores , Humanos , Lactato Desidrogenases , Fígado , Ensaios Clínicos Controlados Aleatórios como Assunto , gama-GlutamiltransferaseRESUMO
BACKGROUND: It is estimated that up to 90% of head and neck infections have an odontogenic origin, which are considered among the most common in the oral cavity and maxillofacial region. Bacterial resistance has been 1 of the main problems related to the treatment of this type of infection in recent years. The frequency of this resistance is increasing, which is mainly due to patient self-medication and the mutations that bacteria present. Therefore, the objective of this study is to analyze the antimicrobial resistance of antibiotics commonly administered for the treatment of odontogenic infections. METHOD: To carry out the study, PubMed, ScienceDirect, and Scopus databases were reviewed using the keywords "odontogenic infection", "pharmacological treatment", and "microbial resistance. Studies whose main objective was the pharmacological treatment of odontogenic infections were selected. Exclusions were review-type studies, systematic reviews, or in vitro or animal model studies. For the analysis of risk of bias, the Checklist for Analytical Cross-Sectional Studies of the Joanna Briggs Institute was used. The search and analysis of the studies was carried out by 2 researchers independently. RESULTS: A total of 13 studies were included in this review. The mean age was 39.6 years; the location of the infection in the study subjects was in the submandibular and vestibular spaces; there were periodontal, periapical, and dentoalveolar lesions; the main microorganisms identified were Streptococcus, Staphylococcus, Prevotella, Peptostreptococcus, Clostridium, and Klebsiella; and finally, the main microorganisms identified for bacterial resistance were penicillin, clindamycin and amoxicillin. CONCLUSION: The health professional is obliged to update their knowledge to avoid such antibiotic resistance and thus provide better patient care.
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Antibacterianos , Infecções Bacterianas , Humanos , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Estudos Transversais , Farmacorresistência Bacteriana , Infecções Bacterianas/tratamento farmacológico , Amoxicilina/uso terapêutico , Testes de Sensibilidade Microbiana , Literatura de Revisão como AssuntoRESUMO
INTRODUCTION: Periodontal disease (PD) is a chronic inflammation of the soft tissues that support the structure of the tooth, and miRNAs are highly dynamic molecules that participate in the regulation of gene expression interfering with multiple genetic targets. The dysregulation of the expression of miRNAs has been associated with different types of pathologies; therefore, they are excellent molecules to be studied as biomarkers. Material and Methods. A search was made in the electronic databases of PubMed, Scopus, and Science Direct. The following key words were used: "microRNAs," "miRNAs," "periodontal disease," "periodontitis," and "biomarker"; employee independent search strategies with the Boolean operators "OR" and "AND"; a further search of the references of the selected studies was performed to detect potential studies that met the selection criteria. The data recollected from each article were author, country, year of publication, sample size, type of sample used to identify miRNAs, methodology used to identify miRNAs, type of periodontal disease, and miRNAs identified. RESULTS: Of the 13 selected studies, 6 used gingival tissue as a sample for the identification of miRNAs, 3 used gingival fluid, 2 used saliva, 1 used serum, and another used periodontal tissue. Chronic periodontitis was the most studied periodontal disease in 9 of the 13 selected articles; 7 used microarrays as the main technique for the identification of miRNAs. qRT-PCR was the assay choice to validate the identified miRNAs. CONCLUSION: The main type of periodontal disease on which most studies are focused is chronic periodontitis, with the main miRNAs being hsa-miR-146a, hsa-miR-146b, hsa-miR-155, and hsa-miR-200. This systematic review is one of the first to carry out an analysis of the current role of miRNAs in PD as biomarkers.
