RESUMO
A pair of jeans produced false positive results upon testing for the presence of blood using the Kastle-Meyer (KM) test. Positive reactions were obtained from all unstained areas of the fabric tested. The peroxidase used in the manufacture of some jeans may be the causative agent for the observed false positive reactions; however, it was not possible to confirm this theory.
Assuntos
Manchas de Sangue , Teste em Amostras de Sangue Seco , Reações Falso-Positivas , Têxteis/análise , Vestuário , Humanos , Peroxidase , Sensibilidade e EspecificidadeRESUMO
A report on the Cold Spring Harbor Laboratory meeting 'Yeast Cell Biology', Cold Spring Harbor, USA, 12-17 August 2003.
Assuntos
Biologia/métodos , Células , Genômica/métodos , Saccharomyces cerevisiae/genética , Biologia/tendências , Genômica/tendências , Saccharomyces cerevisiae/citologiaRESUMO
Cyclin-dependent kinase (CDK) activity initiates the eukaryotic cell division cycle by turning on a suite of gene expression in late G1 phase. In metazoans, CDK-dependent phosphorylation of the retinoblastoma tumor suppressor protein (Rb) alleviates repression of E2F and thereby activates G1/S transcription. However, in yeast, an analogous G1 phase target of CDK activity has remained elusive. Here we show that the cell size regulator Whi5 inhibits G1/S transcription and that this inhibition is relieved by CDK-mediated phosphorylation. Deletion of WHI5 bypasses the requirement for upstream activators of the G1/S transcription factors SBF/MBF and thereby accelerates the G1/S transition. Whi5 is recruited to G1/S promoter elements via its interaction with SBF/MBF in vivo and in vitro. In late G1 phase, CDK-dependent phosphorylation dissociates Whi5 from SBF and drives Whi5 out of the nucleus. Elimination of CDK activity at the end of mitosis allows Whi5 to reenter the nucleus to again repress G1/S transcription. These findings harmonize G1/S control in eukaryotes.