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1.
Parasitol Res ; 118(1): 43-46, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30315356

RESUMO

Despite the wide distribution of wild boar populations in Italy and the increase of its diffusion in urbanized areas, only one case report has described the occurrence of Echinococcus granulosus s.l. in a wild boar from Marche (Central Italy). The present study investigated the presence of E. granulosus sensu lato with an epidemiological survey on wild boars from central Italy that had been killed during hunting season. Seven hundred sixty-five (765) adult wild boars were examined during the 2016-2017 hunting season. Of these animals, 1.0% (8/765) were positive to E. granulosus s.l. with a fertility of 0.3% (2/765), and 2.9% animals (22/765) were positive for the metacestode stage of Taenia hydatigena (Cysticercus tenuicollis), while 0.5% (4/765) showed mixed infection (E. granulosus s.l. + T. hydatigena). Sixteen hydatids were found, of which 12.5% were fertile, 37.5% were sterile, 31.3% were caseous, and 18.8% were calcified. Eight hydatids (two fertile and six sterile cysts) were molecularly characterized by analysis of the mitochondrial gene, cytochrome c oxidase subunit 1 (cox1), and the NADH dehydrogenase subunit 1 (ND1). Hydatids found in wild boars were characterized as E. granulosus sensu stricto (G1 genotype). The present survey represents the first epidemiological study on cystic echinococcosis in wild boar in Italy which highlights the need for more extensive epidemiological investigations to determine the causal factors, economic impact, and public health importance of the disease in this livestock-wildlife setting.


Assuntos
Coinfecção/veterinária , Equinococose/veterinária , Echinococcus granulosus/isolamento & purificação , Sus scrofa/parasitologia , Doenças dos Suínos/epidemiologia , Animais , Coinfecção/epidemiologia , Coinfecção/parasitologia , Cistos , Equinococose/epidemiologia , Equinococose/parasitologia , Echinococcus granulosus/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Genótipo , Itália/epidemiologia , Proteínas Mitocondriais/genética , Inquéritos e Questionários , Suínos , Doenças dos Suínos/parasitologia
2.
Parasitol Res ; 116(4): 1227-1235, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28197762

RESUMO

The lungworm Aelurostrongylus abstrusus is the most important respiratory parasite of domestic cats. Pulmonary aelurostrongylosis has been reported in wild felids, though unequivocally evidence of wildlife infection by A. abstrusus is scant. Recently, Troglostrongylus brevior, a lungworm usually infecting wild felids, has been described in domestic cats from Mediterranean areas. The present work evaluates the sequence variation of an informative region within the gene encoding the mitochondrial cytochrome c oxidase subunit 1 of A. abstrusus and T. brevior, in order to provide novel information on the genetic make-up of these lungworms. Parasitic stages of A. abstrusus and T. brevior were collected from domestic and wild hosts (i.e., domestic cat, European wildcat, caracal, serval, and lion) from Italy, Greece, and South Africa. Five (HI-HV) and four (HI-HIV) haplotypes were recorded for A. abstrusus and T. brevior, respectively, mostly shared between domestic and wild felids in different geographical areas. The phylogenetic analysis showed that all haplotypes of A. abstrusus and T. brevior clustered as monophyletic groups with a strong nodal support, indicating that all haplotypes identified were distinct from each other. All sequence types represent two distinct species, A. abstrusus and T. brevior, and these genetic convergences are also detected within and among populations of these nematodes, irrespective of their hosts and geographical origin. The occurrence of A. abstrusus and T. brevior haplotypes in different hosts from the same regions and between different countries indicates that the same lungworm populations circulate in domestic and wild hosts under the same routes of transmission.


Assuntos
Felidae/parasitologia , Haplótipos , Metastrongyloidea/genética , Infecções por Strongylida/veterinária , Animais , Animais Domésticos , Animais Selvagens , Grécia/epidemiologia , Itália/epidemiologia , Filogenia , África do Sul/epidemiologia , Infecções por Strongylida/epidemiologia , Infecções por Strongylida/parasitologia
3.
Exp Parasitol ; 164: 31-5, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26873272

