[Intervention effect of narrative therapy on non-suicidal self-injury in adolescents with depressive disorder: a prospective randomized controlled study]. / å™äº‹æ²»ç–—å¯¹é’å°‘å¹´æŠ‘éƒç—‡æ‚£è€ éžè‡ªæ€æ€§è‡ªä¼¤çš„å¹²é¢„æ•ˆæžœï¼šä¸€é¡¹å‰çž»æ€§éšæœºå¯¹ç §ç ”ç©¶.

OBJECTIVES: To study the intervention effect of narrative therapy on non-suicidal self-injury (NSSI), as well as anxiety and depression symptoms in adolescents with depressive disorder. METHODS: Sixty adolescents with depressive disorder and NSSI were randomly assigned to either the intervention group or the control group using coin flipping. The control group received conventional psychological support, while the intervention group received individual narrative therapy in addition to the conventional psychological support (twice a week, 60 minutes per session, for a total of 3 weeks). Assessment of treatment efficacy was conducted using the Adolescent Self-Harm Questionnaire, Children's Depression Inventory, and Children's Anxiety and Mood Scale before the intervention, at the end of the intervention, and one month after the intervention for both groups. RESULTS: A total of 26 adolescents in the intervention group and 29 adolescents in the control group completed the entire study. At the end of the intervention and one month after the intervention, the intervention group showed a significant reduction in the NSSI frequency score, NSSI level, anxiety score, and depression score compared to before the intervention (P<0.017). Moreover, at the end of the intervention and one month after the intervention, the intervention group exhibited significantly lower NSSI frequency score, NSSI severity score, NSSI level, anxiety score and depression score compared to the control group (P<0.05). CONCLUSIONS: Narrative therapy is effective in reducing NSSI frequency and alleviating NSSI severity, as well as anxiety and depression symptoms in adolescents with depressive disorder.

Effectiveness of micro-lecture based cardiac rehabilitation education on health status in individuals with coronary artery disease: A randomized clinical trial.

OBJECTIVE: To explore the effectiveness of the micro-lecture education on the ability of self-management and lifestyle changes in patients with coronary artery disease. DESIGN: A randomized control trial with a 12-week follow-up. SETTING: Cardiology wards of a large Chinese University Hospital. SUBJECTS: One hundred eligible inpatients with coronary artery disease were recruited. INTERVENTION: Intervention group received the micro-lecture education, while the control group received the traditional cardiac rehabilitation education. MAIN MEASURES: Primary outcome of the ability of coronary self-management was assessed by the Coronary self-management scaleat baseline, 12-week after discharge. Secondary outcomes of smoking status, exercise status, and six-minute walk distance were evaluated at the baseline, week4 and week 12 after discharge. The chi-square test,Fisher's exact test, group t-test and the repeated measure variance were used for the data analysis. RESULTS: Eighty-nine of 100 eligible participants responded, and mean age was 60.2 ± 11.3 years.After the intervention, the mean score of the coronary self-management scale (3.84 ± 0.36) was higher than that in the control group (2.78 ± 0.57).At week 12, the rate of re-smoked in the intervention group (6%) was significantly lower than that in the control group (43%),the frequency of the exercise (85%), and the 6MWD (495.55 ± 85.94) in the intervention group were better than the control group. CONCLUSION: Micro-lecture education program presented a positive effect on promoting the ability of self-management, and apotential effect on the therapeutic lifestyle changes for the coronary artery disease patients.

Gene Networks Involved in Plant Heat Stress Response and Tolerance.

Global warming is an environmental problem that cannot be ignored. High temperatures seriously affect the normal growth and development of plants, and threaten the development of agriculture and the distribution and survival of species at risk. Plants have evolved complex but efficient mechanisms for sensing and responding to high temperatures, which involve the activation of numerous functional proteins, regulatory proteins, and non-coding RNAs. These mechanisms consist of large regulatory networks that regulate protein and RNA structure and stability, induce Ca2+ and hormone signal transduction, mediate sucrose and water transport, activate antioxidant defense, and maintain other normal metabolic pathways. This article reviews recent research results on the molecular mechanisms of plant response to high temperatures, highlighting future directions or strategies for promoting plant heat tolerance, thereby helping to identify the regulatory mechanisms of heat stress responses in plants.

New compounds from the seeds of Psoralea corylifolia with their protein tyrosine phosphatase 1B inhibitory activity.

