Predicting and optimizing reactive oxygen species metabolism in Punica granatum L. through machine learning: role of exogenous GABA on antioxidant enzyme activity under drought and salinity stress.

BACKGROUND: Drought and salinity stress have been proposed as the main environmental factors threatening food security, as they adversely affect crops' agricultural productivity. As a potential solution, the application of plant growth regulators to enhance drought and salinity tolerance has gained considerable attention. γ-aminobutyric acid (GABA) is a four-carbon non-protein amino acid that accumulates in plants as a response to stressful conditions. This study focused on a comparative assessment of several machine learning (ML) regression models, including radial basis function, generalized regression neural network (GRNN), random forest (RF), and support vector regression (SVR) to develop predictive models for assessing the effect of different concentrations of GABA (0, 10, 20, and 40 mM) on various physio-biochemical traits during periods of drought, salinity, and combined stress conditions. The physio-biochemical traits included antioxidant enzyme activities (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; and ascorbate peroxidase, APX), protein content, malondialdehyde (MDA) levels, and hydrogen peroxide (H2O2) levels. The non­dominated sorting genetic algorithm­II (NSGA­II) was employed for optimizing the superior prediction model. RESULTS: The GRNN model outperformed the other ML algorithms and was therefore selected for optimization by NSGA-II. The GRNN-NSGA-II model revealed that treatment with GABA at concentrations of 20.90 mM and 20.54 mM, under combined drought and salinity stress conditions at 20.86 and 20.72 days post-treatment, respectively, could result in the maximum values for protein content (by 0.80 and 0.69), APX activity (by 50.63 and 51.51), SOD activity (by 0.54 and 0.53), POD activity (by 1.53 and 1.72), CAT activity (by 4.42 and 5.66), as well as lower MDA levels (by 0.12 and 0.15) and H2O2 levels (by 0.44 and 0.55), respectively, in the 'Atabaki' and 'Rabab' cultivars. CONCLUSIONS: This study demonstrates that the GRNN-NSGA-II model, as an advanced ML algorithm with a strong predictive ability for outcomes in combined stressful environmental conditions, provides valuable insights into the significant factors influencing such multifactorial processes.

Variations in photoperiods and their impact on yield, photosynthesis and secondary metabolite production in basil microgreens.

BACKGROUND: The effects of different photoperiods on plant phytochemical synthesis can be improved by adjusting the daily light integral. Photoperiod is one of the most important environmental factors that control growth, plant's internal rhythm and the synthesis of secondary metabolites. Information about the appropriate standard in terms of photoperiod for growing basil microgreens as one of the most important medicinal plants is limited. In this study, the effects of five different photoperiods, 6 (6 h × 3 cycles), 8 (8 h × 2 cycles), 16, 18, and 24 h day- 1 on the yield, photosynthesis and synthesis of secondary metabolites of three cultivars and one genotype of basil microgreens in floating system were evaluated. The purpose of this research was to determine the feasibility of using permanent light in growing basil microgreens and to create the best balance between beneficial secondary metabolites and performance. RESULTS: The results showed that the effects of photoperiod and cultivar on all investigated traits and their interaction on photosynthetic pigments, antioxidant capacity, total phenolic compounds, proline content and net photosynthesis rate were significantly different at the 1% level. The highest levels of vitamin C, flavonoids, anthocyanins, yield and antioxidant potential composite index (APCI) were obtained under the 24-h photoperiod. The highest antioxidant capacity was obtained for the Kapoor cultivar, and the highest total phenolic compound and proline contents were measured for the Ablagh genotype under a 24-h photoperiod. The highest yield (4.36 kg m- 2) and APCI (70.44) were obtained for the Ablagh genotype. The highest nitrate content was obtained with a photoperiod of 18 h for the Kapoor cultivar. The highest net photosynthesis rate was related to the Violeto cultivar under a 24-hour photoperiod (7.89 µmol CO2 m- 2 s- 1). Antioxidant capacity and flavonoids had a positive correlation with phenolic compounds and vitamin C. Yield had a positive correlation with antioxidant capacity, flavonoids, vitamin C, APCI, and proline. CONCLUSIONS: Under continuous light conditions, basil microgreens resistance to light stress by increasing the synthesis of secondary metabolites and the increase of these biochemical compounds made basil microgreens increase their performance along with the increase of these health-promoting compounds. The best balance between antioxidant compounds and performance was achieved in continuous red + blue light. Based on these results, the use of continuous artificial LED lighting, due to the increase in plant biochemical with antioxidant properties and yield, can be a suitable strategy for growing basil microgreens in floating systems.

