Multiple wheat genomes reveal global variation in modern breeding.

Advances in genomics have expedited the improvement of several agriculturally important crops but similar efforts in wheat (Triticum spp.) have been more challenging. This is largely owing to the size and complexity of the wheat genome1, and the lack of genome-assembly data for multiple wheat lines2,3. Here we generated ten chromosome pseudomolecule and five scaffold assemblies of hexaploid wheat to explore the genomic diversity among wheat lines from global breeding programs. Comparative analysis revealed extensive structural rearrangements, introgressions from wild relatives and differences in gene content resulting from complex breeding histories aimed at improving adaptation to diverse environments, grain yield and quality, and resistance to stresses4,5. We provide examples outlining the utility of these genomes, including a detailed multi-genome-derived nucleotide-binding leucine-rich repeat protein repertoire involved in disease resistance and the characterization of Sm16, a gene associated with insect resistance. These genome assemblies will provide a basis for functional gene discovery and breeding to deliver the next generation of modern wheat cultivars.

Lateral spatial biases in naturalistic and simulated driving: Does pseudoneglect influence performance?

Whereas a rightward bump is more likely than a leftward bump when walking through a doorway, investigations into potential similar asymmetries for drivers are limited. The research presented here aims to determine the influence of innate lateral spatial biases when driving. Data from the Strategic Highway Research Program Naturalistic Driving Study (SHRP 2 NDS) and a driving simulation were used to address our research questions. Data points from SHRP 2 were aggregated within relevant variables (e.g., left/right obstacles). In the simulation, participants drove in ways that were consistent with their everyday driving in urban and rural environments. Collision frequency, collision severity and average lateral lane position were analyzed with rightward biases throughout both analyzes. SHRP 2 data indicated greater likelihoods of collisions when vehicles crossed the right line/edge of the road and when making a right turn. There were more collisions with obstacles on the right side, which were also more severe, and greater rightward lane deviations in the driving simulation, contrasted with more severe collisions on the left side in SHRP 2 data, possibly because of the presence of traffic. These findings suggest that previously observed rightward biases in distant space when walking are also present when driving.

Lateral biases in aesthetic and spatial location judgments: differences between tasks and native reading directions.

People exhibit consistent leftward spatial biases across a variety of tasks. However, individuals with a native reading direction other than left-to-right (LTR) show an attenuation of the leftward bias. The current study used procedurally similar tasks to examine spatial ability and aesthetic preferences in LTR and right-to-left (RTL) groups. In the spatial task participants viewed a centred rectangle partially occluded by an overlapping circle and estimated the centre of the circle with a single mouse click. In the aesthetic task participants used the mouse to control a "virtual flashlight" to light images of abstract paintings in the most aesthetically pleasing way. Contrary to predictions, smaller errors were made for circles on the right and estimations were progressively less accurate as circle size increased in the spatial task. On the aesthetic task, light placements of LTR participants were biased to the left and significantly different from the slightly rightward placements of RTL participants. As predicted, when completing the aesthetics task amounts of time scanning left or right visual space were different between groups. Findings support the theory that directional scanning biases attenuate leftward lateral biases and further, the nature of the visuospatial task may vary the strength of lateral bias.

Examining preferences for website support to parents of adolescents with diabetes.

Diabetes can be stressful as parents seek optimal outcomes for their adolescent with type 1 diabetes. This study examined parents' interest and perspectives related to online diabetes resources. Based on a qualitative description approach, 14 qualitative group interviews were conducted with (i) parents of adolescents with diabetes (n = 29), and (ii) pediatric health care providers (n = 31). Participants were recruited, through a purposive sampling approach, at pediatric centers in three Canadian cities. Qualitative data were subjected to thematic analysis comprising data coding, categorization, and ultimate theme generation. Participants described parental care for adolescents with diabetes as complex and reflective of difficult and nuanced tasks. They recommended the development of a comprehensive parent-based information and support website, and identified crucial elements of the website. Overarching themes comprised the following: complex parenting processes in diabetes care, parents' need for information and support, challenges and benefits of online support, key elements of an online resource, and caution regarding online resources. Based on these findings, website information and support emerged as a viable and desired resource for augmenting pediatric care within clinical settings. Caution was also offered in addressing potential challenges inherent in online support. Findings offer guidance for online support to parents.

