In silico analysis of non-structural protein 12 sequences from SARS-COV-2 found in Manaus, Amazonas, Brazil, reveals mutations linked to higher transmissibility.

The disease coronavirus COVID-19 has been the cause of millions of deaths worldwide. Among the proteins of SARS-CoV-2, non-structural protein 12 (NSP12) plays a key role during COVID infection and is part of the RNA-dependent RNA polymerase complex. The monitoring of NSP12 polymorphisms is extremely important for the design of new antiviral drugs and monitoring of viral evolution. This study analyzed the NSP12 mutations detected in circulating SARS-CoV-2 during the years 2020 to 2022 in the population of the city of Manaus, Amazonas, Brazil. The most frequent mutations found were P323L and G671S. Reports in the literature indicate that these mutations are related to transmissibility efficiency, which may have contributed to the extremely high numbers of cases in this location. In addition, two mutations described here (E796D and R914K) are close and have RMSD that is similar to the mutations M794V and N911K, which have been described in the literature as influential on the performance of the NSP12 enzyme. These data demonstrate the need to monitor the emergence of new mutations in NSP12 in order to better understand their consequences for the treatments currently used and in the design of new drugs.

Genetic characterization and description of Leishmania (Leishmania) ellisi sp. nov.: a new human-infecting species from the USA.

In a 2018 report, an unusual case of cutaneous leishmaniasis was described in a 72-year-old female patient residing in Arizona, United States of America (USA). Preliminary analysis of the 18S rDNA and glyceraldehyde-3-phosphate dehydrogenase genes supported the conclusion that the Leishmania strain (strain 218-L139) isolated from this case was a novel species, though a complete taxonomic description was not provided. Identification of Leishmania at the species level is critical for clinical management and epidemiologic investigations so it is important that novel human-infecting species are characterized taxonomically and assigned a unique scientific name compliant with the ICZN code. Therefore, we sought to provide a complete taxonomic description of Leishmania strain 218-L139. Phylogenetic analysis of several nuclear loci and partial maxicircle genome sequences supported its position within the subgenus Leishmania and further clarified the distinctness of this new species. Morphological characterization of cultured promastigotes and amastigotes from the original case material is also provided. Thus, we conclude that Leishmania (Leishmania) ellisi is a new cause of autochthonous cutaneous leishmaniasis in the USA.

Knowledge and perception of milk producers about thermal stress in Brazilian dairy farms.

Heat stress is a challenge for the milk production chain, especially in tropical environments. Thus, the objective was to evaluate the knowledge and the perception of milk producers about heat stress and determine what corrective measures they adopted to minimize losses in the productive performance of dairy cows due to high temperatures. A questionnaire was applied to 112 dairy farmers in the states of Minas Gerais and Goiás, Brazil in 2019 and 2020. The collected data were submitted to descriptive statistics using the G test of independence and multivariate correspondence analysis. Among the surveyed producers, 89% stated that they had already been informed about what heat stress is and how it affects the animals; 94% declared that they had already noticed in their day-to-day activities that heat stress impairs productivity and milk quality, and 92% stated that they have tried to reduce the heat stress to which the animals in their herd are exposed. There was an association among previous knowledge about heat stress and farm size, daily volume of milk produced, number of lactating cows, type of milking and presence of technical assistance. There was also an association among the adoption of measures that minimize the negative effects of heat stress with the size of the property, the number of cows in lactation and with the producer's perception of heat stress in their daily lives. It was concluded that, in important municipalities of the Brazilian dairy chain, most milk producers surveyed have knowledge and perception of the negative effects of heat stress on the productive performance of dairy cows and sought to adopt measures that could alleviate them.

Surgery is associated with better long-term outcomes than pharmacological treatment for obesity: a systematic review and meta-analysis.

Obesity is a highly prevalent disease with numerous complications. Both intensive medical treatment with the use of pharmacological drugs and bariatric surgery are current options. The objective of this meta-analysis was to compare, in the long-term, intensive medical treatment and surgery based on twelve parameters related to weight loss, cardiovascular and endocrine changes. A review of the literature was conducted in accordance with the PRISMA guidelines (PROSPERO: CRD42021265637). The literature screening was done from inception to October 2023 through PubMed, EMBASE and Web of Science databases. We included randomized clinical trials that had separate groups for medical treatment and bariatric surgery as an intervention for obesity. The risk of bias was assessed through RoB2. A meta-analysis was performed with measures of heterogeneity and publication bias. Subgroup analysis for each surgery type was performed. Data is presented as forest-plots. Reviewers independently identified 6719 articles and 6 papers with a total 427 patients were included. All studies were randomized controlled trials, three had a follow up of 5 years and two had a follow up of 10 years. Both groups demonstrated statistical significance for most parameters studied. Surgery was superior for weight loss (- 22.05 kg [- 28.86; - 15.23), total cholesterol (- 0.88 [- 1.59; - 0.17]), triglycerides (- 0.70 [- 0.82; - 0.59]), HDL (0.12 [0.02; 0.23]), systolic pressure (- 4.49 [- 7.65; - 1.33]), diastolic pressure (- 2.28 [- 4.25; - 0.31]), Hb glycated (- 0.97 [- 1.31; - 0.62]), HOMA IR (- 2.94; [- 3.52; - 2.35]) and cardiovascular risk (- 0.08; [- 0.10; - 0.05]). Patient in the surgical treatment group had better long term outcomes when compared to the non-surgical group for most clinical parameters.

