Broadband source-driven resonant micro-optic gyroscope based on a multi-turn waveguide-type ring resonator.

The resonant micro-optic gyroscope (RMOG) is one of the most promising candidates for chip-scale optoelectronic gyroscopes. A broadband source-driven RMOG based on a multi-turn waveguide-type ring resonator (WRR) has been proposed and demonstrated. The theoretical sensitivity is enhanced with the multi-turn structure, while the parasitic backscattering can be resolved by the use of the broadband source, thus greatly improving the long-term bias stability of the RMOG. We also reduce the relative intensity noise (RIN)-induced error of the broadband source at the gyro output by optimizing the number of loop turns of the WRR, and improve the angle random walk (ARW) by 4.8 dB compared with the case of a single-turn WRR. Finally, a bias stability of 1°/h is obtained with a 5-turn WRR of 4.05 cm diameter, achieving the tactical-grade resolution. To the best of our knowledge this is the best result reported to date for an RMOG of similar size.

Ultrahigh-resolution and ultra-simple fiber-optic sensor with resonant Sagnac interferometer.

The resonant fiber-optic sensor (RFOS) is well known for its high sensing resolution but usually suffers from high cost and system complexity. In this Letter, we propose an ultra-simple white-light-driven RFOS with a resonant Sagnac interferometer. By superimposing the output of multiple equivalent Sagnac interferometers, the strain signal is amplified during the resonance. A 3 × 3 coupler is employed for demodulation, by which the signal under test can be read out directly without any modulation. With 1 km delay fiber and ultra-simple configuration, a strain resolution of 28f ε/Hz at 5 kHz is demonstrated in the experiment, which is among the highest, to the best of our knowledge, resolution optical fiber strain sensors.

Navigation-grade three-axis resonant fiber-optic gyroscope employing a multiplexed source.

A three-axis gyroscope is a vital component of an inertial measurement unit that can measure the rotation rates in three directions simultaneously. A novel three-axis resonant fiber-optic gyroscope (RFOG) configuration with a multiplexed broadband light source is proposed and demonstrated. The output light from the two vacant ports of the main gyroscope is reused as drive sources for the other two axial gyroscopes, which effectively improve the power utilization of the source. The interference between different axial gyroscopes is effectively avoided by optimizing the lengths of three fiber-optic ring resonators (FRRs) rather than by inserting other optical elements in the multiplexed link. With the optimal lengths, the influence of the input spectrum on the multiplexed RFOG is suppressed and a theoretical temperature dependence of the bias error as low as 1.08 × 10-4 °/h/°C is obtained. Finally, a navigation-grade three-axis RFOG is demonstrated with a fiber coil length of ∼100 m for each FRR.

A "trained immunity" inducer-adjuvanted nanovaccine reverses the growth of established tumors in mice.

Innate immune cells are critical in antitumor immune surveillance and the development of antitumor adaptive cellular immunity. Trained innate immune cells demonstrate immune memory-like characteristics, producing more vigorous immune responses to secondary homologous or heterologous stimuli. This study aimed to investigate whether inducing trained immunity is beneficial when using a tumor vaccine to promote antitumor adaptive immune responses. A biphasic delivery system was developed with the trained immunity inducer Muramyl Dipeptide (MDP) and specific tumor antigen human papillomavirus (HPV) E7 peptide encapsulated by poly(lactide-co-glycolide)-acid(PLGA) nanoparticles (NPs), and the NPs along with another trained immunity agonist, ß-glucan, were further embedded in a sodium alginate hydrogel. The nanovaccine formulation demonstrated a depot effect for E7 at the injection site and targeted delivery to the lymph nodes and dendritic cells (DCs). The antigen uptake and maturation of DCs were significantly promoted. A trained immunity phenotype, characterized by increased production of IL-1ß, IL-6, and TNF-α, was induced in vitro and in vivo in response to secondary homologous or heterologous stimulation. Furthermore, prior innate immune training enhanced the antigen-specific INF-γ-expressing immune cell response elicited by subsequent stimulation with the nanovaccine. Immunization with the nanovaccine completely inhibited the growth of TC-1 tumors and even abolished established tumors in mice. Mechanistically, the inclusion of ß-glucan and MDP significantly enhanced the responses of tumor-specific effector adaptive immune cells. The results strongly suggest that the controlled release and targeted delivery of an antigen and trained immunity inducers with an NP/hydrogel biphasic system can elicit robust adaptive immunity, which provides a promising tumor vaccination strategy.