Two new flavonoids, 5,4'-dihydroxy-6,7-furanbavachalcone (1), 1″-methoxy-6,7-furanflavanone (2), together with five known compounds (3-7), were isolated from the seeds of Psoralea corylifolia. Their structures were elucidated by IR, UV, NMR, HRESMS experiments, and comparison of their NMR data with previously reported data. All compounds were evaluated for in vitro inhibitory activity against PTP1B. Additionally, the isolated compounds (1-6) displayed moderate inhibitory effects against PTP1B with IC50 values ranging from 10.3 ± 0.9 to 25.1 ± 1.6 µM.

Mapping genomic hotspots of DNA damage by a single-strand-DNA-compatible and strand-specific ChIP-seq method.

Spontaneous DNA damage may occur nonrandomly in the genome, especially when genome maintenance mechanisms are undermined. We developed single-strand DNA (ssDNA)-associated protein immunoprecipitation followed by sequencing (SPI-seq) to map genomic hotspots of DNA damage. We demonstrated this method with Rad52, a homologous recombination repair protein, which binds to ssDNA formed at DNA lesions. SPI-seq faithfully detected, in fission yeast, Rad52 enrichment at artificially induced double-strand breaks (DSBs) as well as endogenously programmed DSBs for mating-type switching. Applying Rad52 SPI-seq to fission yeast mutants defective in DNA helicase Pfh1 or histone H3K56 deacetylase Hst4, led to global views of DNA lesion hotspots emerging in these mutants. We also found serendipitously that histone dosage aberration can activate retrotransposon Tf2 and cause the accumulation of a Tf2 cDNA species bound by Rad52. SPI-seq should be widely applicable for mapping sites of DNA damage and uncovering the causes of genome instability.

Cellulose nanofiber/MXene/luffa aerogel for all-weather and high-efficiency cleanup of crude oil spills.

The remediation of heavy crude oil spills is a global challenge because frequent crude oil spills cause long-term damage to local living beings and marine ecosystems. Herein, a solar-driven and Joule-driven self-heated aerogel were developed as an all-weather adsorbent to efficiently absorb crude oil by obviously decreasing the viscosity of crude oil. The cellulose nanofiber (CNF)/MXene/luffa (CML) aerogel was fabricated via a simple freeze-drying method using CNF, MXene, and luffa as raw materials, and then coated with a layer of polydimethylsiloxane (PDMS) to make it hydrophobic and further increase oil-water selectivity. The aerogel can quickly reach 98 °C under 1 sun (1.0 kW/m2), which remains saturated temperature after 5 times photothermal heating/cooling cycles, indicating that the aerogel has great photothermal conversation capability and stability. Meanwhile, the aerogel can also rapidly rise to 110.8 °C with a voltage of 12 V. More importantly, the aerogel achieved the highest temperature of 87.2 °C under outdoor natural sunlight, providing a possibility for promising applications in practical situations. The remarkable heating capability enables the aerogel to decrease the viscosity of crude oil substantially and increase the absorption rate of crude oil by the physical capillary action. The proposed all-weather aerogel design provides a sustainable and promising solution for cleaning up crude oil spills.

Multifunctional MXene Conductive Zwitterionic Hydrogel for Flexible Wearable Sensors and Arrays.

Conductive hydrogels have good prospects in the fields of flexible electronic devices and artificial intelligence due to their biocompatibility, durability, and functional diversity. However, the process of hydrogel polymerization is time-consuming and energy-consuming, and freezing at zero temperature is inevitable, which seriously hinders its applications and working life. Herein, zwitterionic conductive hydrogels with self-adhesive and antifreeze properties were prepared in one minute by introducing two-dimensional (2D) MXene nanosheets into the autocatalytically enhanced system composed of tannic acid-modified cellulose nanofibers and zinc chloride. The system has strong environmental applicability (-60 to 40 °C), good stretchability (ductility ≈ 980%), durable adhesion (even after 30 days of exposure to air), and strong electrical conductivity (20 °C, 30 mS cm-1). By virtue of these advantages, the prepared zwitterionic hydrogels can be developed into flexible strain sensors to monitor large human movements and subtle physiological signals over a wide temperature range and to capture signals from handwriting and voice recognition. In addition, multiple flexible sensors can be assembled into a three-dimensional (3D) array, which can detect the magnitude and spatial distribution of strain or force. These results demonstrate that the prepared zwitterionic hydrogels have promising applications in the fields of medical monitoring and artificial intelligence.

