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1.
Environ Microbiol ; 20(10): 3529-3542, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30051625

RESUMO

Airborne bacteria are abundant and can vary with land use. Urban expansion is increasing rapidly at a global scale, altering natural sources of airborne bacterial biodiversity, as soils and native plants are replaced by pavement and managed yards. Urbanization homogenizes the biodiversity of larger organisms, but its effects are understudied with respect to microbes. This study uses categorical and gradient approaches to examine airborne bacterial communities in southwest Michigan (USA). Airborne communities carried a gut-microbial signature and were equally homogenous above urban and rural sites, despite greater homogeneity of soil communities at urban sites. Ruminococcaceae were abundant, the source of which is likely wildlife. Beyond the gut-microbial signature, there were underlying effects of land use, which were evident in the shared airborne taxa across urban and rural sites. Bacillales, Burkholderiales, Alteromonadales and Pseudomonadales were shared more across urban sites, while Xanthomonadales, which contains crop-plant pathogens, were shared across rural agricultural sites. These results suggest that taxa which may distribute globally, coupled with localized sources, contribute to urban communities, while regional rural activities drive rural composition. We determined that soils were unlikely to contribute to broad distribution of some plant-associated taxa, but may be a source for distribution of others.


Assuntos
Microbiologia do Ar , Bactérias , Biodiversidade , Bactérias/genética , Michigan , Plantas , Solo
2.
Rev Sci Instrum ; 95(2)2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-38354367

RESUMO

To completely characterize the evolving state of a plasma, diagnostic tools that enable measurements of the time-resolved behavior are required. In this study, a gridded ion source with superimposed oscillations was utilized to verify the functionality of a high-speed retarding potential analyzer (HSRPA), at frequencies equivalent to the low frequency oscillations occurring in Hall effect thrusters (HETs). The verification of this device provides an effective alternative to existing diagnostics for measuring time-resolved ion energies. Retarding potential analyzers (RPAs) have established themselves as a fundamental diagnostic in the field of electric propulsion (EP), enabling the measurement of ion energy distributions within the plumes of EP thrusters. The work presented here has demonstrated the capability of a standard RPA in conjunction with high-speed circuitry and data fusion techniques to produce time-resolved ion energy distribution functions (IEDFs) at higher frequencies and beam potentials than have previously been investigated. Tested frequencies ranged between 20 and 80 kHz with 10 V peak-to peak oscillations at a mean beam potential of 570 V. In addition, measurements were conducted with several waveforms, functioning as the superimposed oscillation, including a sine wave, triangle wave, and noisy sine wave. Data from the HSRPA were successfully reconstructed into time series utilizing two data fusion techniques: the empirical transfer function method and shadow manifold interpolation. Time-resolved IEDFs were produced at all frequency set points and waveforms. This investigation has demonstrated the HSRPA effectiveness at producing time-resolved measurements under conditions similar to those occurring in HETs.

3.
Work ; 75(4): 1199-1213, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36744355

RESUMO

BACKGROUND: Many organizations are undertaking efforts to reduce the stress of (oftentimes overworked) employees. Information Technology (IT) (e.g., smartphones) has the potential to be a key instrument for reducing stress. One design-relevant factor considered to reduce stress is the concept of autonomy. Unfortunately, little research exists using autonomy as a characteristic of technology design. OBJECTIVE: Against this background, this study aimed to investigate specific autonomy-related design options with the potential to prevent stress. METHODS: In a factorial survey, this experimental study tested three design options in an overwork scenario: 1) autonomy (no intervention by design), 2) nudge ("nudging" by design), and 3) enforcement (hard stop by design). 51 participants (mean age 38 years, 50% women, mean work experience 18 years) from the Netherlands, United Kingdom, United States of America, and Germany participated in the experiment for 330 seconds on average. To test our hypothesis, we used a two-step approach. First, a multiple linear regression was applied. Second, we carried out a one-way ANCOVA comparing the effects of our design options. RESULTS: Our results indicate that autonomy can be manipulated through technology design and is negatively correlated with stress. Additionally, the design options autonomy and nudge were associated with lower levels of perceived stress than was enforcement. CONCLUSION: The study proposes a careful use of IT and policies that limit the perceived autonomy of employees. Overall, this study offers a set of design recommendations arguing that organizations should implement technology that helps employees prevent overwork and maintain their autonomy.


Assuntos
Estresse Ocupacional , Autonomia Pessoal , Adulto , Feminino , Humanos , Masculino , Alemanha , Países Baixos , Reino Unido , Estados Unidos , Estresse Ocupacional/prevenção & controle
4.
Rev Sci Instrum ; 92(7): 073306, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34340440

RESUMO

A retarding potential energy analyzer was used to obtain temporally resolved ion energy distribution functions (IEDFs) of a flowing laboratory plasma. The plasma of time varying ion energy was generated at 1 and 20 kHz using a commercial gridded ion source and modulated using a wideband power amplifier. Three plasma energy modulation setpoints were tested, and their IEDFs were reconstructed. This method leverages high-speed, low-noise instrumentation to obtain fast collector current measurements at discrete retarding bias levels, recombining them in the time domain using two data fusion techniques. The first method is an empirical transfer function, which determines the linear ratio of complex coefficients in Fourier space. The second method, shadow manifold interpolation, reconstructs the IEDFs point-by-point by comparing input and output datasets in a multi-dimensional phase space. Reconstructed IEDFs from the two methods are presented and compared. The two analysis methods show very good agreement.

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