Bycatch and pollution are the main threats for Burmeister's porpoises inhabiting a high-industrialized bay in the Humboldt Current System.

Pollution and bycatch are two of the main threats for cetaceans worldwide. These threats are exacerbated for nearshore species particularly for those in regions with intense industrial and fishing activities. Burmeister's porpoise is endemic to South America, has a Near Threatened conservation status because of long-term mortality in fisheries. Burmeister's porpoise occur in Mejillones Bay, northern Chile, a hot spot for heavy metals pollution from the mining industry and an intense industrial and artisanal purse-seine fishing area. From 2018 to 2021, we conducted systematic marine surveys to assess the abundance, distribution and habitat use of Burmeister's porpoises. We responded to stranding reports from 2018 to 2022, and necropsied nine individuals. From five of these, we analyzed the metal concentrations (As, Cd, Cr, Cu, Pb, Hg, Se and Zn) in muscle and skin tissues. Results showed an abundance of 76.17 individuals (CV = 25.9%) and an average density of 0.45 individuals/km2 (CV = 26%). Burmeister's porpoises were observed year round, 22.2% were mother-calf pairs present in austral summer at an average of 90.6 m depth in the southwestern bound of the bay. Two-thirds of stranded specimens died due to bycatch and one died due to bottlenose dolphin (Tursiops truncatus) attack. We report a dead Burmeister's porpoise positive for avian flu virus A (H5N1). Metals analyzed were found in muscle and skin tissues of stranded Burmeister's porpoises in the following order (Zn > Cu > Cr > As > Hg > Pb > Cd). Although we could not assess pollution as a cause of mortality, Cr, As and Pb concentrations exceeded the concentrations found in other porpoises species worldwide. We conclude that bycatch and pollution as the main threats for Burmeister's porpoise survival in northern Chile. Future studies should investigate the use of acoustic deterrent alarms to mitigate the bycatch in the bay and consider the Burmeister's porpoise as a sentinel species of pollution in northern Chilean coast.

Diversity of compounds in Vespa spp. venom and the epidemiology of its sting: a global appraisal.

Poisonous animals imply a risk to human life, because their venom is a complex mixture of low molecular weight components, peptides and proteins. Hornets use the venom for self-defence, to repel intruders and to capture prey, but they can cause poisoning and allergic reactions to people. In particular, they seem to be a health problem in the countries where they are native due to their sting, which in the most severe cases can lead to severe or fatal systemic anaphylaxis. But this situation is being an emerging problem for new countries and continents because hornet incursions are increasing in the global change scenario, such as in Europe and America. Furthermore, 55 detailed cases of hornet sting were found in 27 papers during the current review where 36.4% died due to, mainly, a multi-organ failure, where renal failure and liver dysfunction were the most common complications. Moreover, the great taxonomic, ecological diversity, geographical distribution and the wide spectrum of pathophysiological symptoms of hornets have been the focus of new research. Considering this, the present systematic review summarizes the current knowledge about the components of Vespa venom and the epidemiology of its sting to serve as reference for the new research focused on the development of techniques for diagnosis, new drugs and treatments of its sting.

Mercury and Organochlorine Pesticides in Tissues of Loggerhead Sea Turtles (Caretta caretta) Stranded Along the Southwestern Mediterranean Coastline (Andalusia, Spain).

Nineteen loggerhead sea turtles (Caretta caretta) stranded along the southwestern Mediterranean coastline (Andalusia) were used in this study. A total of 68 samples of fat (n = 18), liver (n = 15), kidney (n = 13), pectoral muscle (n = 19), and brain (n = 3) were analysed for total mercury (Hg) and organochlorine pesticides [OC: ∑Dichlorodiphenyltrichloroethanes (∑DDT), ∑Hexachlorocyclohexane (∑HCH), ∑Heptachlor, ∑Drins and ∑Endosulfan]. These loggerhead sea turtles showed tissue Hg and OC concentrations similar to or lower than those reported in other studies. Few growth-related variations in Hg or OC levels in relation to straight carapace length were found, probably because the specimens were mostly juveniles. This study will help to fill the gap on spatio-temporal exposure data and ascertain the real world-wide picture of the contamination levels in loggerhead sea turtles.

