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1.
Materials (Basel) ; 17(10)2024 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-38793269

RESUMO

316L stainless steel pipes are widely used in the storage and transportation of low-temperature media due to their excellent low-temperature mechanical properties and corrosion resistance. However, due to their low thermal conductivity and large coefficient of linear expansion, they often lead to significant welding residual tensile stress and thermal cracks in the weld seam. This also poses many challenges for their secure and reliable applications. In order to effectively control the crack defects caused by stress concentration near the heat-affected zone of the weld, this paper establishes a thermal elastoplastic three-dimensional finite element (FE) model, constructs a welding heat source, and simulates and studies the influence of process parameters on the residual stress around the pipeline circumference and axial direction in the heat-affected zone. Comparison and verification were conducted using simulation and experimental methods, respectively, proving the rationality of the finite element model establishment. The axial and circumferential residual stress distribution obtained by the simulation method did not have an average deviation of more than 30 MPa from the numerical values obtained by the experimental method. This study also considers the effects of welding energy, welding speed, and welding start position on the pipe's circumferential and axial residual stress laws. The results indicate that changes in welding energy and welding speed have almost no effect on the longitudinal residual stress but have a more significant effect on the transverse residual stress. The maximum transverse residual stress is reached at a welding energy of 1007.4~859.3 J/mm and a welding speed of 6.6 mm/s. Various interlayer arc-striking deflection angles can impact the cyclic phase angle of the transverse residual stress distribution in the seam center, but they do not alter its cyclic pattern. They do influence the amplitude and distribution of the longitudinal residual stress along the circumference. The residual stress distribution on the surface of the pipe fitting is homogenized and improved at 120°.

2.
Materials (Basel) ; 15(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36143566

RESUMO

Due to its extreme service conditions, low-temperature pressure piping often needs post-welding stress measurement and control. Aiming at the phenomenon of local stress concentration in welded 316L pipes, this study used ultrasound to regulate the stress in the welded area at different times during and after the multi-layer welding of the pipeline butt joint for different time lengths. Mechanical properties such as tensile strength and hardness were tested for each comparison group, and the microcrystalline phases of the weld and its surrounding microstructure were analyzed. The transverse and longitudinal surface residual stresses of each comparison group were measured. The influence of high-energy ultrasound on the surface temperature field during and after welding was analyzed. The experimental results show that ultrasonic wave regulation can speed up heat exchange and radiation in the weld zone (WZ), refine the grains in the WZ, heat-affected zone (HAZ) and fusion zone (FZ) to some extent and reduce and homogenize residual stress to a certain degree. In the 120 mm area of the weld center, the residual stress measured after the mid-welding regulation was smaller than that of any other comparison group. This regulation result was the best, followed by that of hot regulation and finally that of offline regulation. The tensile strengths obtained by the mid-welding regulation and post-welding hot regulation of this group were the best, increasing by 17.2% and 24.3%, respectively, compared with the untreated groups.

3.
Lab Chip ; 12(20): 3907-13, 2012 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-22836582

RESUMO

Genetic alternations can serve as highly specific biomarkers to distinguish fatal bacteria or cancer cells from their normal counterparts. However, these mutations normally exist in very rare amount in the presence of a large excess of non-mutated analogs. Taking the notorious pathogen E. coli O157:H7 as the target analyte, we have developed an agarose droplet-based microfluidic ePCR method for highly sensitive, specific and quantitative detection of rare pathogens in the high background of normal bacteria. Massively parallel singleplex and multiplex PCR at the single-cell level in agarose droplets have been successfully established. Moreover, we challenged the system with rare pathogen detection and realized the sensitive and quantitative analysis of a single E. coli O157:H7 cell in the high background of 100,000 excess normal K12 cells. For the first time, we demonstrated rare pathogen detection through agarose droplet microfluidic ePCR. Such a multiplex single-cell agarose droplet amplification method enables ultra-high throughput and multi-parameter genetic analysis of large population of cells at the single-cell level to uncover the stochastic variations in biological systems.


Assuntos
Escherichia coli K12/genética , Escherichia coli O157/genética , Técnicas Analíticas Microfluídicas , Reação em Cadeia da Polimerase , Sefarose/química , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Reação em Cadeia da Polimerase/instrumentação , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade
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