RESUMO
BACKGROUND: Salmonella can invade host cells via a type three secretion system called T3SS-1 and its outer membrane proteins, PagN and Rck. However, the mechanism of PagN-dependent invasion pathway used by Salmonella enterica, subspecies enterica serovar Typhimurium remains unclear. RESULTS: Here, we report that PagN is well conserved and widely distributed among the different species and subspecies of Salmonella. We showed that PagN of S. Typhimurium was sufficient and necessary to enable non-invasive E. coli over-expressing PagN and PagN-coated beads to bind to and invade different non-phagocytic cells. According to the literature, PagN is likely to interact with heparan sulfate proteoglycan (HSPG) as PagN-mediated invasion could be inhibited by heparin treatment in a dose-dependent manner. This report shows that this interaction is not sufficient to allow the internalization mechanism. Investigation of the role of ß1 integrin as co-receptor showed that mouse embryo fibroblasts genetically deficient in ß1 integrin were less permissive to PagN-mediated internalization. Moreover, PagN-mediated internalization was fully inhibited in glycosylation-deficient pgsA-745 cells treated with anti-ß1 integrin antibody, supporting the hypothesis that ß1 integrin and HSPG cooperate to induce the PagN-mediated internalization mechanism. In addition, use of specific inhibitors and expression of dominant-negative derivatives demonstrated that tyrosine phosphorylation and class I phosphatidylinositol 3-kinase were crucial to trigger PagN-dependent internalization, as for the Rck internalization mechanism. Finally, scanning electron microscopy with infected cells showed microvillus-like extensions characteristic of Zipper-like structure, engulfing PagN-coated beads and E. coli expressing PagN, as observed during Rck-mediated internalization. CONCLUSIONS: Our results supply new comprehensions into T3SS-1-independent invasion mechanisms of S. Typhimurium and highly indicate that PagN induces a phosphatidylinositol 3-kinase signaling pathway, leading to a Zipper-like entry mechanism as the Salmonella outer membrane protein Rck.
Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Infecções por Salmonella/microbiologia , Salmonella typhimurium/metabolismo , Animais , Proteínas da Membrana Bacteriana Externa/genética , Linhagem Celular , Fibroblastos/metabolismo , Fibroblastos/microbiologia , Humanos , Integrina beta1/genética , Integrina beta1/metabolismo , Camundongos , Infecções por Salmonella/genética , Infecções por Salmonella/metabolismo , Salmonella typhimurium/genética , Sistemas de Secreção Tipo III/genética , Sistemas de Secreção Tipo III/metabolismoRESUMO
One important step for the pathogenesis of Salmonella is its ability to penetrate host cells. Recently, a new entry system involving the outer membrane protein Rck has been characterized. Previous studies have shown that the pefI-srgC locus, which contains rck, was regulated by the temperature and SdiA, the transcriptional regulator of quorum sensing in Salmonella. To decipher the regulation of rck by SdiA, we first confirmed the operon organization of the pefI-srgC locus. Using plasmid-based transcriptional fusions, we showed that only the predicted distal promoter upstream of pefI, PefIP2, displays an SdiA- and acyl-homoserine lactones-dependent activity while the predicted proximal PefIP1 promoter exhibits a very low activity independent on SdiA in our culture conditions. A direct and specific interaction of SdiA with this PefIP2 region was identified using electrophoretic mobility shift assays and surface plasmon resonance studies. We also observed that Rck expression is negatively regulated by the nucleoid-associated H-NS protein at both 25°C and 37°C. This work is the first demonstration of a direct regulation of genes by SdiA in Salmonella and will help further studies designed to identify environmental conditions required for Rck expression and consequently contribute to better characterize the role of this invasin in vivo.