RESUMO

Despite the importance of Neospora caninum in veterinary medicine, knowledge of distribution of neosporosis in dog populations in some countries is still poor. The aims of the present study were to determine the occurrence of anti-N. caninum antibodies in one-hundred dogs living in cattle farms or dog breedings in central Italy and to evaluate the risk factors associated with seropositivity. The incidence of reproductive system disorders (e.g. infertility after first pregnancy) was also evaluated. Serum from breeding and farm dogs was tested to an indirect immunofluorescent antibody test (IFAT) to assess the occurrence of seropositivity. Management and individual data were collected and analysed both by linear and logistic multiple-regression models to find reliable predictors of seroprevalence and anti-N. caninum antibody level. The seropositivity for N. caninum was 32%. Dogs reared for breeding and presence of cattle on the farm were associated with seropositivity for N. caninum. Dogs living in the cattle farms showed a higher seropositivity for N. caninum (46%) compared with those living in dogs breeding (18%) (P < 0.05). The high presence of seropositive dogs in cattle farms of the study region demonstrates the potential risk of horizontal transmission of N. caninum between dogs and cattle, regardless the occurrence of reproductive system disorders or with infectious bovine tissues contact. Although the Neospora seropositivity in dog breedings may appear relatively low if compared with that found in dogs living with livestock, this infection, apparently underestimated, should be considered as a potential serious problem in canine medicine.


Assuntos
Anticorpos Antiprotozoários/sangue , Coccidiose/veterinária , Doenças do Cão/epidemiologia , Neospora/imunologia , Animais , Cruzamento , Bovinos , Doenças dos Bovinos/parasitologia , Doenças dos Bovinos/transmissão , Coccidiose/complicações , Coccidiose/epidemiologia , Coccidiose/imunologia , Doenças do Cão/imunologia , Doenças do Cão/parasitologia , Cães , Feminino , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Masculino , Análise Multivariada , Gravidez , Complicações Parasitárias na Gravidez/epidemiologia , Complicações Parasitárias na Gravidez/parasitologia , Complicações Parasitárias na Gravidez/veterinária , Fatores de Risco , Sensibilidade e Especificidade , Estudos Soroepidemiológicos
4.
Parasitol Res ; 115(10): 3731-5, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27230016

RESUMO

The increasing interest on respiratory nematodes of domestic cats (Felis silvestris catus) has recently stimulated several studies on their epidemiology and geographic distribution. At the same time, there are still important gaps in our knowledge of the infections caused by respiratory nematodes in wild felids. The present study investigated the occurrence of pulmonary parasites in wild hosts housed in sanctuaries and protected areas of South Africa. Faecal samples collected from seven species of wild felids living in three study sites were copromicroscopically and genetically examined. Of twenty-one samples six, i.e. three from caracals (Caracal caracal), two from lions (Panthera leo) and one from a serval (Leptailurus serval), scored positive for the metastrongyloid Aelurostrongylus abstrusus at copromicroscopic and/or molecular tests. No other lungworms were recorded. The occurrence of the cosmopolitan cat lungworm A. abstrusus in wild hosts has been so far questioned. Importantly, the present findings represent an unequivocal evidence of the capability of A. abstrusus to infect some species of wild felids. Further studies are warranted to understand the epidemiological patterns of lungworms in wild and domestic felids, and to better investigate the impact of these parasitoses on health and welfare of wild animals.


Assuntos
Doenças do Gato/parasitologia , Felidae/parasitologia , Metastrongyloidea/isolamento & purificação , Infecções por Strongylida/veterinária , Animais , Doenças do Gato/epidemiologia , Gatos , Fezes/parasitologia , Feminino , Leões/parasitologia , Masculino , África do Sul/epidemiologia , Infecções por Strongylida/epidemiologia , Infecções por Strongylida/parasitologia
5.
J Clin Microbiol ; 53(9): 3009-13, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26109447

RESUMO

Feline lungworms and heartworms are stimulating the interest of the scientific community due to their clinical impact and apparent geographical expansion. Diagnosis of the infections caused by these nematodes is indeed challenging. This report describes a novel multiplex PCR able to identify simultaneously three species of lungworms (Aelurostrongylus abstrusus and Troglostrongylus brevior) and heartworms (Angiostrongylus chabaudi) affecting felids. Epidemiological and clinical perspectives are discussed.


