Oxidative stress-A key determinant of complications and negative outcome in hepatitis E virus infected pregnancies: A comprehensive account involving cases from northeast India.

Regulated oxidative stress (OS) is important during pregnancy. Sporadic studies suggest the significance of deregulated OS in hepatitis E virus (HEV) infected pregnancy, but with limited reactive oxygen species (ROS) or antioxidant markers. The present novel study, therefore, aimed to evaluate the significance of ROS-antioxidant imbalance and resulting altered OS in HEV infected pregnancy complications like preterm delivery (PTD) and outcome. Difference in serum levels of ROS and antioxidant panel of markers were evaluated by ELISA for HEV immunoglobulin M RNA positive genotype 1 cases (including acute [acute viral hepatitis, AVH] and fulminant [fulminant hepatic failure, FHF] cases) and healthy term delivery subjects, and analyzed statistically. Direct ROS marker H2 O2 levels and indirect OS marker for DNA damage 8-hydroxy-2'-deoxyguanosine was significantly increased in HEV-cases compared to controls, and was associated and prognostic factor for PTD and fetal death in HEV cases. A comparatively lower total serum antioxidant capacity was observed in the FHF cases compared to the control subjects and the AVH cases. Glutathione (GSH) levels and superoxide dismutase (SOD) activity were significantly associated with PTD in the FHF sub-cohorts (p = 0.017) and AVH sub-cohorts (p < 0.001), respectively, and was associated with poor prognosis in HEV cases. The serum H2 O2 levels were found to be negatively correlated with SOD activity (p = 0.016) and GSH levels (p = 0.001) in the HEV-AVH cases; and positively correlated with the viral load in HEV cases (p = 0.023). The ROS-antioxidant imbalance resulting OS plays a detrimental associative role in HEV infected pregnancy complications like PTD and adverse pregnancy outcomes; and holds therapeutic significance.

A Phase 3, Randomized, Open-label, Noninferiority Trial Evaluating Anti-Rabies Monoclonal Antibody Cocktail (TwinrabTM) Against Human Rabies Immunoglobulin (HRIG).

BACKGROUND: Limited supply, cost and potential for severe adverse effects observed with the blood derived rabies immunoglobulin products has led to search for alternative therapies. This issue has been addressed by developing an anti-rabies monoclonal antibody cocktail. METHODS: This is a phase 3, randomized, open-label, noninferiority trial conducted in patients with World Health Organization (WHO) category III exposure with suspected rabid animal. Eligible patients were assigned to either the test arm, TwinrabTM (docaravimab and miromavimab) or the reference arm, human rabies immunoglobulin (HRIG; Imogam® Rabies-HT), in a ratio of 1:1. The primary endpoint was the comparison of responder rates between the 2 arms assessed as percentage of those with rabies virus neutralizing antibodies titers ≥0.5 IU/mL on day 14. RESULTS: A total of 308 patients were equally randomized into the 2 arms. In the per-protocol (PP) population, there were 90.21% responders in the TwinrabTM arm and 94.37% in the HRIG arm. The geometric mean of rapid fluorescent foci inhibition test titers in the PP on day 14 were 4.38 and 4.85 IU/mL, for the TwinrabTM and HRIG arms, respectively. There were no deaths or serious adverse events reported. CONCLUSIONS: This study confirmed that TwinrabTM is noninferior to HRIG in terms of providing an unbroken window of protection up to day 84. This trial in healthy adults with WHO category III exposure from suspected rabid animal also establishes the safety of TwinrabTM in patients with 1 WHO approved vaccine regimen (Essen). CLINICAL TRIALS REGISTRATION: CTRI/2017/07/009038.

Azide-Coordination in Homometallic Dinuclear Lanthanide(III) Complexes Containing Nonequivalent Lanthanide Metal Ions: Zero-Field SMM Behavior in the Dysprosium Analogue.

