RESUMO
We utilized the reaction of chitosan with acetic anhydride to form a chitin gel. This gel was then dried, which formed a chitin sheet. The procedure was extremely easy for a biologist unfamiliar with materials engineering. Spheroids derived from HEK293T cells were formed on the chitin sheet, because the spheroids slightly attached and slowly moved on the chitin sheet.
Assuntos
Adesão Celular , Quitina/metabolismo , Esferoides Celulares/metabolismo , Anidridos Acéticos/metabolismo , Animais , Movimento Celular/efeitos dos fármacos , Quitosana/metabolismo , Técnicas de Cocultura , Meios de Cultura/química , Fibroblastos/metabolismo , Gelatina/farmacologia , Géis/metabolismo , Células HEK293 , Humanos , Laboratórios , Camundongos , Propriedades de Superfície , Viscosidade/efeitos dos fármacosRESUMO
We fabricated uniform straw-like cell architecture with central lumen using a suture thread within 1 h. The architecture consisting of cancer cells and mature adipocyte was used for cell-cell communication assay, although mature adipocyte could not form spontaneous multi-cellular spheroids. Using the system, it is possible to investigate three-dimensional cell-cell communication as an alternative to animal experiments. ABBREVIATIONS: EB: embryoid body; 2D: two-dimension; 3D: three-dimension; PBS: phosphate buffered saline.