Assuntos
Doenças do Gato/diagnóstico , Helmintíase Animal/diagnóstico , Metastrongyloidea/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase Multiplex/métodos , Medicina Veterinária/métodos , Animais , Doenças do Gato/parasitologia , Gatos , Helmintíase Animal/parasitologia , Metastrongyloidea/genética
6.
Parasitol Res ; 114(10): 3649-55, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26122991

RESUMO

The metastrongyloid nematode Angiostrongylus vasorum affects the heart and pulmonary arteries of dogs and wild animals. Over the recent years, dog angiostrongylosis has gained great attention in the veterinary community for the expansion of its geographic range and for a rise in the number of clinical cases. Global warming, changes in phenology of mollusc intermediate hosts and movements of wild reservoirs have been evocated in the spreading of mollusc-borne parasites, including A. vasorum. The land snail Helix aspersa, a vector of other respiratory metastrongyloids, is endemic in most regions of the World, where it is a pest outside its native Mediterranean range. In the present study, the susceptibility and suitability of H. aspersa as an intermediate host of A. vasorum were investigated along with the characteristics of larval recovery and development following two different ways of inoculation, i.e. experimental (group A) vs natural infection (group B). After infections, the snails were kept at environmental conditions for 2 months. Five snails from groups A and B were randomly selected, digested and examined at 15-day intervals for 2 months. L1s, L2s and L3s were microscopically identified based on key morphological and morphometric characteristics and their identity was genetically confirmed. The results showed that A. vasorum may reach the infective stage in H. aspersa and that uptake of larvae and parasitic burden within the snails depend on the grazing capability of the molluscs. Biological and epidemiological implications are discussed.


Assuntos
Angiostrongylus/fisiologia , Caramujos/parasitologia , Animais , Interações Hospedeiro-Parasita , Larva/fisiologia , Masculino
7.
Parasitol Res ; 114(6): 2135-41, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25773179

RESUMO

The present study evaluated the occurrence of zoonotic parasites in feces and on fur of stray and private dogs living in Italy. Individual fecal samples collected from 117 and 385 kenneled (i.e., rescue shelter) and privately owned dogs, respectively, were examined by conventional copromicroscopy. Samples positive for Giardia duodenalis and Cryptosporidium spp. were subjected to further genetic characterization. Additionally, 174 fur samples of private and kenneled dogs living in the same area, 27 of which were also subjected to copromicroscopical examinations, were examined for the presence and viability of parasite elements. Out of 502 fecal samples, forty-one (8.2%) scored positive for G. duodenalis cysts while one (0.2%) for Cryptosporidium oocysts. Nine private dogs were molecularly positive for G. duodenalis assemblage C (n. 7), assemblage D (n. 1), and assemblages C + D (n. 1), while 11 kenneled dogs were positive for assemblage C (n. 8), assemblage D (n. 1), and assemblages C + D (n. 2). Cryptosporidium spp. were identified as C. canis in a private dog. One hundred and seventy-two dogs (34.3%), i.e., 92 private and 80 kenneled, were positive for zoonotic nematodes: Toxocara canis (6.6 %), Ancylostoma caninum (6.8%), and Capillaria aerophila (0.6%). Unviable eggs of Toxocara spp. were found on the hair of five (2.9%) private dogs. The present findings indicate that canine feces from both private and kenneled animals may contain zoonotic parasites and may be a potential risk for humans and other animals, especially when they contaminate the environment. The role of dog fur as source of human infections should be further investigated.


Assuntos
Doenças do Cão/parasitologia , Cabelo/parasitologia , Helmintíase Animal/parasitologia , Oocistos , Infecções Protozoárias em Animais/parasitologia , Zoonoses/parasitologia , Animais , Doenças do Cão/epidemiologia , Cães , Fezes/parasitologia , Helmintíase Animal/epidemiologia , Abrigo para Animais , Humanos , Itália/epidemiologia , Propriedade , Prevalência , Infecções Protozoárias em Animais/epidemiologia
8.
Parasitol Res ; 113(2): 613-8, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24271082

RESUMO

Parasitic nematodes affecting the respiratory system of felids are spreading in endemic regions and emerging in previously free areas and/or hosts. This is particularly the case of the cat lungworm Aelurostrongylus abstrusus, which can cause respiratory signs in cats all over the world. Additionally, Troglostrongylus brevior has been recently found in domestic cats from Ibiza Island in Spain and Southern Italy. The present paper describes the first mixed infection by these lungworms in kittens belonging to the same litter. Two ∼10-11 weeks old kittens were found infected by A. abstrusus and T. brevior at a copromicroscopical examination. The identity of larvae shed by faeces were confirmed with an already validated PCR specific for A. abstrusus and a novel DNA-based assay specific for T. brevior. One kitten showed severe respiratory signs and died despite an anthelmintic treatment, while the other had a subclinical infection and recovered after a parasiticide administration with milbemycin oxime. New insights into epidemiology, biology, clinical aspects and control of these parasitoses are discussed.


