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1.
Immunol Rev ; 2024 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-39364646

RESUMO

Advances in antibody engineering are being directed at the development of next generation immunotherapeutics with improved potency. Hexamerisation of IgG is a normal physiological aspect of IgG biology and recently described mutations that facilitate this process have a substantial impact upon monoclonal antibody behavior resulting in the elicitation of dramatically enhanced complement-dependent cytotoxicity, Fc receptor function, and enhanced antigen binding effects, such as targeted receptor agonism or microbe neutralization. Whereas the discovery of IgG hexamerisation enhancing mutations has largely focused on residues with exposure at the surface of the Fc-Fc and CH2-CH3 interfaces, our unique approach is the engineering of the mostly buried residue H429 in the CH3 domain. Selective substitution at position 429 forms the basis of Stellabody technology, where the choice of amino acid results in distinct hexamerisation outcomes. H429F results in monomeric IgG that hexamerises after target binding, so called "on-target" hexamerisation, while the H429Y mutant forms pH-sensitive hexamers in-solution prior to antigen binding. Moreover, Stellabody technologies are broadly applicable across the family of antibody-based biologic therapeutics, including conventional mAbs, bispecific mAbs, and Ig-like biologics such as Fc-fusions, with applications in diverse diseases.

2.
Nat Immunol ; 13(12): 1213-21, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23086448

RESUMO

CD46 is a complement regulator with important roles related to the immune response. CD46 functions as a pathogen receptor and is a potent costimulator for the induction of interferon-γ (IFN-γ)-secreting effector T helper type 1 (T(H)1) cells and their subsequent switch into interleukin 10 (IL-10)-producing regulatory T cells. Here we identified the Notch family member Jagged1 as a physiological ligand for CD46. Furthermore, we found that CD46 regulated the expression of Notch receptors and ligands during T cell activation and that disturbance of the CD46-Notch crosstalk impeded induction of IFN-γ and switching to IL-10. Notably, CD4(+) T cells from CD46-deficient patients and patients with hypomorphic mutations in the gene encoding Jagged1 (Alagille syndrome) failed to mount appropriate T(H)1 responses in vitro and in vivo, which suggested that CD46-Jagged1 crosstalk is responsible for the recurrent infections in subpopulations of these patients.


Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Ativação Linfocitária , Proteína Cofatora de Membrana/metabolismo , Proteínas de Membrana/metabolismo , Células Th1/imunologia , Adulto , Síndrome de Alagille/genética , Síndrome de Alagille/imunologia , Animais , Células Cultivadas , Criança , Pré-Escolar , Humanos , Interferon gama/metabolismo , Interleucina-10/imunologia , Interleucina-10/metabolismo , Proteína Jagged-1 , Camundongos , Camundongos SCID , Camundongos Transgênicos , Interferência de RNA , RNA Interferente Pequeno , Proteínas Serrate-Jagged , Células Th1/metabolismo , alfa Catenina/genética
3.
J Biol Chem ; 296: 100513, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33676896

RESUMO

The C1q and TNF related 4 (C1QTNF4) protein is a structurally unique member of the C1QTNF family, a family of secreted proteins that have structural homology with both complement C1q and the tumor necrosis factor superfamily. C1QTNF4 has been linked to the autoimmune disease systemic lupus erythematosus through genetic studies; however, its role in immunity and inflammation remains poorly defined and a cell surface receptor of C1QTNF4 has yet to be identified. Here we report identification of nucleolin as a cell surface receptor of C1QTNF4 using mass spectrometric analysis. Additionally, we present evidence that the interaction between C1QTNF4 and nucleolin is mediated by the second C1q-like domain of C1QTNF4 and the C terminus of nucleolin. We show that monocytes and B cells are target cells of C1QTNF4 and observe extensive binding to dead cells. Imaging flow cytometry experiments in monocytes show that C1QTNF4 becomes actively internalized upon cell binding. Our results suggest that nucleolin may serve as a docking molecule for C1QTNF4 and act in a context-dependent manner through coreceptors. Taken together, these findings further our understanding of C1QTNF4's function in the healthy immune system and how dysfunction may contribute to the development of systemic lupus erythematosus.