A series of homometallic dinuclear lanthanide complexes containing nonequivalent lanthanide metal centers [Ln2(LH2)(LH)(CH3OH)(N3)]·xMeOH·yH2O [1, Ln = DyIII, x = 0, y = 2; 2, Ln = TbIII, x = 1, y = 1] have been synthesized [LH4 = 6-((bis(2-hydroxyethyl)amino)-N'-(2-hydroxybenzylidene)picolinohydrazide] and characterized. The dinuclear assembly contains two different types of nine-coordinated lanthanide centers, because the nonequivalent binding of the azide co-ligand as well as the varying coordination of the deprotonated Schiff base ligand. Detailed magnetic studies have been performed on the complexes 1 and 2. Complex 1 and its diluted analogue (15%) are zero-field SMMs with effective energy barriers (Ueff) of magnetization reversal equal to 59(3) K and 66(3) K and relaxation times of τ0 = 10(4) × 10-6 s and 10(4) × 10-8 s, respectively. On the other hand, complex 2 shows a field-induced SMM behavior. Combined ab initio and density functional theory calculations were performed to explain the experimental findings and to unravel the nature of the magnetic anisotropy, exchange-coupled spectra, and magnetic exchange interactions between the two lanthanide centers.

Pentagonal Bipyramidal Ln(III) Complexes Containing an Axial Phosphine Oxide Ligand: Field-induced Single-ion Magnetism Behavior of the Dy(III) Analogues.

A series of neutral homologous complexes [(L)Ln(Cy3PO)Cl] {where Ln = Gd (1), Tb (2), Dy (3), and Er (5)} and [(L)Dy(Ph3PO)Cl] (4) [H2L = 2,6-diacetylpyridine bis-benzoylhydrazone] were isolated. In these complexes, the central lanthanide ion possesses a pentagonal bipyramidal geometry with an overall pseudo D5h symmetry. The coordination environment around the lanthanide ion comprises of three nitrogen and two oxygen donors in an equatorial plane. The axial positions are taken up by a phosphine oxide (O donor) and a chloride ion. Among these compounds, the Dy(III) (3 and 4) analogues were found to be field-induced single-ion magnets.

CAAC-Based Thiele and Schlenk Hydrocarbons.

Diradicals have been of tremendous interest for over a century ever since the first reports of p- and m-phenylene-bridged diphenylmethylradicals in 1904 by Thiele and 1915 by Schlenk. Reported here are the first examples of cyclic(alkyl)(amino)carbene (CAAC) analogues of Thiele's hydrocarbon, a Kekulé diradical, and Schlenk's hydrocarbon, a non-Kekulé diradical, without using CAAC as a precursor. The CAAC analogue of Thiele's hydrocarbon has a singlet ground state, whereas the CAAC analogue of Schlenk's hydrocarbon contains two unpaired electrons. The latter forms a dimer, by an intermolecular double head-to-tail dimerization. This straightforward synthetic methodology is modular and can be extended for the generation of redox-active organic compounds.

Influence of N-Substitution on the Formation and Oxidation of NHC-CAAC-Derived Triazaalkenes.

We have studied the effect of N-substitution on the course of the reaction of imidazolium triflate. The reaction of N-heterocyclic carbene with N-tBu-substituted pyrrolinium triflate afforded 2-(pyrrolidin-2-yl)-imidazolium triflate, 3R. Treatment of 3R with potassium bis(trimethylsilyl)amide (KHMDS) leads to either the dealkylation product 4 or the deprotonation product, triazaalkene 5, depending on the N-substitution at the imidazolium moiety. Density functional studies using the B3LYP/TZVP setup have been employed to explore various pathways for the dealkylation reaction and the calculated energies support the dealkylation by a large energy margin compared to the deprotonatation process. Theoretical calculations revealed that dealkylation reaction is thermodynamically more favorable than deprotonation. The triazaalkene 5 could be oxidized by AgOTf to the corresponding radical cation 6 and dication 7 in-situ. While 6 and 7 could not be isolated, the formation of the former is inferred by electron paramagnetic resonance spectroscopy and its abstraction of a H-atom to afford 3Me. Similarly, the formation of the dication 7 is inferred by its ready elimination of isobutylene affording 8.

"Abnormal" Addition of NHC to a Conjugate Acid of CAAC: Formation of N-Alkyl-Substituted CAAC.

The addition reactions of N-heterocyclic carbenes (NHCs) are mostly known to occur through the carbenic centre (C2), which leads to a "normal" adduct. Herein, we report the "abnormal" addition of NHCDip 1 (1,3-(2,6-iPr2 C6 H3 )-imidazole-2-ylidene) to a conjugate acid of cyclic (alkyl)(amino)carbene 2 (CAACiPr =1-iPr-3,3,5,5-Me4 -pyrrolinium triflate). Mechanistic study revealed that this reaction proceeded through the in situ formation of 1,3-(2,6-iPr2 C6 H3 )-imidazolium cation 4 and N-iPr-substituted CAAC 5 followed by the oxidative addition of compound 5 across the C4-H bond (alias backbone C-H) of compound 4. The in situ formation of compound 5 was also proven by the oxidative addition of it to the N-H group of iPrNH2 . DFT calculations also supported the mechanistic findings. A different methodology for the in situ generation of compound 5 by using TMPLi is also described.

