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Virology ; 562: 63-73, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34265628

RESUMO

We aimed to describe SARS-CoV-2 strains in Iranians from nine distributed cities infected during two months expanding late 2020 and early 2021 by genotyping known informative single nucleotide in five PCR amplicons. Two variants associated with haplotype H1 (clade G) and nine additional variants associated with other haplotypes were genotyped, respectively, in RNA isolates of 244 and 85 individuals. The variants associated with the H1a (GR) and H1b (GH) haplotypes were most prevalent, indicating a significant change in infection pattern with passage of time. The most important findings were that recombinant genomes and co-infection, respectively, were surmised in 44.7% and 12.9% of the samples extensively genotyped. Partners of many of the recombinations were relatively common strains. Co-existing viruses were among those currently circulating in Iran. In addition to random mutations, co-infection with different existing strains and recombination between their genomes may significantly contribute to the emergence of new SARS-CoV-2 strains.


Assuntos
COVID-19/virologia , Variação Genética , Genoma Viral , Recombinação Genética , SARS-CoV-2/genética , Coinfecção/genética , Evolução Molecular , Técnicas de Genotipagem , Haplótipos , Humanos , Mutação , Filogenia , RNA Viral/genética , SARS-CoV-2/isolamento & purificação
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