Exploiting immunostimulatory mechanisms of immunogenic cell death to develop membrane-encapsulated nanoparticles as a potent tumor vaccine.

Vaccine is one of the most promising strategies for cancer immunotherapy; however, there are no therapeutic cancer vaccine achieving significant clinical efficacy till now. The main limiting factors include the immune suppression and escape mechanisms developed by tumor and not enough capacity of vaccines to induce a vigorous anti-tumor immunity. This study aimed to develop a strategy of membrane-based biomimetic nanovaccine and investigate the immunological outcomes of utilizing the unique immunostimulatory mechanisms derived of immunogenic cell death (ICD) and of fulfilling a simultaneous nanoscale delivery of a highlighted tumor antigen and broad membrane-associated tumor antigens in the vaccine design. TC-1 tumor cells were treated in vitro with a mixture of mitoxantrone and curcumin for ICD induction, and then chitosan (CS)-coated polylactic co-glycolic acid (PLGA) nanoparticles loaded with HPV16 E744-62 peptides were decorated with the prepared ICD tumor cell membrane (IM); further, the IM-decorated nanoparticles along with adenosine triphosphate (ATP) were embedded with sodium alginate (ALG) hydrogel, And then, the immunological features and therapeutic potency were evaluated in vitro and in vivo. The nanovaccine significantly stimulated the migration, antigen uptake, and maturation of DCs in vitro, improved antigen lysosome escape, and promoted the retention at injection site and accumulation in LNs of the tumor antigen in vivo. In a subcutaneously grafted TC-1 tumor model, the therapeutic immunization of nanovaccine elicited a dramatical antitumor immunity. This study provides a strategy for the development of tumor vaccines.

Pico-strain resolution fiber-optic sensor with white-light interferometry.

The fiber Bragg grating (FBG) sensor is well known for simple fabrication, absolute measurement, inherent multiplexing capability, etc. To date, most FBG sensors that use a broadband light source for demodulation can only achieve resolution at the µÉ› level. In this Letter, we propose a white-light-driven self-reference sensing system with FBGs, using a Mach-Zehnder interferometer (MZI) and a white light source, to realize multiplexed high-resolution vibration sensing with a very simple system configuration. A strain resolution of 35p ε/Hz at 1 kHz is demonstrated, which is several orders of magnitude better than the current FBG sensor systems with white light sources. The performance of the sensing system is analyzed, and multiplexing capability is also experimentally evaluated; there is no observable cross talk.

White-light-driven resonant fiber-optic gyro based on round trip filtering scheme.

As the second generation of the fiber-optic gyro (FOG), a resonant FOG (RFOG) appears as a very viable candidate for a miniaturized optical gyro. However, due to the impediment of laser-induced parasitic noise and system complexity, the actual performance of the RFOG is well below expectations. This paper proposes a novel, to the best of our knowledge, RFOG which is driven by broadband white light rather than a narrow linewidth laser. The fiber-optic ring resonator (FRR) works as a filter, and the rotation under detection is read out from the round trip loss of the FRR. The parasitic noise is effectively avoided due to the low coherence light, and the measuring resolution can be thus improved. In the experiment, a bias instability of 0.012 ∘/h is demonstrated with a 100-m fiber coil and a very simple structure. The proposed method would be a big step forward for making the RFOG practical with high performance and low cost.

Femto-strain resolution fiber-optic sensing with an ultra-simple white-light round trip filtering method.