Assuntos
Linfócitos B/imunologia , Citocinas/metabolismo , Imunidade Inata/imunologia , Inflamação/imunologia , Monócitos/imunologia , Fosfoproteínas/metabolismo , Proteínas de Ligação a RNA/metabolismo , Receptores de Superfície Celular/metabolismo , Sequência de Aminoácidos , Linfócitos B/citologia , Linfócitos B/metabolismo , Citocinas/genética , Humanos , Inflamação/metabolismo , Inflamação/patologia , Monócitos/citologia , Monócitos/metabolismo , Fosfoproteínas/genética , Proteínas de Ligação a RNA/genética , Receptores de Superfície Celular/genética , Nucleolina
4.
Int J Mol Sci ; 23(21)2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36362241

RESUMO

Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy. We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE. The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC). Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains. Using neuraminidase-A, we generated sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody. Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles. Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition. When cross-linked on the cell surface, both IgEs triggered RBL-SX38 degranulation. We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.


Assuntos
Imunoglobulina E , Ácido N-Acetilneuramínico , Ratos , Animais , Humanos , Receptores de IgE/metabolismo , Receptores Fc , Cromatografia em Gel , Antígenos de Neoplasias
5.
J Immunol ; 203(7): 1693-1700, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31462504

RESUMO

An allergic reaction is rapidly generated when allergens bind and cross-link IgE bound to its receptor FcεRI on effector cells, resulting in cell degranulation and release of proinflammatory mediators. The extent of effector cell activation is linked to allergen affinity, oligomeric state, valency, and spacing of IgE-binding epitopes on the allergen. Whereas most of these observations come from studies using synthetic allergens, in this study we have used Timothy grass pollen allergen Phl p 7 and birch pollen allergen Bet v 4 to study these effects. Despite the high homology of these polcalcin family allergens, Phl p 7 and Bet v 4 display different binding characteristics toward two human patient-derived polcalcin-specific IgE Abs. We have used native polcalcin dimers and engineered multimeric allergens to test the effects of affinity and oligomeric state on IgE binding and effector cell activation. Our results indicate that polcalcin multimers are required to stimulate high levels of effector cell degranulation when using the humanized RBL-SX38 cell model and that multivalency can overcome the need for high-affinity interactions.


Assuntos
Alérgenos/imunologia , Afinidade de Anticorpos , Antígenos de Plantas/imunologia , Proteínas de Ligação ao Cálcio/imunologia , Degranulação Celular , Imunoglobulina E/imunologia , Modelos Imunológicos , Proteínas de Plantas/imunologia , Alérgenos/genética , Antígenos de Plantas/genética , Proteínas de Ligação ao Cálcio/genética , Epitopos/genética , Epitopos/imunologia , Células HEK293 , Humanos , Proteínas de Plantas/genética , Multimerização Proteica/genética , Multimerização Proteica/imunologia
6.
J Biol Chem ; 292(24): 9975-9987, 2017 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-28438838

RESUMO

Immunoglobulin E and its interactions with receptors FcϵRI and CD23 play a central role in allergic disease. Omalizumab, a clinically approved therapeutic antibody, inhibits the interaction between IgE and FcϵRI, preventing mast cell and basophil activation, and blocks IgE binding to CD23 on B cells and antigen-presenting cells. We solved the crystal structure of the complex between an omalizumab-derived Fab and IgE-Fc, with one Fab bound to each Cϵ3 domain. Free IgE-Fc adopts an acutely bent structure, but in the complex it is only partially bent, with large-scale conformational changes in the Cϵ3 domains that inhibit the interaction with FcϵRI. CD23 binding is inhibited sterically due to overlapping binding sites on each Cϵ3 domain. Studies of omalizumab Fab binding in solution demonstrate the allosteric basis for FcϵRI inhibition and, together with the structure, reveal how omalizumab may accelerate dissociation of receptor-bound IgE from FcϵRI, exploiting the intrinsic flexibility and allosteric potential of IgE.


