RESUMO
Schistosoma mansoni is a heteroxenous parasite, meaning that during its life cycle needs the participation of obligatory intermediate and definitive hosts. The larval development occurs in aquatic molluscs belonging to the Biomphalaria genus, leading to the formation of cercariae, which emerge to infect the final vertebrate host. For this reason, studies for control of the diseases caused by digenetic trematodes often focus on combating the snail hosts. Thus, the objective of this study was to evaluate the susceptibility of Biomphalaria tenagophila embryos to the fungus Pochonia chlamydosporia (isolate Pc-10). The entire experiment was conducted in duplicate, with five replicates for each repetition (five egg masses/replicate), utilizing a total of 100 egg masses, with 20-30 eggs/egg mass. At the end of 15 days, the egg masses were evaluated under a stereomicroscope to analyze the hatching of B. tenagophila embryos in both experimental groups. After days of interaction, the exposure to the fungal hyphae bodies significantly impaired the viability of the B. tenagophila eggs, inhibiting the embryogenesis process by 83.7% in relation to the control group. Transmission and scanning electron microscopic images revealed relevant structural alterations in the egg masses exposed to the hyphae action of the fungus, interfering in the development and hatching of the young snails under analysis. These results indicate the susceptibility of B. tenagophila embryos to the fungus P. chlamydosporia (isolate Pc-10) and suggest the potential of Pc-10 to be used in the control of intermediate host, for its ovicidal capacity and for being an ecologically viable option, but in vivo experiments become necessary.
Assuntos
Ascomicetos , Biomphalaria , Animais , Biomphalaria/parasitologia , Interações Hospedeiro-Parasita , Hypocreales , Schistosoma mansoni , CaramujosRESUMO
This review aims to promote discussion about the situation of fasciolosis in ruminants in Brazil. The disease is still found more frequently in the South and Southeast regions, but reports outside these areas show the spread of the disease, including human cases. Many studies have been published on the diagnosis and control of fasciolosis, but development of field diagnosis methods and drugs that control all stages of the parasite is still a challenge. Studies should be carried out of new distribution areas and alternatives for control in Brazil, which depends on understanding the complex interactions between of the environment, ecosystems and hosts of this trematode.
Esta revisão tem como objetivo levantar uma discussão sobre a situação da fasciolose em ruminantes no Brasil. A enfermidade ainda se encontra mais frequentemente nas regiões Sul e Sudeste do país, entretanto relatos fora dessas regiões evidenciam a expansão da enfermidade, inclusive casos em humanos. Muitos estudos têm sido publicados sobre o diagnóstico e controle da fasciolose, entretanto, o diagnóstico a campo e fármacos que combatam todas as fases evolutivas do parasito ainda são desafios. Novos estudos devem ser realizados sobre novas áreas de distribuição e alternativas de controle em condições brasileiras, o que depende da compreensão das complexas interações entre meio ambiente, ecossistemas e hospedeiros desse trematódeo.
RESUMO
The risk of bovine cysticercosis is mutable over time, since population habits, local infrastructure, and bovine herd size change through the years. Hence, the aim of this study was to update the risk map of bovine cysticercosis occurrence in Espírito Santo State, Brazil, by choosing the following risk factors: inadequate sewer service; bovine population per municipality; risk of flooding; and land use and occupation, examined with a mathematical model using the GIS program ArcGis 10.5. The risk map of occurrence demonstrated that the state is composed of 12.8, 24.5, 36.2 and 26.5% of areas of very high, high, medium, and low risk, respectively. The northern area stood out for areas of very high risk, with the cities of Linhares and São Mateus accounting for 99% of the area with this classification. Of the state's 78 municipalities, 15 had a portion of their territory with very high risk, 11 had over 50% of the territory with high risk and all except Irupi (100% of low risk) had some percentage of medium risk. These findings demonstrate the areas where actions to prevent bovine cysticercosis should be preferentially conducted.