Spatiotemporal variations of organochlorine pesticides in an apex predator: Influence of government regulations and farming practices.

BACKGROUND: Intensification of agricultural practices has caused several negative effects to the environment. The use of fertilizers and pesticides may alter geochemical cycles or cause direct wildlife intoxication. Detrimental effects of organochlorine pesticides (OCPs) have forced the authorities to ban or restrict its use. This study evaluates the variation in levels of OCPs in a sentinel species in relation to changes in government regulations and the spatial configuration of agricultural practices around the nests. METHODS: Between 2003 and 2007, we analysed OCP levels in 256 blood samples of Eurasian Eagle-owl (Bubo bubo) chicks nesting in area of intensive commercial agriculture with historical frequent use of pesticides, in South-eastern Spain. We studied year-to-year variations in OCP concentrations and their relation with land use configuration around raptor nests by Generalized Linear Mixed Models (GLMM). RESULTS: OCPs were detected in 100% samples surveyed in 2003 and 2004, while dropped to 27% in 2005, 6.8% in 2006 and 6.3% in 2007, coinciding with the ban of OCPs. The presence of the main OCPs was related to agricultural practices. In particular, endosulfan and lindane were related to irrigated crops and urban areas, while DDT-related compounds and dieldrin were associated with dry land farming. CONCLUSIONS: OCP concentrations in blood samples of Eurasian Eagle-owls may respond quickly to the implementations of new regulations about the use of agricultural products. This raptor was confirmed as a good sentinel species allowing rapid detection of changes in pesticides use.

Interspecific differences in the antioxidant capacity of two Laridae species exposed to metals.

The main aim of the present study was to assess the concentration of metals (Pb, Cd, Hg, Cu and Zn) in blood, and elucidate their potential effects on oxidative stress biomarkers in red blood cells of Audouin's gull (Ichthyaetus audouinii) and Slender-billed gull (Chroicocephalus genei) chicks in Southeastern Spain. For this purpose, total glutathione (GSH) content, antioxidant enzymes activities (glutathione peroxidase, GPx; superoxide dismutase, SOD; catalase, CAT and glutathione-S-transferase, GST), and lipid peroxidation (TBARS) were analyzed. In general, metal exposure in both species can be considered low, except for Hg in Audouin's gull. Our findings show higher antioxidant levels in Audouin's gull than in Slender-billed gull; probably due to a combination of different basal antioxidant capacity between species, and to an up-regulation of the antioxidant system in Audouin's gull as a response to the higher Hg, Cu and Zn concentrations. This could reduce the production of TBARS keeping them at lower levels than those found in Slender-billed gull. In spite of this, the significantly higher Hg levels found in Audouin's gull (13.6µg/dl wet weight) in comparison to Slender-billed gull (2.7µg/dl), likely related to their different diet and the former consuming discarded fish, were able to produce lipid peroxidation in this species. The positive effect of Hg on SOD activity in Slender-billed gull, and of Pb on GSH levels in Audouin's gull, could reflect the necessity of the organism to upregulate these antioxidants to balance the increased oxidative stress caused by metals. The degree of metal exposure seems to be essential in the response of the antioxidant system, which may suffer up or down-regulations depending on metal concentrations. This study supports the interactive effects of metals on oxidative stress biomarkers, the complexity of the antioxidant system and the close cooperation between antioxidants, which requires the study of several metals and biomarkers to evaluate oxidative stress and damage in wild birds.

Delta-aminolevulinic acid dehydratase (δALAD) activity in four free-living bird species exposed to different levels of lead under natural conditions.