Selenium and its nanoparticles modulate the metabolism of reactive oxygen species and morpho-physiology of wheat (Triticum aestivum L.) to combat oxidative stress under water deficit conditions.

BACKGROUND: Wheat (Triticum aestivum L.) is one of the most important cereal crop species worldwide, but its growth and development are adversely influenced by drought stress. However, the application of trace elements is known to improve plant physiology under water-limited conditions. In this study, the effects of drought stress on wheat plants were investigated, with a focus on potential mitigation by foliar application of selenium nanoparticles (Se(np)) and sodium selenate (Na2SeO4). The experiment was conducted in a net house using a completely randomized design with four replications. The treatments involved three levels of drought stress (mild, moderate, and severe) started at 30 days after sowing (DAS), with foliar sprays of Se(np) and Se (both 25 µM) initiated at 27 DAS and repeated 4 times at 7-day intervals until 55 DAS. RESULTS: Drought stress significantly reduced plant growth, whereas Se(np) and Se sprays enhanced it. Drought stress induced chlorophyll degradation, increased malondialdehyde and hydrogen peroxide levels, impaired membrane stability, and caused electrolyte leakage. Severe drought stress reduced the levels of antioxidants (e.g., proline, ascorbate, and glutathione by 4.18-fold, 80%, and 45%) and the activities of antioxidant enzymes (ascorbate peroxidase, dehydroascorbate reductase, and others). Conversely, treatment with Se(np) and Se restored these parameters, for example, 1.23-fold higher total chlorophyll content with Se(np) treatment, 26% higher APX activity with Se treatment, 15% lower electrolyte leakage with Se treatment in wheat plants under severe drought stress. This Se-associated enhancement facilitated rapid scavenging of reactive oxygen species and reduced methylglyoxal toxicity, thereby diminishing oxidative stress and positively affecting the morphophysiological and biochemical responses of the plants under drought. CONCLUSIONS: Drought-stressed wheat plants exhibited reductions in physiological processes, including water uptake and photosynthetic activity. However, Se(np) and Se applied at 25 µM mitigated the detrimental effects of drought. The application of Se(np) was notably more effective than the application of Se in mitigating drought stress, indicating the potential of the application of Se(np) as a sustainable agricultural practice under water-limited conditions.

2,4-D mediated moderation of aluminum tolerance in Salvinia molesta D. Mitch. with regards to bioexclusion and related physiological and metabolic changes.

We examined the efficacy of 2,4-dichlorophenoxy acetic acid (2,4-D; 500 µM) in enhancing the potential of Salvinia species for tolerance to aluminum (Al) toxicity (240 and 480 µM, seven days). Salvinia showed better efficacy in removal of toxicity of Al by sorption mechanism with changes of bond energy shifting on cell wall residues and surface structure. Plants recorded tolerance to Al concentration (480 µM) when pretreated with 2,4-D through adjustment of relative water content, proline content, osmotic potential, and improved the pigment fluorescence for energy utilization under Al stress. Photosynthetic activities with regards to NADP-malic enzyme and malic dehydrogenase and sugar metabolism with wall and cytosolic invertase activities were strongly correlated with compatible solutes. A less membrane peroxidation and protein carbonylation had reduced ionic loss over the membrane that was studied with reduced electrolyte leakage with 2,4-D pretreated plants. Membrane stabilization was also recorded with higher ratio of K+ to Na+, thereby suggesting roles of 2,4-D in ionic balance. Better sustenance of enzymatic antioxidation with peroxidase and glutathione metabolism reduced reactive oxygen species accumulation and save the plant for oxidative damages. Moreover, gene polymorphism for antioxidant, induced by 2,4-D varied through Al concentrations would suggest an improved biomarker for tolerance. Collectively, analysis and discussion of plant's responses assumed that auxin herbicide could be a potential phytoprotectant for Salvinia as well as improving the stability to Al toxicity and its bioremediation efficacy.