O risco de cisticercose bovina é mutável ao longo do tempo, uma vez que os hábitos populacionais, a infraestrutura local e o tamanho do rebanho bovino mudam ao longo dos anos. Assim, o objetivo deste estudo foi atualizar o mapa de risco da ocorrência da cisticercose bovina no Estado do Espírito Santo, Brasil, escolhendo os seguintes fatores de risco: saneamento sanitário inadequado; população bovina por município; risco de inundação; e uso e ocupação do solo, examinados com um modelo matemático usando o programa SIG ArcGis 10.5. O mapa de risco de ocorrência demonstrou que o estado é composto por 12,8, 24,5, 36,2 e 26,5% de áreas de risco muito alto, alto, médio e baixo, respectivamente. A zona norte destacou-se como área de risco muito alto, com os municípios de Linhares e São Mateus respondendo por 99% da área com essa classificação. Dos 78 municípios do estado, 15 tinham uma parcela de seu território com risco muito alto, 11 tinham mais de 50% do território com risco alto e todos exceto Irupi (100% de risco baixo) tinham algum percentual de risco médio. Esses achados demonstram as áreas onde as ações de prevenção da cisticercose bovina devem ser preferencialmente realizadas.
RESUMO
This work reports an outbreak of eurytrematosis in cattle in the municipality of Ibitirama, southern Espírito Santo State, Brazil. Six cattle were necropsied from August to December 2019, with finding of Eurytrema coelomaticum in the pancreas. A survey of epidemiological data was carried out on the farms along with coproparasitological examination of cattle from the same herd. Parasites were found in all necropsied animals, with different degrees of parasitism, ranging from mild to massive infection (6 - 2000 specimens). Macroscopic analyses of the pancreas revealed changes in 83.33% (5/6) of the cases, and by microscopy, pancreatic fibrosis ranging from Grade I to Grade III was observed. Inspection of the grazing areas confirmed the presence of two intermediate hosts, a terrestrial snail of the Bradybaena genus, with larval forms of the trematode in histological findings, and a grasshopper of the Conocephalus genus. Although none of the cattle showed clinical signs in the coproparasitological examination, 73.80% (31/42) tested positive for E. coelomaticum eggs. This is the first record of an outbreak of eurytrematosis in cattle in Espírito Santo State, indicating the importance of carrying out diagnosis based on epidemiology and necroscopic and parasitological examinations in animals in the region so that appropriate control measures can be adopted.
Este trabalho objetivou relatar um caso de euritrematose em bovinos no município de Ibitirama, Sul do Estado do Espírito Santo, Brasil. Foram necropsiados seis bovinos de agosto a dezembro de 2019, que apresentaram Eurytrema coelomaticum no pâncreas. Foi realizado levantamento de dados epidemiológicos nas propriedades e exames coproparasitológico em bovinos do mesmo plantel. Em todos os animais necropsiados foram encontrados parasitos, com diferentes graus de parasitismo, variando de infeção branda a maciça (6 - 2000 exemplares). Análises macroscópicas dos pâncreas revelaram alterações em 83,33% (5/6) dos casos e, na microscopia, observou-se fibrose pancreática variando de Grau I a Grau III. A inspeção das áreas de pastejo constatou a presença dos dois hospedeiros intermediários, moluscos terrestres do gênero Bradybaena com formas larvares do trematoda em achados histológicos e gafanhotos do gênero Conocephalus. Nenhum dos bovinos apresentou sinais clínicos, no entanto, no exame coproparasitológico, 73,80% (31/42) testaram positivo para ovos de E. coelomaticum. Este é o primeiro registro de surto de euritrematose em bovinos no estado do Espírito Santo, mostrando a importância da realização do diagnostico a partir da epidemiologia e de exames necroscópicos e parasitológicos em animais da região para que sejam adotadas medidas adequadas de controle.