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MicroRNAs/biossíntese , Doenças Periodontais/genética , Animais , Biologia Computacional/métodos , Humanos , MicroRNAs/genética , Doenças Periodontais/metabolismo , Doenças Periodontais/patologiaRESUMO
BACKGROUND: Oral lichen planus is a chronic inflammatory skin disease of autoimmune nature, with infiltration of T lymphocytes that destroy the basal stratum, which causes white striae, erosions, ulcers and breaks in epithelial continuity. CASE REPORT: 54-year-old woman with multiple reticular and erosive lesions in the region of the buccal mucosa and lateral borders of the tongue, as well as desquamative gingivitis. Treatment with mometasone was started thrice daily for 20 days and intermittently with miconazole gel to prevent iatrogenic mycosis by the corticosteroid. The patient was examined at 20 days, after which 80 % improvement of the lesions that had central ulcerations was observed, which allowed the ingestion of food and fluids without pain and improved the quality of life. CONCLUSIONS: In autoimmune diseases, clinical-histopathological correlation is important for a definitive diagnosis to be established. Clinical follow-up of these patients should be carried out in the long term, in order to achieve remission of the lesions, control of the disease and avoid future complications.
Antecedentes: El liquen plano bucal es una enfermedad dermatológica inflamatoria crónica de base autoinmune, con infiltración de linfocitos T que destruyen el estrato basal, lo que ocasiona estrías blancas, erosiones, úlceras y diluciones epiteliales de continuidad. Reporte de caso: Mujer de 54 años con múltiples lesiones reticulares y erosivas en región de la mucosa yugal y bordes laterales de la lengua, así como gingivitis descamativa. Se inició tratamiento con mometasona tres veces al día por 20 días e intermitentemente con miconazol en gel, para prevenir micosis iatrogénica por el corticosteroide. La paciente fue revisada a los 20 días, al cabo de los cuales se observó mejoría de 80 % de las lesiones con ulceraciones centrales, lo que permitió la ingesta de alimentos y líquidos sin dolor y mejoró la calidad de vida. Conclusiones: En las enfermedades autoinmunes es importante la correlación clínico-histopatológica para establecer el diagnóstico definitivo. El seguimiento clínico de estos pacientes se debe realizar a largo plazo, para lograr la remisión de las lesiones, control de la enfermedad y evitar futuras complicaciones.
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Líquen Plano Bucal , Feminino , Humanos , Líquen Plano Bucal/diagnóstico , Líquen Plano Bucal/tratamento farmacológico , Pessoa de Meia-IdadeAssuntos
Alanina Transaminase/sangue , Glicemia/análise , Diabetes Mellitus Tipo 2/enzimologia , Jejum/sangue , Hepatopatias/enzimologia , Obesidade/enzimologia , Adulto , Idoso , Biomarcadores/sangue , Índice de Massa Corporal , Estudos de Casos e Controles , Estudos Transversais , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/diagnóstico , Feminino , Humanos , Hepatopatias/sangue , Hepatopatias/diagnóstico , Modelos Logísticos , Pessoa de Meia-Idade , Obesidade/sangue , Obesidade/diagnóstico , Razão de Chances , Regulação para CimaRESUMO
Introducción: la agenesia dental no sindrómica (ADNS) genera efec- tos negativos en la salud oral y psicosocial de los seres humanos. El determinante genético desempeña un papel importante en su desarrollo. Objetivo: determinar la frecuencia de los polimorfismos rs104893850 de MSX1 y rs28933373 de PAX9 en pacientes de seis a 18 años con ADNS. Material y métodos: estudio transversal prolectivo en el cual se revisaron individuos de seis a 18 años sin defectos congénitos y originarios del estado de Durango. Después de haber obtenido su con- sentimiento para formar parte del estudio, se estableció el