An ATP responsive fluorescent supramolecular assembly based on a polyelectrolyte and an AIE active tetraphenylethylene derivative.

Aggregation induced emission (AIE) active probes have attracted enormous attention due to their wide-spread and ever increasing number of applications in the sensing of chemically and biologically important molecules. AIE probes undergo drastic modulation in their photophysical features from a monomeric to aggregated state. In the current work, we report the aggregation of tetra-anionic Su-TPE (AIE active probe) in the presence of a cationic polyelectrolyte, poly(allylaminehydrochloride) (PAH). A supramolecular assembly is formed by the electrostatic interaction between cationic PAH and anionic Su-TPE molecules, which leads to drastic modulations in the spectral features of anionic Su-TPE upon addition of cationic PAH. The Su-TPE-PAH aggregate assembly has been investigated using various photophysical techniques, such as, ground-state absorption, steady-state and time-resolved emission spectroscopic techniques along with 1H NMR measurements. The Su-TPE-PAH aggregate assembly is found to be responsive towards the ionic strength of the medium and temperature which results in drastic modulations of the spectral features of the emissive supramolecular aggregate assembly. Finally, the specific recognition of an important bioanalyte, ATP, has been achieved using the formed Su-TPE-PAH supramolecular aggregate assembly as a sensing platform which displays good selectivity and high sensitivity towards ATP. Importantly, the developed sensor platform could also function in the human serum matrix, hence, demonstrating the potential of the established sensor platform for real-life applications in near future.

Patented AIE materials for biomedical applications.

In recent years aggregation induced emission (AIE) concept has attracted researcher's interest worldwide. Several organic building blocks are developed as AIE materials. This chapter discusses the patented AIE material and their utilization related in biological, medicinal and biotechnology fields. It is demonstrated that AIE chromophores such as tetraphenylethylene (TPE) as well as other AIE building blocks became important fluorescent emissive bioactive materials. Such emissive materials are widely employed as bioprobes for the detection of mitochondria, cellular imaging and tracking, protein carrier detection of S-phase DNA, detection of d-glucose, visualization of cancer treatment, drug screening, image-guided therapy, bacterial imaging, photodynamic therapy and drug screening. Such AIE materials upon imaging in cellular environment displays significant enhancement of fluorescence emission. Such patented AIE chromophores has a great potential for bioimaging and biomedical applications. In this chapter we compile some patented representative examples to explore their bioimaging/medicinal imaging applications since lot of new inventions are reported every day.

Host-Assisted Aggregation-Induced Emission of a Tetraphenylethylene Derivative and Its Responses toward External Stimuli.

Aggregation-induced emission (AIE) of fluorogenic dyes offers many opportunities as smart materials, fluorescence sensing of analytes, bioimaging, molecular electronics, and many others. AIE dyes (called AIEgens) produce emission through aggregation, which are more advantageous than conventional emission of monomeric fluorophores, as the latter is unduly susceptible toward various quenching processes. Here, we report AIE enhancement of a polyanionic sulfonato-tetraphenylethylene (SuTPE) derivative, achieved through supramolecularly assisted dye aggregation, as SuTPE interacts with a multicationic amino-ß-cyclodextrin (AßCD) host. Aggregation of the dye is induced mainly because of strong electrostatic interaction of SuTPE with AßCD, causing a significant extent of charge neutralization for the polyanionic dyes, helping their assemblage at the multicationic host portal. Job's plot studies suggest preferential formation of 2:1 dye-to-host stoichiometric complexes in the present system. Ionic-strength-dependent studies nicely support the involvement of electrostatic interaction in the present system through salt-induced disintegration of the SuTPE-AßCD complexes. The AIE enhancement for the SuTPE-AßCD system is very sensitive to the external stimuli, such as pH and temperature, suggesting its prospects in various stimuli-responsive applications. Furthermore, the SuTPE-AßCD system can suitably quantify an important bioanalyte, ATP, following a competitive binding strategy, suggesting its potential application as a supramolecular biosensor.