RESUMO
This study aimed to to evaluate the stability of commonly used endogenous control genes for messenger RNA (mRNA) (N = 16) and miRNAs (N = 3) expression studies in prostate cell lines following irradiation. The stability of endogenous control genes expression in irradiated (6 Gy) versus unirradiated controls was quantified using NormFinder and coefficient of variation analyses. HPRT1 and 18S were identified as most and least stable endogenous controls, respectively, for mRNA expression studies in irradiated prostate cells. SNORD48 and miR16 miRNA endogenous controls tested were associated with low coefficient of variations following irradiation (6 Gy). This study highlights that commonly used endogenous controls can be responsive to radiation and validation is required prior to gene/miRNAs expression studies.
Assuntos
Perfilação da Expressão Gênica , Proteínas de Neoplasias/biossíntese , Neoplasias da Próstata/genética , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica/efeitos da radiação , Humanos , Masculino , MicroRNAs/biossíntese , MicroRNAs/efeitos da radiação , Proteínas de Neoplasias/efeitos da radiação , Neoplasias da Próstata/patologia , Neoplasias da Próstata/radioterapia , RNA Mensageiro/biossíntese , RadiaçãoRESUMO
This article describes the development of a computerized pharmacy order entry system using IBM's Patient Care System software at a large teaching hospital. The pharmacy component is part of a hospital-wide order entry system that includes other departments such as dietary, radiology, central supply, and laboratories. Pharmacy orders are entered into the computer system at the patient care unit by secretaries or nurses and subsequently checked and supplemented by pharmacists in satellite pharmacies. The system produces a medication administration record for use by nursing that becomes a permanent part of the patient medical record. Pharmacy cassette fill lists, i.v. labels, and charge summaries are produced. Billing is automatically accomplished upon the completion of an order. Initial hardware problems have been resolved and the system has been fully operational for 2 years. Ongoing development seeks to enhance the system's range of functions.