Assuntos
Antiasmáticos/farmacologia , Imunoglobulina E/metabolismo , Modelos Moleculares , Omalizumab/farmacologia , Receptores de IgE/antagonistas & inibidores , Sítio Alostérico , Substituição de Aminoácidos , Cristalografia por Raios X , Transferência Ressonante de Energia de Fluorescência , Humanos , Imunoglobulina E/química , Imunoglobulina E/genética , Fragmentos Fab das Imunoglobulinas/química , Fragmentos Fab das Imunoglobulinas/genética , Fragmentos Fab das Imunoglobulinas/metabolismo , Fragmentos Fab das Imunoglobulinas/farmacologia , Fragmentos Fc das Imunoglobulinas/química , Fragmentos Fc das Imunoglobulinas/genética , Fragmentos Fc das Imunoglobulinas/metabolismo , Fragmentos Fc das Imunoglobulinas/farmacologia , Omalizumab/química , Omalizumab/genética , Omalizumab/metabolismo , Maleabilidade , Mutação Puntual , Conformação Proteica , Domínios e Motivos de Interação entre Proteínas , Redobramento de Proteína , Receptores de IgE/química , Receptores de IgE/metabolismo , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes de Fusão/farmacologia , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia , Solubilidade , Ressonância de Plasmônio de Superfície
7.
J Allergy Clin Immunol ; 139(4): 1195-1204.e11, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27658758

RESUMO

BACKGROUND: Chronic rhinosinusitis with nasal polyps is associated with local immunoglobulin hyperproduction and the presence of IgE antibodies against Staphylococcus aureus enterotoxins (SAEs). Aspirin-exacerbated respiratory disease is a severe form of chronic rhinosinusitis with nasal polyps in which nearly all patients express anti-SAEs. OBJECTIVES: We aimed to understand antibodies reactive to SAEs and determine whether they recognize SAEs through their complementarity-determining regions (CDRs) or framework regions. METHODS: Labeled staphylococcal enterotoxin (SE) A, SED, and SEE were used to isolate single SAE-specific B cells from the nasal polyps of 3 patients with aspirin-exacerbated respiratory disease by using fluorescence-activated cell sorting. Recombinant antibodies with "matched" heavy and light chains were cloned as IgG1, and those of high affinity for specific SAEs, assayed by means of ELISA and surface plasmon resonance, were recloned as IgE and antigen-binding fragments. IgE activities were tested in basophil degranulation assays. RESULTS: Thirty-seven SAE-specific, IgG- or IgA-expressing B cells were isolated and yielded 6 anti-SAE clones, 2 each for SEA, SED, and SEE. Competition binding assays revealed that the anti-SEE antibodies recognize nonoverlapping epitopes in SEE. Unexpectedly, each anti-SEE mediated SEE-induced basophil degranulation, and IgG1 or antigen-binding fragments of each anti-SEE enhanced degranulation by the other anti-SEE. CONCLUSIONS: SEEs can activate basophils by simultaneously binding as antigens in the conventional manner to CDRs and as superantigens to framework regions of anti-SEE IgE in anti-SEE IgE-FcεRI complexes. Anti-SEE IgG1s can enhance the activity of anti-SEE IgEs as conventional antibodies through CDRs or simultaneously as conventional antibodies and as "superantibodies" through CDRs and framework regions to SEEs in SEE-anti-SEE IgE-FcεRI complexes.


Assuntos
Enterotoxinas/imunologia , Pólipos Nasais/imunologia , Rinite/imunologia , Sinusite/imunologia , Asma Induzida por Aspirina/imunologia , Teste de Degranulação de Basófilos , Basófilos/imunologia , Separação Celular , Doença Crônica , Regiões Determinantes de Complementaridade , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Humanos , Imunoglobulina E/imunologia , Imunoglobulina G/imunologia , Masculino , Pessoa de Meia-Idade , Staphylococcus aureus/imunologia , Superantígenos/imunologia , Ressonância de Plasmônio de Superfície
8.
Angew Chem Int Ed Engl ; 57(52): 17194-17199, 2018 12 21.
Artigo em Inglês | MEDLINE | ID: mdl-30408305

RESUMO

Immunoglobulins are biomolecules involved in defence against foreign substances. Flexibility is key to their functional properties in relation to antigen binding and receptor interactions. We have developed an integrative strategy combining ion mobility mass spectrometry (IM-MS) with molecular modelling to study the conformational dynamics of human IgG antibodies. Predictive models of all four human IgG subclasses were assembled and their dynamics sampled in the transition from extended to collapsed state during IM-MS. Our data imply that this collapse of IgG antibodies is related to their intrinsic structural features, including Fab arm flexibility, collapse towards the Fc region, and the length of their hinge regions. The workflow presented here provides an accurate structural representation in good agreement with the observed collision cross section for these flexible IgG molecules. These results have implications for studying other nonglobular flexible proteins.