Assuntos
Proteínas de Bactérias/metabolismo , Regulação Bacteriana da Expressão Gênica , Óperon , Salmonella typhimurium/genética , Transativadores/metabolismo , Fatores de Virulência/biossíntese , Fusão Gênica Artificial , DNA Bacteriano/metabolismo , Proteínas de Ligação a DNA/metabolismo , Ensaio de Desvio de Mobilidade Eletroforética , Perfilação da Expressão Gênica , Ordem dos Genes , Genes Bacterianos , Genes Reporter , Regiões Promotoras Genéticas , Ligação Proteica , Percepção de Quorum , Salmonella typhimurium/fisiologia , Ressonância de Plasmônio de Superfície , Temperatura , Fatores de Virulência/genéticaRESUMO
Salmonella Gallinarum and Salmonella Enteritidis are genetically closely related however associated with different pathologies. Several studies have suggested that S. Gallinarum is less invasive in vitro than S. Enteritidis. In this study we confirm that the S. Gallinarum strains tested were much less invasive than the S. Enteritidis strains tested in cells of avian or human origin. In addition, the S. Gallinarum T3SS-1-dependent ability to invade host cells was delayed by two to three hours compared to S. Enteritidis, indicating that T3SS-1-dependent entry is less efficient in S. Gallinarum than S. Enteritidis. This was neither due to a decreased transcription of T3SS-1 related genes when bacteria come into contact with cells, as transcription of hilA, invF and sipA was similar to that observed for S. Enteritidis, nor to a lack of functionality of the S. Gallinarum T3SS-1 apparatus as this apparatus was able to secrete and translocate effector proteins into host cells. In contrast, genome comparison of four S. Gallinarum and two S. Enteritidis strains revealed that all S. Gallinarum genomes displayed the same point mutations in each of the main T3SS-1 effector genes sipA, sopE, sopE2, sopD and sopA.
Assuntos
Doenças das Aves Domésticas/microbiologia , Salmonelose Animal/microbiologia , Infecções por Salmonella/microbiologia , Salmonella enterica/fisiologia , Salmonella enterica/patogenicidade , Salmonella enteritidis/fisiologia , Salmonella enteritidis/patogenicidade , Animais , Aderência Bacteriana , Linhagem Celular , Linhagem Celular Tumoral , Galinhas , Humanos , Salmonella enterica/genética , Salmonella enteritidis/genéticaRESUMO
The infectious process of bacteria of the genus Salmonella requires the finely regulated use of various virulence factors. Among them, the type 3 secretion system-1 (T3SS-1) and the Rck and PagN invasins are involved in the internalization of the pathogen within eukaryotic cells, but their precise role in the host and in the pathogenic process is still poorly understood. In this study, we aimed to determine the kinetics of expression of these entry factors in a typhoid fever-like and a gastroenteritis model in mice by in vivo imaging using bioluminescent Salmonella Typhimurium reporter strains carrying chromosomal transcriptional fusions. Only pagN and T3SS-1 transcription has been clearly identified. Independently of the pathological model, the caecum was identified as the main transcription site of both pagN and the T3SS-1-encoding gene both at early and late stages of the infection. An intense transcription of pagN was also observed in deep organs in the typhoid fever-like model, while that of T3SS-1 remained quite sporadic in these organs, and mainly focused on the intestine all along the infection. This work will help to understand the respective role of these entry factors at the cellular level in the pathogenesis of Salmonella in vivo.
Assuntos
Febre Tifoide , Animais , Camundongos , Modelos Animais de Doenças , Salmonella typhimurium/genética , Intestinos , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismoRESUMO
Salmonella enterica subsp. enterica serotype Senftenberg is an emerging serotype in poultry production which has been found to persist in animals and the farm environment. We report the genome sequence and annotation of the SS209 strain of S. Senftenberg, isolated from a hatchery, which was identified as persistent in broiler chickens.