In previous reports, aquatic weeds, particularly, from pteridophytic flora have been exercised, however, in less frequent. Aluminum (Al) toxicity, being a major problem, specifically with respect to cultivated crops like rice and vegetables, is a serious issue in alkaline soil. In context to growth of Salvinia in the areas of low lands where few important crops like rice are frequently cultivated. Therefore, Al toxicity with regards to rice cultivation in low land conditions, which is habitat for Salvinia, could be interesting. Thus, decontamination of low land for salinity with aquatic environment can be remediated with biological materials where Salvinia would be a choice. This would be something new in studies for the aquatic weeds over the existing database. Moreover, 2,4-dichlorophenoxy acetic acid (2,4-D) being a common herbicide in agricultural field that becomes more problematic with metal toxicity is another focus for physiological responses with Salvinia. The adoption and sustainability of Salvinia against 2,4-D may highlight insights for physiological activities would be the biomarker for herbicide toxicity.

Carbon dioxide sensitization delays the postharvest ripening and fatty acids composition of Capsicum fruit by regulating ethylene biosynthesis, malic acid and reactive oxygen species metabolism.

Present study would be significant in the sustenance of quality characters for postharvest storage of Capsicum fruit with CO2-sensitization in biocompatible manner. The present experiment describes effects of CO2 sensitization on delaying postharvest ripening through physiological attributes in Capsicum fruit. The experiment was conducted with acidified bicarbonate-derived CO2 exposure for 2 h on Capsicum fruit, kept under white light at 25 °C through 7 days postharvest storage. Initially, fruits responded well to CO2 as recorded sustenance of greenness and integrity of fruit coat resolved through scanning electron micrograph. Loss of water and accumulation of total soluble solids were marginally increased on CO2-sensitized fruit as compared to non-sensitized (control) fruit. The ethylene metabolism biosynthetic genes like CaACC synthase, CaACC oxidase were downregulated on CO2-sensitization. Accompanying ethylene metabolism cellular respiration was downregulated on CO2 induction as compared to control through 7 days of storage. Fruit coat photosynthesis decarboxylating reaction by NADP malic enzyme was upregulated to maintain the reduced carbon accumulation as recorded on 7 days of storage under the same condition. CO2-sensitization effectively reduced the lipid peroxides as oxidative stress products on ripening throughout the storage. Anti-oxidation reaction essentially downregulates the ROS-induced damages of biomolecules that otherwise are highly required for food preservation during postharvest storage. Thus, the major finding is that CO2-sensitization maintains a higher ratio of unsaturated to saturated fatty acids in fruit coat during storage. Tissue-specific downregulation of ROS also maintained the nuclear stability under CO2 exposure. These findings provide basic as well as applied insights for sustaining Capsicum fruit quality with CO2 exposure under postharvest storage. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-024-01471-4.

Supplementation with Ascophyllum nodosum extracts mitigates arsenic toxicity by modulating reactive oxygen species metabolism and reducing oxidative stress in rice.