The purposes of this study were: (1) to determine the δALAD activity and δALAD ratio in blood of four free-living bird species (Griffon vulture, Eagle owl, Slender-billed gull and Audouin's gull); (2) and to investigate the correlations between δALAD activity/ratio and Pb concentrations in blood samples. A decrease was observed in δALAD activity in Griffon vultures and Eagle owls exposed to Pb. In addition, negative relationships were found between δALAD ratio or δALAD activity and Log blood Pb levels in Griffon vultures and Eagle owls, and these relationships were stronger in areas with the highest Pb exposure. We provide equations that may be helpful to estimate δALAD activity and δALAD ratio using blood Pb concentrations. Regarding gull species, δALAD activity found in the present study may be considered the normal activity in Slender-billed gull and Audouin's gull species, since very low blood Pb concentrations and no correlations were found in these species. Although both δALAD activity and δALAD ratio are sensitive biomarkers of Pb exposure and effect in birds, the use of δALAD ratio may improve the results. Besides, this study provides blood threshold concentrations at which Pb bears effects on δALAD enzyme (5µg/dl in Eagle owl; 8µg/dl in Griffon vulture; and probably >2µg/dl in Slender-billed gull and Audouin's gull). Our findings show that Eagle owl seems to be more sensitive to δALAD enzymatic inhibition by Pb than Griffon vultures. Eagle owls and Griffon vultures exhibited up to 79% and 94% decrease in δALAD activity when blood Pb concentrations exceeded 19 and 30µg/dl, respectively. Regarding the effects related with δALAD inhibition, significant negative correlations were found between δALAD activity and hematocrit in Eagle owls and Griffon vultures, which may be related to compensatory response associated with a decrease in δALAD activity. In addition, an effect on creatine kinase activity and total proteins in plasma was found in Griffon vultures. The significant negative correlations found between δALAD activity and tGSH in Griffon vulture, and between δALAD activity and Log CAT activity in Eagle owls, may be related to a protective response of antioxidant system against reactive oxygen species (ROS). The negative relationship found between δALAD activity and TBARS levels in Griffon vulture is probably related to an induction of lipid peroxidation by ROS that may be generated by δALA accumulation when δALAD activity is depressed. δALAD activity and δALAD ratio in blood are suggested as important nondestructive biomarkers for Pb exposure and effect for future biomonitoring studies in Griffon vulture and Eagle owl. Further studies are recommended to provide new data on Pb concentrations at which δALAD activity is affected in different wild bird species and to elucidate why different species tolerate Pb in different ways.

Effects of heavy metals on biomarkers for oxidative stress in Griffon vulture (Gyps fulvus).

Metals are involved in the formation of reactive oxygen species (ROS) which may result in metal-related oxidative stress that can lead to oxidative damage to lipids, DNA and proteins. It is necessary to understand the mechanisms of metal toxicity in wild birds, and the concentrations that cause effects on oxidative stress biomarkers. The aim of this study is to assess the concentrations of lead (Pb), cadmium (Cd), mercury (Hg), copper (Cu) and zinc (Zn) with regards to oxidative stress in blood samples of 66 Griffon vultures (Gyps fulvus) from two areas of the Autonomous Community of Valencia (East of Spain). The two study areas (Alcoy n=36 and Cinctorres n=30) were selected as random locations of interest that had not yet been studied, and are feeding stations where supplementary food, mainly of pork origin, is provided for vultures. Given that the two study areas are not considered polluted sites, we expected to find low metal concentrations. However, there are no known threshold concentrations at which metals can affect antioxidant systems, and low metal levels may have an effect on antioxidant biomolecules. In this study, since sampling was done at the beginning of the hunting season, the low Pb levels found in most Griffon vultures from Alcoy and Cinctorres (median=12.37 and 16.26µg/dl, respectively) are suggestive of background levels usually found in vultures that feed on pork carcasses all year round. The ingestion of game meat with bullet fragments in carcasses or with Pb shots embedded in the flesh could be the cause of the high blood Pb concentrations found in three vultures from Cinctorres (83, 290 and 362µg/dl). Griffon vultures feeding in Cinctorres had enhanced CAT and GST activities and tGSH concentrations, which may be interpreted as protective response against the higher TBARS levels. This study provides threshold concentrations at which metals affect antioxidant system derived from 66 samples of Griffon vulture. Blood Cd concentrations greater than 0.05µg/dl produced an induction of 33% in GPx and of 44% in CAT activity in erythrocytes of vultures from Alcoy. Hg concentrations in blood higher than 3µg/dl produced an induction of 10% in SOD activity. Concentrations of Pb above 15µg/dl in blood produced an inhibition of 12.5% in GPx and 11.3% in CAT activity, and a TBARS induction of 10.7% in erythrocytes of Griffon vultures.

Oxidative stress biomarkers in Eurasian eagle owls (Bubo bubo) in three different scenarios of heavy metal exposure.