Pentagonal-Bipyramid Ln(III) Complexes Exhibiting Single-Ion-Magnet Behavior: A Rational Synthetic Approach for a Rigid Equatorial Plane.

A pentadentate chelating ligand is employed for the facile synthesis of air-stable pentagonal-bipyramid Ln(III) complexes with a rigid equatorial plane. The Dy(III) analogue exhibits single-ion-magnet behavior with Ueff/ kB = 70 K under Hdc = 500 Oe.

Mononuclear Lanthanide Complexes: Energy-Barrier Enhancement by Ligand Substitution in Field-Induced DyIII SIMs.

The sequential reaction of 2-((6-(hydroxymethyl)pyridin-2-yl)-methyleneamino)phenol (LH2), LnCl3·6H2O, and 1,1,1-trifluoroacetylacetone (Htfa) in the presence of Et3N afforded [Ln(LH) (tfa)2] [Ln = Dy3+ (1), Ln = Tb3+ (2), and Ln = Gd3+ (3)], while under the same reaction conditions, but in the absence of the coligand, another series of mononuclear complexes, namely, [Ln(LH)2]·Cl·2MeOH] [Ln = Dy3+ (4) and Tb3+ (5)] are obtained. Single-crystal X-ray diffraction analysis revealed that the former set contains a mono-deprotonated [LH]- and two tfa ligands, while the latter set comprises of two mono-deprotonated [LH]- ligands that are nearly perpendicular to each other at an angle of 86.9°. Among these complexes, 2 exhibited a ligand-sensitized lanthanide-characteristic emission. Analyses of the alternating current susceptibility measurements reveal the presence of single-molecule magnet behavior for 1 and 4, in the presence of direct-current field, with effective energy barriers of 4.6 and 44.4 K, respectively. The enhancement of the effective energy barrier of the latter can be attributed to the presence of a large energy gap between the ground and first excited Kramers doublets, triggered by the change in coordination environments around the lanthanide centers.

Eight-coordinate mono- and dinuclear Dy(III) complexes containing a rigid equatorial plane and an anisobidentate carboxylate ligand in the axial position: synthesis, structure and magnetism.

A rigid pentadentate chelating ligand (H2L) has been utilized to synthesize a series of octacoordinate mononuclear complexes, [Dy(L)(Ph3PO)(OOCR)] (where R = C6H5 (1), C(CH3)3 (2), CF3 (3)) and a dinuclear complex, [Dy2(L)2(Ph3PO)2{(OOC)2C6H4}] (4) based on the highly anisotropic Dy(III) ion. All the complexes were structurally characterized by single-crystal X-ray diffraction studies. The complexes were formed by the coordination action of the dianionic pentadentate ligand [L]2-, one phosphine oxide, and carboxylate ligands. DC and AC magnetic measurements were performed on 1-4. Complexes 1-4 show SMM behaviour, under zero DC field for 1 and 4, and under 500 Oe and 1000 Oe DC fields for 2 and 3 respectively, with thermally activated, Raman, and Raman and quantum tunnelling dominant relaxation mechanisms for 1 and 2, 3 and 4, respectively.

In situ hydrolysis of a carbophosphazene ligand leads to one-dimensional lanthanide coordination polymers. Synthesis, structure and dynamic magnetic studies.

