Response of rumen fermentation and microbiota to dietary supplementation of sodium selenite and bio-nanostructured selenium in lactating Barki sheep.

Dietary selenium (Se) sources affects the structure of the rumen microbial community and rumen fermentation. This study evaluated the effects of sodium selenite (SS) and bio-nanostructured selenium (SeNSM) on rumen fermentation and structure of rumen microbial community of lactating Barki ewes. Twenty one lactating Barki ewes were assigned into three groups based on their body weight and milk yield. The experiment lasted for 50 days, whenever, the control group was fed basal diet; group SS received basal diets plus sodium selenite as inorganic source of Se; and group SeNSM received basal diet plus organic selenium bio-nanostructured. Ruminal pH and volatile Fatty Acids (VFA) was lower (P < 0.05) in SeNSM group compared to control. Principle Coordinate Analysis separated the microbial communities into three clusters based on feeding treatment. The bacterial community was dominated by phylum Bacteroidetes and Firmicutes that were affected (P < 0.05) by Se sources. Specifically Bacteriodetes was higher (P < 0.05) in SS and SeNSM groups; and Firmicutes was higher (P < 0.05) in the control group. Moreover, the predominant bacterial genera were Prevotella, Rikenellaceae RC9 gut group, Unclassified_Bacteroidales, which were higher (P < 0.05) in SeNSM group. The methanogenic community belonged to phylum Euryarchaeota and was significantly decreased (P < 0.05) by Se supplementation. Principal component analysis based on rumen fermentation parameters, and relative abundances of bacteria and methanogens revealed three distinct clusters. These findings suggest that Se supplementation affected the relative abundances of dominant bacterial groups, declined rumen methanogens and SeNSM supplementation showed some positive impacts on some fibrolytic bacteria.

Comparative digestibility and rumen fermentation of camels and sheep fed different forage sources.

The present study evaluated the effects of forage sources on dry matter (DM) intake, digestibility, and fermentation parameters in camels vs. sheep. The study was arranged as a 2 × 3 factorial experiment in a completely randomized design by using two animal species (three ruminally cannulated female camels and three male sheep) and three forage sources. The forages were (1) alfalfa hay; 164 and 479 g/kg DM for crude protein (CP) and neutral detergent fiber (NDF), respectively, (2) berseem hay; 121 and 513 g/kg DM for CP and NDF, respectively, and (3) wheat straw (27.5 and 723 g/kg DM for CP and NDF, respectively). Higher DM intake [g/kg body weight (BW)] was noted in sheep compared to camel when alfalfa hay (p < 0.05) and berseem hay (p < 0.05) were fed but was similar between both species when they were fed wheat straw. Forage type rather than animal species had more effect on metabolic intake. Lower digestibility was noted in sheep with wheat straw (p < 0.05). Similar in situ degradability values for crude protein and DM were noted between camels and sheep. In situ degradability of NDF was higher (p < 0.05) in camel than sheep. Greater ruminal pH (p < 0.05) was noted in sheep vs. camels when berseem hay and wheat straw were fed. Lower ruminal passage rate (p < 0.05) was noted in camels and higher total mean retention time. It was concluded that sheep and camels had similar digestion capacities when fed berseem hay and alfalfa hay, however, camels are more efficient than sheep when fed wheat straw.

Growth performance, nutrient utilization, rumen fermentation, blood biochemistry, and carcass traits of lambs fed Atriplex nummularia L. hay-based diet supplemented with yeast or bacterial direct- fed microbial.

Direct fed microbial may enhance the utilization of halophyte forages leading to improved animal growth and productivity. This study was conducted to evaluate Atriplex hay-based diet supplemented with yeast (Saccharomyces cerevisiae; SC) or bacteria (Bacillus subtilis and Lactobacillus casei; BAC) on lamb growth performance, digestibility, rumen fermentation, and carcass characteristics. Fifteen Barki lambs (90 ± 7 days of age and 18.6 ± 0.41 kg SE body weight; BW) were randomly assigned to three treatments for 120 days as follows: Control (basal diet without supplementation), SC and BAC diets, the basal diet supplemented with SC or BAC at 2 g/head/day, respectively. All lambs had similar dry matter (DM) intake, while lambs fed SC or BAC dies had higher (P < 0.05) total gain and average daily gain than those fed the control diet. Supplementation of SC or BC increased (P < 0.05) the digestibility of DM, organic matter, and acid detergent fiber, tended to decrease (P < 0.05) the urine N excretion and enhanced the N balance compared to the control. Ruminal pH, acetate, total volatile fatty acids concentrations, and bacterial protein were increased (P < 0.05), while creatinine and urea concentrations were decreased (P < 0.05) by both additives. Compared to other diets, the BAC diet reduced (P < 0.05) triglycerides, total lipids, kidney fat, and eye muscle fat. In conclusion, both additives resulted in similar positive growth performance and feed utilization, while only the BAC additive had a beneficial advantage in reducing the fat content of the carcass.

Nutritional impact of nano zeolite, probiotic, and fatty acids as feed additives on health status of Nile tilapia (Oreochromis niloticus).

For human consumption, fish is a good and affordable source of several crucial elements. Growing aquaculture management and output is always necessary. Therefore, this study was designed to evaluate the effect of probiotics, nano zeolite, and/or medium chain fatty acids (MCFA) on fish health and the chemical composition of the fish body. The experimental fish were distributed into eight groups. T1: Control group fed the basal diet without feed additives, T2: Nano zeolite at a rate of 2 mg/kg diet, T3: Pedococcus at a rate of 2 gm kg diet, T4: Medium chain fatty acids used according to produced company recommendation at a rate of 3.5 gm/kg diet, T5: Nano zeolite (2 mg/kg diet) + Pedococcus (2 mg/kg diet), T6: Nano zeolite (2 mg/kg diet) + Medium chain fatty acids (3.5 gm/kg diet), T7: Pedococcus (2 mg/kg diet) + Medium chain fatty acids (3.5 gm/kg diet), and T8: Nano zeolite (2 mg/kg diet) + Pedococcus (2 mg/kg diet) + Medium chain fatty acids (3.5 gm/kg diet). The obtained results showed an insignificant difference in the body composition of Nile tilapia fish fed feed additives alone or in combination. Moreover, the feed additives did not affect the health status of fish, as indicated by normal liver and kidney functions.