Corrigendum: Implementing a provisional overarching intervention for COVID-19 monitoring and control in the Brazil-Colombia-Peru frontier.

[This corrects the article DOI: 10.3389/fpubh.2023.1330347.].

Implementing a provisional overarching intervention for COVID-19 monitoring and control in the Brazil-Colombia-Peru frontier.

Introduction: he challenge was to provide comprehensive health resources to a remote and underserved population living in the Brazil-Colombia-Peru border, amid the most disruptive global crisis of the century. Methods: In August 2021, Fundação Oswaldo Cruz Amazonia (FIOCRUZ Amazônia) and partner collaborators implemented an overarching provisional program for SARS-CoV-2 detection and lineages characterization, training of laboratory personnel and healthcare providers, donation of diagnostic supplies and personal protective equipment, and COVID-19 vaccination. The expedition was conducted at the Port of Tabatinga, a busy terminal with an intense flux of people arriving and departing in boats of all sizes, located in the Amazon River basin. Local government, non-profit organizations, private companies, and other stakeholders supported the intervention. Results: The expedition was accomplished in a convergence point, where migrant workers, traders, army personnel, people living in urban areas, and people from small villages living in riversides and indigenous territories are in close and frequent contact, with widespread cross-border movement. Using a boat as a provisional lab and storage facility, the intervention provided clinical and laboratory monitoring for 891 participants; vaccination for 536 individuals; personal protective equipment for 200 healthcare providers; diagnostic supplies for 1,000 COVID-19 rapid tests; training for 42 community health agents on personal protection, rapid test execution, and pulse oximeter management; and hands-on training for four lab technicians on molecular diagnosis. Discussion: Our experience demonstrates that multilateral initiatives can counterweigh the scarcity of health resources in underserved regions. Moreover, provisional programs can have a long-lasting effect if investments are also provided for local capacity building.

Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil.

Dengue virus serotype 2, genotype Cosmopolitan (DENV-2-GII), is one of the most widespread DENV strains globally. In the USA, DENV-2 epidemics have been dominated by DENV-2 genotype Asian-American (DENV-2-GIII), and the first cases of DENV-2-GII were only described in 2019, in Peru, and in 2021 in Brazil. To gain new information about the circulation of DENV-2-GII in Brazil, we sequenced 237 DENV-2 confirmed cases sampled between March 2021 and March 2023 and revealed that DENV-2-GII is already present in all geographic regions of Brazil. The phylogeographic analysis inferred that DENV-2-GII was introduced at least four times in Brazil, between May 2020 and August 2022, generating multiple clades that spread throughout the country with different success. Despite multiple introductions of DENV-2-GII, analysis of the country-wide laboratory surveillance data showed that the Brazilian dengue epidemic in 2022 was dominated by DENV-1 in most states. We hypothesize that massive circulation of DENV-2-GIII in previous years in Brazil might have created a population immune barrier against symptomatic homotypic reinfections by DENV-2-GII, leading to sustained cryptic circulation in asymptomatic cases and localized outbreaks of this new genotype. In summary, our study stresses the importance of arboviral genomic surveillance to close monitoring and better understanding the potential impact of DENV-2-GII in the coming years.

Relacionamentos afetivo-sexuais de jovens rurais no sertão de Pernambuco: entre modos de subjetivação e resistências cotidianas / Affective-sexual relationships of rural youth in the rural area of Pernambuco: between modes of subjectivation and everyday resistance / Relaciones afectivo-sexuales de la juventud rural en el área rural de Pernambuco: entre modos de subjetivación y resistencia cotidiana

Este artigo tem como objetivo analisar como os jovens rurais vivenciam os valores rurais nos seus relacionamentos afetivo-sexuais no Sertão de Pernambuco. O desenho metodológico foi pautado na pesquisa no cotidiano, por meio da observação participante, e de entrevistas com jovens estudantes de uma escola pública da região. Apesar das mudanças nas áreas rurais, os jovens vivenciam valores camponeses alicerçados no interconhecimento, no valor-família, honra familiar e nas hierarquias intergeracional e de gênero. No cotidiano, criam resistências que objetivam outras possibilidades de existência e de vivências afetivo-sexuais, por meio de deslocamentos, linhas de fugas e recusas parciais desses valores.