Assuntos
Genoma Bacteriano , Salmonella enterica/classificação , Salmonella enterica/genética , Cromossomos Bacterianos , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência MolecularRESUMO
Salmonella enterica subsp. enterica serotype Enteritidis is one of the major causes of gastroenteritis in humans due to consumption of poultry derivatives. Here we report the whole-genome sequence and annotation, including the virulence plasmid, of S. Enteritidis LA5, which is a chicken isolate used by numerous laboratories in virulence studies.
Assuntos
Genoma Bacteriano , Salmonella enterica/classificação , Salmonella enterica/genética , Cromossomos Bacterianos , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência MolecularRESUMO
BACKGROUND: Currently, food regulatory authorities consider all Listeria monocytogenes isolates as equally virulent. However, an increasing number of studies demonstrate extensive variations in virulence and pathogenicity of L. monocytogenes strains. Up to now, there is no comprehensive overview of the population genetic structure of L. monocytogenes taking into account virulence level. We have previously demonstrated that different low-virulence strains exhibit the same mutations in virulence genes suggesting that they could have common evolutionary pathways. New low-virulence strains were identified and assigned to phenotypic and genotypic Groups using cluster analysis. Pulsed-field gel electrophoresis, virulence gene sequencing and multi-locus sequence typing analyses were performed to study the genetic relatedness and the population structure between the studied low-virulence isolates and virulent strains. RESULTS: These methods showed that low-virulence strains are widely distributed in the two major lineages, but some are also clustered according to their genetic mutations. These analyses showed that low-virulence strains initially grouped according to their lineage, then to their serotypes and after which, they lost their virulence suggesting a relatively recent emergence. CONCLUSIONS: Loss of virulence in lineage II strains was related to point mutation in a few virulence genes (prfA, inlA, inlB, plcA). These strains thus form a tightly clustered, monophyletic group with limited diversity. In contrast, low-virulence strains of lineage I were more dispersed among the virulence strains and the origin of their loss of virulence has not been identified yet, even if some strains exhibited different mutations in prfA or inlA.
Assuntos
Variação Genética , Listeria monocytogenes/classificação , Listeria monocytogenes/patogenicidade , Fatores de Virulência/genética , Animais , Análise por Conglomerados , Modelos Animais de Doenças , Eletroforese em Gel de Campo Pulsado , Genótipo , Humanos , Listeria monocytogenes/isolamento & purificação , Camundongos , Dados de Sequência Molecular , Tipagem de Sequências Multilocus , Análise de Sequência de DNA , VirulênciaRESUMO
Applications of bioluminescence for the in vivo study of pathogenic microorganisms are numerous, ranging from the quantification of virulence gene expression to measuring the effect of antimicrobial molecules on the colonization of tissues and organs by the pathogen. Most studies are performed in mice, but recent works demonstrate that this technique is applicable to larger animals like fish, guinea pigs, ferrets, and chickens. Here, we describe the construction and the utilization of a constitutively luminescent strain of Salmonella Typhimurium to monitor in vivo and ex vivo the colonization of mice in the gastroenteritis, typhoid fever, and asymptomatic carriage models of Salmonella infection.
Assuntos
Galinhas , Infecções por Salmonella , Animais , Modelos Animais de Doenças , Furões , Cobaias , Camundongos , Salmonella typhimurium/genéticaRESUMO
Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide from Gram-negative bacteria, plays a major role in resistance of mice and humans to Salmonella infection. In chickens, Salmonella may establish a carrier state whereby bacteria are able to persist in the host organism without triggering clinical signs. Based on cellular morphological parameters, we developed a method, without using antibodies, to separate three cecal cell subpopulations: lymphocytes, enterocytes, and a population encompassing multiple cell types. We analyzed the mRNA expression of TLR4, interleukin-1ß (IL-1ß), IL-8, IL-12, and lipopolysaccharide-induced tumor necrosis factor alpha factor (LITAF) in cecal subpopulations of chicks from inbred lines resistant or susceptible to the carrier state infected with Salmonella enterica serovar Enteritidis. The results showed that resistance to the carrier state in chicks is associated with a larger percentage of lymphocytes and with higher levels of expression of TLR4 and IL-8 at homeostasis in the three cell subpopulations, as well as with a higher level of expression of LITAF in lymphocytes during the carrier state. In contrast to the early phase of infection, the carrier state is characterized by no major cell recruitment differences between infected and noninfected animals and no significant modification in terms of TLR4, IL-1ß, IL-8, IL-12, and LITAF expression in all cell subpopulations measured. However, TLR4 expression increased in the lymphocytes of chicks from the susceptible line, reaching the same level as that in infected chicks from the resistant line. These observations suggest that the carrier state is characterized by a lack of immune activation and highlight the interest of working at the level of the cell population rather than that of the organ.
