[Frequency of Gardnerella vaginalis vaginosis and its association with other pathogens causing genital infections in the female]. / Frecuencia de vaginosis producida por Gardnerella vaginalis y su asociación con otros patógenos causantes de infección genital en la mujer.

In order to determine the presence of unspecific vaginosis and their causes, 700 vaginal smears were obtained from patients assisting to the Clinical Laboratory of the Familiar Medicine Unit No. 28 "Gabriel Mancera" of the Instituto Mexicano del Seguro Social, during 6 months. The patients age was from 18 to 55 years old. To establish the infectious etiology of these pathologies the vaginal smears were observed freshly and stained by Gram's method. Specific test for differentiate Gardnerella vaginalis and Candida albicans were also performed. From 700 vaginal smears, 160 were positive to Candida albicans (22.86%); 150 to Gardnerella vaginalis (21.43%); and 14 to Trichomonas vaginalis (2%). The most frequent association were Candida albicans with Gardnerella vaginalis in 14 women, who 12 had a reduced number of pregnancy, and 7 had only one pregnancy (58.3%). The age groups most affected were between 18 and 35 years old, corresponding to the reproductive stage of the woman. Gardnerella vaginalis predominated in the 30-35 years old group.

Seroepidemiological studies of cutaneous leishmaniasis in the Campeche state of México.

Seroepidemiological studies of cutaneous leishmaniasis were carried out in 169 individuals in a rural area of the Campeche state of México. Fifty showed cutaneous lesions suggestive of leishmaniasis, 70% were parasite positive and 96% skin test positive. An overall 40% positivity to skin test with Montenegro's antigen was found. Most of the affected individuals were males from 11 to 30 years-old. Antibodies were determined by immunofluorescent antibody test (IFA) and by Western blot. Two antigen preparations were used, one from a Leishmania mexicana strain which produced localized cutaneous leishmaniasis (LCL) and the other from a diffuse cutaneous leishmaniasis (DCL). In the general population from the area of study 19% gave positive IFA tests with DCL antigen and 20% with LCL antigen while for the patients 67% gave positive IFA tests with DCL and 71% with LCL. By Western blot analysis most of the patients recognized more antigens in the DCL than in the LCL strain. In the DCL strain 78% of patients recognized a 105 kDa, 34% a 139 kDa, 28% a 117 kDa and 26% a 205 kDa MW antigen. In the LCL strain 40% of patients recognized a 205 kDa and 22% a 175 kDa antigens.