In the past decade, laser-driven resonant fiber-optic sensors (RFOSs) have been reported touching their ultimate resolution limit. The practicability of these high-performance sensors is, however, discounted because of high system complexity and dependence on narrow-linewidth lasers. In this paper, a novel, to the best of our knowledge, white-light-driven RFOS is established based on a round trip filtering (RTF) method. Via measuring the RTF loss of an add-drop fiber ring resonator (FRR) sensor, strain signal can be read out with an ultra-simple open-loop configuration. In the sensing experiment, even a resolution of several femto-strain around 1 kHz is demonstrated, representing the highest resolution level of RFOS to date. Thanks to the obvious superiority in both resolution, simplicity, and cost over traditional laser-driven RFOSs, the proposed white-light-driven RFOS is believed to be a milestone in the development of fiber-optic strain sensors.

Quadrature photonic spatial Ising machine.

As a computing accelerator, a large-scale photonic spatial Ising machine has great advantages and potential due to its excellent scalability and compactness. However, the current fundamental limitation of a photonic spatial Ising machine is the configuration flexibility for problem implementation in the accelerator model. Arbitrary spin interactions are highly desired for solving various non-deterministic polynomial (NP)-hard problems. In this paper, we propose a novel quadrature photonic spatial Ising machine to break through the limitation of the photonic Ising accelerator by synchronous phase manipulation in two sections. The max-cut problem solution with a graph order of 100 and density from 0.5 to 1 is experimentally demonstrated after almost 100 iterations. Our work suggests flexible problem solving by the large-scale photonic spatial Ising machine.

Reduction of relative intensity noise in a broadband source-driven RFOG using a high-frequency modulation technique.

A broadband source-driven resonant fiber-optic gyroscope (RFOG) can reduce coherence-related noise, thus achieving a better sensitivity with a much simpler configuration than the traditional system with a coherent source. Its detection sensitivity, however, is still limited by the excess relative intensity noise (RIN) of the broadband source. In this paper, the RIN error mechanism in this broadband source-driven RFOG is revealed and countermeasures are presented. We demonstrate that the use of a high-finesse fiber-optic ring resonator and a high-frequency modulation-demodulation technique can reduce the RIN-induced error. It is indicated that the optimal modulation parameters can provide a RIN-induced error reduction of 6.1 dB, allowing the broadband source-driven RFOG to operate near the shot-noise-limited theoretical sensitivity. With the optimal high-frequency modulation-demodulation technique, an angle random walk of 0.0013°/√h is achieved with a 200-m-long fiber-optic ring resonator of 7.6 cm diameter. This is the best result reported to date, to the best of our knowledge, for fiber-optic gyroscopes of this size.

Quasi-distributed fiber-optic acoustic sensor based on a low coherence light source.

A quasi-distributed acoustic sensor using in-line weak reflectors and a low coherence light source is presented. The dynamic strain is retrieved from the phase change of the two interfering light beams reflected by the same weak reflector. In the experiments, two vibrations at different channels along a weak reflector array are successfully detected simultaneously. A strain resolution of 50 pɛ/H z with 20-m interval is achieved in experiments, and no cross talk is observed. With simple system configuration and low cost, this approach provides a new, to the best of our knowledge, solution for quasi-distributed acoustic sensing.

Quasi-distributed fiber-optic acoustic sensor based on a low coherence light source: publisher's note.

This publisher's note contains a correction to Opt. Lett.47, 3780 (2022)10.1364/OL.464020.

Single-exposure multi-wavelength diffraction imaging with blazed grating.

Multi-wavelength diffraction imaging is a lensless, high-resolution imaging technology. To avoid multiple exposures and enable high-speed data collection, here an innovative setup for the single-exposure multi-wavelength diffraction imaging based on a blazed grating is proposed. Since the blazed angle varies with the wavelength, the diffraction patterns for the individual wavelengths can be separated from each other and recorded in a single measurement at one time. A method of high-precision position alignment between different wavelength patterns is proposed in our system to achieve good image quality and high resolution. Experiments on a phase-only USAF resolution target and biological samples were carried out to verify the effectiveness of our proposed method. This proposed setup has such advantages as a simpler structure, fast recording, and algorithm robustness.

Complete modal decomposition of a few-mode fiber based on ptychography technology.

