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The pathway mediated by jasmonic acid (JA), biosynthesized via 13-lipoxygenases (LOX), plays a central role in both plant development and defense. In rice, there are at least fourteen 13-LOXs. Yet, only two 13-LOXs have been known to be involved in the biosynthesis of JA and plant defenses in rice. Here we cloned a chloroplast-localized 13-LOX gene from rice, OsRCI-1, whose transcripts were upregulated following infestation by brown planthopper (BPH, Nilaparvata lugens), one of the most important pests in rice. Overexpression of OsRCI-1 (oeRCI lines) increased levels of BPH-induced JA, jasmonate-isoleucine, trypsin protease inhibitors and three volatile compounds, 2-heptanone, 2-heptanol and α-thujene. BPHs showed a decreased colonization, fecundity and mass, and developed slowly on oeRCI plants compared with wild-type (WT) plants. Moreover, BPH-infested oeRCI plants were more attractive to the egg parasitoid of BPH, Anagrus nilaparvatae than equally treated WT plants. The decreased attractiveness to BPH and enhanced attractiveness to the parasitoid of oeRCI plants correlated with higher levels of BPH-induced 2-heptanone and 2-heptanol, and 2-heptanone, respectively. Compared with oeRCI plants, WT plants had higher plant height and 1000-grain weight. These results indicate that OsRCI-1 is involved in herbivore-induced JA bursts and plays a role in plant defense and growth.
Assuntos
Hemípteros , Oryza , Animais , Ciclopentanos/metabolismo , Regulação da Expressão Gênica de Plantas , Hemípteros/fisiologia , Heptanol/metabolismo , Herbivoria , Oryza/metabolismo , Oxilipinas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismoRESUMO
When attacked by herbivores, plants emit volatiles to attract parasitoids and predators of herbivores. However, our understanding of the effect of plant volatiles on the subsequent behaviour of conspecific parasitoids when herbivores on plants are parasitized is limited. In this study, rice plants were infested with gravid females of the brown planthopper (BPH) Nilaparvata lugens for 24 hr followed by another 24 hr in which the BPH eggs on plants were permitted to be parasitized by their egg parasitoid, Anagrus nilaparvatae; volatiles from rice plants that underwent such treatment were less attractive to subsequent conspecific parasitoids compared to the volatiles from plants infested with gravid BPH females alone. Chemical analysis revealed that levels of JA and JA-Ile as well as of four volatile compounds-linalool, MeSA, α-zingiberene and an unknown compound-from plants infested with BPH and parasitized by wasps were significantly higher than levels of these compounds from BPH-infested plants. Laboratory and field bioassays revealed that one of the four increased chemicals-α-zingiberene-reduced the plant's attractiveness to the parasitoid. These results suggest that host plants can fine-tune their volatiles to help egg parasitoids distinguish host habitats with parasitized hosts from those without.