Dorsal stream contributions to perceptual asymmetries.

Neurologically normal individuals show a bias toward the left side of space, referred to as pseudoneglect due to its similarity to clinical hemispatial neglect. The left bias appears to be stronger in the lower visual field during free-viewing, which could result from preferential dorsal stream processing. The current experiments used modified greyscales tasks, incorporating motion and isoluminant color, to explore whether targeting dorsal or ventral stream processing influenced the strength of the left bias. It was expected that the left bias would be stronger on the motion task than on a task incorporating isoluminant color. In Study 1, similar left biases were observed during prolonged viewing for luminance, motion and red, but not green color. The unexpected finding of a leftward bias for red under prolonged viewing was replicated in Study 2. A leftward bias for motion was also evident during 150 ms viewing in Study 2. In Study 3, the left bias was not apparent when using a blue/yellow condition, suggesting the left bias for red under prolonged viewing was likely unique to red. Furthermore, the leftward bias for red disappeared under brief viewing conditions. It is suggested that dorsal stream processing likely underlies visual field differences in pseudoneglect.

Which emphasis technique to use? Perception of emphasis techniques with varying distractors, backgrounds, and visualization types.

Emphasis effects are visual changes that make data elements distinct from their surroundings. Designers may use computational saliency models to predict how a viewer's attention will be guided by a specific effect; however, although saliency models provide a foundational understanding of emphasis perception, they only cover specific visual effects in abstract conditions. To address these limitations, we carried out crowdsourced studies that evaluate emphasis perception in a wider range of conditions than previously studied. We varied effect magnitude, distractor number and type, background, and visualization type, and measured the perceived emphasis of 12 visual effects. Our results show that there are perceptual commonalities of emphasis across a wide range of environments, but also that there are limitations on perceptibility for some effects, dependent on a visualization's background or type. We developed a model of emphasis predictability based on simple scatterplots that can be extended to other viewing conditions. Our studies provide designers with new understanding of how viewers experience emphasis in realistic visualization settings.

Visualization Tools for Genomic Conservation.

SynVisio and Accusyn ( genomevis.usask.ca ) are freely available web-based tools for visualizing genomic conservation that provide easy-to-access visualizations for researchers to interact with their datasets and change parameters in real time to carry out synteny exploration and analysis through multiple coordinated visual representations. The tools use standard file formats and outputs from existing synteny detection systems such as MCScanX or DAGChainer, and provide several features that are valuable for large-scale genomic analysis: a range of visualization scales from full genomes down to single collinearity blocks; single-level and multiple-level plots that enable the analysis of more than two genomic regions; annotation tracks that can be loaded using standard BedGraph files; several techniques for reducing visual clutter in visualizations; the ability to download high-quality images of the visualizations; and a snapshot panel for storing configurations of the interface for later revisitation.

Exploiting High-Throughput Indoor Phenotyping to Characterize the Founders of a Structured B. napus Breeding Population.

Phenotyping is considered a significant bottleneck impeding fast and efficient crop improvement. Similar to many crops, Brassica napus, an internationally important oilseed crop, suffers from low genetic diversity, and will require exploitation of diverse genetic resources to develop locally adapted, high yielding and stress resistant cultivars. A pilot study was completed to assess the feasibility of using indoor high-throughput phenotyping (HTP), semi-automated image processing, and machine learning to capture the phenotypic diversity of agronomically important traits in a diverse B. napus breeding population, SKBnNAM, introduced here for the first time. The experiment comprised 50 spring-type B. napus lines, grown and phenotyped in six replicates under two treatment conditions (control and drought) over 38 days in a LemnaTec Scanalyzer 3D facility. Growth traits including plant height, width, projected leaf area, and estimated biovolume were extracted and derived through processing of RGB and NIR images. Anthesis was automatically and accurately scored (97% accuracy) and the number of flowers per plant and day was approximated alongside relevant canopy traits (width, angle). Further, supervised machine learning was used to predict the total number of raceme branches from flower attributes with 91% accuracy (linear regression and Huber regression algorithms) and to identify mild drought stress, a complex trait which typically has to be empirically scored (0.85 area under the receiver operating characteristic curve, random forest classifier algorithm). The study demonstrates the potential of HTP, image processing and computer vision for effective characterization of agronomic trait diversity in B. napus, although limitations of the platform did create significant variation that limited the utility of the data. However, the results underscore the value of machine learning for phenotyping studies, particularly for complex traits such as drought stress resistance.