diagnóstico de ADNS a través de una inspección clínica odontológica y un examen radiográfico. Se tomó una muestra de sangre capilar para la genotipi- ficación de los polimorfismos a través de la técnica de qPCR-HRM. Resultados: de un total de 124 individuos, 77 (62%) mujeres y 47 (38%) hombres; sólo 39 presentaron ADNS. En el análisis polimórfico de rs104893850 de MSX1 y rs28933373 de PAX9 se obtuvo 94.9% y 84.6% respectivamente de homocigotos mutados. Conclusiones: se obtuvo una alta frecuencia de hipodoncia, el diente que mostró más agenesia fue el órgano dentario 18. Las mutaciones polimórficas están presentes en una alta proporción de agenesia dental (AU)
Introduction: non-syndromic dental agenesis (NSDA) generates negative oral health and psychosocial effects in humans. The genetic determinant plays an important role in its development. Objective: to determine the frequency of MSX1 rs104893850 and PAX9 rs28933373 polymorphisms in patients aged 6 to 18 years with NSDA. Material and methods: prolective cross-sectional study, in which individuals aged 6 to 18 years without congenital defects and from the city of Durango were reviewed. After obtaining their consent to be part of the study, the diagnosis of NSDA was established through a clinical dental inspection, a radiographic examination and a capillary blood sample was taken for the genotyping of the polymorphisms through the qPCR-HRM technique. Results: out of a total of 124 individuals, 77 (62%) females and 47 (38%) males; only 39 presented ADNS. In the polymorphic analysis of rs104893850 of MSX1 and rs28933373 of PAX9 we obtained 94.9% and 84.6% respectively of mutated homozygotes. Conclusions: a high frequency of hypodontia was obtained, and the tooth that presented the most agenesis was dental organ 18. Polymorphic mutations are present in a high proportion for dental agenesis (AU)
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Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Polimorfismo Genético , Anormalidades Dentárias/genética , Anodontia/genética , Odontogênese/genética , Faculdades de Odontologia , Reação em Cadeia da Polimerase/métodos , Epidemiologia Descritiva , Estudos Transversais , Anodontia/diagnóstico por imagem , MéxicoRESUMO
La enfermedad por coronavirus 2019 (COVID-19) es una infección respiratoria aguda altamente transmisible, la cual ha sido declarada pandemia por la Organización Mundial de la Salud. En concordancia con las políticas gubernamentales para la prevención sanitaria y aislamiento social, se aprobó la virtualidad de todo el sistema de educación, incluyendo carreras con un fuerte componente práctico como las de Ciencias de la Salud; lo cual conllevo a la adaptación de planes y programas de estudio, así como a la inmersión de utilización de plataformas virtuales, generando un descubrimiento de nuevas formas de aprendizaje tanto en el área teórica y práctica, sin embargo al mismo tiempo se generaron ciertas repercusiones en destrezas y habilidades de tipo prácticas; abriendo además, una brecha entre la desigualdad en el acceso de las tecnologías.
Coronavirus disease 2019 (COVID-19) is a highly communicable acute respiratory infection, which has been declared a pandemic by the World Health Organization. In accordance with government policies for health prevention and social isolation, the virtuality of the entire education system was approved, including careers with a strong practical component such as health sciences; which led to the adaptation of study plans and programs, as well as the immersion of the use of virtual platforms, generating a discovery of new forms of learning both in the theoretical and practical areas, however, at the same time, certain repercussions were generated in practical and psychomotor skills and abilities; also opening a gap between inequality in access to technologies.