Ascophyllum nodosum extract (ANE) is considered as an effective source of biostimulants that have the potential of ameliorating the negative impact of different abiotic stresses in plants. Considering the growth-promoting ability and other regulatory roles of ANE, the present investigation was executed to evaluate the role of ANE in conferring arsenic (As) tolerance in rice (Oryza sativa L. cv. BRRI dhan89). Rice seedlings (35-d-old) were exposed to two doses of sodium arsenate (As1 - 50 mg As kg-1 soil; As2 - 100 mg As kg-1 soil) at 25 days after transplanting through irrigation, whereas only water was applied to the control. Foliar application of 0.1% ANE was also supplemented under control as well as As-stressed conditions at 7 days intervals for 5 times. Arsenic-induced oxidative stress was evident through a sharp increase in lipid peroxidation, hydrogen peroxide, methylglyoxal, and electrolyte leakage in the As-treated plants. As a consequence, plant growth and biomass, leaf relative water content, as well as yield attributes were reduced noticeably. On the other hand, ANE supplemented plants accumulated enhanced levels of ascorbate and glutathione, their redox balance, and the activities of antioxidant and glyoxalase enzymes viz. ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, glutathione reductase, catalase, glutathione peroxidase, and activities of glyoxalase I and glyoxalase II, respectively. Furthermore, relative water content, plant growth, yield attributes and yield were increased in the As-treated rice plants with ANE supplementation. The results reflected that foliar spray with ANE alleviated As-induced oxidative stress in rice plants by modulating the antioxidative defense and glyoxalase system.

Plant Responses and Tolerance to Salt Stress: Physiological and Molecular Interventions 2.0.

Environmental problems are pervasive and significantly impact a variety of plant species, which are affected by two broad types of conditions: abiotic and biotic stress [...].

Crop microbiome: their role and advances in molecular and omic techniques for the sustenance of agriculture.

MAIN CONCLUSION: This review is an effort to provide in-depth knowledge of microbe's interaction and its role in crop microbiome using combination of advanced molecular and OMICS technology to translate this information for the sustenance of agriculture. Increasing population, climate change and exhaustive agricultural practices either influenced nutrient inputs of soil or generating biological and physico-chemical deterioration of the soils and affecting the agricultural productivity and agro-ecosystems. Alarming concerns toward food security and crop production claim for renewed attention in microbe-based farming practices. Microbes are omnipresent (soil, water, and air) and their close association with plants would help to accomplish sustainable agriculture goals. In the last few decades, the search for beneficial microbes in crop production, soil fertilization, disease management, and plant growth promotion is the thirst for eco-friendly agriculture. The crop microbiome opens new paths to utilize beneficial microbes and manage pathogenic microbes through integrated advanced biotechnology. The crop microbiome helps plants acquire nutrients, growth, resilience against phytopathogens, and tolerance to abiotic stresses, such as heat, drought, and salinity. Despite the emergent functionality of the crop microbiome as a complicated constituent of the plant fitness, our understanding of how the functionality of microbiome influenced by numerous factors including genotype of host, climatic conditions, mobilization of minerals, soil composition, nutrient availability, interaction between nexus of microbes, and interactions with other external microbiomes is partially understood. However, the structure, composition, dynamics, and functional contribution of such cultured and uncultured crop microbiome are least explored. The advanced biotechnological approaches are efficient tools for acquiring the information required to investigate the microbiome and extract data to develop high yield producing and resistant variety crops. This knowledge fills the fundamental gap between the theoretical concepts and the operational use of these advanced tools in crop microbiome studies. Here, we review (1) structure and composition of crop microbiome, (2) microbiome-mediated role associated with crops fitness, (3) Molecular and -omics techniques for exploration of crop microbiome, and (4) current approaches and future prospectives of crop microbiome and its exploitation for sustainable agriculture. Recent -omic approaches are influential tool for mapping, monitoring, modeling, and management of crops microbiome. Identification of crop microbiome, using system biology and rhizho-engineering, can help to develop future bioformulations for disease management, reclamation of stressed agro-ecosystems, and improved productivity of crops. Nano-system approaches combined with triggering molecules of crop microbiome can help in designing of nano-biofertilizers and nano-biopesticides. This combination has numerous merits over the traditional bioinoculants. They stimulate various defense mechanisms in plants facing stress conditions; provide bioavailability of nutrients in the soil, helps mitigate stress conditions; and enhance chances of crops establishment.