Cellulose nanofiber/molybdenum disulfide aerogels for ultrahigh photothermal effect.

The photothermal materials have a broad range of applications in crude oil spills treatment, desalination, and photothermal therapy. However, the rational construction of aerogels with exceptional photothermal performance is highly desired yet still challenging. Herein, a class of stable aerogels comprised of molybdenum disulfide (MoS2) nanoflowers and cellulose nanofibers (CNFs) was fabricated, affording extraordinary light-to-heat energy conversion capability. Benefiting from the intercalated porous structure, the resultant cellulose nanofibers/molybdenum disulfide (CNF/MoS2) aerogels deliver an ultrahigh temperature output up to 260.4 °C with near infrared (NIR) laser power densities of 0.8 W cm-2. Remarkably, when NIR laser power density increased to 1.0 W cm-2, the aerogels began to burn, achieving the superhigh surface temperature of âˆ¼ 690 °C. The combustion process of CNF/MoS2 composite aerogels was evaluated in detail. Therefore, this work provides experiment evidence and theoretical basis for the rational applications of photothermal materials at high temperature in future.

Multifunctional bacterial cellulose-based organohydrogels with long-term environmental stability.

Sensitive strain sensors have attracted more attention due to their applications in health monitoring and human-computer interaction. However, the problems existing in conventional hydrogels, such as inherent brittleness, freezing at low temperature, low toughness, and water evaporation, have greatly hindered the practical applications. In order to solve the above problems, herein, we designed dual network multifunctionality organohydrogels using polyvinylpyrrolidone (PVP) and polyvinyl alcohol (PVA) covalent cross-linking polymer as the first network, the bacterial celluloses (BCs) and calcium chloride by ligand binding as the second network. The prepared organohydrogels showed good conductivity and sensitivity over a wide temperature range (-20 âˆ¼ 40 ℃), and maintained long-term stability (>15 days) in the air. In addition, the dynamic combination of BCs-Ca2 + and hydrogen bonds in the binary system further endows the organohydrogels with excellent tensile strength (≈1.0 MPa), tensile strain (≈1300%), toughness (≈6.2 MJ m-3), conductivity (3.4 S m-1), gauge factor (≈1.24), adhesion (≈0.3 MPa), and self-healing properties (self-healing tensile strain to 632%). Therefore, this organohydrogel has potential candidates for flexible electronic skin, motion monitoring, and soft robotics.

Genotype association of C(-735)T polymorphism of the MMP-2 gene with the risk of carotid atherosclerosis-vulnerable plaque in the Han Chinese population.

The aim of the current study was to explore the possible association of the polymorphism of C(-735)T in MMP-2 with the vulnerable plaque risk in ultrasound-confirmed carotid atherosclerosis cases. Serum MMP-2 levels were measured to investigate the relationship between the MMP-2 level and the genetic variability. The MMP-2 polymorphism was detected by PCR-RFLP in the 243 cases with stable plaque and 221 cases with vulnerable plaque. Serum MMP-2 levels were measured with ELISA. The results showed that MMP-2 was significantly higher in the cases with vulnerable plaque than in the cases with stable plaque. A statistical difference was found between the genotype distributions in the vulnerable plaque cases and that in the stable cases. T-allele frequency was also found to be over-represented in the stable plaque cases than in the vulnerable plaque cases, which might partially explain the observed difference in the serum MMP-2 levels in the different plaque cases. The current results also suggested that MMP-2 was a risk factor in the cases with vulnerable plaques, whereas TT genotype and T allele might be protective factors in the cases with vulnerable plaques.

Recent Developments and Applications of Hemicellulose From Wheat Straw: A Review.

Hemicellulose is an important component of plant cell walls, which is mainly used in biofuels and bioproducts. The hemicellulose extracted from different plant sources and plant locations has different microstructure and molecule. Wheat straw is an important biomass raw material for the extraction of hemicellulose. The aims of this review are to summary the recent developments and various applications of hemicellulose from wheat straw. The microstructure and molecule of hemicellulose extracted by different methods are comparably discussed. The hemicellulose-based derivatives and composites are also reviewed. Special attention was paid to the applications of hemicellulose such as biofuel production, packaging field, and adsorbent. The problems and developing direction were given based on our knowledge. We expect that this review will put forward to the development and high-value applications of hemicellulose from wheat straw.

