[Profiles of IgE sensitization to dust mite allergen components in patients with allergic rhinitis and asthma].

Objective:To study the differences and clinical significance of dust mite allergen components in allergic rhinitis（AR） and allergic rhinitis with asthma syndrome（ARAS） patients. Methods:The clinical data of 42 AR patients were retrospectively analyzed and patients were divided into AR and ARAS group. The serum sIgE concentrations of house dust mites were detected by ImmunoCAP system. The allergen components of Der p（Der p 1, Der p 2, Der p 7, Der p 10, Der p 21, Der p 23） and Der f（Der f 1, Der f 2） were analyzed by protein microarray method. The concentration differences of dust mite allergen and its components in AR and ARAS groups were analyzed. Results:Thirty-one cases of AR and 11 cases of ARAS were included. The positive rate of Der p and Der f was 100.0% and 97.6%, respectively. The highest sensitization rates of Der p allergen components were as following: Der p 1（73.8%）, Der f 1（66.7%）, Der f 2（64.3%） and Der p 2（61.9%）. The sensitization rates of Der f 1（100.0% vs 54.8%, P=0.006）, Der p 2（90.9% vs 51.6%, P=0.021） and Der f 2（100.0% vs 51.6%, P=0.004） in ARAS group were significantly higher than those in AR group. The sIgE concentrations of Der p in AR group were significantly lower than those in ARAS group（[7.65±12.15]kUA/L vs[15.20±18.77]kUA/L, P<0.05）. The sIgE concentrations of Der p 1（[5.39±4.61]kUA/L vs[2.03±2.97]kUA/L, P=0.013）, Der p 2（[8.82± 13.58]kUA/L vs[2.78±5.80]kUA/L, P=0.001）, Der p 23（[1.76± 3.88]kUA/L vs[0.28±0.65]kUA/L, P<0.001） was significantly higher in ARAS group than that of AR group. Correlation analysis showed that Der p 1, Der p 2, Der f 1 and Der f 2 had high positive correlation（P<0.01）. The dust mite components sensitization showed a multiple-sensitized mode. 66.7% of the 42 patients were positive for two or more components while it was 58.1% of the AR group and 90.9% of the ARAS group. The sensitization rate of 3 or more components in ARAS group was significantly higher than that in AR group（54.6% vs 29.1%, P<0.05）. Conclusion:The concentration of dust mites allergens in ARAS group is higher than that in AR group. Der p 1, Der f 1, Der p 2 and Der f 2 are the main allergen components with a higher sensitization rate in ARAS group. The concentrations of Der p 1, Der p 2 and Der p 23 were higher in ARAS group. The ARAS group is prone to multi-sensitzed to allergen components.

[Pollen allergen sensitization feature of seasonal allergic rhinitis in children and adolescents in northern China].

Objective:The prevalence of seasonal allergic rhinitis（SAR） is high in children and adolescents population. The aim of this study is to explore the feature of pollen allergen sensitization of SAR in children and adolescents . Method:Children and adolescents with self-reported seasonal allergic rhinitis in an allergy center of a tertiary hospital were enrolled and received skin prick test（SPT） of 13 common pollen allergens. SAR was defined with positive SPT to any pollen allergen. SPSS software was applied to analyze the differences in age, gender, sensitization pattern. Result:In total, 374（85.6%） SAR cases with self-reported seasonal allergic rhinitis were enrolled,including 213 males （57%） and 161 females （43%）. There are 198 children （2-12 years old） and 176 adolescents （13-17 years old）. The highest sensitization rate was presented in Chenopodium （73.8%） , Humulus（67.6%） and Artemisia （64.7%）.The severe sensitization was presented in Artemisia（53.7%）, Humulus（46.8%） and Chenopodium （42.0%）. No gender difference was found among different pollen allergens and sensitization patterns（P>0.05）. No age difference was found between different pollen allergens and sensitization patterns except that Cupressaceae was higher in adolescent group than that in children group（55.1% vs 42.9%, P=0.023）. Poly-sensitization rate was 57.2% in tree pollens, 59.6% in weed pollens and 81.3% in total. Significant correlation was found among different tree pollens and weed pollens（P<0.01）. Tree pollen sensitization was significantly correlated with weed pollens except Cupressaceae（P<0.01）. The mono-sensitization rate is low in all 4 weed pollen allergens ranged from 1% to 8% while 49.2% of SAR patients were allergic to all of the 4 weed pollen allergens. Conclusion:The prevalent pollen allergens in SAR were similar in children and adolescent comparing to adults. No obvious gender and age differences were found. An extensive co-sensitizations were found among pollens especially in weed pollens.

[Analysis of sensitization characteristics of artemisia pollen in the Inner Mongolian grassland region of China].

Objective:The distribution and sensitization characteristics of the pollen of artemisia were analyzed in the grasslands of Inner Mongolian. Method:A face-to-face questionnaires survey about the distribution and sensitization characteristics of the pollen of artemisia was performed together with pollen skin prick tests（SPTs） and measurements of the daily pollen count in 6 region（Xilinhot, Erenhot, Duolun, Tongliao, Jarud, Kailu） in the grasslands of Inner Mongolian. Result:A total of 6043 subjects completed the study. The prevalence of artemisia hay fever was 14.38%（869/6043）. The highest prevalence was found in the 18-39 age group（43.27%, 376/869）. Subjects from urban areas showed higher prevalence of AR than from rural areas（61.22% vs. 38.78%, P<0.01）. There were significant regional differences in the prevalence of AR（P<0.01） among the six areas investigated. The main clinical symptoms of artemisia hay fever were sneezing（96.78%） and itchy nose（91.60%）. The main clinical symptoms of ocular were itchy（68.58%）. Among associated symptoms of artemisia hay fever, fatigue and somnolence were common. The peak season of artemisia hay fever was in August. The season of onset of artemisia hay fever coincides with the peak time of pollen dispersal of artemisia. The SPT positive of artemisia pollen in the investigated 6043 people was 23.98%. Among comorbidities, allergic conjunctivitis accounts for 70.77% （615）, food allergy accounts for 85.85% （746）, asthma accounts for 9.55% （83）. Conclusion:The prevalence of artemisia hay fever in grassland of northern china stays at a high level. It is very important to control artemisia hay fever.