This article aims to analyze how rural youth experience rural values in their affective-sexual relationships in the rural area of Pernambuco. The methodological design was based on daily research, through participant observation, and interviews with young students from a public school in the region. Despite changes in rural areas, young people experience peasant values based on inter-knowledge, family value, family honor and intergenerational and gender hierarchies. In everyday life, they create resistance, which aim at other possibilities of existence and affective-sexual experiences, through displacements, lines of escape and partial refusals of these values.

Este artículo tiene como objetivo analizar cómo los jóvenes rurales experimentan los valores rurales en sus relaciones afectivo-sexuales en el área rural de Pernambuco. El diseño metodológico se basó en la investigación diaria, a través de la observación participante, y entrevistas con jóvenes estudiantes de una escuela pública de la región. A pesar de los cambios en las zonas rurales, los jóvenes experimentan valores campesinos basados en el interconocimiento, el valor familiar, el honor familiar y las jerarquías intergeneracionales y de género. En la vida cotidiana crean resistencias, que apuntan a otras posibilidades de existencia y vivencias afectivo-sexuales, a través de desplazamientos, líneas de escape y rechazos parciales de estos valores.

The paradox of mental health versus mental disease in elite athletes: a scoping review

Abstract Aim: To analyze the concepts of mental health and illness as well as the instruments used to measure them in the elite athlete. Methods: The scoping review was conducted using PubMed, SPORTDiscus, PsycINFO, and Scielo. The keywords "Mental Health" AND "Sport" AND "Athlete" were used to find studies in peer-reviewed scientific journals published in English, Spanish, and Portuguese. Studies went through four stages of analysis according to PRISMA guidelines. A total of 179 records were included in this review. Results: Recent studies suggest that health and illness should not be recognized as deviations from the norm, but as a changing construct, varying on a continuum, where, at one extreme, we have health; and, at the other, we have illness even coexisting in an orthogonal way. Between the two extremes, there are degrees of psychological well-being and distress, leading from reduced functioning to effective functioning. This indicates the need for psychological support for both the maintenance and enhancement of mental health. An alarming result was that, although most of the studies investigated mental health, they used primarily instruments focused on illness. This new perspective of health-illness brings the need for the development of new instruments that encompass protective factors and symptom screening. Conclusions: We reinforce the need for a paradigm shift in relation to the mental health of elite athletes understanding it as a changing construct that should be constantly improved and/or maintained. This change led to the need for broader assessment instruments that encompass protective factors and illness symptoms.

Scenario validation for clinical simulation: prenatal nursing consultation for adolescents / Validación de escenario para simulado clínico: consulta de enfermería en el prenatal para adolescente / Validação de cenário para simulação clínica: consulta de enfermagem no pré-natal para adolescente

ABSTRACT Objectives: to validate a scenario for clinical simulation: prenatal nursing consultation for adolescents. Methods: methodological study developed from January to December 2019, in five stages (overview, scenario, scenario design, progression, debriefing and assessment). The validation involved four volunteer students, a teacher as a facilitator and four judges. The judges filled out a Likert scale with four responses. Data was analyzed using Microsoft Excel® software, version 2016. Absolute and relative frequencies and the content validity index were calculated, considering a minimum acceptable value of 1.0. Results: the preparation of the scenario was based on the proposed learning objectives. The scenario was validated with a global content validity index equal to 1.0. Final Considerations: the study achieved the proposed objective. This scenario can contribute to preparing nurses to work in the care of pregnant teenagers, a representative public in Brazil that requires specific care.

RESUMEN Objetivos: validar un escenario para simulado clínico, en enseñanza de enfermería, sobre primera consulta de prenatal a gestante adolescente. Métodos: estudio metodológico desarrollado de enero a diciembre de 2019, en cinco etapas (overview, scenario, scenario design progression, debriefing y assessment). La validación involucró cuatro alumnos voluntarios, una docente como facilitadora y cuatro jueces. Los jueces rellenaron una escala Likert con cuatro respuestas. Datos fueran analizados en el software Microsoft Excel®, versión 2016. Calculadas las frecuencias absolutas y relativas y el índice de validez de contenido, considerando valor mínimo aceptable de 1,0. Resultados: la elaboración del escenario partió de los objetivos de aprendizaje propuestos. El escenario fue validado con índice de validez de contenido global igual a 1,0. Consideraciones Finales: el estudio alcanzó el objetivo propuesto. Este escenario puede contribuir para preparar enfermeros para actuación en la atención a gestantes adolescentes, un público representativo en Brasil y que requiere cuidados específicos.

RESUMO Objetivos: validar um cenário para simulação clínica, no ensino de enfermagem, sobre primeira consulta de pré-natal à gestante adolescente. Métodos: estudo metodológico desenvolvido de janeiro a dezembro de 2019, em cinco etapas (overview, scenario, scenario design progression, debriefing e assessment). A validação envolveu quatro alunos voluntários, uma docente como facilitadora e quatro juízes. Os juízes preencheram uma escala Likert com quatro respostas. Os dados foram analisados no software Microsoft Excel®, versão 2016. Calcularam-se as frequências absolutas e relativas e o índice de validade de conteúdo, considerando valor mínimo aceitável de 1,0. Resultados: a elaboração do cenário partiu dos objetivos de aprendizagem propostos. O cenário foi validado com índice de validade de conteúdo global igual a 1,0. Considerações Finais: o estudo alcançou o objetivo proposto. Este cenário pode contribuir para preparar enfermeiros para atuação na atenção às gestantes adolescentes, um público representativo no Brasil e que requer cuidados específicos.

