Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis.

Feline coronaviruses (FCoV) vary widely in virulence causing a spectrum of clinical manifestations reaching from subclinical course to fatal feline infectious peritonitis (FIP). Independent of virulence variations they are separated into two different types, type I, the original FCoV, and type II, which is closely related to canine coronavirus (CCV). The prevalence of FCoV types in Austrian cat populations without FIP has been surveyed recently indicating that type I infections predominate. The distribution of FCoV types in cats, which had succumbed to FIP, however, was fairly unknown. PCR assays have been developed amplifying parts of the spike protein gene. Type-specific primer pairs were designed, generating PCR products of different sizes. A total of 94 organ pools of cats with histopathologically verified FIP was tested. A clear differentiation was achieved in 74 cats, 86% of them were type I positive, 7% type II positive, and 7% were positive for both types. These findings demonstrate that in FIP cases FCoV type I predominates, too, nonetheless, in 14% of the cases FCoV type II was detected, suggesting its causative involvement in cases of FIP.

Evaluation of three-dimensional ultrasonography of the bovine mammary gland.

OBJECTIVE: To evaluate 3-dimensional (3-D) ultrasonography of the bovine mammary gland, particularly of the teat. ANIMALS: 6 lactating cows. PROCEDURES: Clinical and ultrasonographic examinations of mammary glands of each cow were performed. Teats were removed from a slaughtered cow and examined via ultrasonography. All scans were performed by use of a sensorless standard transducer (8.5 to 10 MHz linear array). The 2-dimensional data acquired were downloaded to an off-line system, and software was used to digitize each image and produce a 3-D block of digitized information. The selected anatomic area was displayed as a 3-D volume cube. RESULTS: Good-quality, 3-D views of the entire mammary gland were acquired by obtaining sections through the glandular parenchyma, gland cistern, teat cistern, and teat canal. CONCLUSIONS AND CLINICAL RELEVANCE: 3-D ultrasonography allows perspective images of the mammary gland to be obtained. Many scanners routinely used in veterinary practice have the resolution required to produce satisfactory images, and the cost of refitting a standard ultrasonographic unit with 3-D software is affordable; however, the cost of a volume transducer with a positioning system and mechanical sweep strategy may be prohibitive. Three-dimensional ultrasonography is a new imaging technique that has promising applications in many fields of veterinary medicine.

Ultrasonography of the bovine udder and teat.

Ultrasonography is a noninvasive technique for examining the bovine udder and teats. It is performed in the standing animal using a high frequency scanner (7.5-10.0 MHz) for examination of the teat structures (teat canal, rosette of Fuerstenberg, teat cistern, gland cistern) and a 5.0 MHz probe for examining the glandular parenchyma. Ultrasonography is a helpful tool to diagnose pathologic alterations of the udder such as inflammation, mucosal lesions, tissue proliferation, foreign bodies, milk stones, congenital changes, hematoma, and abscess. However, ultrasonography of the teat allows for the localization and demarcation of the extent of pathologic changes and therefore is an important additional diagnostic examination technique.