Plant Responses and Tolerance to Salt Stress: Physiological and Molecular Interventions.

Salinity is considered one of the most devastating environmental stresses that drastically curtails the productivity and quality of crops across the world [...].

Nitrate-Nitrite-Nitric Oxide Pathway: A Mechanism of Hypoxia and Anoxia Tolerance in Plants.

Oxygen (O2) is the most crucial substrate for numerous biochemical processes in plants. Its deprivation is a critical factor that affects plant growth and may lead to death if it lasts for a long time. However, various biotic and abiotic factors cause O2 deprivation, leading to hypoxia and anoxia in plant tissues. To survive under hypoxia and/or anoxia, plants deploy various mechanisms such as fermentation paths, reactive oxygen species (ROS), reactive nitrogen species (RNS), antioxidant enzymes, aerenchyma, and adventitious root formation, while nitrate (NO3-), nitrite (NO2-), and nitric oxide (NO) have shown numerous beneficial roles through modulating these mechanisms. Therefore, in this review, we highlight the role of reductive pathways of NO formation which lessen the deleterious effects of oxidative damages and increase the adaptation capacity of plants during hypoxia and anoxia. Meanwhile, the overproduction of NO through reductive pathways during hypoxia and anoxia leads to cellular dysfunction and cell death. Thus, its scavenging or inhibition is equally important for plant survival. As plants are also reported to produce a potent greenhouse gas nitrous oxide (N2O) when supplied with NO3- and NO2-, resembling bacterial denitrification, its role during hypoxia and anoxia tolerance is discussed here. We point out that NO reduction to N2O along with the phytoglobin-NO cycle could be the most important NO-scavenging mechanism that would reduce nitro-oxidative stress, thus enhancing plants' survival during O2-limited conditions. Hence, understanding the molecular mechanisms involved in reducing NO toxicity would not only provide insight into its role in plant physiology, but also address the uncertainties seen in the global N2O budget.

Mechanistic Insights of Plant Growth Promoting Bacteria Mediated Drought and Salt Stress Tolerance in Plants for Sustainable Agriculture.

Climate change has devastating effects on plant growth and yield. During ontogenesis, plants are subjected to a variety of abiotic stresses, including drought and salinity, affecting the crop loss (20-50%) and making them vulnerable in terms of survival. These stresses lead to the excessive production of reactive oxygen species (ROS) that damage nucleic acid, proteins, and lipids. Plant growth-promoting bacteria (PGPB) have remarkable capabilities in combating drought and salinity stress and improving plant growth, which enhances the crop productivity and contributes to food security. PGPB inoculation under abiotic stresses promotes plant growth through several modes of actions, such as the production of phytohormones, 1-aminocyclopropane-1-carboxylic acid deaminase, exopolysaccharide, siderophore, hydrogen cyanide, extracellular polymeric substances, volatile organic compounds, modulate antioxidants defense machinery, and abscisic acid, thereby preventing oxidative stress. These bacteria also provide osmotic balance; maintain ion homeostasis; and induce drought and salt-responsive genes, metabolic reprogramming, provide transcriptional changes in ion transporter genes, etc. Therefore, in this review, we summarize the effects of PGPB on drought and salinity stress to mitigate its detrimental effects. Furthermore, we also discuss the mechanistic insights of PGPB towards drought and salinity stress tolerance for sustainable agriculture.

Fe toxicity in plants: Impacts and remediation.

