Effects of preservation methodology on stable isotope compositions of sea stars.

RATIONALE: Stable isotope analysis is used to investigate the trophic ecology of organisms and, in order to use samples from archived collections, it is important to know whether preservation methods alter the results. This study investigates the long-term effects of four preservation methods on isotopic compositions and isotopic niche parameters of sea stars. METHODS: We assessed the effects of preservation method (freezing, drying, formaldehyde, ethanol) and duration (0, 1, 3, 6, 9, 12, 24 months) on the stable isotope ratios of carbon, nitrogen and sulfur of sea star tissues. Isotopic ratios were measured using continuous-flow elemental analysis and isotope ratio mass spectrometry. We also monitored the evolution of commonly used ecological metrics (isotopic niche parameters) throughout the experiment. RESULTS: Clear changes in δ13 C values were observed for samples stored in formaldehyde and ethanol. None of the preservation methods had significant or consistent effects on δ15 N values. Formaldehyde preservation induced a decrease in δ34 S values. All these changes could be mitigated using correction factors. Isotopic niche parameters slightly changed over time when computed with δ13 C and δ15 N values, but inconsistent variations occurred when computed with δ13 C and δ34 S values. CONCLUSIONS: Overall, these results show that preservation may affect the stable isotope ratios of sea stars. Correction factors can be used to mitigate the effects of the preservation method on stable isotope ratios. Isotopic niche parameters are overall unchanged. Consequently, in most cases, museum samples are suitable for calculation of isotopic niche parameters.

Inter-individual differences in ontogenetic trophic shifts among three marine predators.

Ontogenetic niche shifts are widespread. However, individual differences in size at birth, morphology, sex, and personalities can cause variability in behavior. As such, inherent inter-individual differences within populations may lead to context-dependent changes in behavior with animal body size, which is of concern for understanding population dynamics and optimizing ecological monitoring. Using stable carbon and nitrogen isotope values from concurrently sampled tissues, we quantified the direction and magnitude of intraspecific variation in trophic shifts among three shark species, and how these changed with body size: spurdogs (Squalus spp.) in deep-sea habitats off La Réunion, bull sharks (Carcharhinus leucas) in estuarine habitats of the Florida Everglades, and blacktip reef sharks (Carcharhinus melanopterus) in coral reef ecosystems of Moorea, French Polynesia. Intraspecific variation in trophic shifts was limited among spurdogs, and decreased with body size, while bull sharks exhibited greater individual differences in trophic shifts, but also decreased in variability through ontogeny. In contrast, blacktip reef sharks exhibited increased intraspecific variation in trophic interactions with body size. Variability in trophic interactions and ontogenetic shifts are known to be associated with changes in energetic requirements, but can vary with ecological context. Our results suggest that environmental stability may affect variability within populations, and ecosystems with greater spatial and/or temporal variability in environmental conditions, and those with more diverse food webs may facilitate greater individual differences in trophic interactions, and thus ontogenetic trophic shifts. In light of concerns over environmental disturbance, elucidating the contexts that promote or dampen phenotypic variability is invaluable for predicting population- and community-level responses to environmental changes.

Effect of body length, trophic position and habitat use on mercury concentrations of sharks from contrasted ecosystems in the southwestern Indian Ocean.

The non-essential metal mercury (Hg) can have deleterious effects on health of organisms, and tends to bioaccumulate with age in long-lived organisms and to biomagnify along food webs. Because elasmobranchs are fished for human consumption and their Hg levels are frequently above the maximum Hg concentration recommended for fish consumption, understanding the drivers of Hg concentration is of considerable interest. Total Hg concentrations were analysed in muscle tissues of 14 shark and 2 batoid species (n = 339 individuals) sampled across multiple habitats (coastal, open ocean and bathyal) in the southwestern Indian Ocean. Stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) were analysed to assess whether relative trophic position and foraging habitats affected Hg concentrations. Hg concentrations increased with δ15N and body length, highlighting the mechanisms of bioaccumulation and biomagnification in relation with the trophic position and size of the individuals. Habitats where elasmobranchs were collected also affected their Hg concentrations. Bathyal sharks had high Hg concentrations that were almost similar to those of oceanic species, despite their lower relative trophic position. Higher bioavailability of Hg due to its enhanced methylation in deeper waters was considered as the most likely explanation for this result. These results highlight that multiple factors contribute to mercury accumulation in elasmobranchs.

Trophic markers and biometric measurements in Southern Ocean sea stars (1985-2017).

Sea stars (Echinodermata: Asteroidea) are a key component of Southern Ocean benthos, with 16% of the known sea star species living there. In temperate marine environments, sea stars commonly play an important role in food webs, acting as keystone species. However, trophic ecology and functional role of Southern Ocean sea stars are still poorly known, notably due to the scarcity of large-scale studies. Here, we report 24,332 trophic marker (stable isotopes and elemental contents of C, N, and S of tegument and/or tube feet) and biometric (arm length, disk radius, arm to disk ratio) measurements in 2,456 specimens of sea stars. Samples were collected between 12 January 1985 and 8 October 2017 in numerous locations along the Antarctic littoral and subantarctic islands. The spatial scope of the data set covers a significant portion of the Southern Ocean (47.717° S to 86.273° S; 127.767° W to 162.201° E; depth, 6-5,338 m). The data set contains 133 distinct taxa, including 72 currently accepted species spanning 51 genera, 20 families, and multiple feeding guilds/functional groups (suspension feeders, sediment feeders, omnivores, predators of mobile or sessile prey). For 505 specimens, mitochondrial CO1 genes were sequenced to confirm and/or refine taxonomic identifications, and those sequences are already publicly available through the Barcode of Life Data System. This number will grow in the future, as molecular analyses are still in progress. Overall, thanks to its large taxonomic, spatial, and temporal extent, as well as its integrative nature (combining genetic, morphological, and ecological data), this data set can be of wide interest to Southern Ocean ecologists, invertebrate zoologists, benthic ecologists, and environmental managers dealing with associated areas. Please cite this data paper in research products derived from the data set, which is freely available without copyright restrictions.