Comparing dextrose prolotherapy with other substances in knee osteoarthritis pain relief: A systematic review

Abstract The objective of this study is to compare the effectiveness of dextrose-prolotherapy with other substances for pain relief in patients with primary knee osteoarthritis. The literature screening was done in January 2021 through Medline (PubMed), EMBASE, and Database of the National Institute of Health based on the following criteria: randomized clinical trials that subjected patients with primary knee osteoarthritis who underwent treatment with dextrose-prolotherapy and other substances for pain relief. Paired reviewers independently identified 3381 articles and included 8 trials that met the eligibility criteria. According to the findings of this review, participants that underwent dextrose-prolotherapy showed improvements between baseline and posterior assessments and when compared to saline injections, but when compared to other substances, the results were not clear. Although dextrose-prolotherapy is a useful treatment method by itself, it is still not possible to clearly affirm that it is superior or inferior to its counterparts. There is an urgent need for further studies to bring more evidence to the field. HIGHLIGHTS Dextrose injections promote deposition of collagen into injured structures through growth factors and inflammatory cells. Dextrose-prolotherapy is a useful treatment method, but it is not superior or inferior to its counterparts.

MICRObiota on BILIOpancreatic malignant diseases [MICROBILIO]: A systematic review

Abstract Introduction: The increase in the incidence of pancreatic and biliary cancers has attracted the search for methods of early detection of diseases and biomarkers. The authors propose to analyze new findings on the association between microbiota and Pancreatic Ductal Adenocarcinoma (PDAC) or Cholangiocarcinoma (CCA). Methods: This systematic review was carried out according to the items of Preferred Reports for Systematic Reviews and Protocol Meta-Analysis (PRISMA-P). This study was registered by the Prospective Register of Systematic Reviews (PROSPERO), identification code CRD42020192748 before the review was carried out. Articles were selected from the PUBMED, EMBASE, and Cochrane databases. Results: Most studies (86.67%) used 16s rRNA as a sequencing method. The main comorbidities found were diabetes mellitus, systemic arterial hypertension, and dyslipidemia. Many studies were limited by the small number of participants, but the biases were mostly low. There was very little concordance about the composition of the microbiome of different sites, for both case and control groups when compared to other studies' results. Bile sample analysis was the one with a greater agreement between studies, as three out of four studies found Escherichia in cases of CCA. Conclusion: There was great disagreement in the characterization of both the microbiota of cases and control groups. Studies are still scarce, making it difficult to adequately assess the data in this regard. It was not possible to specify any marker or to associate any genus of microbiota bacteria with PDAC or CCA.

Enfermagem e visibilidade na pandemia da covid-19: monitoramento de mídia social / Enfermería y visibilidad en la pandemia del covid-19: monitorización de los medios sociales / Nursing and visibility in covid-19 pandemic: social media monitoring

Objetivo analisar as publicações de usuários do Twitter direcionadas à campanha de valorização da Enfermagem, impulsionadas pelos termos #LuteComoUmaEnfermeira e #EnfermagemEuValorizo. Método pesquisa quantitativa, fundamentada nos métodos digitais. A busca foi realizada utilizando as hashtags #LuteComoUmaEnfermeira e #EnfermagemEuValorizo para extração de tweets. Foram selecionados para análise 2.613 tweets. Resultados 22,5% das publicações localizam-se na região Sudeste do país e 9,3% na região Nordeste. Houve mais de 18 mil interações (curtidas e compartilhamentos), sendo que os autores das postagens atingem um público estimado de cerca 7 milhões de pessoas. O conteúdo das postagens versava principalmente sobre a regulamentação da jornada de trabalho, Equipamentos de Proteção Individual, combate ao COVID-19, defesa da força de trabalho majoritariamente feminina e luta por direitos. Conclusão a realização da campanha virtual teve visibilidade importante no território nacional e apresentou grande visibilidade à profissão em número e alcance na Internet.

Objetivo analizar las publicaciones de los usuarios de Twitter dirigidas a la campaña de valoración de la Enfermería, impulsada por los términos #LuteComoUmaEnfermeira y #EnfermagemEuValorizo. Método investigación cuantitativa, basada en métodos digitales. La búsqueda se llevó a cabo utilizando los hashtags #LuteComoUmaEnfermeira y #EnfermagemEuValorizo para la extracción de tweets. Resultados el 22,5% de las publicaciones se localizan en la región Sudeste del país y el 9,3% en la región Nordeste. Hubo más de 18.000 interacciones (curtidas y compartidas), y los autores de los mensajes llegaron a un público estimado de unos 7 millones de personas. El contenido de los puestos se refería principalmente a la regulación de los horarios de trabajo, el equipo de protección personal, la lucha contra el COVID-19, la defensa de la fuerza de trabajo mayoritariamente femenina y la lucha por los derechos. Conclusión la realización de la campaña virtual tuvo una importante visibilidad en el territorio nacional y presentó una gran visibilidad a la profesión en número y alcance en Internet.