Assuntos
Portador Sadio/imunologia , Expressão Gênica , Salmonelose Animal/imunologia , Salmonella enteritidis/imunologia , Receptor 4 Toll-Like/biossíntese , Animais , Portador Sadio/microbiologia , Ceco/imunologia , Galinhas , Citocinas/biossíntese , Enterócitos/imunologia , Perfilação da Expressão Gênica , Linfócitos/imunologiaRESUMO
The rck open reading frame (ORF) on the pefI-srgC operon encodes an outer membrane protein responsible for invasion of nonphagocytic cell lines and resistance to complement-mediated killing. Until now, the rck ORF was only detected on the virulence plasmids of three serovars of Salmonella subsp. enterica (i.e., Bovismorbificans, Enteritidis, and Typhimurium). The increasing number of Salmonella genome sequences allowed us to use a combination of reference sequences and whole-genome multilocus sequence typing (wgMLST) data analysis to probe the presence of the operon and of rck in a wide array of isolates belonging to all Salmonella species and subspecies. We established the presence of partial or complete operons in 61 subsp. enterica serovars as well as in 4 other subspecies with various syntenies and frequencies. The rck ORF itself was retrieved in 36 subsp. enterica serovars and in two subspecies with either chromosomal or plasmid-borne localization. It displays high conservation of its sequence within the genus, and we demonstrated that most of the allelic variations identified did not alter the virulence properties of the protein. However, we demonstrated the importance of the residue at position 38 (at the level of the first extracellular loop of the protein) in the invasin function of Rck. Altogether, our results highlight that rck is not restricted to the three formerly identified serovars and could therefore have a more important role in virulence than previously expected. Moreover, this work raises questions about the mechanisms involved in rck acquisition and about virulence plasmid distribution and evolution. IMPORTANCE The foodborne pathogen Salmonella is responsible for a wide variety of pathologies depending on the infected host, the infecting serovars, and its set of virulence factors. However, the implication of each of these virulence factors and their role in the specific host-pathogen interplay are not fully understood. The significance of our research is in determining the distribution of one of these factors, the virulence plasmid-encoded invasin and resistance to complement killing protein Rck. In addition to providing elements of reflection concerning the mechanisms of acquisition of specific virulence genes in certain serotypes, this work will help to understand the role of Rck in the pathogenesis of Salmonella.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Proteínas do Sistema Complemento/imunologia , Infecções por Salmonella/microbiologia , Salmonella enterica/genética , Salmonella typhimurium/genética , Sequência de Aminoácidos , Proteínas da Membrana Bacteriana Externa/química , Proteínas da Membrana Bacteriana Externa/imunologia , Humanos , Óperon , Plasmídeos/genética , Plasmídeos/imunologia , Infecções por Salmonella/imunologia , Salmonella enterica/imunologia , Salmonella enterica/patogenicidade , Salmonella typhimurium/imunologia , Salmonella typhimurium/patogenicidade , Alinhamento de Sequência , VirulênciaRESUMO
Salmonella Typhimurium expresses on its outer membrane the protein Rck which interacts with the epidermal growth factor receptor (EGFR) of the plasma membrane of the targeted host cells. This interaction activates signaling pathways, leading to the internalization of Salmonella. Since EGFR plays a key role in cell proliferation, we sought to determine the influence of Rck mediated infection on the host cell cycle. By analyzing the DNA content of uninfected and infected cells using flow cytometry, we showed that the Rck-mediated infection induced a delay in the S-phase (DNA replication phase) of the host cell cycle, independently of bacterial internalization. We also established that this Rck-dependent delay in cell cycle progression was accompanied by an increased level of host DNA double strand breaks and activation of the DNA damage response. Finally, we demonstrated that the S-phase environment facilitated Rck-mediated bacterial internalization. Consequently, our results suggest that Rck can be considered as a cyclomodulin with a genotoxic activity.