Fe is the fourth abundant element in the earth crust. Fe toxicity is not often discussed in plant science though it causes severe morphological and physiological disorders, including reduced germination percentage, interferes with enzymatic activities, nutritional imbalance, membrane damage, and chloroplast ultrastructure. It also causes severe toxicity to important biomolecules, which leads to ferroptotic cell death and induces structural changes in the photosynthetic apparatus, which results in retardation of carbon metabolism. However, some agronomic practices like soil remediation through chemicals, nutrients, and organic amendments and some breeding and genetic approaches can provide fruitful results in enhancing crop production in Fe-contaminated soils. Some quantitative trait loci have been reported for Fe tolerance in plants but the function of underlying genes is just emerging. Physiological and molecular mechanism of Fe uptake, translocation, toxicity, and remediation techniques are still under experimentation. In this review, the toxic effects of Fe on seed germination, carbon assimilation, water relations, nutrient uptake, oxidative damages, enzymatic activities, and overall plant growth and development have been discussed. The Fe dynamics in soil rhizosphere and role of remediation strategies, that is, biological, physical, and chemical, have also been described. Use of organic amendments, microbe, phytoremediation, and biological strategies is considered to be both cost and environment friendly for the purification of Fe-contaminated soil, while to ensure better crop yield and quality the manipulation of agronomic practices are suggested.

Exogenous melatonin enhances the reactive oxygen species metabolism, antioxidant defense-related gene expression, and photosynthetic capacity of Phaseolus vulgaris L. to confer salt stress tolerance.

Melatonin (MT) has been reported to regulate certain plant physiological processes and promote tolerance to different environmental stresses such as salinity. Green bean (Phaseolus vulgaris L. cv. Royal Nel) seedlings were exposed to 200 mM NaCl with or without pre-treatment with 150 µM MT. Salt stress led to a lower chlorophyll content, a reduced photosynthetic activity, increased reactive oxygen species (ROS) contents, and decreased photosystem II (PSII) activity. The application of exogenous MT to green bean seedlings under salt stress improved photosynthetic activity and alleviated the oxidative damages by enhancing the activity of antioxidant enzymes. The expression of catalase (CAT1), glutathione reductase (GR), superoxide dismutase (CuZnSOD1), ascorbate peroxidase (APX), Peroxiredoxin Q (PrxQ), and 2-cysteine peroxiredoxin (2-Cys-Prx) encoding genes was significantly increased under salt stress in green bean seedling compared with the untreated control. However, plants treated with exogenous MT and NaCl had 28.8, 21.1, 26.1, 20, 26.2, and 22.4% higher CuZnSOD, CAT1, APX, GR, PrxQ, and 2-Cys-Prx transcript levels, respectively, compared to NaCl stress alone. Our study revealed the protective mechanisms mediated by exogenous MT application in NaCl stress alleviation and our findings could be used in the management of green bean cultivation in salinity-prone soils.

Can smart nutrient applications optimize the plant's hidden half to improve drought resistance?

Global agriculture is challenged with achieving sustainable food security while the climate changes and the threat of drought increases. Much of the research attention has focused on above-ground plant responses with an aim to improve drought resistance. The hidden half, that is, the root system belowground, is receiving increasing attention as the interface of the plant with the soil. Because roots are a sensing organ for nutrients and moisture, we speculate that crop root system traits can be managed using smart nutrient application in order to increase drought resistance. Roots are known to be influenced both by their underlying genetics and also by responses to the environment, termed root plasticity. Though very little is known about the combined effect of water and nutrients on root plasticity, we explore the possibilities of root system manipulation by nutrient application. We compare the effects of different water or nutrient levels on root plasticity and its genetic regulation, with a focus on how this may affect drought resistance. We propose four primary mechanisms through which smart nutrient management can optimize root traits for drought resistance: (1) overall plant vigor, (2) increased root allocation, (3) influence specific root traits, and (4) use smart placement and timing to encourage deep rooting. In the longer term, we envision that beneficial root traits, including plasticity, could be bred into efficient varieties and combined with advanced precision management of water and nutrients to achieve agricultural sustainability.

Osmoregulation and its actions during the drought stress in plants.