Objective analyze the publications of Twitter users directed to the campaign of valuation of Nursing, driven by the terms #LuteComoUmaEnfermeira and #EnfermagemEuValorizo. Method quantitative research, based on digital methods. The search was realized using the hashtags #LuteComoUmaEnfermeira and #EnfermagemEuValorizo for tweets extraction. 2,613 tweets were selected for analysis. Results 22.5% of publications are located in the Brazilian Southeast region and 9.3% in the Northeast region. There were more than 18,000 interactions (likes and shares), and the authors of the posts reach an estimated audience of about 7 million people). The content of the posts mainly related to the regulation of working hours, Personal Protective Equipment, the fight against COVID-19, the defense of the majority female workforce and the fight for rights. Conclusion the realization of the virtual campaign had important visibility in the national territory and presented great visibility to the profession in number and reach on the Internet.

A judicialização da saúde no Brasil sob as lentes do modelo paradigmático de Strauss e Corbin: uma análise bibliométrica / Health ́s judicialization in Brazil under the lens of the paradigmatic model of Strauss and Corbin: a bibliometric analysis / La judicialización de la salud en Brasil bajo la lente del modelo paradigmático de Strauss y Corbin: un análisis bibliométrico

Objetivo: identificar, nas variáveis propostas pelo modelo paradigmático de Strauss e Corbin, as características da produção científica sobre a judicialização da saúde no contexto dos impactos e desafios gerados para o Sistema Único de Saúde. Métodos: trata-se de um estudo bibliométrico, descritivo, de abordagem quanti-qualitativa. A coleta de dados ocorreu em junho de 2020, resultando num universo de 63 artigos. Para a categorização dos dados adotou-se o modelo paradigmático de Strauss e Corbin. Na análise quantitativa foram consideradas as seguintes variáveis: regiões do Brasil e instituições de onde os estudos foram concebidos; ano de publicação; formação profissional dos autores; periódicos de publicação; descritores utilizados; esfera de governo abordada; e os tipos de pesquisa. No aspecto qualitativo foram analisadas as principais conclusões dos artigos. Resultados: as publicações da região Sudeste foram a maioria. As conclusões dos artigos apontaram que a judicialização como garantia de acesso à saúde pode ser compreendida tanto como uma causa do fenômeno quanto como uma consequência dele. O risco orçamentário para gestão pública e as relações entre o Poder Executivo e Judiciário configuraram os fatores intervenientes. Conclusão: o diálogo institucional constitui um caminho a ser perseguido na tentativa de minimizar possíveis efeitos danosos da judicialização da saúde.

Objective: this study aims to identify in the variables proposed by the paradigmatic model of Strauss and Corbin, the characteristics of the articles that address in their contents, the health ́s judicialization in the context of the impacts and challenges generated for the Unified Health System. Methods: this is a bibliometric, descriptive study, with a quanti-qualitative approach. Data collection took place in June 2020, resulting in a universe of 63 articles. For data categorization, the paradigmatic model of Strauss and Corbin was adopted. In the quantitative analysis, the following variables were considered: regions of Brazil and institutions where the studies were affiliated; year of publication; professional training of authors; publishing periodicals; descriptors used; sphere of government addressed; and types of research. In the qualitative aspect, the main conclusions of the articles were analyzed. Results: publications from the Southeast region were the majority. The conclusions of the articles pointed out that judicialization as a guarantee of access to health care can be understood both as a cause of the phenomenon and because of it. Budgetary risk for public management and the relationship between the Executive and the Judiciary set the intervening factors. Conclusion: institutional dialogue is a path to be pursued to minimize possible harmful effects of health ́sjudicialization.

Objetivo: identificar, en las variables propuestas por el modelo paradigmático de Strauss y Corbin, las características de la producción científica sobre la judicialización de la salud en el contexto de los impactos y desafíos generados para el Sistema Único de Salud. Metodología: esta es una bibliométrica, estudio descriptivo, con enfoque cuanti-cualitativo. La recolección de datos tuvo lugar en junio de 2020, lo que resultó en un universo de 63 artículos. Para la categorización de datos se adoptó el modelo paradigmático de Strauss y Corbin. En el análisis cuantitativo se consideraron las siguientes variables: regiones de Brasil e instituciones donde se diseñaron los estudios; año de publicación; formación profesional de autores; publicación de publicaciones periódicas; descriptores utilizados; esfera de gobierno abordada; y tipos de investigación. En el aspecto cualitativo, se analizaron las principales conclusiones de los artículos. Resultados: las publicaciones de la región Sudeste fueron mayoritarias. Las conclusiones de los artículos señalaron que la judicialización como garantía de acceso a la salud puede entenderse tanto como causa del fenómeno como consecuencia de este. El riesgo presupuestario para la gestión pública y la relación entre el Poder Ejecutivo y el Poder Judicial marcan los factores que intervienen. Conclusión: el diálogo institucional es un camino para seguir en intento por minimizar los posibles efectos nocivos de la judicialización de la salud.

