RESUMO
The study investigated the influence of 4-methylbenzylidene camphor (4-MBC), daidzein, and estradiol-17ß-benzoate (E(2)) on either intact or osteotomized cancellous bone in ovariectomized (Ovx) rats. Three-month old Ovx rats were fed with soy-free (SF) diet over 8 weeks; thereafter, bilateral transverse metaphyseal osteotomy of tibia was performed and rats were divided into groups: rats fed with SF diet and SF diet supplemented with 4-MBC (200âmg), daidzein (50âmg), or E(2) (0.4âmg) per kilogram body weight. After 5 or 10 weeks, computed tomographical, biomechanical, histological, and ashing analyses were performed in lumbar spine and tibia of 12 rats from each group. 4-MBC and E(2) improved bone parameters in lumbar spine and tibia, were not favorable for osteotomy healing, and decreased serum osteocalcin level. However, daidzein improved bone parameters to a lesser extent and facilitated osteotomy healing. For lumbar spine, the bone mineral density was 338±9, 346±5, 361±6, and 360±5âmg/cm(3) in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. For tibia, the yield load was 98±5, 114±3, 90±2, and 52±4âN in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. Serum daidzein was 54±6âng/ml in daidzein group and equol was not detected. Alp and Igf1 genes were down-regulated in callus after daidzein and E(2) compared with 4-MBC (week 5). The response of bone tissue and serum markers of bone metabolism could be ordered: daidzein<4-MBCAssuntos
Osso e Ossos/efeitos dos fármacos
, Cânfora/análogos & derivados
, Estrogênios/farmacologia
, Isoflavonas/farmacologia
, Fosfatase Ácida/genética
, Fosfatase Alcalina/sangue
, Fosfatase Alcalina/genética
, Animais
, Fenômenos Biomecânicos
, Densidade Óssea/efeitos dos fármacos
, Doenças Ósseas Metabólicas/fisiopatologia
, Osso e Ossos/metabolismo
, Osso e Ossos/cirurgia
, Calo Ósseo/efeitos dos fármacos
, Calo Ósseo/metabolismo
, Cânfora/administração & dosagem
, Cânfora/farmacologia
, Dieta
, Estrogênios/administração & dosagem
, Feminino
, Expressão Gênica/efeitos dos fármacos
, Fator de Crescimento Insulin-Like I/genética
, Isoenzimas/genética
, Isoflavonas/administração & dosagem
, Vértebras Lombares/efeitos dos fármacos
, Vértebras Lombares/patologia
, Vértebras Lombares/fisiopatologia
, Osteocalcina/sangue
, Osteocalcina/genética
, Osteotomia
, Ratos
, Ratos Sprague-Dawley
, Reação em Cadeia da Polimerase Via Transcriptase Reversa
, Fosfatase Ácida Resistente a Tartarato
, Tíbia/efeitos dos fármacos
, Tíbia/patologia
, Tíbia/fisiopatologia
, Tomografia Computadorizada por Raios X/métodos