The main aim of the present study is the assessment of oxidative stress related to metals in the Eurasian eagle owl (Bubo bubo) from three areas (agricultural and rural area, industrial area, and mining area) of Murcia, Southern Spain. Mean blood metal concentrations were Cd=0.07±0.21, Pb=3.27±5.21, Cu=10.62±4.77, Zn=311.47±67.14, Hg=2.32±3.83 µg/dl wet weight. Although individuals from the mining area had significant higher Pb and Hg concentrations, and significant lower glutathione-S-transferase (GST) and catalase (CAT) activities in red blood cells (RBC); the lack of differences in oxidative damage to membrane lipids (TBARS) among areas suggests that the antioxidant capacity of the different populations is able to deal with oxidant species and maintain TBARS levels in the same amount. Despite the low levels of metals, several oxidative stress biomarkers were correlated with metal concentrations. This study provides threshold concentrations at which metals cause effects on the antioxidant system in Eagle owls. Blood Cd concentrations greater than 0.3 µg/dl produced an inhibition in GPx (32%) and CAT (26%) activity in RBC. However, Cd concentrations higher than 0.02 µg/dl were enough to produce an inhibition of these enzymes. Regarding Pb levels, blood concentrations above 2 µg/dl produced an inhibition of 8% and 10.5% in GPx and CAT activities, respectively, in RBC. A depletion of 16% and 4% in tGSH levels was associated with Pb concentrations higher than 15 and 3 µg/dl, respectively, in individuals from the ancient mine site. In addition, Pb concentrations above 2 and 10 µg/dl produced a TBARS induction of 10% and 28%, respectively, in individuals from both the industrial and the mining area. Finally, Hg concentrations greater than 3 and 10 µg/dl resulted in a TBARS induction of 102% and 190%, respectively, in Eurasian eagle owls from the industrial area. Our findings show that Pb may produce effects on oxidative stress biomarkers in Strigiformes at lower concentrations than those typically accepted for considering physiological effects in Falconiformes (20 µg/dl in blood). In addition, we provide new data on Hg and Cd concentrations related to effects in the antioxidant system.

Feathers as a biomonitoring tool of polyhalogenated compounds: a review.

Feathers have many advantages that make them an excellent nondestructive tool for monitoring polyhalogenated compounds (PHCs). This paper proposes a review on the PHCs in feathers and factors influencing the pollutant load. Special attention has given to external contamination and the main analytical methods used to detect these compounds in feathers. Some authors have found strong and significant correlations between the concentrations of PHCs in feathers and internal tissues, providing positive expectations for their future use in the field of ecotoxicology. However, changes in diet, time elapsed between the previous molt period and sampling, sample size, and/or external contamination have been suggested as possible causes to explain the lack of correlations reported in some studies. Further studies with newly grown feathers and blood samples would be required in order to clarify this issue. Although atmospheric deposition has been reported as cause of external contamination, preening oil seems to be the most relevant factor contributing to this process. Unfortunately, washing techniques tested to date are not able to effectively remove the surface contamination from barbs and shafts, and therefore, it is necessary to develop methods able to discriminate between internal and external contamination. Finally, in this review, deposition rate is proposed as a measurement unit, as this allows comparisons between different parts of the same feather, as well as between different feathers.

Metal concentrations in feathers of red-legged cormorants (Phalacrocorax gaimardi) and sources of plastic in a nesting colony from northern Chile.

Mining industry is increasing the concentrations of heavy metals in coastal ecosystems of northern Chile. The presence of plastic in the nests of red-legged cormorants could be acting as a vector of metal presence in their organism. Here we analyze the source of plastic in the nests of cormorants by characterizing the abundance and type of marine debris in beaches and sea surface and the concentrations of eight metals in their feathers. Plastic was present in overall sampled nests and floating and beach debris are not the main sources of plastic to the nests. Overall analyzed metals were present in the cormorants in the following order: Zn > Cu > Se > Pb > Cd > As > Hg > Cr and Se, Cd and Pb concentrations were above the toxic limit. Further studies should investigate the potential adverse effects that plastics and metals presence may cause in this nesting colony.

Behavioural response to toxic elements, detoxification and organ accumulation are time-of-day-dependent in zebrafish.