Assuntos
Doenças do Gato/parasitologia , Pneumopatias Parasitárias/veterinária , Metastrongyloidea/isolamento & purificação , Infecções por Strongylida/veterinária , Animais , Gatos , Coinfecção/parasitologia , Coinfecção/veterinária , Fezes/parasitologia , Feminino , Itália , Larva/anatomia & histologia , Pneumopatias Parasitárias/parasitologia , Masculino , Metastrongyloidea/anatomia & histologia , Reação em Cadeia da Polimerase/veterinária , Infecções por Strongylida/parasitologia
9.
Ann Parasitol ; 70(2): 113-118, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39154198

RESUMO

Duddingtonia flagrans is a nematode trapping fungus used for the control of gastrointestinal nematodes in livestock. The quantity of chlamydospores of D. flagrans required for the reduction of third-stage larvae (L3) of sheep gastrointestinal nematodes (GIN) is largely unknown, and a matter of discussion. The aim of this experiment was to determine in vitro the nematophagous activity of four different concentrations of D. flagrans (1000, 3000, 6250, or 11000 chlamydospores/ml) in the presence of varying numbers of GIN third-stage larvae (L3) (500, 1000, 1500). Additionally, the study sought to evaluate the efficacy of this fungus on Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis and Chabertia ovina. The results showed that as fungal concentrations increased, so did the larval reduction of third-stage infective larvae in each test. L3s number was not a determining factor in the efficacy against GIN. The comparison between various concentrations of chlamydospores revealed significant differences, particularly between 1000 and 11000 chlamydospores (P≤0.05). Regarding the larval reduction of the GIN species considered, D. flagrans demonstrated the same effectiveness across all species tested. The results of the current study confirm the efficacy and underscore the importance of D. flagrans as an alternative for controlling of GIN.


Assuntos
Ascomicetos , Nematoides , Infecções por Nematoides , Doenças dos Ovinos , Animais , Ovinos , Projetos Piloto , Doenças dos Ovinos/parasitologia , Doenças dos Ovinos/prevenção & controle , Infecções por Nematoides/veterinária , Infecções por Nematoides/parasitologia , Infecções por Nematoides/prevenção & controle , Ascomicetos/fisiologia , Larva , Controle Biológico de Vetores/métodos , Duddingtonia/fisiologia
10.
Vet Parasitol ; 331: 110271, 2024 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-39089177

RESUMO

The metastrongyloids Aelurostrongylus abstrusus and Troglostrongylus brevior are primary causes of feline clinical respiratory disease. The present field trial evaluated the clinical efficacy of a spot-on formulation containing eprinomectin, esafoxolaner and praziquantel (NexGard® Combo) administered per label recommendations to cats affected with aelurostrongylosis and/or troglostrongylosis. Overall, 36 naturally infected cats were randomly assigned to Group 1 (G1) or Group 2 (G2) of 18 cats each. The two groups included 6 cats with A. abstrusus, T. brevior and mixed infection, each. All cats completed the study. Cats in G1 were treated on study Days (SDs) 0 and 28±2. Cats in G2 served as negative control until SD 56±2 and were then treated on SD 56±2 and 84±2. On SD 0/-7, 28±2 and SD 56±2 all cats were subjected to parasitological (quali-quantitative Baermann) and clinical examinations (physical exams and thoracic X-rays). Hematology and biochemistry analyses were performed on SD 0/-7 and SD 56±2. On SD 84±2 quali-quantitative Baermann, clinical examination and thorax radiography were performed on all G2 cats and on two G1 cats that still had radiographic alterations on SD 56±2. On SD 112±2 all G2 cats were subjected to parasitological and clinical evaluations and one cat from G1 that still had radiographic signs at SD 84±2 was clinically and radiographically evaluated. Efficacy criteria were the reduction of larval shedding in faeces and the clinical response in terms of pathological and radiographic scores after treatment compared to the baseline. An efficacy of 100 % based on LPG reduction was recorded after one (20/24 cats) or two (all 24 cats) treatments in cats with single infection by A. abstrusus or T. brevior. For cats with mixed infections, larval shedding was stopped after one (11/12 cats) or two (all 12 cats) treatments. Statistically significant clinical and radiographic improvement was evident in all study cats after 2 treatments. The present data show that two monthly treatments with NexGard® Combo stopped larval shedding and led to a significant clinical recovery and a complete resolution of radiographic abnormalities in cats infected with A. abstrusus and/or T. brevior.