Drought stress, which causes a decline in quality and quantity of crop yields, has become more accentuated these days due to climatic change. Serious measures need to be taken to increase the tolerance of crop plants to acute drought conditions likely to occur due to global warming. Drought stress causes many physiological and biochemical changes in plants, rendering the maintenance of osmotic adjustment highly crucial. The degree of plant resistance to drought varies with plant species and cultivars, phenological stages of the plant, and the duration of plant exposure to the stress. Osmoregulation in plants under low water potential relies on synthesis and accumulation of osmoprotectants or osmolytes such as soluble proteins, sugars, and sugar alcohols, quaternary ammonium compounds, and amino acids, like proline. This review highlights the role of osmolytes in water-stressed plants and of enzymes entailed in their metabolism. It will be useful, especially for researchers working on the development of drought-resistant crops by using the metabolic-engineering techniques.

Jasmonic acid: a key frontier in conferring abiotic stress tolerance in plants.

Abiotic stresses are the primary sources of crop losses globally. The identification of key mechanisms deployed and established by plants in response to abiotic stresses is necessary for the maintenance of their growth and persistence. Recent discoveries have revealed that phytohormones or plant growth regulators (PGRs), mainly jasmonic acid (JA), have increased our knowledge of hormonal signaling of plants under stressful environments. Jasmonic acid is involved in various physiological and biochemical processes associated with plant growth and development as well as plant defense mechanism against wounding by pathogen and insect attacks. Recent findings suggest that JA can mediate the effect of abiotic stresses and help plants to acclimatize under unfavorable conditions. As a vital PGR, JA contributes in many signal transduction pathways, i.e., gene network, regulatory protein, signaling intermediates and enzymes, proteins, and other molecules that act to defend cells from the harmful effects of various environmental stresses. However, JA does not work as an independent regulator, but acts in a complex signaling pathway along other PGRs. Further, JA can protect and maintain the integrity of plant cells under several stresses by up-regulating the antioxidant defense. In this review, we have documented the biosynthesis and metabolism of JA and its protective role against different abiotic stresses. Further, JA-mediated antioxidant potential and its crosstalk with other PGRs have also been discussed.

5-aminolevulinic acid-mediated plant adaptive responses to abiotic stress.

KEY MESSAGE: 5-aminolevulinic acid (ALA) modulates various defense systems in plants and confers abiotic stress tolerance. Enhancement of crop production is a challenge due to numerous abiotic stresses such as, salinity, drought, temperature, heavy metals, and UV. Plants often face one or more abiotic stresses in their life cycle because of the challenging growing environment which results in reduction of growth and yield. Diverse studies have been conducted to discern suitable mitigation strategies to enhance crop production by minimizing abiotic stress. Exogenous application of different plant growth regulators is a well-renowned approach to ameliorate adverse effects of abiotic stresses on crop plants. Among the numerous plant growth regulators, 5-aminolevulinic acid (ALA) is a novel plant growth regulator, also well-known to alleviate the injurious effects of abiotic stresses in plants. ALA enhances abiotic stress tolerance as well as growth and yield by regulating photosynthetic and antioxidant machineries and nutrient uptake in plants. However, the regulatory roles of ALA in plants under different stresses have not been studied and assembled systematically. Also, ALA-mediated abiotic stress tolerance mechanisms have not been fully elucidated yet. Therefore, this review discusses the role of ALA in crop growth enhancement as well as its ameliorative role in abiotic stress mitigation and also discusses the ALA-mediated abiotic stress tolerance mechanisms and its limitation and future promises for sustainable crop production.

Regulation of Reactive Oxygen Species and Antioxidant Defense in Plants under Salinity.

The generation of oxygen radicals and their derivatives, known as reactive oxygen species, (ROS) is a part of the signaling process in higher plants at lower concentrations, but at higher concentrations, those ROS cause oxidative stress. Salinity-induced osmotic stress and ionic stress trigger the overproduction of ROS and, ultimately, result in oxidative damage to cell organelles and membrane components, and at severe levels, they cause cell and plant death. The antioxidant defense system protects the plant from salt-induced oxidative damage by detoxifying the ROS and also by maintaining the balance of ROS generation under salt stress. Different plant hormones and genes are also associated with the signaling and antioxidant defense system to protect plants when they are exposed to salt stress. Salt-induced ROS overgeneration is one of the major reasons for hampering the morpho-physiological and biochemical activities of plants which can be largely restored through enhancing the antioxidant defense system that detoxifies ROS. In this review, we discuss the salt-induced generation of ROS, oxidative stress and antioxidant defense of plants under salinity.