Toxic elements, such as mercury (Hg) and arsenic (As), are major pollutants in aquatic environments, posing ecological threats to living organisms due to their toxicity and bioaccumulation. This paper investigated whether zebrafish response to Hg and As displayed day/night differences. Fish were exposed to either 35 µg/L of mercury chloride for 6 h or 65 mg/L of sodium arsenate for 4 h, at two different times of the day: mid-light (day; ML) and mid-darkness (night; MD). Fish were video-recorded to investigate their behavioural response and at the end of each trial, gills and liver samples were collected for gene expression measurement. Gills, liver and brain samples were also obtained to determine Hg and As concentration. A control group (non-exposed) was video-recorded and sampled too. The effect of Hg and As on zebrafish swimming activity and the expression of antioxidant and metallothionein genes was time-of-day-dependent, with a stronger response being observed during the day than at night. However, the neurobehavioural effect of Hg was more affected by the time of exposure than the effect of As. In addition, Hg concentration in the gills was significantly higher in zebrafish exposed at ML than at MD. Altogether, these findings suggest that zebrafish response to Hg and As is time-of-day-dependent and remark the importance of considering toxicity rhythms when using this fish species as a model in toxicological research.

Razorbill (Alca torda) feathers as an alternative tool for evaluating exposure to organochlorine pesticides.

The aim of this study was to explore the usefulness of feathers as a biomonitoring tool for organochlorine pesticides (OC) in a razorbill population (Alca torda). Fifteen OC were analyzed in feathers, including α-, ß- and δ-hexachlorocyclohexane, lindane, aldrin, dieldrin, endrin, endosulfan I and II, endosulfan sulfate, p,p'-DDT, DDD, DDE, heptachlor and its epoxide. The geometric mean concentrations observed in this study were ∑DDT 67.40 ng/g, ∑HCH 62.88 ng/g, ∑Heptachlor 61.75 ng/g, ∑Endosulfan 19.70 ng/g, and ∑Drins 10.17 ng/g. The higher OC levels found in this study compared with other studies are probably affected by the razorbill diet and migration status. However, levels found in the feathers of the present study are related to concentrations in internal tissues below those which cause adverse reproductive and behavioral effects or other signs of organochlorine-pesticide poisoning in birds. Age does affect the concentration of OC pesticides in feathers. Thus, feathers would appear to be a promising tool for OC biomonitoring in seabirds, since it is possible to quantify OC compounds.

A comparison of BGM and LLC-PK1 cells for the evaluation of nephrotoxicity.

Nephrotoxicity is one of the most frequent effects observed after the use of medicine. Such situations have been tardily discovered because of existing methods to determine toxicity. The validation of sensitive, alternative methods for the early identification of toxic effects is as important as restrictions on the use of animals. In this light, the present study evaluated the effects of gentamicin on BGM and LLC-PK1 cells, using MTT and Neutral Red (NR). Although the LLC-PK1 cell line is used for toxicological studies, the BGM cell line is relatively new for this purpose. MTT (BGM: EC(50) = 6.29 mM; LLC-PK1: EC(50) = 8.01 mM) was found to be more sensitive than NR (EC(50) was greater than 10 mM for both cells). By using MTT, both cells demonstrated the involvement of mitochondria in a manner that was dose dependent, with an apoptotic process occurring at the concentrations of 1 and 3 mM and necrosis at concentrations above 4 mM. It could, therefore, be concluded that 1) BGM appears to be useful in the study of the mechanism of nephrotoxicity caused by gentamicin and 2) because of its sensitivity to MTT, in addition to its ease of manipulation, it is believed that the BGM cell line can also be used as an alternative method to evaluate nephrotoxicity.

Enzymatic activity changes in striped catfish Pseudoplatystoma magdaleniatum, induced by exposure to different concentrations of ibuprofen and triclosan.