11.
Vet Parasitol ; 331: 110274, 2024 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-39116546

RESUMO

The tapeworm Dipylidium caninum is the most widely distributed cestode infecting dogs, cats, and sometimes humans, worldwide. The diagnosis of the infection caused by D. caninum is achieved via the visualization of proglottids in feces or with traditional microscopic tests, but both lack sensitivity. The present study has evaluated and compared the diagnostic performance of a PCR protocol on different feline biological samples to detect D. caninum. A sample of feces, a Scotch tape test from the perianal area, and a rectal swab were collected from a total of 100 privately owned cats from Italy and Greece. All fecal samples were subjected to macroscopic examination and to floatation. Based on the results of the above tests the cats were divided in three groups, i.e. (i) cats positive for D. caninum (regardless of positivity for other endoparasites (Group A; n = 50 cats), (ii) cats negative for D. caninum but infected by other helminths (Group B; n = 25 cats), and (iii) cats negative for intestinal endoparasites (Group C; n = 25 cats). For each sample, the DNA was extracted from feces, floatation supernatant, Scotch tape test and rectal swabs and subjected to PCR. For 33 cats from Group A, at least one sample type scored positive at PCR. Of these, all were PCR-positive in the floatation aliquot, while nine and one cats were positive by PCR on feces and Scotch tape test, respectively. Swabs were negative by PCR for all the cats. None of the samples from cats of Groups B and C was positive by any PCR. Sequences obtained from amplicons generated from samples of cats enrolled in Italy had 99-100 % identity with the recently described D. caninum feline genotype. The data presented here suggest that PCR could be a useful tool for diagnosing D. caninum infections, under certain circumstances, e.g. when proglottids are unidentified, unseen or overlooked, even though it has limitations, e.g. false negative results due to fecal PCR inhibitors, uneven distribution of parasitic elements, or to intermittent proglottid and/or egg shedding. Thus, it may not be, currently, the best diagnostic choice for dipylidiosis.

12.
Artigo em Inglês | MEDLINE | ID: mdl-39139660

RESUMO

Feline hepatozoonosis is a vector-borne disease caused by different species of the genus Hepatozoon, i.e. Hepatozoon felis, Hepatozoon silvestris and Hepatozoon canis. Knowledge on the biology, epidemiology and taxonomy of Hepatozoon spp. is still limited, despite the fact that the number of documented Hepatozoon spp. infections in domestic cats increased in recent years in different countries. This study was carried out to evaluate the prevalence and the genetic profile of Hepatozoon spp. in cats living on the island of Skopelos, Greece. Individual blood samples were collected from 54 owned cats and were subjected to Giemsa-stained blood smear examination to investigate the presence of Hepatozoon spp. gamonts and to a specific PCR protocol targeting the 18S rRNA gene of Hepatozoon. A total of 45 cats (83.3%) were found infected by Hepatozoon spp. by at least one of the methods applied. In particular, 43 (79.6%) of the cats were PCR-positive, and in 6 (11.1%) cats gamonts of Hepatozoon spp. were found in the blood smears. A total of 26 H. felis sequences were obtained and the presence of three undescribed single nucleotide polymorphisms were detected. The present results indicate that H. felis species complex may be hyperendemic in isolated/confined areas. In such contexts, geographical isolation may favor the origin of new genotypes or haplotypes or even new species.