RESUMO
The gastropod Pseudosuccinea columella participates in the dissemination of Fasciola hepatica in the environment, acting as the main intermediate host of this parasite in Brazil. The present study sought to elucidate the possible pathogenic effects of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora on P. columella, by evaluating the influence of infection on alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the concentrations of total protein, uric acid, and urea in the snail's hemolymph. For this, the snails were exposed to EPNs for 24 and 48 h, and for each exposure time, 20 snails were dissected after 7, 14 and 21 days for hemolymph collection. The primary findings suggest a significant proteolysis alongside elevated levels of uric acid and urea in P. columella infected individuals. These findings reveal that H. bacteriophora HP88 infection induced serious changes in the snail's metabolism, triggering important deleterious effects.
Assuntos
Rabditídios , Animais , Ácido Úrico , Caramujos/parasitologia , UreiaRESUMO
Many studies about fasciolosis control have been carried out, whether acting on the adult parasite or in Pseudosuccinea columella, compromising the development of the larval stages. The present study aimed to evaluate, under laboratory conditions, the susceptibility of P. columella to Heterorhabditis bacteriophora HP88, during for 24 and 48 hours of exposure. The snails were evaluated for 21 days for accumulated mortality; number of eggs laid; hatchability rate; biochemical changes; and histopathological analysis. We found that exposure induced a reduction in glucose and glycogen levels, characterizing a negative energy balance, due to the depletion of energy reserves as a result of the direct competition established by the nematode/endosymbiont bacteria complex in such substrates. A mortality rate of 48.25% and 65.52% was observed in the group exposed for 24 h and 48 h, respectively, along with significant impairment of reproductive biology in both exposed groups in relation to the respective controls. The results presented here show that P. columella is susceptible to the nematode H. bacteriophora, with the potential to be used as an alternative bioagent in the control of this mollusk, especially in areas considered endemic for fascioliasis, in line with the position expressed by the World Health Organization Health.
Assuntos
Fasciolíase , Rabditídios , Animais , Controle Biológico de Vetores/métodos , Caramujos/parasitologia , Fasciolíase/veterináriaRESUMO
Dysphania ambrosioides (L.) Mosyakin & Clemants, known as santa maria herb, is a medicinal plant used on pest control and to treat parasitic diseases. Due to its potential for pest control, the aim of this work was to determine the chemical composition of D. ambrosioides essential oil and evaluate its molluscicidal effect on Biomphalaria tenagophila, which is an intermediate host for the Schistosoma mansoni trematode, that is responsible for causing human schistosomiasis. The essential oil extracted from its leaves showed 87% of (Z)-ascaridole and 100% of mortality against B. tenagophila at 10 ppm after 24 h of experiment. LC50 and LC90 values were, respectively, 2.40 (1.90-2.99) ppm and 8.75 (6.39-14.12) ppm. D. ambrosioides essential oil proved to be a promising natural molluscicide against schistosomiasis when compared with commercial products.
Assuntos
Biomphalaria , Chenopodium ambrosioides , Moluscocidas , Óleos Voláteis , Esquistossomose , Animais , Biomphalaria/parasitologia , Humanos , Moluscocidas/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Schistosoma mansoniRESUMO
Commercial synthetic acaricides have selected resistant populations of Rhipicephalus microplus, and generate residues in the environment or in milk/cattle products. In this study, aqueous extracts (AE) from Melia azedarach (Maz), Allium sativum, Capsicum chinense, Nicotiana tabacum (Nta) and Dysphania ambrosioides were evaluated for the bioactivity against the cattle tick. The treatment using Nta or Maz AE resulted in the lowest egg hatching rate (34.0 ± 11% and 25.0 ± 19%), and in the values of reproduction inhibition ranging from 89.0% to 85.3%. Phytochemical screening associated to RP-HPLC/DAD analysis suggested the presence of alkaloids for Nta and gallic acid derivatives and catechins, for Maz. Such results highlighted that the use of Nta and Maz AE can be a promising source of bioactive compounds for the control of infections caused by the cattle tick.