Vigilância socioassistencial no contexto do território: uma análise a partir do Censo Suas / Socio-assistance vigilance in the context of territory: analysis based on Censo Suas

Este artigo apresenta uma reflexão sobre a função da vigilância socio assistencial enquanto diagnóstico do território, com base nos dados do Censo Suas de 2016 e 2017. Para isso, realiza uma pesquisa documental, norteada pelo método do materialismo histórico. Os resultados demonstram uma retração em alguns serviços socioassistenciais dos Centros de Referência de Assistência Social (Cras), em decorrência do avanço da perspectiva neoliberal no desmonte das políticas sociais

This article presents a reflection on the role of social assistance surveillance as a diagnosis of the territory, based on data from the Suas Census of 2016 and 2017. For this purpose, it conducts a documentary research, guided by the method of historical materialism. The results demonstrate a retraction in some social assistance services of the Cras, the advancement of the neoliberal perspective in the dismantling of social policies

Brazilian transgender children and adolescents: Attributes associated with quality of life / Crianças e adolescentes transgêneros brasileiros: atributos associados à qualidade de vida / Niños y adolescentes transgénero brasileños: atributos asociados a la calidad de vida

Objective: to describe attributes associated with the Quality of Life of Brazilian transgender children and adolescents according to their own perception. Method: descriptive study conducted with 32 participants between eight and 18 years old, who were either interviewed or participated in focus groups. The statements were transcribed, grouped with the aid of the Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires software, version 0.7 alpha 2 and described according to the definition of Quality of Life by the World Health Organization concerning to the mental, physical, and social dimensions. Results: it was possible to identify the family nucleus as the main social support for transgender children and adolescents. However, the experience of prejudice and discrimination were negative attributes associated with Quality of Life. Conclusion: the statements indicate that lives of transgender children and adolescents are impacted by social, physical, and mental factors due to the stigma and discrimination experienced. It is expected to contribute to the formulation of public policies related to transgender children and adolescents and expand the discussion on the citizens' duties and rights in relation to transsexuality.

Objetivo: descrever atributos associados à Qualidade de Vida de crianças e adolescentes transgêneros brasileiros segundo sua própria percepção. Método: estudo descritivo, realizado com 32 participantes entre oito e 18 anos, entrevistados ou que participaram de grupos focais. Os depoimentos foram transcritos, agrupados com auxílio do software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires, versão 0.7 alpha 2 e descritos segundo a definição da Qualidade de Vida pela Organização Mundial da Saúde no que concerne às dimensões mental, física e social. Resultados: foi possível identificar o núcleo familiar como o principal meio de suporte social das crianças e adolescentes transgêneros. Entretanto, a vivência de preconceito e discriminação foram atributos negativos associados à Qualidade de Vida. Conclusão: Los depoimentos indicam que a vida das crianças e adolescentes transgêneros é impactada por fatores sociais, físicos e mentais em virtude do estigma e discriminação vivenciados. Espera-se com esse estudo contribuir para a formulação de políticas públicas relacionadas às crianças e adolescentes transgêneros e ampliar a discussão sobre deveres e direitos dos cidadãos frente à transexualidade.

Objetivo: describir los atributos asociados a la Calidad de Vida de niños y adolescentes transgénero brasileños, de acuerdo con su percepción. Método: estudio descriptivo, realizado con 32 participantes entre 8 y 18 años, entrevistados o que participaron en grupos focales. Los testimonios se transcribieron y clasificaron con el soporte del software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires, versão 0.7 alpha 2 y fueron descriptos de acuerdo con la definición de Calidad de Vida de la Organización Mundial de la Salud en lo concerniente a las dimensiones mental, física y social. Resultados: fue posible identificar al núcleo familiar como el principal medio de soporte social para niños y adolescentes transgénero. Sin embargo, haber vivido experiencias de prejuicio y discriminación fueron atributos negativos asociados con la Calidad de Vida. Conclusión: los testimonios indican que la vida de los niños y adolescentes transgénero se ve afectada por factores sociales, físicos y mentales debido al estigma y la discriminación que experimentan. Se espera que este estudio contribuya a la formulación de políticas públicas relacionadas con los niños y adolescentes transgénero y amplíe la discusión sobre los deberes y derechos de los ciudadanos en relación a la transexualidad.