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Hemípteros/fisiologia , Interações Hospedeiro-Parasita , Himenópteros/fisiologia , Oryza/parasitologia , Defesa das Plantas contra Herbivoria/fisiologia , Compostos Orgânicos Voláteis/metabolismo , Vespas/fisiologia , Animais , Oryza/metabolismo , Oryza/fisiologia , Óvulo/parasitologia , Reação em Cadeia da Polimerase em Tempo RealRESUMO
MOTIVATION: DNA methylation signatures in rheumatoid arthritis (RA) have been identified in fibroblast-like synoviocytes (FLS) with Illumina HumanMethylation450 array. Since <2% of CpG sites are covered by the Illumina 450K array and whole genome bisulfite sequencing is still too expensive for many samples, computationally predicting DNA methylation levels based on 450K data would be valuable to discover more RA-related genes. RESULTS: We developed a computational model that is trained on 14 tissues with both whole genome bisulfite sequencing and 450K array data. This model integrates information derived from the similarity of local methylation pattern between tissues, the methylation information of flanking CpG sites and the methylation tendency of flanking DNA sequences. The predicted and measured methylation values were highly correlated with a Pearson correlation coefficient of 0.9 in leave-one-tissue-out cross-validations. Importantly, the majority (76%) of the top 10% differentially methylated loci among the 14 tissues was correctly detected using the predicted methylation values. Applying this model to 450K data of RA, osteoarthritis and normal FLS, we successfully expanded the coverage of CpG sites 18.5-fold and accounts for about 30% of all the CpGs in the human genome. By integrative omics study, we identified genes and pathways tightly related to RA pathogenesis, among which 12 genes were supported by triple evidences, including 6 genes already known to perform specific roles in RA and 6 genes as new potential therapeutic targets. AVAILABILITY AND IMPLEMENTATION: The source code, required data for prediction, and demo data for test are freely available at: http://wanglab.ucsd.edu/star/LR450K/ CONTACT: wei-wang@ucsd.edu or gfirestein@ucsd.edu SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
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Artrite Reumatoide , Metilação de DNA , Ilhas de CpG , Fibroblastos , Genoma Humano , Humanos , Análise de Sequência com Séries de OligonucleotídeosRESUMO
Tirumala limniace Cramer as an ornamental butterfly is utilized in butterfly garden, in this article we study their adult activities include flight, foraging, courtship, mating, and oviposition. We found that males spent 22.1% of its time flying, 14.1% foraging, 63.8% in courtship and mating. And females spent 30.8% of its time flying, 10.1% foraging, 57.1% in courtship and mating, and 2% ovipositing. Adults emerged from pupae when temperatures were above 23.5°C and eclosion took only â¼1 min, typically followed by a small amount of flight on the first day. Flight activity peaked from the ninth to the thirteenth day after eclosion, and there were two daily peak flight times: 10:00-13:00 and 15:00-18:00. The peak of flower-visiting activity was from the eighth to the thirteenth day after eclosion, and there were two daily peak foraging times: 11:00-12:00 and 16:00-17:00. Flight and foraging frequency and time were positively correlated and both were closely related to temperature, with very little flight or foraging below 18°C and an increase at temperatures above 22°C. Courtship and mating took place on the sixth day after eclosion, while oviposition occurred the following day. Oviposition occurred over 8 d, and the shortest time of a single oviposition was 2 s. The average life expectancy of males was 16.5 d, while that of females was 15 d.
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Borboletas/fisiologia , Voo Animal , Animais , Comportamento Apetitivo , Borboletas/crescimento & desenvolvimento , Ritmo Circadiano , Corte , Feminino , Flores , Masculino , Oviposição/fisiologia , Comportamento Sexual Animal , TemperaturaRESUMO
In the post-epidemic era, the restart of China's inbound tourism is imminent. However, there are gaps in our current understanding of how distance perception dynamically affects inbound tourism in China. In order to understand the past patterns of inbound tourism in China, we mapped the data of 61 countries of origin from 2004 to 2018 into a dynamic expanding gravity model to understand the effects of cultural distance, institutional distance, geographical distance, and economic distance on inbound tourism in China and revealed the dynamic interaction mechanism of non-economic distance perception on inbound tourism in China. Our research results show that cultural distance has a positive impact on China's inbound tourism, while institutional distance has a negative impact. The significant finding is that the dynamic interaction of the above two kinds of perceived distance can still have a positive impact on China's inbound tourism. Its practical significance is that it can counteract the influence of institutional distance by strengthening the cultural distance. Generally speaking, geographical distance and institutional distance restrict China's inbound tourism flow, while cultural distance, economic distance, and interactive perceptual distance promote China's inbound tourism flow.