13.
Vet Parasitol Reg Stud Reports ; 29: 100689, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35256130

RESUMO

Intestinal helminthes of dogs have a relevant role for health and welfare of canine populations and many of them are zoonotic. Thus, a constant surveillance is pivotal to safeguard companion dogs and human health. Data from faecal examinations of pet dogs obtained in the last six years during the routine activity of the laboratory of Parasitology and Parasitic diseases of the University Teaching Veterinary Hospital of the University of Teramo, Italy, are here reported. From 2015 to 2020 a total of 1083 individual faecal samples from privately owned dogs were examined using conventional copro-microscopic techniques. Endoparasite elements were found in 278 (25.7%) dogs, and among them, eggs of intestinal zoonotic helminthes were detected in 184 (17%) samples. The most common zoonotic parasites were Toxocara canis and hookworms (8.9% positivity rate for both). Most dogs (88%) were positive for a single zoonotic parasite while 12% of the positive animals harbored two or more zoonotic helminthes. A statistical analysis showed a significant association between dogs with less than one year of age and positivity to at least one intestinal zoonotic helminth, and that infections by T. canis are significantly more prevalent in dogs under one year of age. Despite being well known in canine clinical practice, these results indicate that zoonotic helminthes are still frequent in owned dogs. Thus, increased awareness of veterinary practitioners and pet owners is necessary to implement current diagnostic and control strategies to minimize the risk of zoonotic transmission of intestinal helminthes.


Assuntos
Doenças do Cão , Helmintos , Enteropatias Parasitárias , Parasitos , Infecções por Trematódeos , Animais , Doenças do Cão/diagnóstico , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Cães , Fezes/parasitologia , Helmintíase , Humanos , Enteropatias Parasitárias/diagnóstico , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/veterinária , Infecções por Trematódeos/veterinária
14.
Animals (Basel) ; 12(8)2022 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-35454269

RESUMO

The gold standard method for the diagnosis of cat aelurostrongylosis is the detection of Aelurostrongylus abstrusus first stage larvae with the Baermann's examination. Nevertheless, molecular assays have shown higher diagnostic performances compared to copromicroscopy. This study evaluated the usefulness of an A. abstrusus species-specific PCR on different biological samples collected in clinical settings from 100 privately-owned cats in Italy (n. 60) and Greece (n. 40). A fecal sample was collected from each animal and a pharyngeal swab was also obtained for cats from Italy. All stool samples were subjected to flotation and Baermann's test. The cats were categorized in three groups based on the results of copromicroscopy, i.e., Group A (n. 50 cats with A. abstrusus infection regardless of positivity for other helminths), Group B (n. 25 cats negative for A. abstrusus but positive for at least one of any other helminth), Group C (n. 25 cats negative for any helminth). DNA was extracted from individual aliquots of feces, flotation supernatant, Baermann's sediment and the pharyngeal swab and then subjected to a PCR specific for A. abstrusus. At least one fecal aliquot or the pharyngeal swab scored positive by the A. abstrusus-specific PCR for 48/50 (96%) cats enrolled in Group A; in particular, 38/50 (76%), 35/50 (70%), 41/50 (82%) and 21/25 (84%) DNA extracts from feces, flotation supernatant, Baermann's sediment and pharyngeal swabs were positive by PCR. These results confirm that molecular tools are highly sensitive and specific and indicate that pharyngeal swabs are the most suitable sample for molecular analysis in clinical settings.

15.
Exp Parasitol ; 121(1): 92-5, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18950625

RESUMO

Cyathostome populations in horses on two farms located in central Italy with a history of fenbendazole (FBZ) resistance were investigated with the Faecal Egg Count Reduction Test to evaluate the susceptibility to oxibendazole and moxidectin. Faecal eggs were collected pre- and post-treatment on each farm and molecularly examined with a Reverse Line Blot (RLB) assay able to unequivocally detect and identify 13 cyathostome species. Resistance to FBZ was confirmed on both farms, while oxibendazole and moxidectin demonstrated 97% and 100% efficacy, respectively. Overall eight species of cyathostomes (Coronocyclus labiatus, Cylicocyclus ashworthi, Cylicocyclus nassatus, Cyathostomum catinatum, Cylicostephanus longibursatus, Cylicostephanus goldi, Cylicostephanus calicatus and Cylicocyclus insigne) were identified in pre-treatment samples. Coronocyclus labiatus and C. goldi were identified after treatment with FBZ while C. calicatus and C. labiatus were shown to be <100% susceptible to oxibendazole. These data confirm that resistance to benzimidazoles is established in cyathostome populations from horse farms in Italy and that they are susceptible to moxidectin. The oxibendazole has been successfully demonstrated for the first time as effective against Italian populations of cyathostomes resistant to other benzimidazoles. The RLB assay herein used showed to be useful to study the distribution of these parasitic populations at species level under field conditions and could represent a powerful tool in broader investigation of drug resistance in horse farms from several countries.