Benefícios psicológicos do slackline: relação entre estado de fluxo, tempo de prática e qualidade de vida / Psychological benefits of slackline: relationship between flow state, time of practice and quality of life / Beneficios psicológicos del slackline: relación entre estado de flujo, tiempo de práctica y calidad de vida

Esse estudo teve o objetivo de avaliar e mensurar o nível de estado de fluxo de praticantes de slackline e relacioná-lo com a qualidade de vida dos indivíduos. Foram avaliados 30 sujeitos com idade média de 22 (±3) anos. Os instrumentos utilizados: Ficha de identificação; Flow State Scale-2 (FSS-2), Dispositional Flow Scale-2 (DFS-20) e World Health Organization Quality of Life-Bref (WHOQoL-BREF). Para verificar a correlação entre os instrumentos utilizou-se o coeficiente de Spearman, no qual foi possível observar elevados níveis de estado de fluxo e qualidade de vida geral. Foi encontrada correlação significativa entre o fator psicológico da qualidade de vida com a disposição para atingir o fluxo juntamente com o tempo de prática e duração da sessão de treino. Concluímos que a prática do slackline possibilita o desenvolvimento de aspectos psicológicos, como o estado de fluxo e com isso possibilita a melhora na qualidade de vida de seus praticantes.

This study aimed to assess the level of Flow State of slackline practitioners and to relate it to the their quality of life. We evaluated 30 subjects with mean age of 22 (± 3) years. The instruments used: Identification sheet; Flow State Scale-2 (FSS-2), Dispositional Flow Scale-2 (DFS-20) and World Health Organization Quality of Life-Bref (WHOQoL-BREF). To verify the correlation between the instruments the Spearman coefficient was used, in which it was possible to observe high levels of Flow State and general quality of life. A significant correlation was found between the psychological quality of life factor and the disposition to reach the Flow along with the practice time and duration of the training session. We conclude that the practice of slackline allows the development of psychological aspects, such as the Flow State and with that allows the improvement in the quality of life of its practitioners.

Este estudio tuvo el objetivo de evaluar el nivel de Estado de Flujo de practicantes de slackline y relacionarlo con la calidad de vida de los individuos. Se evaluaron 30 sujetos con edad media de 22 (± 3) años. Los instrumentos utilizados: Ficha de identificación; Flow State Scale-2 (FSS-2), Dispositional Flow Scale-2 (DFS-20) e World Health Organization Quality of Life-Bref (WHOQoL-BREF). Para verificar la correlación entre los instrumentos se utilizó el coeficiente de Spearman, en el cual fue posible observar elevados niveles de Estado de Flujo y calidad de vida general. Se encontró correlación significativa entre el factor psicológico de la calidad de vida con la disposición para alcanzar el Flujo junto con el tiempo de práctica. Concluimos que la práctica del slackline posibilita el desarrollo de aspectos psicológicos, como el Estado de Flujo y con ello posibilita la mejora en la calidad de vida de sus practicantes.

Bibliometria das publicações da revista 'Saúde em Debate': uma fotografia da saúde mental / Bibliometrics of the publications of the journal 'Saúde em Debate': a photograph of Mental Health

RESUMO O estudo objetivou realizar uma análise bibliométrica dos artigos publicados na revista 'Saúde em Debate', sobre a temática da saúde mental, no contexto da Reforma Psiquiátrica Brasileira, no período de 2012 a 2019. Foi realizada uma busca no acervo on-line da revista em outubro de 2019. Inicialmente, os artigos foram exportados para o software Zotero Standalone 5.0®, sendo selecionados para análise textual e bibliométrica 66 textos. Em seguida, esses textos foram transferidos para o LibreOffice Writer e, posteriormente, analisados no IRaMuTeQ (Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires). Observou-se que a maior parte das publicações ocorreu no estado de São Paulo (18,2%), nos anos de 2014 (24,2%) e 2017 (25,8%), e utilizavam abordagem qualitativa (40,9%). Na classificação hierárquica descendente, o conteúdo analisado foi dividido em sete classes que deram origem à quatro categorias temáticas. A vastidão desse conteúdo textual exposta na análise de similitude e nas categorias temáticas emergidas denota a complexidade do campo de cuidados em saúde mental e aponta para a necessidade de engajamento da sociedade, dos profissionais de saúde e de pesquisadores, para superação de desafios que se ancoram em condutas ainda controversas diante dos avanços alcançados.

ABSTRACT The study aimed to carry out a bibliometric analysis of the articles published in the journal 'Saúde em Debate', on the theme of mental health, in the context of the Brazilian Psychiatric Reform, in the period from 2012 to 2019. The search was carried out in the journal's online collection in October 2019. Initially, the articles were exported to the Zotero Standalone 5.0® software, and 66 texts were selected for textual and bibliometric analysis. Those texts were then transferred to LibreOffice Writer and later analyzed on IRaMuTeQ (Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires). It was observed that most publications occurred in the state of São Paulo (18.2%), in 2014 (24.2%) and 2017 (25.8%) and used a qualitative approach (40.9%). In the descending hierarchical classification, the analyzed content was divided into seven classes that gave rise to four thematic categories. The vast textual content exposed in the similitude analysis and in the thematic categories that emerged denote the complexity of the field of mental health care and point to the need for the engagement of society, health professionals and researchers to overcome challenges anchored in conduct that are still controversial, in view of the advances achieved.

Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil

A new coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterisation of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate was obtained from an asymptomatic carrier returning from Madrid, Spain. Nucleotide sequence analysis showed a total of nine mutations in comparison with the original human case in Wuhan, China, and support this case as belonging to the recently proposed lineage A.2. Phylogeographic analysis further confirmed the likely European origin of this case. To our knowledge, this is the first SARS-CoV-2 genome obtained from the North Brazilian Region. We believe that the information generated in this study may contribute to the ongoing efforts toward the SARS-CoV-2 emergence.

DISABKIDS® in Brazil: advances and future perspectives for the production of scientific knowledge / DISABKIDS® no Brasil: avanços e perspectivas futuras na produção do conhecimento científico / DISABKIDS® en Brasil: avances y perspectivas futuras en la producción del conocimiento científico

Objective: to map the Brazilian scientific production related to the stages of the methodological process for the use of DISABKIDS® instruments and/or forms adapted to Brazil. Method: scoping review, with searches conducted on10 electronic databases, plus Google Scholar and contacts with researchers, without restriction of period or language. Results: the mapping identified 90 scientific studies involving 46 instruments. Of these, 11 (23.9%) included the elaboration and/or cultural adaptation of the DISABKIDS® instruments to measure the Quality of Life of children or adolescents with chronic conditions and 35 (76.1%) used the Generic Measures and/or Specific Modules for the semantic validation of other instruments. Conclusion: this scoping review allowed a comprehensive evaluation of the use of the DISABKIDS® instrument and forms, in relation to the validation of the instrument adapted to Brazil, presenting a positive advance in the scenario with the development of academic/scientific projects in the country, incorporating the method recommended by the literature for the elaboration, cultural adaptation and validation of instruments and for the systematized and standardized recording of the perception and understanding of the target population about the measure of interest, using DISABKIDS® forms adapted for this purpose.

Objetivo: mapear a produção científica brasileira que versa sobre as fases do processo metodológico para a utilização dos instrumentos e/ou formulários DISABKIDS® adaptados para o Brasil. Método: scoping review realizada através da busca em 10 bases de dados eletrônicas, no Google Scholar e por meio de consulta à pesquisadores, sem restrição de período ou idioma. Resultados: o mapeamento identificou 90 estudos científicos que envolveram 46 instrumentos, dos quais 11 (23,9%) foram realizados os processos de elaboração e/ou adaptação cultural dos instrumentos DISABKIDS® para mensuração da Qualidade de Vida de crianças ou adolescentes com condições crônicas e em 35 (76,1%) foram utilizados o Formulários de Impressões Gerais e/ou Folhas Específicas para realizar a validação semântica de outros instrumentos. Conclusão: constatou-se que essa scoping review permitiu avaliação abrangente do uso do instrumento e dos documentos DISABKIDS®. Em relação à validação do instrumento adaptado ao Brasil apresenta avanço positivo do cenário junto ao desenvolvimento de projetos acadêmicos/científicos no país, a partir da incorporação ao método preconizado pela literatura para elaboração, adaptação cultural e validação de instrumentos, do registro sistematizado e padronizado da percepção, bem como do entendimento da população alvo sobre a medida de interesse, ao lançar mão de formulários DISABKIDS® adaptados para esse fim.

Objetivo: mapear la producción científica que se ocupa de las etapas del proceso metodológico para el uso de los instrumentos y/o formularios adaptados DISABKIDS® a Brasil. Método: scoping review, búsqueda realizada en 10 bases de datos electrónicas, en el Google Scholar y por intermedio de consulta a investigadores, sin restricción de período o idioma. Resultados: el mapeo identificó 90 estudios científicos que involucraron 46 instrumentos, de los cuales 11 (23,9%) la elaboración y/o adaptación cultural de los instrumentos DISABKIDS® se realizaron para medir la Calidad de Vida de niños o adolescentes con afecciones crónicas y en 35 (76,1%) utilizaron los Formularios de Impresiones Generales y/o Hojas Específicas para realizar la validación semántica de otros instrumentos. Conclusión: se encontró que esta revisión de alcance permitió una evaluación exhaustiva del uso del instrumento y los documentos DISABKIDS®. En cuanto a la validación del instrumento adaptado a Brasil presenta un avance positivo del escenario en conjunto al desarrollo de proyectos académicos/científicos en el país, desde la incorporación al método recomendado por la literatura para la elaboración, adaptación cultural y validación de instrumentos, desde el registro sistematizado y estandarizado de la percepción, así como la comprensión de la población objetivo sobre la medida de interés mediante el uso de formularios DISABKIDS® adaptados con esta finalidad.