Interferon-γ regulates the function of mesenchymal stem cells from oral lichen planus via indoleamine 2,3-dioxygenase activity.

BACKGROUND: Little is known about mesenchymal stem cells (MSCs) in normal or inflammatory oral mucosal tissues, such as in oral lichen planus (OLP). Our objectives were to identify, isolate, and characterize MSCs from normal human oral mucosa and OLP lesions, and to evaluate indoleamine 2,3 dioxygenase (IDO) activity in mediating immunomodulation of MSCs from these tissues. METHODS: Expressions of MSCs-related markers were examined in isolated cells by flow cytometry. Self-renewal and multilineage differentiations were studied to characterize these MSCs. Interferon-γ (IFN-γ), IDO, and STRO-1 were assessed by immunofluorescence. MSCs from oral mucosa and OLP or IFN-γ-pretreated MSCs were co-cultured with allogeneic mixed lymphocyte reaction assays (MLR). Proliferation and apoptosis of MLR or MSCs were detected by CCK8 and the annexin V-FITC apoptosis detection kit, respectively. IDO expression and activity were measured by real-time PCR, Western blotting, and high-performance liquid chromatography. RESULTS: Isolated cells from oral mucosa and OLP expressed MSC-related markers STRO-1, CD105, and CD90 but were absent for hematopoietic stem cell markers CD34. Besides, they all showed self-renewal and multilineage differentiation capacities. MSCs in OLP presented STRO-1/IDO+ phenotype by immunofluorescence. MSCs and IFN-γ-pretreated MSCs could inhibit lymphocyte proliferation via IDO activity, but not via cell apoptosis. Long-term IFN-γ could also inhibit MSC proliferation via IDO activity. CONCLUSIONS: Mesenchymal stem cells can be isolated from human oral mucosa and OLP tissues. Besides self-renewal and multilineage differentiation properties, these cells may participate in immunomodulation mediated by IFN-γ via IDO activity in human OLP.

Efficacy of systemic use of antibiotics as an adjunct to nonsurgical treatment of peri-implantitis: a meta-analysis of randomized controlled trials.

OBJECTIVES: The role of antibiotics as an adjunct to nonsurgical peri-implantitis treatment approaches has not reached a consensus. This meta-analysis aimed to review the adjunctive effect of systemic use of metronidazole and amoxicillin in patients with peri-implantitis. METHOD AND MATERIALS: PubMed, Embase, and the Cochrane Library were searched for randomized controlled trials published from inception to January 2023. RESULTS: A total of five clinical trials with a total of 211 patients were included in the analyses. No significant difference was found in the reduction of probing pocket depth at 3 and 6 months of follow-up (3 months: weighted mean difference [WMD] = -0.336, 95% CI -0.966 to 0.233, P = .231; 6 months: WMD = -0.533, 95% CI -1.654 to 0.587, P = .351). A statistically significant difference was found at 12 months of follow-up (WMD = -1.327, 95% CI -1.803 to -0.852, P < .001) between the treatment and control groups. The combined results indicated that the differences in reduction of bleeding on probing, Plaque Index score, and bone level at 6 months of follow-up were significant (P < .05). CONCLUSION: The study demonstrated that the adjunctive use of systemic metronidazole and amoxicillin did not significantly improve probing pocket depth compared to nonsurgical treatment alone, and should not be routinely recommended. However, the significant reductions in bleeding on probing, Plaque Index, and bone level at 6 months may indicate a potential effect of treating peri-implantitis with adjunctive systemic metronidazole and amoxicillin.

Comparison of masticatory myofunctional rehabilitation combined with conventional functional appliances for the treatment of class â ¡, division 1 malocclusion. A retrospective cohort study.