Assuntos
Antinematódeos/farmacologia , Benzimidazóis/farmacologia , Fenbendazol/farmacologia , Infecções Equinas por Strongyloidea/parasitologia , Strongyloidea/efeitos dos fármacos , Animais , Resistência a Medicamentos , Fezes/parasitologia , Cavalos , Macrolídeos/farmacologia , Hibridização de Ácido Nucleico/métodos , Sondas de Oligonucleotídeos , Contagem de Ovos de Parasitas/veterinária , Testes de Sensibilidade Parasitária/veterinária , Distribuição Aleatória , Especificidade da Espécie , Infecções Equinas por Strongyloidea/tratamento farmacológico , Strongyloidea/classificação , Strongyloidea/genética
16.
Exp Parasitol ; 122(4): 349-52, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19463815

RESUMO

Cryptosporidiosis is a worldwide-diffused protozoan disease causing important economic losses to animal husbandry and livestock production. Additionally, several species/genotypes of Cryptosporidium have a relevant zoonotic potential and ruminants may be important sources of infection for human beings. Nonetheless, in Europe, little is known of the presence of Cryptosporidium in sheep nor on the species/genotypes involved. To obtain information on the occurrence of cryptosporidiosis in lambs and the potential zoonotic role of the Cryptosporidium isolates, one hundred and forty-nine faecal samples individually collected from lambs in central Italy have been examined for the presence of Cryptosporidium. All faecal specimens were processed with a commercial ELISA kit immunoassay and all ELISA-positive samples were further analyzed genetically. Twenty-six ELISA-positive samples scored positive at the PCR and the sequences obtained displayed 100% identity with the zoonotic Cryptosporidum parvum. This work suggests for the first time that lambs in Italy may shed C. parvum, thus representing a potential public health hazard.


Assuntos
Criptosporidiose/veterinária , Cryptosporidium parvum/isolamento & purificação , Doenças dos Ovinos/parasitologia , Animais , Antígenos de Protozoários/análise , Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Cryptosporidium parvum/genética , Cryptosporidium parvum/imunologia , DNA de Protozoário/análise , Ensaio de Imunoadsorção Enzimática/veterinária , Fezes/parasitologia , Genótipo , Itália/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Ovinos , Doenças dos Ovinos/epidemiologia
17.
Vet Parasitol ; 160(1-2): 171-4, 2009 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-19042091

RESUMO

Five horse farms located in eastern Slovakia were investigated for the presence of benzimidazole-resistant strongyles by faecal egg count reduction test and egg hatch assay. Coprocultures were prepared for each farm from faecal samples taken pre- and post-treatment and harvested larvae were molecularly examined with a Reverse Line Blot assay. Faecal egg count reduction values ranged from 0 to 52.5% and all farms were positive for benzimidazole-resistant cyathostomins. Seven benzimidazole-resistant cyathostomin species were molecularly identified on farms before and also after treatment. These data demonstrate that resistance to benzimidazoles is well established in cyathostomin populations from horse farms in the Slovak Republic and that the molecular assay was able to determine the species-specific distribution of resistant cyathostomins under field conditions.


Assuntos
Anti-Helmínticos/uso terapêutico , Benzimidazóis/uso terapêutico , Doenças dos Cavalos/parasitologia , Infecções por Strongylida/veterinária , Estrongilídios/classificação , Técnicas do Sistema de Duplo-Híbrido/veterinária , Animais , Doenças dos Cavalos/diagnóstico , Doenças dos Cavalos/epidemiologia , Cavalos , Eslováquia/epidemiologia , Especificidade da Espécie , Infecções por Strongylida/tratamento farmacológico , Infecções por Strongylida/epidemiologia
18.
Parasitol Res ; 104(5): 979-84, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19057927

RESUMO

The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.


Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/genética , Mitocôndrias/enzimologia , Filogenia , Spiruroidea/classificação , Spiruroidea/genética , Substituição de Aminoácidos/genética , Animais , DNA Mitocondrial/química , DNA Mitocondrial/genética , Haplótipos , Doenças dos Cavalos/parasitologia , Cavalos , Itália , Dados de Sequência Molecular , Polimorfismo Genético , Análise de Sequência de DNA , Homologia de Sequência , Infecções por Spirurida/veterinária , Spiruroidea/isolamento & purificação
19.
Parasitol Res ; 105 Suppl 1: S55-62, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19575226