An exact modal decomposition method plays an important role in revealing the modal characteristics of a few-mode fiber, and it is widely used in various applications ranging from imaging to telecommunications. Here, ptychography technology is successfully used to achieve modal decomposition of a few-mode fiber. In our method, the complex amplitude information of the test fiber can be recovered by ptychography, and then the amplitude weight of each eigenmode and the relative phase between different eigenmodes can be easily calculated by modal orthogonal projection operations. In addition, we also propose a simple and effective method to realize coordinate alignment. Numerical simulations and optical experiments validate the reliability and feasibility of the approach.

Performance of a resonant fiber-optic gyroscope based on a broadband source.

A resonant fiber-optic gyroscope (RFOG) based on a broadband source can avoid the fundamental drawback of coherence detection processing while possessing the greater sensitivity afforded by the finesse of the fiber-optic ring resonator. In this paper, the basic operation principle is presented and demonstrated in detail, and various noise sources, as well as the temperature effect encountered in this broadband source-driven RFOG, are studied and analyzed. Then a combined modulation technique is proposed to suppress the residual backscattering noise. To further reduce the effect of temperature transience, an asymmetric fiber ring resonator is designed. In the experiment, a bias stability of 0.01°/h is successfully demonstrated with a 100 m-long fiber ring resonator of 8 cm diameter in a laboratory environment without temperature control.

Use of small pulmonary vascular alterations to identify different types of pulmonary hypertension: a quantitative computed tomography analysis.

BACKGROUND: The morphological alterations of small pulmonary vessels measured by computed tomography (CT) is increasingly used in evaluation of suspected pulmonary hypertension (PH). OBJECTIVE: To investigate the significance alterations of quantitative assessment of small pulmonary vessels on chest CT in distinguishing different types of PH and their severity. METHODS: We retrospectively analyzed a dataset of 120 healthy controls (HCs) and 91 PH patients, including 34 patients with connective tissue diseases-related PH (CTD-PH), 26 patients with idiopathic pulmonary arterial hypertension (iPAH), and 31 patients with chronic obstructive pulmonary disease-related PH (COPD-PH). The CTD-PH patients were divided into mild to moderate PH (CTD-LM-PH) group (n = 17) and severe PH (CTD-S-PH) group (n = 17). A total of 53 CTD patients without PH (CTD-nPH) were enrolled for comparison with the CTD-PH. We measured the cross-sectional area of small pulmonary vessels < 5 mm2 (%CSA <5) and between 5-10 mm2 (%CSA5-10) as a percentage of total lung area among the populations included above and compared %CSA in different types of PH groups and HCs group. The mean pulmonary arterial pressure (mPAP) was measured by right heart catheterization. RESULTS: The %CSA5-10 of COPD-PH, CTD-PH, and iPAH patients increased (0.21±0.09, 0.49±0.20 and 0.61±0.20, p < 0.02) sequentially, while the %CSA <5 of CTD-PH, iPAH, and COPD-PH patients decreased (0.79±0.65, 0.65±0.38 and 0.52±0.27, p < 0.05) sequentially. The %CSA5-10 was significantly higher in CTD-S-PH patients than CTD-LM-PH patients and CTD-nPH patients (0.51±0.21, 0.31±0.15 and 0.28±0.12, p < 0.01). The %CSA5-10 was positively correlated with mPAP in the CTD-PH group. CONCLUSIONS: The quantitative parameters %CSA <5 and %CSA5-10 assessed by chest CT are useful for distinguishing different types of PH. In addition, the %CSA5-10 can provide information for identification of CTD-PH severity.

Resonant fiber-optic strain and temperature sensor achieving thermal-noise-limit resolution.

In the area of fiber-optic sensors (FOSs), the past decade witnessed great efforts to challenge the thermal-noise-level sensing resolution for passive FOS. Several attempts were reported claiming the arrival of thermal-noise-level resolution, while the realization of thermal-noise-level resolution for passive FOSs is still controversial and challenging. In this paper, an ultrahigh-resolution FOS system is presented with a sensing resolution better than existing high-resolution passive FOSs. A fiber Fabry-Perot interferometer as the sensing element is interrogated with an ultra-stable probe laser by using the Pound-Drever-Hall technique. Both strain and temperature measurements are carried out to validate the performance of the sensor. The measured noise floor agrees with the theoretical thermal noise level very well.