Assuntos
Imunoglobulina G/química , Gases/química , Espectrometria de Massas , Modelos Moleculares , Conformação Proteica
9.
Biochim Biophys Acta Proteins Proteom ; 1865(11 Pt A): 1336-1347, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28844738

RESUMO

Immunoglobulin E (IgE) is the antibody that plays a central role in the mechanisms of allergic diseases such as asthma. Interactions with its receptors, FcεRI on mast cells and CD23 on B cells, are mediated by the Fc region, a dimer of the Cε2, Cε3 and Cε4 domains. A sub-fragment lacking the Cε2 domains, Fcε3-4, also binds to both receptors, although receptor binding almost exclusively involves the Cε3 domains. This domain also contains the N-linked glycosylation site conserved in other isotypes. We report here the crystal structures of IgE-Fc and Fcε3-4 at the highest resolutions yet determined, 1.75Šand 2.0Šrespectively, revealing unprecedented detail regarding the carbohydrate and its interactions with protein domains. Analysis of the crystallographic B-factors of these, together with all earlier IgE-Fc and Fcε3-4 structures, shows that the Cε3 domains exhibit the greatest intrinsic flexibility and quaternary structural variation within IgE-Fc. Intriguingly, both well-ordered carbohydrate and disordered polypeptide can be seen within the same Cε3 domain. A simplified method for comparing the quaternary structures of the Cε3 domains in free and receptor-bound IgE-Fc structures is presented, which clearly delineates the FcεRI and CD23 bound states. Importantly, differential scanning fluorimetric analysis of IgE-Fc and Fcε3-4 identifies Cε3 as the domain most susceptible to thermally-induced unfolding, and responsible for the characteristically low melting temperature of IgE.


Assuntos
Imunoglobulina E/química , Fragmentos Fc das Imunoglobulinas/química , Receptores de IgE/química , Motivos de Aminoácidos , Sítios de Ligação , Sequência de Carboidratos , Cristalografia por Raios X , Expressão Gênica , Glicosilação , Humanos , Imunoglobulina E/genética , Imunoglobulina E/imunologia , Fragmentos Fc das Imunoglobulinas/genética , Fragmentos Fc das Imunoglobulinas/imunologia , Modelos Moleculares , Transição de Fase , Ligação Proteica , Domínios e Motivos de Interação entre Proteínas , Multimerização Proteica , Estabilidade Proteica , Estrutura Quaternária de Proteína , Estrutura Secundária de Proteína , Desdobramento de Proteína , Receptores de IgE/genética , Receptores de IgE/imunologia , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Temperatura
10.
Proc Natl Acad Sci U S A ; 109(31): 12686-91, 2012 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-22802656

RESUMO

The role of IgE in allergic disease mechanisms is performed principally through its interactions with two receptors, FcεRI on mast cells and basophils, and CD23 (FcεRII) on B cells. The former mediates allergic hypersensitivity, the latter regulates IgE levels, and both receptors, also expressed on antigen-presenting cells, contribute to allergen uptake and presentation to the immune system. We have solved the crystal structure of the soluble lectin-like "head" domain of CD23 (derCD23) bound to a subfragment of IgE-Fc consisting of the dimer of Cε3 and Cε4 domains (Fcε3-4). One CD23 head binds to each heavy chain at the interface between the two domains, explaining the known 2:1 stoichiometry and suggesting mechanisms for cross-linking membrane-bound trimeric CD23 by IgE, or membrane IgE by soluble trimeric forms of CD23, both of which may contribute to the regulation of IgE synthesis by B cells. The two symmetrically located binding sites are distant from the single FcεRI binding site, which lies at the opposite ends of the Cε3 domains. Structural comparisons with both free IgE-Fc and its FcεRI complex reveal not only that the conformational changes in IgE-Fc required for CD23 binding are incompatible with FcεRI binding, but also that the converse is true. The two binding sites are allosterically linked. We demonstrate experimentally the reciprocal inhibition of CD23 and FcεRI binding in solution and suggest that the mutual exclusion of receptor binding allows IgE to function independently through its two receptors.