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Turismo , China , Humanos , Modelos Teóricos , Percepção de Distância , Viagem/economia , GravitaçãoRESUMO
Butterflies have the ability to learn to associate olfactory information with abundant food sources during foraging. How the co-occurrence of both food and food odours affects the learning behaviour of adults and whether butterflies perceive the odour of their surroundings and develop a preference for that odour during the pupal stage have rarely been tested. We examined the effect of experience with food odour components (α-pinene and ethyl acetate) during the pupal and adult stages on the foraging behaviour of the flower-visiting butterfly Tirumala limniace. We found that α-pinene exposure during the pupal stage changed the foraging preference of newly emerged adults. T. limniace exhibits olfactory learning in the adult stage, and adult learning may influence their previous pupal memory. Moreover, adults' odour preference did not continue to increase over multiple training times. The learning ability of adults for floral odours (α-pinene) was greater than that for non-floral odours (ethyl acetate). In contrast to previous studies, we found that males learned odours more efficiently than females did. This could be attributed to differences in antennal sensilla, affecting sensitivity to compounds and nectar demand between males and females. Our study provides further insight into how olfactory learning helps flower-visiting butterflies use food odours to forage better.
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Green leaf volatiles (GLVs) play pivotal roles in plant anti-herbivore defense. This study investigated whether the rice 13-lipoxygense gene OsRCI-1 is involved in GLV production and plant defense in rice. The overexpression of OsRCI-1 (oeRCI lines) in rice resulted in increased wound-induced levels of two prominent GLVs, cis-3-hexen-1-ol and cis-3-hexenal. In a previous study, we found that the overexpression of OsRCI-1 reduced the colonization by the rice brown planthopper (BPH, Nilaparvata lugens) but increased the attractiveness to the egg parasitoid Anagrus nilaparvatae compared to wild-type (WT) plants. This study found that when cis-3-hexen-1-ol, but not cis-3-hexenal, was added to WT plants, it could change the BPH's colonization preference, i.e., more BPHs preferred to colonize the oeRCI lines. The exogenous application of cis-3-hexen-1-ol or cis-3-hexenal to BPH-infested WT plants could weaken or overturn the preference of A. nilaparvatae for oeRCI lines. However, field experiments revealed that only cis-3-hexenal was attractive to the parasitoid and increased the parasitism rates of BPH eggs. These results indicate that OsRCI-1 is involved in rice GLV production and therefore modulates both direct and indirect defense in rice.
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The Multi-Angle Imager for Aerosols (MAIA), supported by NASA and the Italian Space Agency, is planned for launch into space in 2025. As part of its mission goal, outputs from a chemical transport model, the Unified Inputs for Weather Research and Forecasting Model coupled with Chemistry (UI-WRF-Chem), will be used together with satellite data and surface observations for estimating surface PM2.5. Here, we develop a method to improve UI-WRF-Chem with surface observations at the U.S. embassy in Ethiopia, one of MAIA's primary target areas in east Africa. The method inversely models the diurnal profile and amount of anthropogenic aerosol and trace gas emissions. Low-cost PurpleAir sensor data are used for validation after applying calibration functions obtained from the collocated data at the embassy. With the emission updates in UI-WRF-Chem, independent validation for February 2022 at several different PurpleAir sites shows an increase in the linear correlation coefficients from 0.1-0.7 to 0.6-0.9 between observations and simulations of the diurnal variation of surface PM2.5. Furthermore, even by using the emissions optimized for February 2021, the UI-WRF-Chem forecast for March 2022 is also improved. Annual update of monthly emissions via inverse modeling has the potential and is needed to improve MAIA's estimate of surface PM2.5.