Assuntos
Acaricidas , Amaranthaceae , Amaryllidaceae , Meliaceae , Rhipicephalus , Solanaceae , Animais , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Acaricidas/farmacologia , Verduras , LarvaRESUMO
The objective of this study was to provide the first report of resistance of Haemonchus contortus to monepantel in sheep in Espírito Santo. The study was conducted in a property with history of monepantel use since 2014 and register of low efficacy in studies conducted over the past few years with fecal egg count reduction test. Lambs born on the property (males and females aged approximately 100 days) were selected and after eggs per gram of feces (EPG) analysis on fecal samples, these were divided into two groups: a group treated with monepantel (2.5 mg / Kg) and a control group without anthelmintic treatment. Seven days later, the animals were euthanized to recover parasites from the gastrointestinal tract. The efficacy of the treatment was 61.35% against H. contortus, thus proving that anthelmintic resistance to monepantel was present.
Assuntos
Anti-Helmínticos , Hemoncose , Haemonchus , Doenças dos Ovinos , Aminoacetonitrila/análogos & derivados , Animais , Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Brasil , Resistência a Medicamentos , Fezes , Feminino , Hemoncose/tratamento farmacológico , Hemoncose/veterinária , Masculino , Contagem de Ovos de Parasitas/veterinária , Ovinos , Doenças dos Ovinos/tratamento farmacológicoRESUMO
An increase in exotic animal imports has been noticed in the last decades worldwide. In this scenario, reptiles represent a quite popular group of interest as exotic pets. However, they can harbor pathogens that under inadequate captivity conditions can lead to disease expression and sometimes death. This study performed coprological analysis to investigate gastrointestinal parasites in a private collection of leopard geckos (E. macularius) relating the results with husbandry practices. Twenty-three geckos of 1 to 5 years, weighing 30-65 g, of both sexes were used. Freshly voided fecal samples were collected and centrifugal fecal float technique was performed using Sheather's sugar solution. 65,21% animals were positive for gastrointestinal parasites. No animal presented any clinical signs but previous loss was reported in the colony. Among affected animals, 93,3% presented oxyurid eggs, 33,33% Strongyloidea eggs and 26,66% presented mite eggs. Four animals were infected with both nematodes. The groups found are commonly reported in captive reptiles and, due to their direct life cycle, reinfection is common in captive animals. The husbandry practices proved to be insufficient and preventive measures such as quarantine were not adopted by the owner, which can compromise not only the reptiles health but also people who deal with them.
Assuntos
Enteropatias Parasitárias , Lagartos , Parasitos , Animais , Fezes/parasitologia , Feminino , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/veterinária , Lagartos/parasitologia , MasculinoRESUMO
Dioctophyme renale is the largest parasitic nematode of animals. It is most often found in the right kidney, but can also occur in the urinary system, ears, free abdominal cavity, mammary gland, thoracic cavity, and more rarely in subcutaneous tissue. The genus Dirofilaria has specific parasitic characteristics according to its location, and may parasitize the respiratory tract or even the skin, varying according to species. This report describes a case of Dioctophyme renale and Dirofilaria sp. in the subcutaneous tissue of a cat in the state of Espírito Santo, Brazil. An adult male mongrel cat showed physical enlargement in the left inguinal region, diagnosed as a subcutaneous nodule. Surgical excision and histopathological evaluation of the nodule were performed, and Dioctophyme renale and Dirofilaria sp. were found inside. Dioctophymosis and heartworm disease are present in Brazil and most other countries, but this is the unprecedented case of the association of Dioctophyme renale and Dirofilaria sp. in the subcutaneous tissue of a cat.