Assuntos
Imunoglobulina E/química , Complexos Multiproteicos/química , Receptores de IgE/química , Regulação Alostérica/imunologia , Linfócitos B/química , Linfócitos B/imunologia , Cristalografia por Raios X , Humanos , Imunoglobulina E/imunologia , Complexos Multiproteicos/imunologia , Estrutura Quaternária de Proteína , Estrutura Terciária de Proteína , Receptores de IgE/imunologia , Relação Estrutura-Atividade
11.
J Biol Chem ; 288(30): 21667-77, 2013 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-23775083

RESUMO

Immunoglobulin E (IgE) antibodies play a fundamental role in allergic disease and are a target for therapeutic intervention. IgE functions principally through two receptors, FcεRI and CD23 (FcεRII). Minute amounts of allergen trigger mast cell or basophil degranulation by cross-linking IgE-bound FcεRI, leading to an inflammatory response. The interaction between IgE and CD23 on B-cells regulates IgE synthesis. CD23 is unique among Ig receptors in that it belongs to the C-type (calcium-dependent) lectin-like superfamily. Although the interaction of CD23 with IgE is carbohydrate-independent, calcium has been reported to increase the affinity for IgE, but the structural basis for this activity has previously been unknown. We have determined the crystal structures of the human lectin-like head domain of CD23 in its Ca(2+)-free and Ca(2+)-bound forms, as well as the crystal structure of the Ca(2+)-bound head domain of CD23 in complex with a subfragment of IgE-Fc consisting of the dimer of Cε3 and Cε4 domains (Fcε3-4). Together with site-directed mutagenesis, the crystal structures of four Ca(2+) ligand mutants, isothermal titration calorimetry, surface plasmon resonance, and stopped-flow analysis, we demonstrate that Ca(2+) binds at the principal and evolutionarily conserved binding site in CD23. Ca(2+) binding drives Pro-250, at the base of an IgE-binding loop (loop 4), from the trans to the cis configuration with a concomitant conformational change and ordering of residues in the loop. These Ca(2+)-induced structural changes in CD23 lead to additional interactions with IgE, a more entropically favorable interaction, and a 30-fold increase in affinity of a single head domain of CD23 for IgE. Taken together, these results suggest that binding of Ca(2+) brings an extra degree of modulation to CD23 function.


Assuntos
Linfócitos B/metabolismo , Cálcio/metabolismo , Imunoglobulina E/metabolismo , Receptores de IgE/metabolismo , Sequência de Aminoácidos , Sítios de Ligação/genética , Ligação Competitiva , Calorimetria , Cristalografia por Raios X , Ciclofilina A/metabolismo , Entropia , Humanos , Imunoglobulina E/química , Cinética , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Mutação de Sentido Incorreto , Ligação Proteica , Multimerização Proteica , Estrutura Terciária de Proteína , Receptores de IgE/química , Receptores de IgE/genética , Homologia de Sequência de Aminoácidos , Ressonância de Plasmônio de Superfície
12.
J Immunol ; 188(7): 3199-207, 2012 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-22393152

RESUMO

CD23, the low-affinity receptor for IgE, exists in membrane and soluble forms. Soluble CD23 (sCD23) fragments are released from membrane (m)CD23 by the endogenous metalloprotease a disintegrin and metalloprotease 10. When purified tonsil B cells are incubated with IL-4 and anti-CD40 to induce class switching to IgE in vitro, mCD23 is upregulated, and sCD23 accumulates in the medium prior to IgE synthesis. We have uncoupled the effects of mCD23 cleavage and accumulation of sCD23 on IgE synthesis in this system. We show that small interfering RNA inhibition of CD23 synthesis or inhibition of mCD23 cleavage by an a disintegrin and metalloprotease 10 inhibitor, GI254023X, suppresses IL-4 and anti-CD40-stimulated IgE synthesis. Addition of a recombinant trimeric sCD23 enhances IgE synthesis in this system. This occurs even when endogenous mCD23 is protected from cleavage by GI254023X, indicating that IgE synthesis is positively controlled by sCD23. We show that recombinant trimeric sCD23 binds to cells coexpressing mIgE and mCD21 and caps these proteins on the B cell membrane. Upregulation of IgE by sCD23 occurs after class-switch recombination, and its effects are isotype-specific. These results suggest that mIgE and mCD21 cooperate in the sCD23-mediated positive regulation of IgE synthesis on cells committed to IgE synthesis. Feedback regulation may occur when the concentration of secreted IgE becomes great enough to allow binding to mCD23, thus preventing further release of sCD23. We interpret these results with the aid of a model for the upregulation of IgE by sCD23.