An in situ hydrolysis of the P-Cl bonds of the carbophosphazene [{NC(NMe2)}2{NPCl2}] (LPCl2) in the presence of hydrated lanthanide(III) nitrates in a dichloromethane and methanol (2 : 1) solvent mixture afforded a series of novel 1D coordination polymers: [{Ln(LHPO2)3(NO3)2(CH3OH)(H2O)} (Cl)]n {where Ln(III) = Gd (1), Tb (2), Dy (3), or Er (4) and LHPO2 is the hydrolyzed carbophosphazene (LPCl2) ligand}. X-ray crystallographic analysis revealed that complexes 1-4 are isostructural and crystallized in the monoclinic crystal system having P21/c space group. The coordination polymers are formed because of the involvement of the geminal P(O)(OH) moieties of the carbophosphazene ligand. Each lanthanide(III) ion is 9-coordinate (9O) in a distorted muffin geometry. Magnetic measurements revealed that both DyIII and ErIII analogues exhibit field-induced single-molecule magnet (SMM) behavior at 0.8 kOe and 2.2 k Oe, respectively. At such dc fields, the dynamic magnetic susceptibility displays complex behavior with a triple magnetic relaxation contribution for 3, while two contributions were identified for 4. The observed static and dynamic magnetic behavior for complexes 1-4 were further rationalized with the aid of BS-DFT and CASSCF/SO-RASSI/SINGLE_ANISO calculations.

Sustainable hydrogen generation and storage - a review.

In 21st century, the energy demand has grown incredibly due to globalization, human population explosion and growing megacities. This energy demand is being mostly fulfilled by fossil-based sources, which are non-renewable and a major cause of global warming. Energy from these fossil-based sources is cheaper, however challenges exist in terms of climate change. This makes renewable energy sources more promising and viable for the future. Hydrogen is a promising renewable energy carrier for fulfilling the increasing energy demand due to its high energy density, non-toxic and environment friendly characteristics. It is a non-toxic energy carrier as combustion of hydrogen produces water as the byproduct whereas other conventional fuels produce harmful gases and carcinogens. Because of its lighter weight, hydrogen leaks are also easily dispersed in the atmosphere. Hydrogen is one of the most abundant elements on Earth, yet it is not readily available in nature like other fossil fuels. Hence, it is a secondary energy source and hydrogen needs to be produced from water or biomass-based feedstock for it to be considered renewable and sustainable. This paper reviews the renewable hydrogen generation pathways such as water splitting, thermochemical conversion of biomass and biological conversion technologies. Purification and storage technologies of hydrogen is also discussed. The paper also discusses the hydrogen economy and future prospects from an Indian context. Hydrogen purification is necessary because of high purity requirements in particular applications like space, fuel cells etc. Various applications of hydrogen are also addressed and a cost comparison of various hydrogen generation technologies is also analyzed. In conclusion, this study can assist researchers in getting a better grasp of various renewable hydrogen generation pathways, it's purification and storage technologies along with applications of hydrogen in understanding the hydrogen economy and its future prospect.

Synthesis, structures and magnetic studies of hexanuclear lanthanide complexes: SMM behavior of the DyIII analogue and MCE properties of the GdIII analogue.

The synthesis, structure and magnetic properties of homometallic hexanuclear lanthanide complexes [Ln6(HL)4(tfa)4(S)2]·2NO3·x H2O·yMeOH (1, Ln = Gd, S = MeOH, x = 0, y = 0; 2, Ln = Tb, S = H2O, x = 2, y = 2; 3, Ln = Dy, S = MeOH, x = 0, y = 2; 4, Ln = Er, S = MeOH, x = 0, y = 2). [(H4L) = 6-((bis(2-hydroxyethyl)amino)-N'-(2-hydroxybenzylidene)picolinohydrazide) (tfa = trifloroacetylacetone)] are reported. These hexanuclear assemblies are made up of two trinuclear triangular sub-units linked through the oxygen atoms of two phenoxide bridging groups in a corner sharing arrangement. Magnetic studies reveal that 1 displays a magnetocaloric effect with a maximum value of -ΔSm = 21.03 J kg-1 K-1 at T = 3 K and under an applied field change ΔB = 5 T. Complex 3 shows slow relaxation of magnetization even under zero applied field although a clear maximum in the ac susceptibility plots cannot be seen. However, under an optimal applied field of 0.2 T, clear maxima are observed in the out-of-phase (χ''M) component of the ac susceptibility in the temperature range 3.5 K (2 kHz) to 10.5 K (10 kHz). The temperature dependence of the relaxation times could be fitted to the sum of Orbach, Raman and QTM relaxation processes affording the following parameters: τo = 3.4(9) × 10-8 s, Ueff = 94(2) K, BRaman = 16.43(1) K-n s-1, n = 3.2(3) and τQTM = 0.0044(3) s. 4, under an applied magnetic field of 0.2 T, shows slow relaxation of magnetization through a thermally activated Orbach process with Ueff = 18.2(9) K and τo = 3.5(3) × 10-8 s.