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BACKGROUND: Results of previous clinical trials evaluating the effect of magnesium supplementation on inflammatory markers are controversial. OBJECTIVE: A systematic review and meta-analysis of randomized controlled trials (RCTs) were performed to evaluating the effect of oral magnesium supplementation on plasma C-reactive protein (CRP) concentrations. METHOD: PubMed-Medline, SCOPUS, Web of Science and Google Scholar databases were searched (from inception to August 09, 2016) to identify RCTs, evaluating the effect of magnesium on CRP levels. A random-effects model and a generic inverse variance method were used to compensate for the heterogeneity of studies. Publication bias, sensitivity analysis, and meta-regression assessments were conducted using standard methods. RESULTS: Overall, the impact of magnesium supplementation on plasma concentrations of CRP was assessed in 11 studies. Magnesium treatment was not found to significantly affect plasma concentrations of CRP (WMD: -0.11 mg/L, 95% CI: -0.75, 0.52, p=0.727). When the analysis was stratified to compare subgroups of studies in populations with baseline plasma CRP values of ≤ 3 and > 3 mg/L, a significant reduction of CRP values was observed in the latter subgroup (WMD: -1.12 mg/L, 95% CI: -2.05, -0.18, p=0.019) but not in the former group (WMD: 0.61 mg/L, 95% CI: -0.10, 1.32, p=0.090). The difference between subgroups was statistically significant (p=0.004). CONCLUSION: Results of the present meta-analysis indicated that magnesium supplementation reduces CRP levels among individuals with inflammation (CRP levels > 3 mg/dL). This finding suggests that magnesium supplements may have a beneficial role as an adjuvant for the management of low-grade chronic systemic inflammation.
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Proteína C-Reativa/metabolismo , Suplementos Nutricionais , Inflamação/sangue , Magnésio/farmacologia , Proteína C-Reativa/análise , Humanos , Inflamação/tratamento farmacológico , Magnésio/administração & dosagem , Magnésio/uso terapêutico , Ensaios Clínicos Controlados Aleatórios como AssuntoRESUMO
Objetivo. Reportar el perfil epidemiológico y presencia de polimorfismos de IFR6 (rs2235371) y TGFA (rs3771494) en individuos con labio y paladar hendido (LPH) no sindrómico. Métodos. Serie de casos, centrado en individuos con hendiduras orofaciales (HO), remitidos a la Facultad de Odontología de Durango, México, para su revisión y valoración dentro del periodo comprendido de enero-2018 a diciembre-2019. Se obtuvo información de interés para el estudio sobre las características del embarazo y anteceden- tes familiares, de las madres de los individuos. Para el análisis y descripción de los datos se utilizó el paquete estadístico R Studio. Resultados. El total de casos estudiados fue de 24. Se presentó una mayor frecuencia de hombres (67%), la mayoría de los individuos tuvieron el diagnóstico de LPH al nacer (75%), extensión de afección completa (75%), el lado izquierdo afectado (63%), y cirugías correctivas (62%). Solo el 4% estuvo ex- puesto al tabaquismo materno (activo y pasivo), el 96% tuvo adecuada ingesta de ácido fólico y hierro; el genotipo de riesgo para TGFA (rs3771494) se presentó en el 15% de labio hendido y en el 8% del LPH, y para IFR6 (rs2235371) solo en el 10% del LPH. Conclusiones. La presencia de factores ambientales, genéticos, y el estilo de vida ma- terno mostrado en otras poblaciones, podrían no ser los mismos que intervengan en la aparición y desarrollo de HO no sindromicas de nuestra localidad, además, la presencia de los genotipos homocigotos polimórficos de los genes de interés podría no condicionar el desarrollo de HO.
Objective. To report the epidemiological profile and the presence of polymorphisms of the IFR6 (rs2235371) and TGFA (rs3771494) genes, in individuals with non-syndromic cleft lip and palate. Methods. Case series study, focused on individuals with orofacial clefts, referred to the School of Dentistry of Durango, Mexico, for review and assessment within the period from January 2018 to December 2019. Information of interest for the study was obtained on the characteristics of pregnancy and family history of the mothers of the individuals. For the analysis and description of the data, the statistical software R Studio was used. Results. There was a higher frequency of men (67%), most of the individuals had the diagnosis of cleft lip and palate at birth (75%), the extension of the complete affection (75%), the left side affected (63%), and corrective surgeries (62%). Only 4% were exposed to maternal smoking (active and passive), 96% had adequate folic acid and iron intake; the risk genotype for TGFA (rs3771494 [G/G]) was present in 15% of cleft lip and 8% of cleft lip and palate, and for IFR6 (rs2235371 [T/T]) only in 10% of cleft lip and palate. Conclusions. The presence of environmental and genetic factors, as well as the maternal lifestyle shown in other populations, may not be the same as those involved in the appearance and development of non-syndromic orofacial clefts in our locality, in addition, the presence of homozygous polymorphic genotypes of the genes of interest could not condition the development of orofacial clefts.