Assuntos
Linfócitos B/imunologia , Regulação da Expressão Gênica/imunologia , Genes de Imunoglobulinas , Imunoglobulina E/biossíntese , Receptores de IgE/imunologia , Proteínas ADAM/antagonistas & inibidores , Proteína ADAM10 , Secretases da Proteína Precursora do Amiloide/antagonistas & inibidores , Linfócitos B/metabolismo , Dipeptídeos/farmacologia , Retroalimentação Fisiológica , Homeostase , Humanos , Ácidos Hidroxâmicos/farmacologia , Switching de Imunoglobulina , Capeamento Imunológico , Proteínas de Membrana/antagonistas & inibidores , Proteínas de Membrana/fisiologia , Inibidores de Proteases/farmacologia , Ligação Proteica , Interferência de RNA , RNA Interferente Pequeno/farmacologia , Receptores de Complemento 3d/imunologia , Receptores de IgE/genética , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/farmacologia , Solubilidade , Regulação para Cima
13.
J Biol Chem ; 287(37): 31457-61, 2012 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-22815482

RESUMO

IgE, the antibody that mediates allergic responses, acts as part of a self-regulating protein network. Its unique effector functions are controlled through interactions of its Fc region with two cellular receptors, FcεRI on mast cells and basophils and CD23 on B cells. IgE cross-linked by allergen triggers mast cell activation via FcεRI, whereas IgE-CD23 interactions control IgE expression levels. We have determined the CD23 binding site on IgE, using a combination of NMR chemical shift mapping and site-directed mutagenesis. We show that the CD23 and FcεRI interaction sites are at opposite ends of the Cε3 domain of IgE, but that receptor binding is mutually inhibitory, mediated by an allosteric mechanism. This prevents CD23-mediated cross-linking of IgE bound to FcεRI on mast cells and resulting antigen-independent anaphylaxis. The mutually inhibitory nature of receptor binding provides a degree of autonomy for the individual activities mediated by IgE-FcεRI and IgE-CD23 interactions.


Assuntos
Basófilos/metabolismo , Imunoglobulina E/metabolismo , Mastócitos/metabolismo , Receptores de IgE/metabolismo , Regulação Alostérica/imunologia , Basófilos/citologia , Basófilos/imunologia , Linhagem Celular , Humanos , Imunoglobulina E/genética , Imunoglobulina E/imunologia , Mastócitos/citologia , Mastócitos/imunologia , Mutagênese Sítio-Dirigida , Ressonância Magnética Nuclear Biomolecular , Mapeamento de Peptídeos/métodos , Ligação Proteica , Estrutura Terciária de Proteína , Receptores de IgE/genética , Receptores de IgE/imunologia
14.
J Allergy Clin Immunol ; 130(3): 663-670.e3, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22583928

RESUMO

BACKGROUND: Serum IgG(4) responses to allergen immunotherapy are well documented as blocking allergen binding to receptor-bound IgE on antigen-presenting cells and effector cells, but the molecular characteristics of treatment-induced IgG(4), particularly in relation to expressed antibody, are poorly defined. OBJECTIVES: We aimed to clone and express recombinant IgG(4) from patients receiving grass pollen immunotherapy using single B cells to obtain matched heavy- and light-chain pairs. METHODS: IgG(4)(+) B cells were enriched from blood samples taken from 5 patients receiving grass pollen immunotherapy. Matched heavy- and light-chain variable-region sequences were amplified from single IgG(4)(+) B cells. Variable regions were cloned and expressed as recombinant IgG(4). Binding analysis of grass pollen-specific IgG(4) was performed by using surface plasmon resonance. Functional assays were used to determine IgE blocking activity. In a separate experiment grass pollen-specific antibodies were depleted from serum samples to determine the proportion of grass pollen-specific IgG(4) within total IgG(4). RESULTS: Depletion of grass pollen-specific antibodies from serum led to a modest reduction in total IgG(4) levels. Matched heavy- and light-chain sequences were cloned from single IgG(4)(+) B cells and expressed as recombinant IgG(4). We identified an IgG(4) that binds with extremely high affinity to the grass pollen allergen Phl p 7. Furthermore, we found that a single specific mAb can block IgE-mediated facilitated allergen presentation, as well as IgE-mediated basophil activation. CONCLUSION: Although increases in IgG(4) levels cannot be wholly accounted for within the allergen-specific fraction, allergen immunotherapy might result in the production of high-affinity allergen-specific blocking IgG(4).


