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J Appl Genet ; 63(2): 423-428, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35279801

RESUMO

Analysis of the SARS-CoV-2 transcriptome has revealed a background of low-frequency intra-host genetic changes with a strong bias towards transitions. A similar pattern is also observed when inter-host variability is considered. We and others have shown that the cellular RNA editing machinery based on ADAR and APOBEC host-deaminases could be involved in the onset of SARS-CoV-2 genetic variability. Our hypothesis is based both on similarities with other known forms of viral genome editing and on the excess of transition changes, which is difficult to explain with errors during viral replication. Zong et al. criticize our analysis on both conceptual and technical grounds. While ultimate proof of an involvement of host deaminases in viral RNA editing will depend on experimental validation, here, we address the criticism to suggest that viral RNA editing is the most reasonable explanation for the observed intra- and inter-host variability.


Assuntos
COVID-19 , Edição de RNA , Adenosina Desaminase/genética , Adenosina Desaminase/metabolismo , COVID-19/genética , Humanos , Edição de RNA/genética , SARS-CoV-2/genética , Transcriptoma/genética
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