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1.
J Virol ; 96(16): e0042122, 2022 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-35924924

RESUMO

Weeds surrounding crops may act as alternative hosts, playing important epidemiological roles as virus reservoirs and impacting virus evolution. We used high-throughput sequencing to identify viruses in Spanish melon crops and plants belonging to three pluriannual weed species, Ecballium elaterium, Malva sylvestris, and Solanum nigrum, sampled at the edges of the crops. Melon and E. elaterium, both belonging to the family Cucurbitaceae, shared three virus species, whereas there was no virus species overlap between melon and the other two weeds. The diversity of cucurbit aphid-borne yellows virus (CABYV) and tomato leaf curl New Delhi virus (ToLCNDV), both in melon and E. elaterium, was further studied by amplicon sequencing. Phylogenetic and population genetics analyses showed that the CABYV population was structured by the host, identifying three sites in the CABYV RNA-dependent RNA polymerase under positive selection, perhaps reflecting host adaptation. The ToLCNDV population was much less diverse than the CABYV one, likely as a consequence of the relatively recent introduction of ToLCNDV in Spain. In spite of its low diversity, we identified geographical but no host differentiation for ToLCNDV. Potential virus migration fluxes between E. elaterium and melon plants were also analyzed. For CABYV, no evidence of migration between the populations of the two hosts was found, whereas important fluxes were identified between geographically distant subpopulations for each host. For ToLCNDV, in contrast, evidence of migration from melon to E. elaterium was found, but not the other way around. IMPORTANCE It has been reported that about half of the emerging diseases affecting plants are caused by viruses. Alternative hosts often play critical roles in virus emergence as virus reservoirs, bridging host species that are otherwise unconnected and/or favoring virus diversification. In spite of this, the viromes of potential alternative hosts remain largely unexplored. In the case of crops, pluriannual weeds at the crop edges may play these roles. Here, we took advantage of the power of high-throughput sequencing to characterize the viromes of three weed species frequently found at the edges of melon crops. We identified three viruses shared by melon and the cucurbit weed, with two of them being epidemiologically relevant for melon crops. Further genetic analyses showed that these two viruses had contrasting patterns of diversification and migration, providing an interesting example on the role that weeds may play in the ecology and evolution of viruses affecting crops.


Assuntos
Begomovirus , Produtos Agrícolas , Cucurbitaceae , Interações entre Hospedeiro e Microrganismos , Luteoviridae , Doenças das Plantas , Plantas Daninhas , Animais , Afídeos/virologia , Begomovirus/classificação , Begomovirus/genética , Produtos Agrícolas/virologia , Cucurbitaceae/virologia , Genética Populacional , Interações entre Hospedeiro e Microrganismos/genética , Luteoviridae/genética , Malva/virologia , Filogenia , Doenças das Plantas/virologia , Plantas Daninhas/virologia , RNA Polimerase Dependente de RNA/metabolismo , Solanum nigrum/virologia
2.
Arch Virol ; 168(1): 16, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36593371

RESUMO

We determined the complete genome sequence of a new virus infecting Ecballium elaterium ('cohombrillo amargo') plants, a weed species common on the borders of cultivated fields in the Mediterranean region. The genome of this virus is composed of two molecules of monocistronic positive-sense RNA, 6,934 and 3,501 nucleotides in length, excluding their poly(A) tails. The highest amino acid sequence similarity (50 % identity) in the Pro-Pol core region encoded by RNA 1 was observed in the corresponding protein of strawberry latent ringspot virus. Based on pairwise comparisons and phylogenetic analysis, this virus, tentatively named "cohombrillo-associated virus" (CoAV), appears to be a member of a new species in the genus Stralarivirus (family Secoviridae), for which the name "Stralarivirus elaterii" is proposed. This new virus has different putative cleavage patterns from members of other species belonging to this genus.


Assuntos
Vírus de Plantas , Secoviridae , Vírus Satélites/genética , RNA Viral/genética , Filogenia , Vírus de Plantas/genética , Genoma Viral , Doenças das Plantas , Fases de Leitura Aberta
3.
Virus Genes ; 59(1): 163-166, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36306006

RESUMO

In Brazil, the main viral disease of melon plant is severe yellowing disease called "Amarelão do Meloeiro," and a polerovirus, cucurbit aphid-borne yellows virus (CABYV) was considered one of the etiological agents. This virus is a recombinant strain originated from CABYV and unknown polerovirus. Due to unsuccessful mechanical inoculations of CABYV to host plants, the study of its biological characterization is hampered. Therefore, an infectious clone of the recombinant strain of CABYV was constructed using the Gibson Assembly technology. The full-length cDNA clones produced in this study showed to be infectious in three cucurbit species; melon (Cucumis melo), squash (a hybrid of Cucurbita maxima × C. moschata), and West Indian gherkin (Cucumis anguria) plants, but not in watermelon, cucumber, and zucchini plants. This insusceptibility of watermelon plants to the infectious clone corroborates the observation that this virus was never found in watermelon plants often located next to the infected melon plants. This infectious clone provides important tools for future study in developing resistant melon variety to CABYV infection.