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RESUMEN: Tener una clasificación de enfermedades periodontales es de gran utilidad para los clínicos al brindar una guía sistematizada de las distintas manifestaciones de los problemas periodontales. La nueva clasificación de enfermedades y condiciones periodontales de 2017 tiene como una de sus características principales la agrupación de la periodontitis con estadios y grados, como manifestación de enfermedades sistémicas y enfermedades periodontales necrosantes; así como la introducción de un apartado que habla de salud y enfermedad peri-implantaria. En el caso de la periodontitis, el estadio se refiere a la severidad de la enfermedad y la complejidad del tratamiento; mientras que el grado se refiere al ritmo del progreso y riesgo de recidiva de la enfermedad, en este trabajo se ofrece una ruta de diagnóstico didáctica y se desarrollan las principales diferencias entre las últimas dos clasificaciones, al tiempo que compartimos la experiencia al implementar esta nueva clasificación en nuestro instituto.
ABSTRACT: Having a classification of periodontal conditions and diseases is a very useful tool for clinicians by providing a systematic guide to different manifestations of periodontal issues. The 2017 classification of periodontal diseases and conditions has as one of the main characteristics the division of the Periodontitis group classified by stage, which assigns the severity and complexity of the treatment, and by grade, which refers to the rhythm of progression and the risk of future relapse; periodontitis as a manifestation of systemic conditions and necrotizing periodontal diseases. As well as the featuring of peri-implant health and diseases. A more didactic route of diagnostic will be given in this publication and the main differences between the last two classifications will be explained, while we share the experience of implementing this new classification in our institute.
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Peripheral giant cell granuloma (PGCG) is a relatively common benign reactive lesion of the oral cavity which can occur at any age. CTCFL/BORIS (CTCF like/Brother of the Regulator of Imprinted Sites) and CTCF (CCCTC-binding factor) are paralogous genes with an important role in the regulation of gene expression, genomic imprinting, and nuclear chromatin insulators regulation. BORIS expression promotes cell immortalization and growth while CTCF has tumor suppressor activity; the expression pattern may reflect the reverse transcription silencing of BORIS. The aim of this work was to describe a histopathological and molecular approach of an 8-year-old pediatric male patient with PGCG diagnosis. It was observed that the PGCG under study expressed CTCF as well as BORIS mRNAs alongside with the housekeeping gene GAPDH, which may be related to possible genetic and epigenetic changes in normal cells of oral cavity.
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RESUMEN: La hiperplasia condilar (HC) es un término genérico de una condición patológica que se utiliza para describir situaciones que causan el crecimiento excesivo y sobredesarrollo del cóndilo mandibular, repercutiendo así también en la mandíbula, es la responsable de alrededor del 50 % de las asimetrías faciales y se presenta con mayor frecuencia entre los 11 y 30 años de edad. Se presenta un caso clínico de una paciente femenina de 21 años de edad que presentaba hiperplasia condilar unilateral izquierda con compensación alveolodentaria. Se realizó condilectomía alta con abordaje endoaural para retirar 5 mm de la parte superior del cóndilo y osteotomía mandibular mediante abordaje intraoral circunvestibular, con disección y preservación del nervio dentario inferior, retirando 8 mm del aspecto inferior de la mandíbula de acuerdo a los requerimientos estéticos. El brindar un tratamiento adecuado a la hiperplasia condilar enfocado a corregir las secuelas tanto funcionales como estéticas es de gran beneficio al paciente ya que le permite mejorar su calidad de vida, el correcto diagnóstico es vital para poder planear un tratamiento exitoso.