OBJECTIVE: To estimate the clinical efficacy of early masticatory myofunction rehabilitation combined with conventional functional appliances for the treatment of class Ⅱ, division 1 malocclusion in orthodontic children during the growing phase. MATERIALS AND METHOD: A comparative retrospective cohort study, enrolled patients diagnosed with class Ⅱ/1 in the stage of late mixed or early permanent dentition. Patients were divided into a TBA group (Cohort 1): receiving Twin-block appliance treatment; and a MMR group (Cohort 2): receiving either early masticatory myofunction rehabilitation as adjunctive therapy combined with the same conventional functional appliances. The study variables were active (Phase 1) treatment duration, oral esthetic subjective impact score (OASIS), several cephalometric indices calculated from X-ray photographs, the maximum voltage (mV) and asymmetry index (AsI) of anterior temporalis (TA) and masseter muscles (MM) before and after treatment. Complications were also recorded. RESULTS: A total of 424 cases were enrolled. The mean treatment duration in the MMR group was 168.33 days (SD: 25.43) and 215.00 days (SD: 28.81) in the TBA group; mean difference: -46.67 days (95% CI: [-81.62, -11.71]), P<0.001. For the OASIS outcome measure, there was no statistically significant difference between the mean total scores for the MMR group (18.83±7.73) or TBA group (17.67±6.47) groups adjusted to include pre-treatment OASIS scores (P = 0.783). After treatment, sella-nasion-B point (SNB), mandibular incisor angle, maxillary base and mandibular base in both two groups were significantly increased, while AB plane angle (ANB), maxillary incisor angle, overjet and overbite were significantly decreased. The mV and AsI of TA and MM were also improved following treatment. However, no significant differences were observed between two groups. CONCLUSION: Our results confirmed that early masticatory myofunction rehabilitation in combination with conventional TBA for patients in the growing phase was significantly effective in the management of class Ⅱ/1 in orthodontic treatment, which could significantly shorten the treatment duration and had the similar improvement in the cephalometry data, OASIS scores and masticatory muscles function when comparing to conventional TBA alone.

Antibacterial and anti-inflammatory effects of PGLa-loaded TiO2 nanotube arrays.

Objectives: This study investigated the antimicrobial effect and anti-inflammatory activities of PGLa-loaded TiO2 nanotube arrays (TiO2 NTs) in osteoblast-like MG-63 cells. Methods: The surface morphology and roughness of three titanium (Ti) substrates (Ti, TiO2 NTs, PGLa-loaded TiO2 NTs) were evaluated by scanning electron microscopy (SEM) and atomic force microscope (AFM). The wettability of three titanium substrates was evaluated by contact angle. Biocompatibility of PGLa-loaded TiO2 NTs were evaluated in MG-63 cells (cell adhesion, proliferation, cytoskeletal evaluation and alkaline phosphatase activity). Spread plate counting method was used to evaluate antibacterial abilities of the titanium substrates. The calcein AM/PI staining evaluated cell viability of MG-63 cells on the substrates with or without proinflammatory factors (TNF-α). Results: The average surface roughness of untreated Ti, TiO2 NTs, PGLa-loaded TiO2 NTs were found to be 135.8 ± 6.4 nm, 300.5 ± 10.5 nm, 348.9 ± 16.9 nm, respectively. The contact angle of the untreated Ti was 77.4° ± 6.6°. TiO2 NTs displayed excellent wettability which of contact angle was 12.1° ± 2.9°. The contact angle of the PGLa-loaded TiO2 NTs was 34.6° ± 4.9°. MG-63 cells on surface of PGLa-loaded TiO2 NTs showed better cell adhesion, proliferation and osteogenic activity. The antibacterial rate of PGLa-loaded TiO2 NTs group significantly increased (84.6% ± 5.5%, p < 0.05). The rate of dead cells on the surfaces of the PGLa-loaded TiO2 NTs with TNF-α decreased significantly (4.49% ± 0.02, p < 0.01). Conclusion: PGLa-loaded TiO2 NTs have multi-biofunctions including biocompatibility, antibacterial and anti-inflammatory properties.