The present study aimed to evaluate the effects of exposure for four months, with ibuprofen and triclosan at 25 and 50 µg/L in Striped catfish Pseudoplatystoma magdaleniatum, evaluated between sexes and exposure times. Biochemical biomarkers such as lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyltransferase, acetylcholinesterase, creatine kinase, lipid peroxidation, albumin, globulins, creatinine, and urea were evaluated. The results of this study suggest that both ibuprofen and triclosan at concentrations of 25 and 50 µg/L can cause alterations to P. magdaleniatum, interfering with the activity of certain enzymes associated with energy production, immune response, architecture, and cellular physiology. Also, we determined the current state of contamination in fish, the concentration of ibuprofen and triclosan in P. magdaleniatum muscle samples from the different places markets located on the banks of the main rivers of Colombia was quantified by UHPLC-QqQ-MS/MS, in three climatic periods; finding triclosan levels in the dry season in some of the sampling points compatible with enzyme-level alterations in this species.

Updating the use of biochemical biomarkers in fish for the evaluation of alterations produced by pharmaceutical products.

The evaluation of toxic effects in stressful environmental conditions can be determined through the imbalance between exogenous factors (environmental contaminants) and enzymatic and non-enzymatic defenses in biological systems. The use of fish for the identification of alterations in biochemical biomarkers provides a comprehensive vision of the effects that pharmaceutical products cause in the aquatic ecosystem, as they are organisms with high sensitivity to contaminants, filtering capacity, and potential for environmental toxicology studies. A wide range of pharmaceuticals can stimulate or alter a variety of biochemical mechanisms, such as oxidative damage to membrane lipids, proteins, and changes in antioxidant enzymes. This review includes a summary of knowledge of the last 20 years, in the understanding of the different biochemical biomarkers generated by exposure to pharmaceuticals in fish, which include different categories of pharmaceutical products: NSAIDs, analgesics, antibiotics, anticonvulsants, antidepressants, hormones, lipid regulators and mixtures. This review serves as a tool in the design of studies for the evaluation of the effects of pharmaceutical products, taking into account the most useful biomarkers, type of matrix, enzyme alterations, all taking the pharmaceutical group of interest.

A schematic sampling protocol for contaminant monitoring in raptors.

Birds of prey, owls and falcons are widely used as sentinel species in raptor biomonitoring programmes. A major current challenge is to facilitate large-scale biomonitoring by coordinating contaminant monitoring activities and by building capacity across countries. This requires sharing, dissemination and adoption of best practices addressed by the Networking Programme Research and Monitoring for and with Raptors in Europe (EURAPMON) and now being advanced by the ongoing international COST Action European Raptor Biomonitoring Facility. The present perspective introduces a schematic sampling protocol for contaminant monitoring in raptors. We provide guidance on sample collection with a view to increasing sampling capacity across countries, ensuring appropriate quality of samples and facilitating harmonization of procedures to maximize the reliability, comparability and interoperability of data. The here presented protocol can be used by professionals and volunteers as a standard guide to ensure harmonised sampling methods for contaminant monitoring in raptors.

A review of constraints and solutions for collecting raptor samples and contextual data for a European Raptor Biomonitoring Facility.

The COST Action 'European Raptor Biomonitoring Facility' (ERBFacility) aims to develop pan-European raptor biomonitoring in support of better chemicals management in Europe, using raptors as sentinel species. This presents a significant challenge involving a range of constraints that must be identified and addressed. The aims of this study were to: (1) carry out a comprehensive review of the constraints that may limit the gathering in the field of raptor samples and contextual data, and assess their relative importance across Europe; and (2) identify and discuss possible solutions to the key constraints that were identified. We applied a participatory approach to identify constraints and to discuss feasible solutions. Thirty-one constraints were identified, which were divided into four categories: legal, methodological, spatial coverage, and skills constraints. To assess the importance of the constraints and their possible solutions, we collected information through scientific workshops and by distributing a questionnaire to stakeholders in all the countries involved in ERBFacility. We obtained 74 answers to the questionnaire, from 24 of the 39 COST participating countries. The most important constraints identified were related to the collection of complex contextual data about sources of contamination, and the low number of existing raptor population national/regional monitoring schemes and ecological studies that could provide raptor samples. Legal constraints, such as permits to allow the collection of invasive samples, and skills constraints, such as the lack of expertise to practice necropsies, were also highlighted. Here, we present solutions for all the constraints identified, thus suggesting the feasibility of establishing a long-term European Raptor Sampling Programme as a key element of the planned European Raptor Biomonitoring Facility.