Adsorption of lead(II) from aqueous solution onto Hydrilla verticillata.

The adsorption of Pb(II) onto Hydrilla verticillata was examined in aqueous solution with parameters of pH, adsorbent dosage, contact time and temperature. The linear Langmuir and Freundlich models were applied to describe equilibrium isotherms, and both models fitted well. The monolayer adsorption capacity of Pb(II) was found as 104.2 mg/g at pH 4 and 25 degrees C. Dubinin-Radushkevich (D-R) isotherm model was also applied to the equilibrium data. The mean free energy of adsorption (15.81 kJ/mol) indicated that the adsorption of Pb(II) onto H. verticillata may be carried out via chemical ion-exchange mechanism. Thermodynamic parameters, free energy (DeltaG (0)), enthalpy (DeltaH (0)) and entropy (DeltaS (0)) of adsorption were also calculated. These parameters showed that the adsorption of Pb(II) onto H. verticillata was a feasible, spontaneous and exothermic process in nature. The influence of Cd(2+), Cu(2+) and Ni(2+) on adsorption of Pb(2+) onto H. verticillata was studied, too. In the investigated range of operating conditions, it was found that the existence of Cd (2+), Cu (2+) and Ni (2+) had no impact on the adsorption of Pb(2+).

Superoxide dismutase activity and malondialdehyde level in plasma and morphological evaluation of acute severe hemorrhagic shock in rats.

OBJECTIVES: The aim of the study was to investigate the changes of the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in plasma and organ damage during the acute severe hemorrhagic shock (ASHS), as well as to analyze their relationship. METHODS: Twenty male Wistar rats (230-270 g) were randomly divided into sham hemorrhage shock (SHS) group and ASHS group. Acute severe hemorrhagic shock rats were induced by drawing blood through a femoral arterial catheter for 15 minutes with the final mean arterial blood pressure decreased to 35 to 40 mm Hg. The animals were killed after the mean arterial blood pressure was maintained at this level for 1 hour. The activity of SOD and the level of MDA in plasma were measured, and pathologic changes of the major organs (heart, liver, spleen, lung, kidney, and brain) were observed by microscopy. RESULTS: The SOD activities and MDA levels in the ASHS group both increased continuously during the whole experiment. The SOD activities and MDA levels in plasma were not significantly different between the prehemorrhagic stage of ASHS and that of SHS (P > .05). The SOD activities and MDA levels were significantly higher in the ASHS initial stage than in the prehemorrhagic stage (P < .01). Compared with the ASHS initial stage, there was a significant (P < .01) increase in SOD activities and MDA levels in the ASHS end stage. Severe microscopic injuries appeared in the major organs (heart, liver, spleen, lung, kidney, and brain) of ASHS rats. CONCLUSION: The changes of the activity of SOD and the level of MDA in ASHS may have a positive correlation.

[CD147 increases invasiveness of U937 cells through regulation of matrix metalloproteinase activity].

This study was purpose to investigate the effects of CD147 on the invasiveness of leukemia cells U937. The experiments were divided into 4 groups: control group, LPS group, CD147mAb group and LPS+CD147 mAb group. Cells were treated by lipopolysaccharide (LPS) or anti-CD147 monoclonal antibody, and the expression of CD147 and MMP-2, -9, the invasive potential of the cells in vitro and ex vivo, as well as the invasion of the implanted tumors in SCID mice were analysed by RT-PCR, FCM, gel zymography and invasion test in vitro respectively. The results showed that the expression of CD147 was elevated by the induction of LPS, and the enhanced expression of CD147 on U937 cells increased the production and secretion of MMP-2 and MMP-9 as measured by reverse transcription-PCR and gel zymography. An increased number of LPS-induced cells invading through a reconstituted basement membrane were observed by invasion assays. These responses were down-regulated after blocking CD147 with anti-CD147 antibody. At 30 days after intravenous injection of LPS pretreated U937 cells to SCID mice human U937 cells were found in the bone marrow and lung of the mice, indicating the invasion of the tumor cells. And overexpressions of CD147, MMP-2 and MMP-9 were found in the lung tissue of the mice injected with LPS-treated but not anti-CD147 antibody treated tumor cells. It is concluded that overexpression of CD147 on U937 cells may increase the secretion and activation of MMP-2 and MMP-9 and thus promote the invasiveness of the tumor cells.