Assuntos
Proteínas da Membrana Bacteriana Externa , Salmonella typhimurium , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias , Divisão Celular , Membrana Celular , Salmonella typhimurium/genética , Transdução de SinaisRESUMO
Fimbriae play an important role in adhesion and are therefore essential for the interaction of bacteria with the environments they encounter. Most of them are expressed in vivo but not in vitro, thus making difficult the full characterization of these fimbriae. Here, we characterized the silencing of plasmid-encoded fimbriae (Pef) expression, encoded by the pef operon, in the worldwide pathogen Salmonella Typhimurium. We demonstrated that the nucleoid-associated proteins H-NS and Hha, and their respective paralogs StpA and YdgT, negatively regulate at pH 5.1 and pH 7.1 the transcription of the pef operon. Two promoters, PpefB and PpefA, direct the transcription of this operon. All the nucleoid-associated proteins silence the PpefB promoter and H-NS also targets the PpefA promoter. While Hha and YdgT are mainly considered as acting primarily through H-NS to modulate gene transcription, our results strongly suggest that Hha and YdgT silence pef transcription at acidic pH either by interacting with StpA or independently of H-NS and StpA. We also confirmed the previously described post-transcriptional repression of Pef fimbriae by CsrA titration via the fim mRNA and CsrB and CsrC sRNA. Finally, among all these regulators, H-NS clearly appeared as the major repressor of Pef expression. These results open new avenues of research to better characterize the regulation of these bacterial adhesive proteins and to clarify their role in the virulence of pathogens.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Ligação a DNA/genética , Proteínas de Fímbrias/genética , Fímbrias Bacterianas/genética , Proteínas Repressoras/genética , Salmonella typhimurium/genética , Regulação Bacteriana da Expressão Gênica , Chaperonas Moleculares/genética , Óperon , Regiões Promotoras Genéticas , Transcrição GênicaRESUMO
Salmonella is a facultative intracellular Gram-negative bacterium, responsible for a wide range of food- and water-borne diseases ranging from gastroenteritis to typhoid fever depending on hosts and serotypes. Salmonella thus represents a major threat to public health. A key step in Salmonella pathogenesis is the invasion of phagocytic and non-phagocytic host cells. To trigger its own internalization into non-phagocytic cells, Salmonella has developed different mechanisms, involving several invasion factors. For decades, it was accepted that Salmonella could only enter cells through a type three secretion system, called T3SS-1. Recent research has shown that this bacterium expresses outer membrane proteins, such as the Rck protein, which is able to induce Salmonella entry mechanism. Rck mimics natural host cell ligands and triggers engulfment of the bacterium by interacting with the epidermal growth factor receptor. Salmonella is thus able to use multiple entry pathways during the Salmonella infection process. However, it is unclear how and when Salmonella exploits the T3SS-1 and Rck entry mechanisms. As a series of reviews have focused on the T3SS-1, this review aims to describe the current knowledge and the limitations of our understanding of the Rck outer membrane protein. The regulatory cascade which controls Rck expression and the molecular mechanisms underlying Rck-mediated invasion into cells are summarized. The potential role of Rck-mediated invasion in Salmonella pathogenesis and the intracellular behavior of the bacteria following a Salmonella Rck-dependent entry are discussed.