Assuntos
Alérgenos/imunologia , Linfócitos B/imunologia , Proteínas de Ligação ao Cálcio/imunologia , Dessensibilização Imunológica , Imunoglobulina G/sangue , Poaceae/imunologia , Rinite Alérgica Sazonal/terapia , Adulto , Sequência de Aminoácidos , Afinidade de Anticorpos , Especificidade de Anticorpos , Antígenos de Plantas , Humanos , Pessoa de Meia-Idade , Dados de Sequência Molecular , Rinite Alérgica Sazonal/imunologia
15.
Antibodies (Basel) ; 12(4)2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-37987253

RESUMO

We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described.

16.
Mol Immunol ; 159: 28-37, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-37267832

RESUMO

Antibodies of the IgD isotype remain the least well characterized of the mammalian immunoglobulin isotypes. Here we report three-dimensional structures for the Fab region of IgD, based on four different crystal structures, at resolutions of 1.45-2.75 Å. These IgD Fab crystals provide the first high-resolution views of the unique Cδ1 domain. Structural comparisons identify regions of conformational diversity within the Cδ1 domain, as well as among the homologous domains of Cα1, Cγ1 and Cµ1. The IgD Fab structure also possesses a unique conformation of the upper hinge region, which may contribute to the overall disposition of the very long linker sequence between the Fab and Fc regions found in human IgD. Structural similarities observed between IgD and IgG, and differences with IgA and IgM, are consistent with predicted evolutionary relationships for the mammalian antibody isotypes.


Assuntos
Fragmentos Fab das Imunoglobulinas , Isotipos de Imunoglobulinas , Animais , Humanos , Mamíferos
17.
J Biol Chem ; 286(35): 30606-30614, 2011 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-21733840

RESUMO

MxiG is a single-pass membrane protein that oligomerizes within the inner membrane ring of the Shigella flexneri type III secretion system (T3SS). The MxiG N-terminal domain (MxiG-N) is the predominant cytoplasmic structure; however, its role in T3SS assembly and secretion is largely uncharacterized. We have determined the solution structure of MxiG-N residues 6-112 (MxiG-N(6-112)), representing the first published structure of this T3SS domain. The structure shows strong structural homology to forkhead-associated (FHA) domains. Canonically, these cell-signaling modules bind phosphothreonine (Thr(P)) via highly conserved residues. However, the putative phosphate-binding pocket of MxiG-N(6-112) does not align with other FHA domain structures or interact with Thr(P). Furthermore, mutagenesis of potential phosphate-binding residues has no effect on S. flexneri T3SS assembly and function. Therefore, MxiG-N has a novel function for an FHA domain. Positioning of MxiG-N(6-112) within the EM density of the S. flexneri needle complex gives insight into the ambiguous stoichiometry of the T3SS, supporting models with 24 MxiG subunits in the inner membrane ring.


Assuntos
Proteínas de Bactérias/química , Proteínas de Membrana/química , Shigella flexneri/metabolismo , Proteínas de Bactérias/fisiologia , Sítios de Ligação , Clonagem Molecular , Vermelho Congo/farmacologia , Sequência Conservada , Corantes Fluorescentes/farmacologia , Espectroscopia de Ressonância Magnética/métodos , Proteínas de Membrana/fisiologia , Modelos Biológicos , Modelos Moleculares , Conformação Molecular , Mutagênese Sítio-Dirigida , Mutação , Fosfatos/química , Fosfotreonina/química , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Transdução de Sinais
18.
Drug Test Anal ; 14(4): 613-621, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34766468