High-resolution multi-wavelength lensless diffraction imaging with adaptive dispersion correction.

Multi-wavelength imaging diffraction system is a promising phase imaging technology due to its advantages of no mechanical movement and low complexity. In a multi-wavelength focused system, spectral bandwidth and dispersion correction are critical for high resolution reconstruction. Here, an optical setup for the multi-wavelength lensless diffraction imaging system with adaptive dispersion correction is proposed. Three beams with different wavelengths are adopted to illuminate the test object, and then the diffraction patterns are recorded by a image sensor. The chromatic correction is successfully realized by a robust refocusing technique. High-resolution images can be finally retrieved through phase retrieval algorithm. The effectiveness and reliability of our method is demonstrated in numerical simulation and experiments. The proposed method has the potential to be an alternative technology for quantitative biological imaging.

Loss of GATA4 C-Terminus by p.S335X Mutation Modulates Coronary Artery Vascular Smooth Muscle Cell Phenotype.

Coronary artery disease (CAD) has been the leading cause of morbidity and mortality worldwide, and its pathogenesis is closely related with the proliferation and migration of vascular smooth muscle cell (VSMC). We previously reported a truncated GATA4 protein lacking C-terminus induced by p.S335X mutation in cardiomyocyte from ventricular septal defect (VSD) patients. However, it is still unclear whether GATA4 p.S335X mutation could influence the development of CAD. GATA4 wild-type (WT) and p.S335X mutant (MU) overexpression plasmids were constructed and transfected transiently into rat coronary artery smooth muscle cell (RCSMC) to observe the proliferative and migratory abilities by MTS and wound healing assay, respectively. PCR array was used to preliminarily detect the expression of phenotypic modulation-related genes, and QRT-PCR was then carried out to verify the screened differentially expressed genes (DEGs). The results showed that, when stimulated by fetal bovine serum (10%) for 24 h or tumor necrosis factor-α (10 or 30 ng/ml) for 10 or 24 h, deletion of GATA4 C-terminus by p.S335X mutation in GATA4 enhanced the proliferation of RCSMC, without alteration of the migration capability. Twelve DEGs, including Fas, Hbegf, Itga5, Aimp1, Cxcl1, Il15, Il2rg, Il7, Tnfsf10, Il1r1, Irak1, and Tlr3, were screened and identified as phenotypic modulation-related genes. Our data might be beneficial for further exploration regarding the mechanisms of GATA4 p.S335X mutation on the phenotypic modulation of coronary VSMC.

Development of Real-Time Time Gated Digital (TGD) OFDR Method and Its Performance Verification.

Distributed acoustic sensing (DAS) in optical fibers detect dynamic strains or sound waves by measuring the phase or amplitude changes of the scattered light. This contrasts with other distributed (and more conventional) methods, such as distributed temperature (DTS) or strain (DSS), which measure quasi-static physical quantities, such as intensity spectrum of the scattered light. DAS is attracting considerable attention as it complements the conventional distributed measurements. To implement DAS in commercial applications, it is necessary to ensure a sufficiently high signal-noise ratio (SNR) for scattered light detection, suppress its deterioration along the sensing fiber, achieve lower noise floor for weak signals and, moreover, perform high-speed processing within milliseconds (or sometimes even less). In this paper, we present a new, real-time DAS, realized by using the time gated digital-optical frequency domain reflectometry (TGD-OFDR) method, in which the chirp pulse is divided into overlapping bands and assembled after digital decoding. The developed prototype NBX-S4000 generates a chirp signal with a pulse duration of 2 µs and uses a frequency sweep of 100 MHz at a repeating frequency of up to 5 kHz. It allows one to detect sound waves at an 80 km fiber distance range with spatial resolution better than a theoretically calculated value of 2.8 m in real time. The developed prototype was tested in the field in various applications, from earthquake detection and submarine cable sensing to oil and gas industry applications. All obtained results confirmed effectiveness of the method and performance, surpassing, in conventional SM fiber, other commercially available interrogators.