Assuntos
Adesinas Bacterianas/metabolismo , Aderência Bacteriana , Endocitose , Infecções por Salmonella/microbiologia , Salmonella/fisiologia , Receptores ErbB/metabolismo , Regulação Bacteriana da Expressão Gênica , Ligação Proteica , Sistemas de Secreção Tipo III/metabolismoRESUMO
Brucella strains have been isolated since the 1990s from a wide variety of marine mammals and represent potential zoonotic pathogens. They have distinctive phenotypic and molecular characteristics from the terrestrial mammal Brucella species, and two new species names have been previously proposed based on DNA polymorphism at the omp2 locus and their preferential host, i.e. Brucella cetaceae for cetacean isolates and Brucella pinnipediae for pinniped isolates. The results presented in this study on characterization of these strains by infrequent restriction site-PCR (IRS-PCR), taking into account the higher number of IS711 elements in their genome compared to terrestrial mammal Brucella species, supports this classification. The nucleotide sequences of specific DNA fragments detected by IRS-PCR were determined and used to develop PCR identification tests for either B. cetaceae or B. pinnipediae.
Assuntos
Técnicas de Tipagem Bacteriana , Brucella/classificação , Mamíferos/microbiologia , Reação em Cadeia da Polimerase/métodos , Microbiologia da Água , Animais , Sequência de Bases , Brucella/genética , Brucella/isolamento & purificação , Bovinos , Primers do DNA/análise , Cães , Humanos , Oceanos e Mares , Filogenia , Polimorfismo de Fragmento de Restrição , Sensibilidade e EspecificidadeRESUMO
The immunogenicity and protective effect of a DNA vaccine encoding the heat-shock protein (Hsp) GroEL of Chlamydophila abortus AB7, an obligate intracellular bacterium that causes abortion in sheep, was evaluated in pregnant and non-pregnant mouse models. The C. abortus groEL gene was cloned by screening a genomic library constructed in lambdaFIX II arms with a nucleic acid probe corresponding to the central portion of the groEL gene from C. abortus. Sequence analysis of a positive clone revealed an open reading frame of 1632 bp encoding a 544 amino acid polypeptide with a predicted molecular mass of 58 256 Da and highly similar to GroEL of Chlamydia trachomatis (93 %) and Chlamydophila pneumoniae (94 %). As observed in other sequenced chlamydial genomes, the groEL gene belongs to an operon comprising another gene encoding the Hsp GroES. OF1 outbred mice were immunized intramuscularly with plasmid DNA carrying the groEL gene three times at 3 week intervals and challenged 2 weeks after the last DNA injection. In pregnant mice, no reduction in abortion was observed and the DNA vaccination failed to reduce the bacterial infection in the placenta and spleen of mice. Nevertheless, partial protection of fetuses was obtained. Immunization of non-pregnant mice with the groEL gene resulted in a specific humoral response with the predominant IgG2a isotype, suggesting a Th1-type immune response. The anti-GroEL antibodies showed no neutralizing effect in vitro on C. abortus infectivity. Although the DNA vaccine induced a delayed-type hypersensitivity response, it failed to elicit an efficient cellular immune response since the mice were not protected against bacterial challenge.
