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1.
Bioinformatics ; 26(15): 1819-26, 2010 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-20519285

RESUMO

MOTIVATION: New sequencing technologies have accelerated research on prokaryotic genomes and have made genome sequencing operations outside major genome sequencing centers routine. However, no off-the-shelf solution exists for the combined assembly, gene prediction, genome annotation and data presentation necessary to interpret sequencing data. The resulting requirement to invest significant resources into custom informatics support for genome sequencing projects remains a major impediment to the accessibility of high-throughput sequence data. RESULTS: We present a self-contained, automated high-throughput open source genome sequencing and computational genomics pipeline suitable for prokaryotic sequencing projects. The pipeline has been used at the Georgia Institute of Technology and the Centers for Disease Control and Prevention for the analysis of Neisseria meningitidis and Bordetella bronchiseptica genomes. The pipeline is capable of enhanced or manually assisted reference-based assembly using multiple assemblers and modes; gene predictor combining; and functional annotation of genes and gene products. Because every component of the pipeline is executed on a local machine with no need to access resources over the Internet, the pipeline is suitable for projects of a sensitive nature. Annotation of virulence-related features makes the pipeline particularly useful for projects working with pathogenic prokaryotes. AVAILABILITY AND IMPLEMENTATION: The pipeline is licensed under the open-source GNU General Public License and available at the Georgia Tech Neisseria Base (http://nbase.biology.gatech.edu/). The pipeline is implemented with a combination of Perl, Bourne Shell and MySQL and is compatible with Linux and other Unix systems.


Assuntos
Genoma Bacteriano/genética , Genômica/métodos , Células Procarióticas , Bordetella bronchiseptica/genética , Georgia , Neisseria meningitidis/genética , Análise de Sequência de DNA/métodos , Software
2.
J Bacteriol ; 192(4): 1030-44, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20008069

RESUMO

Legionella longbeachae causes most cases of legionellosis in Australia and may be underreported worldwide due to the lack of L. longbeachae-specific diagnostic tests. L. longbeachae displays distinctive differences in intracellular trafficking, caspase 1 activation, and infection in mouse models compared to Legionella pneumophila, yet these two species have indistinguishable clinical presentations in humans. Unlike other legionellae, which inhabit freshwater systems, L. longbeachae is found predominantly in moist soil. In this study, we sequenced and annotated the genome of an L. longbeachae clinical isolate from Oregon, isolate D-4968, and compared it to the previously published genomes of L. pneumophila. The results revealed that the D-4968 genome is larger than the L. pneumophila genome and has a gene order that is different from that of the L. pneumophila genome. Genes encoding structural components of type II, type IV Lvh, and type IV Icm/Dot secretion systems are conserved. In contrast, only 42/140 homologs of genes encoding L. pneumophila Icm/Dot substrates have been found in the D-4968 genome. L. longbeachae encodes numerous proteins with eukaryotic motifs and eukaryote-like proteins unique to this species, including 16 ankyrin repeat-containing proteins and a novel U-box protein. We predict that these proteins are secreted by the L. longbeachae Icm/Dot secretion system. In contrast to the L. pneumophila genome, the L. longbeachae D-4968 genome does not contain flagellar biosynthesis genes, yet it contains a chemotaxis operon. The lack of a flagellum explains the failure of L. longbeachae to activate caspase 1 and trigger pyroptosis in murine macrophages. These unique features of L. longbeachae may reflect adaptation of this species to life in soil.


Assuntos
Proteínas de Bactérias/genética , Genoma Bacteriano , Legionella longbeachae/genética , Legionella longbeachae/patogenicidade , Legionelose/microbiologia , Análise de Sequência de DNA , Fatores de Virulência/genética , Idoso , Sequência Conservada , Feminino , Humanos , Legionella longbeachae/isolamento & purificação , Legionella pneumophila/genética , Dados de Sequência Molecular , Oregon , Sintenia
3.
J Virol ; 81(23): 12758-65, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17898056

RESUMO

Whole genome phylogenetic analysis in this study resolved a total of five major genotypes among the 22 varicella-zoster virus (VZV) strains or isolates for which complete genomic sequences are available. Consistent with earlier publications we have designated these genotypes European 1 (E1), European 2 (E2), Japanese (J), mosaic 1 (M1), and mosaic 2 (M2). Single nucleotide polymorphism (SNP) analysis performed in a whole-genome alignment revealed that VZV isolates of all five genotypes can be accurately genotyped using SNPs from two amplicons: open reading frame 22 (ORF22) and either ORF21 or ORF50. This modified approach identifies all of the genotypes observed using any of the published genotyping protocols. Of 165 clinical varicella and zoster isolates from Australia and New Zealand typed using this approach, 67 of 127 eastern Australian isolates were E1, 30 were E2, 16 were J, 10 were M1, and 4 were M2; 25 of 38 New Zealand isolates were E1, 8 were E2, and 5 were M1. VZV strain diversity in eastern Australia is thus broader than has been described for any other region, including Europe, Africa, and North America. J strains were far more prevalent than previously observed in countries other than Japan. Two-amplicon typing was in complete accord with genotypes derived using SNP in multiple ORFs (ORFs 1, 21, 22, 38, 50, 54, and 62). Two additional minor genotypes, M3 and M4, could also be resolved using two-amplicon typing.


