Enhanced Reactant Distribution in Redox Flow Cells.

Redox flow batteries (RFBs), provide a safe and cost-effective means of storing energy at grid-scale, and will play an important role in the decarbonization of global electricity networks. Several approaches have been explored to improve their efficiency and power density, and recently, cell geometry modification has shown promise in efforts to address mass transport limitations which affect electrochemical and overall system performance. Flow-by electrode configurations have demonstrated significant power density improvements in laboratory testing, however, flow-through designs with conductive felt remain the standard at commercial scale. Concentration gradients exist within these cells, limiting their performance. A new concept of redistributing reactants within the flow frame is introduced in this paper. This research shows a 60% improvement in minimum V3+ concentration within simulated vanadium redox flow battery (VRB/VRFB) cells through the application of static mixers. The enhanced reactant distribution showed a cell voltage improvement by reducing concentration overpotential, suggesting a pathway forward to increase limiting current density and cycle efficiencies in RFBs.

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities.

Untargeted metabolomics is a non-a priori analysis of biomolecules that characterizes the metabolome variations induced by short- and long-term exposures to stressors. Even if the metabolite annotation remains lacunar due to database gaps, the global metabolomic fingerprint allows for trend analyses of dose-response curves for hundreds of cellular metabolites. Analysis of dose/time-response curve trends (biphasic or monotonic) of untargeted metabolomic features would thus allow the use of all the chemical signals obtained in order to determine stress levels (defense or damage) in organisms. To develop this approach in a context of time-dependent microbial community changes, mature river biofilms were exposed for 1 month to four cobalt (Co) concentrations (from background concentration to 1 × 10-6 M) in an open system of artificial streams. The meta-metabolomic response of biofilms was compared against a multitude of biological parameters (including bioaccumulation, biomass, chlorophyll a content, composition and structure of prokaryotic and eukaryotic communities) monitored at set exposure times (from 1 h to 28 d). Cobalt exposure induced extremely rapid responses of the meta-metabolome, with time range inducing defense responses (TRIDeR) of around 10 s, and time range inducing damage responses (TRIDaR) of several hours. Even in biofilms whose structure had been altered by Co bioaccumulation (reduced biomass, chlorophyll a contents and changes in the composition and diversity of prokaryotic and eukaryotic communities), concentration range inducing defense responses (CRIDeR) with similar initiation thresholds (1.41 ± 0.77 × 10-10 M Co2+ added in the exposure medium) were set up at the meta-metabolome level at every time point. In contrast, the concentration range inducing damage responses (CRIDaR) initiation thresholds increased by 10 times in long-term Co exposed biofilms. The present study demonstrates that defense and damage responses of biofilm meta-metabolome exposed to Co are rapidly and sustainably impacted, even within tolerant and resistant microbial communities.

Cobalt effects on prokaryotic communities of river biofilms: Impact on their colonization kinetics, structure and functions.

Although cobalt (Co) plays a significant role in the transition to low-carbon technologies, its environmental impact remains largely unknown. This study examines Co impacts on the prokaryotic communities within river biofilms to evaluate their potential use as bioindicators of Co contamination. To this end, biofilms were cultivated in artificial streams enriched with different environmental Co concentrations (0.1, 0.5, and 1 µM Co) over 28 days and examined for prokaryotic abundance and diversity via quantitative PCR and DNA-metabarcoding every 7 days. The prokaryotic community's resilience was further investigated after an additional 35 days without Co contamination. The prokaryotic communities were affected by 0.5 and 1 µM Co from the onset of biofilm colonization. The biofilm biomass was comparable between treatments, but the community composition differed. Control biofilms were dominated by Cyanobacteria and Planctomycetes, whereas Bacteroidetes dominated the Co-contaminated biofilms. Potential functional redundancy was observed through the implementation of carbon fixation alternatives by non-photosynthetic prokaryotes in biofilms exposed to high Co concentrations. No structural resilience was observed in the biofilms after 35 days without Co contamination. Measuring the prokaryotic community structural response using molecular approaches appears to be a promising method for assessing shifts in water quality owing to Co contamination.

Comparative life cycle assessment and financial analysis of mixed culture polyhydroxyalkanoate production.

A life cycle assessment and financial analysis of mixed culture PHA (PHA(MC)) and biogas production was undertaken based on treating an industrial wastewater. Internal rate of return (IRR) and non-renewable CO(2)eq emissions were used to quantify financial viability and environmental impact. PHA(MC) was preferable to biogas production for treating the specified industrial effluent. PHA(MC) was also financially attractive in comparison to pure culture PHA production. Both PHA production processes had similar environmental impacts that were significantly lower than HDPE production. A large potential for optimisation exists for the PHA(MC) process as financial and environmental costs were primarily due to energy use for downstream processing. Under the conditions used in this work PHA(MC) was shown to be a viable biopolymer production process and an effective industrial wastewater treatment technology. This is the first study of its kind and provides valuable insight into the PHA(MC) process.

Rapid quantification of intracellular PHA using infrared spectroscopy: an application in mixed cultures.

Fourier transform infrared (FT-IR) spectroscopy is proposed for a method for rapid quantification of polyhydroxyalkanoates (PHA) in mixed culture bacterial systems. Spectra from 122 samples from a wide range of PHA production systems were studied. The spectra were collected in a library that was used to calibrate a partial least squares (PLS) model linking FT-IR spectra with PHA content in the biomass. The library of spectra contained samples with a range of total PHA content (0.03-0.58 w/w) as well as varying compositions (poly-(3-hydroxyvalerate) (3HV) content of 0-63% in Cmol basis). A robust PLS model was developed using calibration data from a diverse range of systems and PHA content. Coupling this model with FT-IR spectra has been shown to be applicable for predicting PHA content in mixed culture production systems. The method was used to reliably determine PHA content in biomass from a new, independent PHA production system with a standard error of prediction (RMSEP) value of 0.023 w/w, despite the complexity of the matrices. This method reduces the analytical time for PHA quantification down to under 30 min (5 min handling time was achieved when FT-IR equipment was immediately available), and eliminates hazardous waste by-products. The work has demonstrated a level of accuracy and reproducibility in quantifying PHA in mixed culture systems similar to that obtained from the GC analytical technique. Further work is required to enable the use of the method to analyze crystallinity related factors that may be useful towards quantifying poly-(3-hydroxybutyrate) and poly-(3-hydroxyvalerate) (3HB/3HV) composition. The method has been shown to be suitable for rapid quantification in large scale applications and in its present form is reliable for routine process monitoring.