Assuntos
Aborto Espontâneo/prevenção & controle , Chaperonina 60/genética , Infecções por Chlamydophila/prevenção & controle , Clonagem Molecular , Complicações Infecciosas na Gravidez/prevenção & controle , Vacinas de DNA/imunologia , Animais , Animais não Endogâmicos , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/genética , Vacinas Bacterianas/imunologia , Chaperonina 60/imunologia , Chlamydophila/genética , Chlamydophila/imunologia , Infecções por Chlamydophila/microbiologia , Feminino , Camundongos , Dados de Sequência Molecular , Plasmídeos , Gravidez , Análise de Sequência de DNA , Vacinas de DNA/administração & dosagemRESUMO
The protective effect of DNA vaccination with the gene encoding the major outer-membrane protein (MOMP) of Chlamydophila abortus has been studied in non-pregnant and pregnant mouse models after chlamydial challenge. OF1 outbred mice were vaccinated intramuscularly three times every 3 weeks, mated and challenged with C. abortus 2 weeks after the last injection of DNA. In non-pregnant mice, the MOMP DNA vaccine elicited a specific humoral response with predominantly IgG2a antibodies, suggesting a Th1-type immune response. The induced antibodies showed no in vitro neutralizing effect on C. abortus infectivity. Moreover, immunization with the momp gene showed no reduction in the mean splenic bacterial counts of non-pregnant or pregnant mice or in the mean placental bacterial counts of pregnant mice after the C. abortus challenge. Nevertheless, the MOMP DNA immunization induced a non-specific and partial protection in fetuses against challenge.
Assuntos
Antígenos de Bactérias/imunologia , Proteínas da Membrana Bacteriana Externa/imunologia , Infecções por Chlamydophila/imunologia , Infecções por Chlamydophila/prevenção & controle , Chlamydophila/imunologia , Proteínas de Membrana/imunologia , Complicações Infecciosas na Gravidez/imunologia , Complicações Infecciosas na Gravidez/prevenção & controle , Vacinas de DNA/imunologia , Aborto Espontâneo/imunologia , Aborto Espontâneo/microbiologia , Aborto Espontâneo/prevenção & controle , Animais , Animais não Endogâmicos , Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/genética , Proteínas da Membrana Bacteriana Externa/genética , Feminino , Feto/imunologia , Feto/microbiologia , Imunoglobulina G/imunologia , Proteínas de Membrana/genética , Camundongos , Placenta/microbiologia , Gravidez , Complicações Infecciosas na Gravidez/microbiologia , Baço/microbiologia , Fatores de Tempo , Vacinas de DNA/genéticaRESUMO
Infection of chicken with Salmonella may lead to a carrier-state characterized by the persistence of bacteria in the ceca for a long period of time and result in their excretion in feces. This excretion is the source of contamination of their congeners and food. During infection, enterocytes are the primary target cells for Salmonella, the producers of soluble factors which launch immune response and cells which are reciprocally responsive to surrounding immune cells. This study used microarrays to compare the gene expression profile during carrier-state of enterocytes purified from infected and control chicks which are either resistant or susceptible to Salmonella Enteritidis carrier-state. In total, we identified 271 genes significantly differentially expressed with an absolute fold change greater than 1.5. A global analysis determined interaction networks between differentially regulated genes. Using an a priori approach, our analyses focused on differentially expressed genes which were transcriptionally linked to cytokines playing a major role in the fate of the immune response. The expression of genes transcriptionally linked to type I interferon and TGF-ß was down-regulated in infected chicks from both lines. Gene expression linked to the Th1 axis suggests the latter is inhibited in both lines. Finally, the expression of genes linked to IL-4, IL-5 and IL-13 indicates that susceptibility to carrier-state could be associated with a Th2 bias. Overall, these results highlight that the response to Salmonella during the acute phase and carrier-state is different and that enterocytes play a central role in this response.