ABSTRACT: Condylar hyperplasia (CH) is a generic term for a pathological situation that is used to describe conditions that cause excessive growth and overdevelopment of the mandibular condyle and also impacting on the jaw, this bone formation is responsible about of 50 % of all deformities facial and it occurs most frequently between 11-30 years old. This case report is about a 21-years old female who showed unilateral condylar hyperplasia of left side with alveolodentary compensatory. Was carried out high condilectomy through an endoaural approach removing 5 mm of the upper condyle and the mandibular osteotomy was performed through intraoral approach with dissection and preservation of the inferior alveolar nerve, removing 8 mm of the inferior aspect of the mandible according to the aesthetic requirements. An appropriate treatment for the condylar hyperplasia focused on correcting the side effects such as functional or aesthetic it's of great benefit for the patient because it allows improve their quality of life. A correct diagnosis is very important to planning a successful treatment.
Assuntos
Humanos , Feminino , Adulto Jovem , Doenças Mandibulares/cirurgia , Côndilo Mandibular/cirurgia , Radiografia Panorâmica , Doenças Mandibulares/patologia , Doenças Mandibulares/diagnóstico por imagem , Estética Dentária , Assimetria Facial/cirurgia , Procedimentos Cirúrgicos Ortognáticos/métodos , Hiperplasia , Côndilo Mandibular/patologia , Côndilo Mandibular/diagnóstico por imagemRESUMO
The aim of this work was to analyze methylation of the promoter sites of the ESR1 and PGR genes and to determine correlations with immunohistochemical expression of estrogen and progesterone receptors in ductal and lobular breast cancers. An observational, descriptive, molecular study was conducted on 20 ductal and 20 lobular breast cancer samples with immunohistochemical determination of estrogen and progesterone receptor expression. The methylation analysis of ESR1 and PGR promoter sites was carried-out by methylation-specific PCR. For correlation analysis, Kendall's tau coefficient was determined. Positive correlations were found between estrogen and progesterone receptors, estrogen receptor and unmethylated progesterone receptor, progesterone receptor, and unmethylated progesterone receptor. Negative correlations were found between estrogen receptor and methylated progesterone receptor, progesterone receptor and methylated progesterone receptor, methylated and unmethylated estrogen receptor, and methylated and unmethylated progesterone receptor. The results suggest that methylation of promoter sites of ESR1 and PGR is a relatively uncommon event in ductal and lobular breast cancer, and also suggest that the determination of epigenetic states of ESR1 and PGR could represent an alternative or complement to the histopathological expression analysis.
Assuntos
Carcinoma Ductal de Mama/genética , Carcinoma Lobular/genética , Metilação de DNA/genética , Receptor alfa de Estrogênio/genética , Peptídeos e Proteínas de Sinalização Intracelular/genética , Proteínas Nucleares/genética , Idoso , Biomarcadores Tumorais/genética , Feminino , Humanos , Pessoa de Meia-Idade , Regiões Promotoras Genéticas , Receptores de Estrogênio/genética , Receptores de Progesterona/genéticaRESUMO
Chronic systemic inflammation, characterized by elevated levels of the acute phase proteins, such as C-reactive protein (CRP), plays an important role in the pathogenesis of glucose metabolic disturbances and diabetes. The aim of this study was to determine if the elevated levels of CRP are associated with impaired fasting glucose (IFG) in obese subjects. Healthy obese men and nonpregnant obese women were enrolled in a case-control study. Individuals with new diagnosis of IFG were considered as cases and compared with a control group without IFG. Elevated CRP was defined by high-sensitivity C-reactive protein (hsCRP) levels between 3.0 and 10.0 mg/L and new diagnosis of IFG by the presence of fasting plasma glucose levels of 100 to 126 mg/dL. A total of 74 subjects were allocated into the case group and compared with 74 subjects in the control group. Elevated hsCRP was identified in 61 (41.2 %) and 34 (23.0 %) individuals in the case and control groups, respectively (p < 0.001). The adjusted odds ratio used to assess the association between elevated hsCRP levels and IFG was 3.36; 95 % confidence interval was 1.66-6.79. In conclusion, the elevated hsCRP levels are associated with IFG in obese subjects.