Self-Assembly Driven Formation of Functional Ultralong "Artificial Fibers" to Mitigate the Neuronal Damage Associated with Alzheimer's Disease.

Fibrillation of amyloid beta (Aß) is the key event in the amyloid neurotoxicity process that induces a chain of toxic events including oxidative stress, caspase activation, poly(ADP-ribose) polymerase cleavage, and mitochondrial dysfunction resulting in neuronal loss and memory decline manifesting as clinical dementia in humans. Herein, we report the development of a novel, biologically active supramolecular probe, INHQ, and achieve functional nanoarchitectures via a self-assembly process such that ultralong fibers are achieved spontaneously. With specifically decorated functional groups on INHQ such as imidazole, hydroxyquinoline, hydrophobic chain, and hydroxyquinoline molecules, these ultralong fibers coassembled efficiently with toxic Aß oligomers and mitigated the amyloid-induced neurotoxicity by blocking the aforementioned biochemical events leading to neuronal damage in mice. These functional ultralong "Artificial Fibers" morphologically resemble the amyloid fibers and provide a higher surface area of interaction that improves its clearance ability against the Aß aggregates. The efficacy of this novel INHQ molecule was ascertained by its high ability to interact with Aß. Moreover, this injectable, ultralong INHQ functional "artificial fiber" translocates through the blood-brain barrier and successfully attenuates the amyloid-triggered neuronal damage and pyknosis in the cerebral cortex of wild-type mouse. Utilizing various spectroscopic techniques, morphology analysis, and in vitro, in silico, and in vivo studies, these ultralong INHQ fibers are proven to hold great promise for treating neurological disorders at all stages with a potential to replace the existing medications, reduce complications in the brain, and eradicate the amyloid-triggered neurotoxicity implicated in numerous disorders in human through a rare synergistic mechanism.

Slow magnetic relaxation in a homoaxially phosphine oxide coordinated pentagonal bipyramidal Dy(III) complex.

We report the synthesis of [(L)DyIII(Cy3PO)2]·[BPh4] (1-Dy) (where H2L = 2,6-diacetylpyridine bis-benzoylhydrazone and Cy = cyclohexyl) which crystallized in the triclinic, P1̄ space group. The local geometry around Dy(III) in 1-Dy was found to be pentagonal bipyramidal (pseudo-D5h). The AC magnetic susceptibility measurements performed on 1-Dy and on its diluted 1-Y(Dy) samples showed a typical single-molecule magnet signature revealed by the appearance of AC-frequency dependent out-of-phase susceptibility signals in the absence of a static magnetic field. The out-of-phase AC susceptibility signals were well resolved on the application of a small magnetic field (HDC = 500 Oe) and yielded an energy barrier for magnetization flipping of Ueff/kB = 50 K for the diluted derivative. The magnetic studies on 1-Dy and 1-Y(Dy) and data analysis further confirm that Raman and QTM under-barrier magnetic relaxations play a crucial role in lowering Ueff despite the almost axial nature of the Dy(III) ion in 1-Dy. We have rationalized these observations through detailed ab initio calculations performed on the X-ray crystal structure of 1-Dy.

Energy-efficient biochar production for thermal backfill applications.

The function of engineered thermal backfills surrounding underground pipelines of the crude oil industry is to prohibit heat migration for the design period of 25 to 50 years. Biochar is suitable for reconstituting standard thermal backfill material since it is biochemically inert and has a low heat conductivity. However, the preparation of biochar from biomass involves an energy-intensive pyrolysis process. This study aims to make biochar production energy-efficient via optimizing the pyrolysis temperatures, specifically for thermal backfill applications. Ten distinct biochars were prepared by pyrolyzing two waste biomass, i.e., water hyacinth (WH) and sugarcane bagasse (SB), at temperatures ranging from 300 to 700 °C. The biochars were assessed based on their thermal conductivity, energy consumption, yield, and stability in soil for the design period. The thermal conductivity of produced biochars varied in a narrow range of 0.10 to 0.13 W m-1 K-1 with different pyrolysis temperatures, which is possibly due to marginal differences in their microstructure, mineralogy, and physicochemical properties. The findings revealed that the biochar produced at lowest pyrolysis temperature (300 °C) consumed least energy and produced maximum yield. However, it was not suitable for thermal backfill applications due to its inadequate carbon stability in soil. Therefore, the current study recommends a pyrolysis temperature of 400 °C for thermal backfill applications. The recommended pyrolysis temperature was found to be at least 60% energy efficient in comparison to pyrolysis at 700 °C for both the feedstocks. This study provides crucial insight into the role of pyrolysis temperature for tailoring biochar production for intended applications.