Temporal changes in metal concentrations in Andean condor feathers: a potential influence of volcanic activity.

Geothermal activities (e.g., volcanic eruptions) represent one of the most important natural sources of metal emissions (heavy metals and metalloids). They can be one of the main risks for the ecosystems in regions like North of Argentiniean Patagonia, a sparsely populated area, close to an extensive network of active volcanoes on the Andes Range. The 2011 eruption of the Puyehue-Cordon Caulle volcanic complex (PCCVC) has been the largest volcanic event of the last decades. The effects of exposure to ashes on wildlife and humans have been sparsely studied, and only one biomonitoring study has used higher trophic species. The exposure to metals of the species in Patagonia has been poorly studied. The main objectives of our study were to assess metal screening and to evaluate a possible relation between the levels of metals in the Andean condor (Vultur gryphus) population and the volcanic activity of the area. We investigated the effects of the eruption of the PCCVC in 2011, using samples of molt primary feathers of the Andean condor, collected in nine roosts around Bariloche, Argentina (maximum distance 85 km). Data available suggest the molt of the primary feathers of the Andean condor has a duration of 6 years. We carried out sampling before (2007, 2009) and after (2017) the volcanic eruption (2011). The feathers sampled in 2017 should have been developed in 2011-2012, reflecting the environmental situation of the period immediately following the eruption of the PCCVC. For the first time, we have screened metals in 48 molted primary feathers of Andean condor, showing the levels of 9 metals and metalloids (Si, Cr, Cu, Zn, As, Se, Cd, Pb, Hg). Si, Zn, As, and Cd showed higher levels in the feathers sampled after the eruption. The levels of Cr and Pb (although apparently not related to the volcanic eruption) in some samples are compatible with potential adverse effects in living organisms. The screening results represent an important database (the first for this species) that can be used in in future studies for comparative purposes. HIGHLIGHTS: •We present the first database of metals in condor feathers, which may be useful for future studies. •Volcanic eruptions might represent an important source of metal and metalloid emissions in this area. •The Andean condor from Argentine Patagonia could be affected by volcanic activity in the area. •Andean condor feathers may be good biomonitoring units of this contamination. •Pb pollution does not seem to be related to the volcano's eruption.

Changes in blood pesticide levels in booted eagle (Hieraaetus pennatus) associated with agricultural land practices.

We estimated the degree of exposure to lindane and endosulfan in the blood of booted eagle nestlings (Hieraaetus pennatus) (1999-2003), in order to assess the usefulness of these samples as a unit for monitoring changes in exposure as a result of shifts in agricultural practices and the implementation of legal measures. The highest blood lindane concentrations were obtained 1 year prior to its prohibition by the European Union. Subsequent to that year, the drop in blood concentrations was dramatic. Furthermore, endosulfan blood concentrations follow a progression coinciding with an increase in olive, grape and plum-tree crops. We conclude that concentrations of organochlorine pesticides in the blood of booted eagle nestlings may be used to monitor the use of those pesticides over a particular agricultural region and alert the authorities of possible environmental or health risks.

Heavy metals in tissues from loggerhead turtles (Caretta caretta) from the southwestern Mediterranean (Spain).

Cadmium, lead, copper, and zinc were measured in tissues of 21 loggerhead turtles (Caretta caretta) from the southwestern Mediterranean coastline. Mean concentrations (dry weight) of essential elements (Zn and Cu) were 107 and 21.6 microg/g in liver, 27.9 and 3.8 microg/g in kidney, 65.4 and 5.0 microg/g in pectoral muscle, 11.1 and 3.45 microg/g in brain, and finally 19.2 microg/g and undetected in bone, respectively. Mean concentrations of heavy metals (Cd and Pb) were 23.4 and 2.8 microg/g in liver, 31.5 and 0.5 microg/g in kidney, 0.2 and 0.3 microg/g in pectoral muscle, 0.2 and 0.7 microg/g in brain, and undetected and 1.2 microg/g in bone, respectively. Metal concentrations were similar to other studies conducted on Mediterranean turtles. However, cadmium concentrations varied widely among individuals, which has been associated with potential sources of cadmium in Mediterranean Sea. This is the first study into metal accumulation in tissues of loggerhead turtle from Spanish Mediterranean coastline.