Assuntos
Cucurbita , Cucurbitaceae , Luteoviridae , DNA Complementar/genética , Brasil , Luteoviridae/genética , Cucurbitaceae/genética , Cucurbita/genética , Plantas
4.
Virus Genes ; 58(4): 372-375, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35471489

RESUMO

In this work, a novel viral genomic sequence with a gene organization typical of members of the genus Soymovirus was identified using high-throughput sequencing data from common mallow. This species is a vigorous wild weed native to the Mediterranean region, commonly found in borders and edges of cultivated fields, making it a suitable reservoir for plant pests and pathogens. Indeed, plant viruses belonging to different genera have been previously found infecting common malva. This new viral genome consists of a single molecule of circular double-stranded DNA of 8391 base pairs and contains eight open reading frames encoding polymerase, movement, coat, translational transactivator protein typical of caulimoviruses, and four hypothetical proteins. Phylogenetic and pairwise distance analyses showed its close relationship with soybean chlorotic mottle virus. Interestingly, a small intergenic region was detected between ORFs Ib and II. Based on the demarcation criteria of the genus Soymovirus, the new virus, provisionally named malva-associated soymovirus 1, could be a member of a new species Soymovirus masolus. To our knowledge, this is the first report of a soymovirus infecting common mallow.


Assuntos
Caulimoviridae , Malva , Caulimoviridae/genética , Genoma Viral/genética , Sequenciamento de Nucleotídeos em Larga Escala , Fases de Leitura Aberta , Filogenia , Doenças das Plantas
5.
Virus Genes ; 56(6): 792-795, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33026576

RESUMO

In this work, a novel ssRNA (+) viral genomic sequence with gene organization typical of members of the subfamily Quinvirinae (family Betaflexiviridae) was identified using high- throughput sequencing data of date palm obtained from the Sequence Read Archive database. The viral genome sequence consists of 7860 nucleotides and contains five ORFs encoding for the replication protein (Rep), triple gene block proteins 1, 2, 3 (TGB 1, 2, and 3), and coat protein (CP). Phylogenetic analysis based on the Rep and the CP amino acid sequences showed the closest relationship to garlic yellow mosaic-associated virus (GYMaV). Based on the demarcation criteria of the family Betaflexiviridae, this new virus, provisionally named date palm virus A (DPVA), could constitute a member of a novel genus. However, considering that DPVA and GYMaV share the same genomic organization and that they cluster together on the Rep phylogenetic analysis, they could also constitute a novel genus together, highlighting the necessity of a revision of the taxonomic criteria of the family Betaflexiviridae.


Assuntos
Flexiviridae , Genoma Viral , Phoeniceae/virologia , Doenças das Plantas/virologia , RNA Viral , Flexiviridae/classificação , Flexiviridae/genética , Flexiviridae/isolamento & purificação , Filogenia
6.
Front Microbiol ; 14: 1181562, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37323908

RESUMO

The advances in high-throughput sequencing (HTS) technologies and bioinformatic tools have provided new opportunities for virus and viroid discovery and diagnostics. Hence, new sequences of viral origin are being discovered and published at a previously unseen rate. Therefore, a collective effort was undertaken to write and propose a framework for prioritizing the biological characterization steps needed after discovering a new plant virus to evaluate its impact at different levels. Even though the proposed approach was widely used, a revision of these guidelines was prepared to consider virus discovery and characterization trends and integrate novel approaches and tools recently published or under development. This updated framework is more adapted to the current rate of virus discovery and provides an improved prioritization for filling knowledge and data gaps. It consists of four distinct steps adapted to include a multi-stakeholder feedback loop. Key improvements include better prioritization and organization of the various steps, earlier data sharing among researchers and involved stakeholders, public database screening, and exploitation of genomic information to predict biological properties.

7.
Microorganisms ; 9(5)2021 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-34066188

RESUMO

We used high-throughput sequencing to identify viruses on tomato samples showing virus-like symptoms. Samples were collected from crops in the Iberian Peninsula. Either total RNA or double-stranded RNA (dsRNA) were used as starting material to build the cDNA libraries. In total, seven virus species were identified, with pepino mosaic virus being the most abundant one. The dsRNA input provided better coverage and read depth but missed one virus species compared with the total RNA input. By performing in silico analyses, we determined a minimum sequencing depth per sample of 0.2 and 1.5 million reads for dsRNA and rRNA-depleted total RNA inputs, respectively, to detect even the less abundant viruses. Primers and TaqMan probes targeting conserved regions in the viral genomes were designed and/or used for virus detection; all viruses were detected by qRT-PCR/RT-PCR in individual samples, with all except one sample showing mixed infections. Three virus species (Olive latent virus 1, Lettuce ring necrosis virus and Tomato fruit blotch virus) are herein reported for the first time in tomato crops in Spain.

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