RESUMO
The city of Guarapari, known as "Healthy City", has as its main tourist attraction radioactive sands known as monazite sands. There are many studies aimed at quantifying radiation, but little is known about an influence of radiation levels on the life cycle of parasites with zoonotic potential, since many owners take their animals to beaches facilitating a maintenance of their cycle. The objective of the present work was to evaluate the association of the sand radiation of the beaches of the municipality of Guarapari and the presence of eggs and larvae of zoonotic potential parasites. To determine the influence of the radiation on the parasites, sand and faeces samples were collected from the beaches of Praia do Morro, Areia Preta, Castanheiras, Setiba and Santa Mônica, and how to analyze, use the simple centrifugal-flotation technique. The beaches of the present study were classified as low, medium and high radioactivity according to the dose limits that a human being can receive. The samples of sand and faeces were grouped according to a classification of the beaches where they were collected as samples. After obtaining the result, a Chi-square statistical test was performed at a significance level of 95% to evaluate an association between radiation classes and parasite presence. In general, monazitic sands have an influence on the maintenance of the cycle of zoonotic parasites found in beach sand, their correlation being inversely proportional.
O município de Guarapari, balneário conhecido por "Cidade Saúde", possui como principal atrativo turístico as areias radioativas conhecidas como areias monazíticas. Há muitos estudos direcionados a quantificação da radiação, mas pouco se sabe sobre a influência dos níveis de radiação sobre o ciclo de vida dos parasitos com potencial zoonótico, uma vez que muitos proprietários levam seus animais as praias, facilitando a manutenção desses ciclos. O objetivo deste trabalho foi avaliar a associação da radiação da areia monazítica das praias do município de Guarapari e a presença de ovos e larvas de parasitos de potencial zoonótico. Para determinar a influência da radiação sobre os parasitos, coletou-se amostras da Praia do Morro, Areia Preta, Castanheiras, Setiba e Santa Mônica, a partir de areias e de fezes, para a análise utilizou a técnica de centrifugo-flutuação simples. As praias deste estudo foram classificadas em baixa, média e alta radioatividade de acordo com as doses limites que um ser humano pode receber. As amostras de areia e fezes foram agrupadas de acordo com a classificação das praias onde foram recolhidas as amostras. Após obtenção do resultado, foi feito teste estatístico Qui-quadrado a um nível de significância de 95% para avaliar a associação entre as classes de radiação e a presença dos parasitos. Em geral, as areias monazíticas têm influência na manutenção do ciclo de parasitas zoonóticos encontrados na areia da praia, sendo sua correlação inversamente proporcional.
RESUMO
Sixty-two fecal samples of capybara (Hydrochoerus hydrochaeris) living in eight conservation units (CUs) across the state of Espírito Santo, southeastern Brazil, were assessed for the presence of eggs of liver flukes via sedimentation. Fasciola hepatica eggs were found in 37.1% (23/62) of the samples. Positive samples were found in six CUs (75%), three CUs located in the southern region of the state and three others in the metropolitan region of the capital city of Vitória. Identification of Fasciola hepatica eggs collected from capybara fecal samples were based on morphology, and confirmed using molecular methods. Our results suggest that capybaras may serve as a wild reservoir host for F. hepatica, possibly contributing to the epidemiology and geographic range expansion of this zoonotic parasite across its vast range of distribution in South America.
Assuntos
Fasciola hepatica , Fasciolíase , Doenças dos Roedores , Animais , Brasil , Fasciolíase/diagnóstico , Fasciolíase/epidemiologia , Fasciolíase/veterinária , Fezes , RoedoresRESUMO
Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.