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As a novel strategy for in vivo visualization tracking and monitoring, carbon dots (CDs) emitting long wavelengths (LW, 600-950 nm) have received tremendous attention due to their deep tissue penetration, low photon scattering, satisfactory contrast resolution and high signal-to-background ratios. Although, the mechanism of CDs emitting LW remains controversial and what properties are best for in vivo visualization have not been specifically elucidated, it is more conducive to the in vivo application of LW-CDs through rational design and ingenious synthesis based on the appreciation of the luminescence mechanism. Therefore, this review analyzes the current tracer technologies applied in vivo and their advantages and disadvantages, with emphasis on the physical mechanism of emitting LW fluorescence for in vivo imaging. Subsequently, the general properties and merits of LW-CDs for tracking and imaging are summarized. More importantly, the factors affecting the synthesis of LW-CDs and its luminescence mechanism are highlighted. Simultaneously, the application of LW-CDs for disease diagnosis, integration of diagnosis and therapy are summarized. Finally, the bottlenecks and possible future directions of LW-CDs in visualization tracking and imaging in vivo are detailly discussed.
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Pontos Quânticos , Medicina de Precisão , Carbono , Luminescência , FluorescênciaRESUMO
Moso bamboo is considered a potential species for heavy metal (HM) phytoremediation; however, the effect of intercropping on rhizosphere and phytoextraction remains to be elucidated. We comparatively investigated rhizobacteria, soil properties, and phytoextraction efficiency of monoculture and intercropping of Moso bamboo and Sedum plumbizincicola in Cu/Zn/Cd-contaminated soil. Compared with monocultures, intercropping increased the bacterial α-diversity indices (Shannon, Chao1) and the number of biomarkers. Intercropping reduced the contents of soil organic matter (SOM), available nutrients, and Cd and Cu in rhizosphere soils, and reduced the Cd and Zn contents in tissues of sedum. By contrast, Cd and Zn contents in tissues of bamboo increased, and the increase of organic acid in root exudates from intercropping could facilitate the HM absorption. The total amount of Cu, Zn, and Cd removed from the soil in intercropping system was 1.2, 1.9, and 1.8 times than those in monoculture bamboo, respectively. The abundances of Proteobacteria, Acidobacteria, Verrucomicrobia and Actinobacteria were higher in intercropping, playing an important role in soil nutrient cycles and HM remediation. These bacterial communities were closely correlated (P < 0.01) with SOM, available nitrogen, available phosphorus, and HMs. The results suggested this intercropping pattern can increase HM removal efficiency from polluted soils.
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Metais Pesados , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Metais Pesados/análise , Rizosfera , Solo , Poluentes do Solo/análiseRESUMO
A black hole X-ray binary produces hard X-ray radiation from its corona and disk when the accreting matter heats up. During an outburst, the disk and corona co-evolves with each other. However, such an evolution is still unclear in both its geometry and dynamics. Here we report the unusual decrease of the reflection fraction in MAXI J1820+070, which is the ratio of the coronal intensity illuminating the disk to the coronal intensity reaching the observer, as the corona is observed to contrast during the decay phase. We postulate a jet-like corona model, in which the corona can be understood as a standing shock where the material flowing through. In this dynamical scenario, the decrease of the reflection fraction is a signature of the corona's bulk velocity. Our findings suggest that as the corona is observed to get closer to the black hole, the coronal material might be outflowing faster.
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Butterflies use multiple signals, including visual, olfactory and tactile cues, to identify same- and opposite-sex individuals during courtship. In this study of the sexually dimorphic butterfly Cethosia cyane cyane, we explored the roles of visual and olfactory cues in conspecific mate recognition during courtship. Our results showed that males took the initiative in actively chasing females during courtship using only visual cues. Males could distinguish the gender of conspecifics using visual cues alone. The size and color of the wings differ significantly between the sexes. Behavioral assays showed that males visually recognized females not by wing size, but by their sexually specific wing color. The movement pattern of the model also exerted some influence on male courtship chasing behavior. A total of 21 volatiles were detected in the bodies of adults, but only cedrol played a role in the process of male recognition of females at close range. Therefore, males rely on both visual and olfactory cues to distinguish females during courtship. Visual cues play a major role in attracting males at the beginning of the courtship chase, while olfactory cues play a role in accurately identifying partners at close range.