Directional collisions during a route-following task.

Neurologically normal people tend to collide with objects on the right side more frequently than with objects located on the left side of space. This phenomenon could be attributable to pseudoneglect wherein individuals selectively attend to the left field. The current study investigated this effect using a virtual route-following task that was presented centrally, in the lower field, and in the upper field. Handedness was also examined. Fifty-two participants (four left handed) completed this task, and when presented in the lower field, more left-side collisions emerged. In the upper condition, this bias reversed direction to the expected rightward bias. In the central condition, there was no significant directional bias in collision behavior. An interaction between handedness and presentation condition indicated that left-handed participants experienced more right-side collisions in the central condition. Collectively, these results suggest that directional biases (i.e., left vs. right) in collision behavior are modulated by both location in the visual field (central, upper, or lower) and handedness.

Men are more accurate than women in aiming at targets in both near space and extrapersonal space.

Men excel at motor tasks requiring aiming accuracy whereas women excel at different tasks requiring fine motor skill. However, these tasks are confounded with proximity to the body, as fine motor tasks are performed proximally and aiming tasks are directed at distal targets. As such, it is not known whether the male advantage on tasks requiring aiming accuracy is because men have better aim or is better in the proximal domain in which the task is usually presented. 18 men (M age = 20.6 yr., SD = 3.0) and 20 women (M age = 18.7 yr., SD = 0.9) performed 2 tasks of extrapersonal aiming accuracy (>2 m away), 2 tasks of aiming accuracy performed in near space (< 1 m from them), and a task of fine motor skill. Men outperformed women on both the extrapersonal aiming tasks, and women outperformed men on the task of fine motor skill. However, a male advantage was observed for one of the aiming tasks performed in near space, suggesting that the male advantage for aiming accuracy does not result from proximity.

A collaborative document repository for home care teams.

Home care workers are mobile, work out of different locations, and have a high level of uncertainty in their schedules. This makes communication and information sharing difficult, and workers are often unable to account for others' activities when planning their own treatments. To address this issue, we developed and evaluated a clinical information system for home care that supports current paperwork practices and stores documents in a central repository that is accessible by all workers that treat a patient.

Supporting collaboration in multidisciplinary home care teams.

Collaboration is an important part of healthcare delivery. However, in home care, collaboration is difficult due to the mobility and schedule variability of the workers. In this paper, we investigate the difficulties inherent in home care collaboration. We present the results of a study carried out with home care clinicians in Saskatoon District Health, and identify five areas of collaboration that are difficult for home care workers: scheduling, information dissemination, information retrieval, short-term treatment coordination, and long-term treatment planning. We present recommendations for incorporating support for each of these areas into point-of-care clinical information systems that provide access to shared patient records. Finally, we discuss general design approaches for incorporating this type of support, including the need for workers to maintain awareness of the activities of others, and the need to integrate communication with the presentation of the health record.

Awareness-based scheduling in a home care clinical information system.

In home care, workers from different disciplines treat patients in their homes. However, workers who treat a shared patient are mobile and rarely see each other face-to-face, so it is difficult for them to maintain awareness of others' activities. In this paper, we discuss one aspect of these information limitations by considering how limited awareness of others' schedules influences work patterns in home care. We identify and discuss several problems that arise from incomplete information about others' schedules. We examine design approaches for supporting mutual awareness of others' schedules by presenting Mohoc, a point-of-care clinical information system for workers in Saskatoon District Health, and the results of a 2(1/2)-month field trial we carried out with the system.

Female advantage for spatial location memory in both static and dynamic environments.

A female advantage has previously been found for spatial location tests of object memory. Previous studies have used static, 2-D tasks to test this advantage. This study used a computerized adaptation of the game Concentration to test object location memory in both a static and dynamic array of 24 pairs of line drawings. The dynamic version of this task was used to better reflect the dynamic real world in which we usually use object location memory. Consistent with previous research, we observed a female advantage. This advantage was found to a similar extent in both the static and dynamic versions of the task. The female advantage for object location memory is a concrete advantage in spatial cognition that females show on the Concentration Task, regardless of the nature of the presentation environment.