RESUMO

The objective of the present study was to evaluate the efficacy and safety of the antiparasitic spot-on formulation containing imidacloprid 10%/moxidectin 1% (Advocate, Bayer) in the treatment of natural feline infection with the lungworm Aelurostrongylus abstrusus (Nematoda, Strongylida). The efficacy of Advocate administered once was tested in comparison to a control oral formulation containing fenbendazole 18.75% (Panacur Intervet) administered over three consecutive days based on larvae per gramme of faeces (LPG), measured on days 28 +/- 2 following treatment and compared to counts on days -6 to -2. In total 24 cats treated either with Advocate (n = 12) or with Panacur (n = 12) were included. Mean LPG postbaseline (days 28 +/- 2) were low in both treatment groups, i.e., 0 LPG for Advocate and 1.3 LPG for Panacur. Reduction of post-baseline larval counts showed Advocate (100% reduction) to be superior in efficacy compared to the control product (99.29% reduction). No treated animals showed adverse events. This trial demonstrated that both Advocate spot-on formulation and Panacur oral paste are safe and effective in the treatment of aelurostrongylosis in cats. Future practical perspectives in feline medicine and the major advantages of the spot-on product compared to the oral paste are discussed.


Assuntos
Anti-Helmínticos/uso terapêutico , Doenças do Gato/tratamento farmacológico , Imidazóis/uso terapêutico , Metastrongyloidea/efeitos dos fármacos , Nitrocompostos/uso terapêutico , Infecções por Strongylida/veterinária , Animais , Anti-Helmínticos/administração & dosagem , Anti-Helmínticos/efeitos adversos , Doenças do Gato/parasitologia , Gatos , Método Duplo-Cego , Quimioterapia Combinada , Fezes/parasitologia , Fenbendazol/efeitos adversos , Fenbendazol/uso terapêutico , Imidazóis/administração & dosagem , Imidazóis/efeitos adversos , Larva/efeitos dos fármacos , Macrolídeos/administração & dosagem , Macrolídeos/efeitos adversos , Macrolídeos/uso terapêutico , Neonicotinoides , Nitrocompostos/administração & dosagem , Nitrocompostos/efeitos adversos , Infecções por Strongylida/tratamento farmacológico , Infecções por Strongylida/parasitologia , Resultado do Tratamento
20.
Parasitol Res ; 105 Suppl 1: S83-9, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19575229

RESUMO

The objective of the present study was to evaluate the efficacy and safety of the antiparasitic spot-on formulation containing emodepside 2.1%/praziquantel 8.6% (Profender, Bayer) in the treatment of natural feline infection with the lungworm Aelurostrongylus abstrusus (Nematoda, Strongylida). Efficacy of Profender given once at the licensed dose was tested in comparison to a control oral formulation containing fenbendazole 18.75% (Panacur, Intervet) given over three consecutive days at the licensed dose. Efficacy assessment was based on larvae per gramme of faeces (LPG) counts, measured on days 28 +/- 2 following treatment and compared to counts on days -6 to -2. In total 24 cats treated either with Profender (n = 12) or with Panacur (n = 12) were included in the assessment of efficacy and safety. Mean LPG post-baseline counts (days 28 +/- 2) were 1.3 LPG for both Profender and Panacur, demonstrating similar efficacy of 99.38% for Profender and 99.29% for the control product. No treated animals showed adverse events. This trial demonstrated that both Profender spot-on formulation and oral paste Panacur are safe and effective in the treatment of aelurotrongylosis in cats. Future practical perspectives in feline medicine and the major advantages of the spot-on product compared to the oral paste are discussed.


Assuntos
Anti-Helmínticos/uso terapêutico , Doenças do Gato/tratamento farmacológico , Depsipeptídeos/uso terapêutico , Praziquantel/uso terapêutico , Infecções por Strongylida/veterinária , Animais , Anti-Helmínticos/administração & dosagem , Anti-Helmínticos/efeitos adversos , Doenças do Gato/parasitologia , Gatos , Depsipeptídeos/administração & dosagem , Depsipeptídeos/efeitos adversos , Método Duplo-Cego , Combinação de Medicamentos , Fezes/parasitologia , Fenbendazol/administração & dosagem , Fenbendazol/efeitos adversos , Fenbendazol/uso terapêutico , Larva/efeitos dos fármacos , Metastrongyloidea/efeitos dos fármacos , Metastrongyloidea/isolamento & purificação , Praziquantel/administração & dosagem , Praziquantel/efeitos adversos , Infecções por Strongylida/tratamento farmacológico , Infecções por Strongylida/parasitologia , Resultado do Tratamento
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