Assuntos
Albendazol/administração & dosagem , Anti-Helmínticos/administração & dosagem , Fasciola hepatica/efeitos dos fármacos , Fasciolíase/veterinária , Doenças dos Ovinos/tratamento farmacológico , Ovinos/parasitologia , Animais , Fasciolíase/tratamento farmacológico , Fasciolíase/parasitologia , Fezes/parasitologia , Feminino , Masculino , Contagem de Ovos de Parasitas , Doenças dos Ovinos/parasitologiaRESUMO
Geographical Information Systems (GIS) is frequently used in the control of animal diseases. In Brazil, the most impacting economical loss in the beef supply chain is bovine cysticercosis. This study focused on assessing the prevalence and geospatial distribution of bovine cysticercosis in 19 Brazilian states. To this, we gathered data from 146,346,244 bovines slaughtered between the years of 2010 and 2015. The observed prevalence was 0.62% (C.I. 0.62-0.63). In total, 30.86% cysticerci were viable, while 69.14% were unviable. Bovine cysticercosis cases had a significant decrease (p<0.05) during this period. The states of Paraná (2.01%; C.I. 2.00-2.02), Santa Catarina (1.96%; C.I. 1.93-2.00), São Paulo (1.77%; C.I. 1.76-1.77), Rio Grande do Sul (1.63%; C.I. 1.60-1.63) and Mato Grosso do Sul (0.80%; C.I. 0.80-0.80) had the highest prevalence values. In some states a significant (p<0.05) decreasing trend was detected in the prevalence. In conclusion, Taenia-saginata-cysticercosis remains endemic in Brazil and interventions are necessary to maintain Brazilian beef competitive in the international food market and improve food safety to population.
Assuntos
Doenças dos Bovinos/epidemiologia , Cisticercose/veterinária , Animais , Brasil/epidemiologia , Bovinos , Cisticercose/epidemiologia , Estudos Retrospectivos , Análise Espacial , Taenia saginataRESUMO
Fasciolosis is caused by Fasciola hepatica that affects the bile ducts and liver parenchyma of ruminants, which can result in economic loss. This study aimed to carry out the validity of the commercial kit ELISA® indirect front of the simple fecal sedimentation test used as the standard. 143 samples were collected blood and feces of cattle from Jerome, south of the Espírito Santo. Serum samples were left at -80 °C and used to perform the ELISA kit IDEXX®. All animals to stool examinations were also positive to the ELISA (22) and negative samples to test stool (121), 52 animals reacted positively against the antibody research. The frequency of fasciolosis was 15.4% in the stool examinations and 51.8% by ELISA. The validity was calculated by sensitivity (100%), specificity (57%), positive predictive value (29%) and negative predictive value (100%), and the correlation between the tests calculated using the kappa index of 0.35. The better sensitivity of the ELISA commercial kit should not be a separately evaluated, since the cost benefit and the technical facility must be considered.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Doenças dos Bovinos/sangue , Doenças dos Bovinos/diagnóstico , Fasciola hepatica/imunologia , Fasciolíase/veterinária , Kit de Reagentes para Diagnóstico , Animais , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Ensaio de Imunoadsorção Enzimática , Fasciolíase/diagnóstico , Fasciolíase/epidemiologia , Fezes/parasitologia , Reprodutibilidade dos TestesRESUMO
This study elucidated for the first time, under laboratory conditions, the susceptibility of Lymnaea columella to infective juveniles of Heterorhabditis baujardi LPP7. Exposure to the nematodes induced an average mortality rate of 66.66% in the population of L. columella, with the highest values attained from the second week after exposure onward. In addition, all the reproductive parameters analyzed (total number of eggs, number of egg masses, number of eggs laid/snail, embryo hatching rate and content of galactogen stored in the albumen gland) changed as a result of the infection. The results indicate the occurrence of the phenomenon of parasitic castration in L. columella infected by H. baujardi LPP7, probably through depletion of energy reserves such as galactogen, necessary to meet the intense metabolic demands of the nematode's larval stages. Finally, histopathological analysis demonstrated an intense process of cell disorganization, characterized by the occurrence of granulomatous inflammatory reactions in tissues of exposed snails, induced by the spoliative action of the bacteria/nematode. The results suggest the use of H. baujardi LPP7 as an alternative for biological control of the population of this intermediate host, and thus of the diseases in whose epidemiological chain it participates, especially fasciolosis, in line with the recommendations of the World Health Organization (WHO).