Assuntos
Glicemia/análise , Proteína C-Reativa/análise , Glucose/metabolismo , Obesidade/metabolismo , Adulto , Estudos de Casos e Controles , Jejum/sangue , Feminino , Teste de Tolerância a Glucose , Humanos , Interleucina-6/sangue , Masculino , Fator de Necrose Tumoral alfa/sangueRESUMO
A growing body of evidence from experimental studies that shows the essential role that magnesium exerts on glucose metabolism has been developed in last years, strongly suggesting that magnesium could plays an important roles in the reduction of the risk of developing type 2 diabetes. In the clinical setting, large epidemiological studies show that low dietary magnesium intake is associated with the increased risk of developing type 2 diabetes; however, results from randomized controlled clinical trials that have evaluated the beneficial effects of magnesium supplementation on glucose metabolism and insulin sensitivity are controversial. In this article we searched (in the electronic databases of Medline, Embase, and the Cochrane Controlled Trials Register up to June 2011) the evidence derived from epidemiological studies and clinical trials, about the relationship between magnesium and type 2 diabetes. The body of evidence from epidemiological studies consistently shows a strong inverse relationship between dietary magnesium intake and the risk of developing T2D; however, results from clinical trials are scarce and controversial.
Assuntos
Diabetes Mellitus Tipo 2/etiologia , Magnésio/fisiologia , Diabetes Mellitus Tipo 2/dietoterapia , Suplementos Nutricionais , Estudos Epidemiológicos , Humanos , Magnésio/administração & dosagem , Magnésio/metabolismo , Deficiência de Magnésio/complicações , Ensaios Clínicos Controlados Aleatórios como Assunto , Estados Unidos/epidemiologiaRESUMO
BACKGROUND AND AIMS: There are no studies that elucidate whether the role of inflammation in the increase of urinary albumin is independent, mediated by family history or by risk factors acquired during life in the offspring of subjects with type 2 diabetes. We undertook this study to evaluate whether elevated C-reactive protein (CRP) levels are independently associated with urinary albumin-to-creatinine ratio (UACR) in the offspring of subjects with diabetic nephropathy. METHODS: A total of 64 healthy males and healthy nonpregnant females, offspring of subjects with diabetic nephropathy, aged 18-69 years, and with body mass index ≤35 kg/m(2) were enrolled in a cross-sectional study. Hypertension, glucose metabolic disorders, metabolic syndrome, smoking, alcohol intake, chronic or acute infections, renal disease, neoplasm, cardiovascular disease, degenerative disease, intake of anti-inflammatory drugs, exercise, or sexual intercourse in the previous 72 h were exclusion criteria. Subjects with high-sensitivity CRP (hsCRP) levels ≥3.0 mg/dL were compared with a gender- and age-matched control group of subjects with hsCRP levels <3.0 mg/dL. RESULTS: The multivariate linear regression analysis showed that hsCRP (B = 0.50, ß = 0.583, p = 0.02), total body fat (B = -2.80, ß = 0.473, p = 0.03), BMI (B = -1.45, ß = 0.390, p = 0.04) and waist circumference (B = 0.89, ß = 0.407, p = 0.04) are predictors for elevation of UACR (Table 2). However, in the stepwise model only hsCRP (B = 0.674; ß = 0.314; p = 0.04) remained significantly associated with UACR. CONCLUSIONS: Our results show that independent of the primary risk factors, elevated hsCRP levels are associated with UACR.