Assuntos
Portador Sadio/veterinária , Galinhas , Regulação Bacteriana da Expressão Gênica/imunologia , Doenças das Aves Domésticas/microbiologia , Salmonelose Animal/imunologia , Salmonella enteritidis/imunologia , Animais , Portador Sadio/imunologia , Portador Sadio/microbiologia , Portador Sadio/transmissão , Suscetibilidade a Doenças , Enterócitos/imunologia , Enterócitos/microbiologia , Perfilação da Expressão Gênica/veterinária , Regulação Bacteriana da Expressão Gênica/genética , Interferon Tipo I/genética , Interferon Tipo I/imunologia , Análise de Sequência com Séries de Oligonucleotídeos/veterinária , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/transmissão , Reação em Cadeia da Polimerase em Tempo Real , Salmonelose Animal/microbiologia , Salmonelose Animal/transmissão , Salmonella enteritidis/genética , Organismos Livres de Patógenos Específicos , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/imunologiaRESUMO
Salmonella enterica, like many gram-negative pathogens, uses type three secretion systems (TTSS) to infect its hosts. The three TTSS of Salmonella, namely, TTSS-1, TTSS-2, and flagella, play a major role in the virulence of this bacterium, allowing it to cross the intestinal barrier and to disseminate systemically. Previous data from our laboratory have demonstrated the involvement of the chromosomal region harboring the yfgL, engA, and yfgJ open reading frames in S. enterica serovar Enteritidis virulence. Using microarray analysis and real-time reverse transcription-PCR after growth of bacterial cultures favorable for either TTSS-1 or TTSS-2 expression, we show in this study that the deletion in S. enterica serovar Enteritidis of yfgL, encoding an outer membrane lipoprotein, led to the transcriptional down-regulation of most Salmonella pathogenicity island 1 (SPI-1), SPI-2, and flagellar genes encoding the TTSS structural proteins and effector proteins secreted by these TTSS. In line with these results, the virulence of the DeltayfgL mutant was greatly attenuated in mice. Moreover, even if YfgL is involved in the assembly of outer membrane proteins, the regulation of TTSS expression observed was not due to an inability of the Delta yfgL mutant to assemble TTSS in its membrane. Indeed, when we forced the transcription of SPI-1 genes by constitutively expressing HilA, the secretion of the TTSS-1 effector protein SipA was restored in the culture supernatant of the mutant. These results highlight the crucial role of the outer membrane lipoprotein YfgL in the expression of all Salmonella TTSS and, thus, in the virulence of Salmonella. Therefore, this outer membrane protein seems to be a privileged target for fighting Salmonella.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Lipoproteínas/genética , Salmonella enteritidis/genética , Salmonella enteritidis/patogenicidade , Animais , Proteínas de Escherichia coli , Expressão Gênica , Perfilação da Expressão Gênica , Genes Bacterianos , Humanos , Camundongos , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcrição Gênica , VirulênciaRESUMO
DNA vaccination (also called genetic vaccination) recently celebrated its ten years of existence. This new method of immunization presents several advantages, including the induction of both humoral and cellular immune responses. This vaccination strategy has been very successful and has served as a basis for numerous experiments that had the aim of resolving parasitic, viral, and bacterial infections. In particular, DNA vaccination has been evaluated against Chlamydiaceae, small obligate intracellular bacteria, that induce many pathologies in humans and animals. Despite promising protective effects obtained in murine and turkey models with genes encoding outer membrane proteins and heat shock proteins, DNA vaccination against Chlamydiaceae must be optimized by further investigations and could benefit from the genomic sequencing in terms of the identification of new antigens.
Assuntos
Infecções por Chlamydiaceae/veterinária , Chlamydiaceae/imunologia , Vacinação/veterinária , Vacinas de DNA , Animais , Infecções por Chlamydiaceae/prevenção & controleRESUMO
Mice were intramuscularly immunized with a dnaK-encoding DNA plasmid. The protective effect of DNA immunization against Chlamydophila abortus infection was studied in pregnant and non-pregnant mice models. In non-pregnant mice, the dnaK vaccine induced a specific humoral response with the predominant IgG2a isotype, which failed to have in vitro neutralizing properties. No delayed-type hypersensitivity reaction was observed and the spleens of dnaK vaccinated-mice were not protected against C. abortus challenge. In pregnant mice, the dnaK vaccine induced a non-specific partial protection from abortion. This may be due to the immunogenic properties of the CpG motifs of bacterial DNA present in the vaccinal plasmid backbone. Nevertheless, spleens of dnaK vaccinated-pregnant mice were not protected.