Assuntos
Fasciolíase/transmissão , Lymnaea/parasitologia , Controle Biológico de Vetores/métodos , Rabditídios/fisiologia , Animais , Fasciolíase/prevenção & controle , Interações Hospedeiro-ParasitaRESUMO
Abstract The objective of this study was to provide the first report of resistance of Haemonchus contortus to monepantel in sheep in Espírito Santo. The study was conducted in a property with history of monepantel use since 2014 and register of low efficacy in studies conducted over the past few years with fecal egg count reduction test. Lambs born on the property (males and females aged approximately 100 days) were selected and after eggs per gram of feces (EPG) analysis on fecal samples, these were divided into two groups: a group treated with monepantel (2.5 mg / Kg) and a control group without anthelmintic treatment. Seven days later, the animals were euthanized to recover parasites from the gastrointestinal tract. The efficacy of the treatment was 61.35% against H. contortus, thus proving that anthelmintic resistance to monepantel was present.
Resumo O objetivo deste estudo foi fornecer o primeiro relato de resistência de Haemonchus contortus ao monepantel em ovinos no Espírito Santo. O estudo foi conduzido em uma propriedade com histórico de uso de monepantel, desde 2014, e registro de baixa eficácia em estudos conduzidos nos últimos anos, utilizando-se testes de redução de contagem de ovos por grama de fezes (OPG). Cordeiros nascidos na propriedade (machos e fêmeas com idade aproximada de 100 dias) foram selecionados e, após contagem de OPG, foram divididos em dois grupos: um grupo tratado com monepantel (2,5 mg / kg) e um grupo controle, sem tratamento com anti-helmíntico. Após 7 dias, os animais foram eutanasiados para a recuperação dos parasitos no trato gastrointestinal. O tratamento apresentou eficácia de 61,35% para H. contortus, comprovando que a resistência anti-helmíntica ao monepantel foi presente.
Assuntos
Animais , Masculino , Feminino , Doenças dos Ovinos/tratamento farmacológico , Hemoncose/tratamento farmacológico , Hemoncose/veterinária , Haemonchus , Anti-Helmínticos/uso terapêutico , Anti-Helmínticos/farmacologia , Contagem de Ovos de Parasitas/veterinária , Brasil , Resistência a Medicamentos , Ovinos , Fezes , Aminoacetonitrila/análogos & derivadosRESUMO
A map of the risk of bovine cysticercosis occurrence was developed for the state of Espírito Santo, Brazil, and it was based in a mathematic model based on following variables: inadequate sewage, bovine population by county, use and occupation of the land and flood risks in GIS environ by means of the ArcGIS/ArcINFO 10.1 program. The work aims to spatially analyze the risk of bovine cysticercosis occurrence in the state of Espírito Santo, by means of risk factors related to cysticercosis and compare with the prevalence obtained from slaughterhouses in the same area. The map of risk showed areas high risk and very high risk located mainly in Ecoporanga, Linhares, counties, where the prevalence from slaughterhouses are low, and in two counties of south macro-region, Presidente Kennedy and Itapemirim, where prevalence from slaughterhouses are higher.
RESUMO
The objective of this study was to determine the prevalence and risk factors for the occurrence of endoparasitosis in dogs and cats in Espírito Santo, Brazil. For the study, 345 dogs and 160 cats were examined. Faecal samples from the animals were collected, and owners were interviewed about their handling of their animals. For the diagnosis of the infections, the Willis-Molley, simple centrifugal flotation and formalin-ether sedimentation techniques were performed. The data found in the tests were tabulated and analysed with a chi-square test (p <0.05), and calculation of odds ratios (OR) with confidence intervals of 95% were then performed to determine the association between the variables and the outcome of stool examinations. The prevalence of parasites was 59% for dogs and 54% for cats. The genus Ancylostoma was the most prevalent (45%). Supplying filtered water, not feeding raw foods, standardisation of a defecation site and cleaning up the faeces reduced the chances of developing intestinal parasites. An increased frequency of deworming was also shown to be a protective factor; specifically animals which received anthelmintic more than once a year had a lower predisposition for intestinal parasites.