Assuntos
Técnicas Genéticas , Herpesvirus Humano 3/classificação , Herpesvirus Humano 3/genética , Polimorfismo de Nucleotídeo Único , Austrália , DNA Viral/genética , Genoma Viral/genética , Genótipo , Herpes Zoster/virologia , Herpesvirus Humano 3/isolamento & purificação , Humanos , Epidemiologia Molecular , Nova Zelândia , Fases de Leitura Aberta , Filogenia , Sensibilidade e Especificidade , Homologia de Sequência
4.
Viruses ; 8(12)2016 12 10.
Artigo em Inglês | MEDLINE | ID: mdl-27973399

RESUMO

Vaccinia virus (VACV) has been implicated in infections of dairy cattle and humans, and outbreaks have substantially impacted local economies and public health in Brazil. During a 2005 outbreak, a VACV strain designated Serro 2 virus (S2V) was collected from a 30-year old male milker. Our aim was to phenotypically and genetically characterize this VACV Brazilian isolate. S2V produced small round plaques without associated comets when grown in BSC40 cells. Furthermore, S2V was less virulent than the prototype strain VACV-Western Reserve (WR) in a murine model of intradermal infection, producing a tiny lesion with virtually no surrounding inflammation. The genome of S2V was sequenced by primer walking. The coding region spans 184,572 bp and contains 211 predicted genes. Mutations in envelope genes specifically associated with small plaque phenotypes were not found in S2V; however, other alterations in amino acid sequences within these genes were identified. In addition, some immunomodulatory genes were truncated in S2V. Phylogenetic analysis using immune regulatory-related genes, besides the hemagglutinin gene, segregated the Brazilian viruses into two clusters, grouping the S2V into Brazilian VACV group 1. S2V is the first naturally-circulating human-associated VACV, with a low passage history, to be extensively genetically and phenotypically characterized.


Assuntos
Genoma Viral , Filogenia , Análise de Sequência de DNA , Vaccinia virus/genética , Vaccinia virus/isolamento & purificação , Vacínia/virologia , Adulto , Animais , Brasil , Linhagem Celular , Modelos Animais de Doenças , Genes Virais , Humanos , Masculino , Camundongos , Homologia de Sequência , Vacínia/patologia , Vaccinia virus/classificação , Vaccinia virus/fisiologia , Ensaio de Placa Viral , Virulência , Fatores de Virulência/genética
5.
PLoS One ; 6(8): e23086, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21858000

RESUMO

Cowpox virus (CPXV) is described as the source of the first vaccine used to prevent the onset and spread of an infectious disease. It is one of the earliest described members of the genus Orthopoxvirus, which includes the viruses that cause smallpox and monkeypox in humans. Both the historic and current literature describe "cowpox" as a disease with a single etiologic agent. Genotypic data presented herein indicate that CPXV is not a single species, but a composite of several (up to 5) species that can infect cows, humans, and other animals. The practice of naming agents after the host in which the resultant disease manifests obfuscates the true taxonomic relationships of "cowpox" isolates. These data support the elevation of as many as four new species within the traditional "cowpox" group and suggest that both wild and modern vaccine strains of Vaccinia virus are most closely related to CPXV of continental Europe rather than the United Kingdom, the homeland of the vaccine.


Assuntos
Vírus da Varíola Bovina/classificação , Vírus da Varíola Bovina/genética , Genoma Viral/genética , Filogenia , Animais , Análise por Conglomerados , Vírus da Varíola Bovina/isolamento & purificação , DNA Viral/química , DNA Viral/genética , Humanos , Dados de Sequência Molecular , Análise de Sequência de DNA , Especificidade da Espécie , Proteínas Virais/genética
6.
PLoS One ; 4(10): e7666, 2009 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-19865479

RESUMO

The data presented herein support the North American orthopoxviruses (NA OPXV) in a sister relationship to all other currently described Orthopoxvirus (OPXV) species. This phylogenetic analysis reaffirms the identification of the NA OPXV as close relatives of "Old World" (Eurasian and African) OPXV and presents high support for deeper nodes within the Chordopoxvirinae family. The natural reservoir host(s) for many of the described OPXV species remains unknown although a clear virus-host association exists between the genus OPXV and several mammalian taxa. The hypothesized host associations and the deep divergence of the OPXV/NA OPXV clades depicted in this study may reflect the divergence patterns of the mammalian faunas of the Old and New World and reflect a more ancient presence of OPXV on what are now the American continents. Genes from the central region of the poxvirus genome are generally more conserved than genes from either end of the linear genome due to functional constraints imposed on viral replication abilities. The relatively slower evolution of these genes may more accurately reflect the deeper history among the poxvirus group, allowing for robust placement of the NA OPXV within Chordopoxvirinae. Sequence data for nine genes were compiled from three NA OPXV strains plus an additional 50 genomes collected from Genbank. The current, gene sequence based phylogenetic analysis reaffirms the identification of the NA OPXV as the nearest relatives of "Old World" OPXV and presents high support for deeper nodes within the Chordopoxvirinae family. Additionally, the substantial genetic distances that separate the currently described NA OPXV species indicate that it is likely that many more undescribed OPXV/NA OPXV species may be circulating among wild animals in North America.


Assuntos
Orthopoxvirus/classificação , Orthopoxvirus/genética , DNA Mitocondrial/genética , DNA Viral/genética , Bases de Dados Factuais , Ecologia , Ecossistema , Evolução Molecular , Especiação Genética , Geografia , América do Norte , Filogenia , Análise de Sequência de DNA , Especificidade da Espécie
7.
Science ; 313(5788): 807-12, 2006 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-16873609

RESUMO

Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.


Assuntos
DNA Viral/genética , Evolução Molecular , Variação Genética , Genoma Viral , Varíola/virologia , Vírus da Varíola/genética , Surtos de Doenças , Deleção de Genes , Genômica , Humanos , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Proteoma/análise , Proteoma/genética , Recombinação Genética , Análise de Sequência de DNA , Varíola/epidemiologia , Varíola/mortalidade , Vírus da Varíola/classificação , Vírus da Varíola/isolamento & purificação , Vírus da Varíola/patogenicidade , Proteínas Virais/química , Proteínas Virais/genética , Virulência/genética
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