Effect of tebuconazole and trifloxystrobin on Ceratocystis fimbriata to control black rot of sweet potato: processes of reactive oxygen species generation and antioxidant defense responses.

Black rot, caused by Ceratocystis fimbriata, is one of the most destructive disease of sweet potato worldwide, resulting in significant yield losses. However, a proper management system can increase resistance to this disease. Therefore, this study investigated the potential of using tebuconazole (TEB) and trifloxystrobin (TRI) to improve the antioxidant defense systems in sweet potato as well as the inhibitory effects on the growth of and antioxidant activity in C. fimbriata. Four days after inoculating cut surfaces of sweet potato disks with C. fimbriata, disease development was reduced by different concentrations of TEB + TRI. Infection by C. fimbriata increased the levels of hydrogen peroxide (H2O2), malondialdehyde (MDA), and electrolyte leakage (EL), and the activity of lipoxygenase (LOX) by 138, 152, 73, and 282%, respectively, in sweet potato disks, relative to control. In the sweet potato disks, C. fimbriata reduced the antioxidant enzyme activities as well as the contents of ascorbate (AsA) and reduced glutathione (GSH) by 82 and 91%, respectively, compared with control. However, TEB + TRI reduced the oxidative damage in the C. fimbriata-inoculated sweet potato disks by enhancing the antioxidant defense systems. On the other hand, applying TEB + TRI increased the levels of H2O2, MDA, and EL, and increased the activity of LOX in C. fimbriata, in which the contents of AsA and GSH decreased, and therefore, inhibited the growth of C. fimbriata. These results suggest that TEB + TRI can significantly control black rot disease in sweet potato by inhibiting the growth of C. fimbriata.

Protective role of tebuconazole and trifloxystrobin in wheat (Triticum aestivum L.) under cadmium stress via enhancement of antioxidant defense and glyoxalase systems.

Cadmium (Cd) is a toxic metal and an environmental pollutant that significantly reduces plant growth and productivity. Proper management can ameliorate dysfunction and improve the plant growth and productivity exposed to Cd. Therefore, the present study was conducted to explore the protective role of the fungicides tebuconazole (TEB) and trifloxystrobin (TRI) in helping wheat (Triticum aestivum L. cv. Norin 61) seedlings to tolerate Cd. Five-day-old hydroponically grown seedlings were allowed to mild (0.25 mM CdCl2) and severe (0.5 mM CdCl2) Cd stress separately and with the fungicides (2.75 µM TEB + 1.0 µM TRI) for the next four days. Compared to control, the level of H2O2 in the seedlings exposed to mild and severe Cd stress alone increased by 81 and 112%, respectively. The accumulation of Cd also increased in the wheat seedlings along with declining mineral nutrients under Cd stress. The protective effect of TEB and TRI was observed with the enhancement of the antioxidant defense and methylglyoxalase systems and reduction in oxidative damage. Applying TEB and TRI reduced MDA (by 9 and 18%), EL (by 21 and 17%), MG (by 12 and 17%), and LOX activity (by 37 and 27%), respectively, relative to Cd stress alone. Cadmium uptake also decreased in the shoots (by 48 and 50%, respectively) and roots (by 23 and 25%, respectively) of the fungicide-treated wheat seedlings under mild and severe Cd stress, relative to stress alone. These results indicate the exogenous application of TEB and TRI is a promising approach to improve Cd tolerance in wheat plants. Further investigation is needed under field conditions and for other crop species to determine the Cd-tolerance induced by TEB and TRI application.