RESUMO

To date, a specific point-of-care test (POCT) for 3,4-methylenedioxymethamphetamine (MDMA, ecstasy, 'E') in latent fingerprints (LFPs) has not been explored. Other POCTs identify MDMA in sweat by detecting the drug as a cross-reactant rather than target analyte, thus decreasing the test's sensitivity. The study's aim was to design a sensitive POCT for the detection of MDMA in LFPs using surface plasmon resonance (SPR) and lateral flow immunoassay (LFA) technology. A high-affinity antibody binding pair was identified using the former technique, deeming the pair suitable for a LFA. Titrations of fluorescently labelled antibody and antigen concentrations were tested to identify a sharp drop-in signal upon the addition of MDMA to allow a clear distinction between negative and positive outcomes. We trialled the LFA by producing dose response curves with MDMA and a group of drugs that share a similar chemical structure to MDMA. These were generated through spiking the LFA with increasing levels of drug (0-400 pg/10 µl of MDMA; 0-10,000 pg/10 µl of cross-reactant). Fluorescent test signals were measured using a cartridge reader. The cut-off (threshold) 60 pg/10 µl calculated better cartridge performance (1.00 sensitivity, 0.95 specificity and 0.98 accuracy), when compared with 40 pg/10 µl. The biggest cross-reactant was PMMA (250%), followed by MDEA (183%), MBDB (167%), MDA (16%) and methamphetamine (16%). A sensitive LFP screening tool requiring no sample preparation was successfully designed.


Assuntos
3,4-Metilenodioxianfetamina , Metanfetamina , N-Metil-3,4-Metilenodioxianfetamina , Anfetaminas , Cromatografia Gasosa-Espectrometria de Massas , N-Metil-3,4-Metilenodioxianfetamina/análise , Testes Imediatos , Detecção do Abuso de Substâncias/métodos , Ressonância de Plasmônio de Superfície , Tecnologia
19.
Biochemistry ; 50(21): 4608-14, 2011 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-21520934

RESUMO

Allergic reactions are triggered by the interaction between IgE and its high-affinity receptor, FcεRI. Various studies have mapped the interaction surface between IgE and its cellular receptors to the third constant domain of IgE (Cε3). The isolated Cε3 domain has been shown to exist as a molten globule, and the domain retains significant flexibility within the context of the IgE protein. Here we have analyzed the structural basis of the intrinsic flexibility of this domain. We have compared the sequence of the Cε3 domain to the sequences of other members of the C1 subset of the immunoglobulin superfamily and observed that Cε3 has an unusually high electrostatic charge and an unusually low content of hydrophobic residues. Mutations restoring Cε3 to a more canonical sequence were introduced in an attempt to derive a more structured domain, and several mutants display decreased levels of disorder. Engineered domains of Cε3 with a range of structural rigidities could serve as important tools for the elucidation of the role of flexibility of the Cε3 domain in IgE's biological functions.


Assuntos
Imunoglobulina E/química , Dicroísmo Circular , Humanos , Imunoglobulina E/genética , Modelos Moleculares , Mutagênese Sítio-Dirigida , Ressonância Magnética Nuclear Biomolecular , Eletricidade Estática
20.
J Exp Med ; 202(6): 751-60, 2005 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-16172256

RESUMO

The low-affinity immunoglobulin E (IgE) receptor, CD23 (FcepsilonRII), binds both IgE and CD21 and, through these interactions, regulates the synthesis of IgE, the antibody isotype that mediates the allergic response. We have determined the three-dimensional structure of the C-type lectin domain of CD23 in solution by nuclear magnetic resonance spectroscopy. An analysis of concentration-dependent chemical shift perturbations have allowed us to identify the residues engaged in self-association to the trimeric state, whereas ligand-induced changes have defined the binding sites for IgE and CD21. The results further reveal that CD23 can bind both ligands simultaneously. Despite the C-type lectin domain structure, none of the interactions require calcium. We also find that IgE and CD23 can interact to form high molecular mass multimeric complexes. The interactions that we have described provide a solution to the paradox that CD23 is involved in both up- and down-regulation of IgE and provide a structural basis for the development of inhibitors of allergic disease.


Assuntos
Imunoglobulina E/metabolismo , Receptores de Complemento 3d/metabolismo , Receptores de IgE/química , Receptores de IgE/metabolismo , Sítios de Ligação , Cálcio/metabolismo , Regulação para Baixo/imunologia , Humanos , Imunoglobulina E/biossíntese , Lectinas/fisiologia , Ligantes , Espectroscopia de Ressonância Magnética , Ligação Proteica , Estrutura Terciária de Proteína , Ressonância de Plasmônio de Superfície , Regulação para Cima/imunologia
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