Dynamic expression and functional analysis of circRNA in granulosa cells during follicular development in chicken.

BACKGROUND: Circular RNA (circRNA) is a type of noncoding RNA involved in a variety of biological processes, especially in post-transcriptional regulation. The granulosa cells of follicles play a determining role in ovarian development. However, the function of circRNA in chicken follicles is unclear. To better understand the molecular mechanism underlying follicular development and granulosa cell function, we performed a strategy of second-generation sequencing and linear RNA depletion for granulosa cells from small yellow follicles (SYF, 5-8 mm), the smallest hierarchal follicles (F6, 9-12 mm), and the largest hierarchal follicles (F1, ~ 40 mm). RESULTS: We predicted a total of 11,642 circRNAs that distributed on almost all chromosomes. The majority of the splice lengths of circRNAs were 200-500 nt and mainly produced from intron and CDS regions. During follicle growth, differentially expressed (DE) circRNAs showed dynamic changes which were tissue- and stage-specific. The host genes of DE circRNAs were functionally enriched in GTPase activity and several pathways involved in reproduction. Moreover, bioinformatic prediction analysis for circRalGPS2 demonstrated that circRNAs from the same genes may share common miRNA to act as a sponge. The predicted target genes were enriched in various biological processes including cognition, cell communication, and regulation of signaling, and several pathways related to reproduction such as tight junction, oocyte meiosis, progesterone-mediated oocyte maturation, and GnRH signaling. CONCLUSIONS: This study provides a starting point for further experimental investigations into chicken circRNAs and casts a light on the understanding of follicle development.

Polymorphism identification in GDF9 gene and its association analysis with reproduction traits in Jinghai Yellow chicken.

GDF9 (growth differentiation factor 9) belongs to the transforming growth factor-ß (TGF-ß) superfamily and plays an irreplaceable role in female fertility. To reveal its genetic effects on productivity performance in chickens, 373 Jinghai Yellow chickens were chosen randomly to detect SNPs in GDF9 by PCR-SSCP and DNA sequencing methods. Eventually, four SNPs (g.2053G > A, g.2275T > C, g.2338C > T, g.2420T > C) in total had been detected. Amongst them, g.2420T > C was first found significantly associated with reproduction trait in chickens and heterozygous type C2T2 had higher average egg weight at 300 days of age (AEWD300) than T2T2 (p < 0.01). Least squares analysis showed that age at first laying (AFE) of H1 and H1H1 chickens were significantly earlier than that of H7 and H7H7 ones, respectively (p < 0.05). H1H5 hens showed higher AEWD300 than H4H7 ones (p < 0.05). For total egg number at 300 days of age (END300), mean of H5H5 was significantly higher than that of H4H4 (p < 0.05). Hence, the study suggested that hybrid vigor at g.2420T > C could be utilized in practice. H1H1, H1H5 and H5H5 could be the dominant diplotypes for chicken breeding. The study may contribute to the breeding progress of productive chickens and supply reference for oviparous animal production practice.