Exploring the efficiency and pollutant emission of a dual fuel CI engine using biodiesel and producer gas: An optimization approach using response surface methodology.

The present study focuses on optimizing the engine operating parameters of a dual-fuel (DF) engine. Producer gas (PG) and Honge oil methyl ester (HOME) are used as primary fuel and pilot fuel respectively for the operation. An experimental design matrix of 20 different combinations was considered using Design of Experiments (DoE), based on the central composite design (CCD) of response surface methodology (RSM). The effects of these combinations were experimentally investigated to calculate the performance and emission characteristics of the engine. The objective of the work is to maximize the Brake thermal efficiency (BTE) and minimize the exhaust gas temperature (EGT), nitrogen oxide (NOx), hydrocarbon (HC), and carbon monoxide (CO) emissions. The RSM model is developed using the experimental data and further, the operating parameters were optimized using the desirability approach. The optimized combination of operating parameters was obtained at 61.10% engine load, compression ratio (CR) of 18, and injection timing (IT) of 23.30° before top dead center (BTDC). The optimum responses corresponding to these operating conditions were found as 14.23%, 354.29 °C, 52.18 ppm, 39.53 ppm, and 0.51% for BTE, EGT, NOx, HC, and CO respectively with an overall desirability of 0.962. The optimized responses were validated experimentally at optimum input conditions and found to be within acceptable error levels. Further, an economic analysis of the optimized DF system is also carried out.

Green synthesis of gold nanoparticles using an antiepileptic plant extract: in vitro biological and photo-catalytic activities.

Gold nanoparticles are one of the widely used metallic nanoparticle having unique surface plasmon characteristic, offers major utility in biomedical and therapeutic fields. However, chemically synthesized nanoparticle creates toxicity in the living organisms and contradicts the eco-friendly and cost-effective nature. So, developing greener synthetic route for synthesis of gold nanoparticle using natural materials is an enthralling field of research for its effectiveness in synthesizing eco-friendly, non-toxic materials. Moreover, biological components attached as stabilizing agent can exert its own effect along with the advantages of nanoparticle conjugation. In this work, we used for the first time methanolic leaf extract of Moringa oleifera as this fraction of M. oleifera exerts a neuroactive modulation against seizure as evidenced by earlier literature. The green gold nanoparticles synthesized were characterized by different characterization tools, dynamic light scattering and transmission electron microscopy techniques etc. Prepared nanoparticles were biologically (antioxidant, antimicrobial and blood cytotoxicity) characterized to screen their further utility in therapeutic strategies. Characteristics and activities of green gold nanoparticles were compared with conventional citrate stabilized gold nanoparticles. It was observed that green gold nanoparticles prepared using M. oleifera show less cytotoxicity and helps in regeneration of neuronal cells in animal model study. It establishes the fact that conjugation of different plant extract fraction for stabilization of gold nanoparticle may be responsible factor for enhancement of bioactive nature of green gold nanoparticle. In addition, the green gold nanoparticle show efficient photo-catalytic efficiency. Development of such bioactive gold nanoparticles will lead to functional materials for biomedical and therapeutic applications.

ZRC3308 Monoclonal Antibody Cocktail Shows Protective Efficacy in Syrian Hamsters against SARS-CoV-2 Infection.

We have developed a monoclonal antibody (mAb) cocktail (ZRC-3308) comprising of ZRC3308-A7 and ZRC3308-B10 in the ratio 1:1 for COVID-19 treatment. The mAbs were designed to have reduced immune effector functions and increased circulation half-life. mAbs showed good binding affinities to non-competing epitopes on RBD of SARS-CoV-2 spike protein and were found neutralizing SARS-CoV-2 variants B.1, B.1.1.7, B.1.351, B.1.617.2, and B.1.617.2 AY.1 in vitro. The mAb cocktail demonstrated effective prophylactic and therapeutic activity against SARS-CoV-2 infection in Syrian hamsters. The antibody cocktail appears to be a promising candidate for prophylactic use and for therapy in early COVID-19 cases that have not progressed to severe disease.