Genome-wide association study of stage III/IV grade C periodontitis (former aggressive periodontitis) in a Spanish population.

AIM: To identify loci associated with stages III/IV, grade C periodontitis (PIII/IV-C) through a genome-wide association study (GWAS). MATERIALS AND METHODS: 441 Caucasian Spanish PIII/IV-C cases from the SEPA Network of Research Clinics and 1141 controls from the Banco Nacional de ADN were genotyped with "Axiom Spain Biobank Array," which contains 757836 markers, including rare and low-frequency Spanish variants. The analysis of the individual association and subsequently the gene-level analysis with Sequence Kernel Association Test (SKAT) were carried out adjusting for age, sex and PC1 covariates. Pathway Analysis was additionally performed with Ingenuity Pathway Analysis (IPA) software on the top associated genes. RESULTS: In the individual analyses, no genome-wide significant signals were detected. However, 8 SNPs of 8 loci reached suggestive evidence of association with PIII/IV-C, including FAT3 rs35709256, CSNK1G2 rs4807188, MYH13 rs2074872, CNTN2 rs116611488, ANTXR1 rs4854545, 8p23.2 rs78672540, ANGPT1 rs13439823 and PLEC rs11993287 (p < 5 × 10-6 ). SKAT analysis identified other interesting signals at CNTN2, FBXO44, AP1M2, RSPO4, KRI1, BPIFB1 and INMT, although their probability does not exceed the multiple-test correction. IPA indicated significant enrichment of pathways related to cAMP, IL-2, CD28, VDR/RXR and PI3K/Akt.  CONCLUSIONS: GWAS found no SNPs significantly associated with PIII/IV-C.

European Association for Osseointegration Delphi study on the trends in Implant Dentistry in Europe for the year 2030.

OBJECTIVES: To assess the potential trends for the year 2030 in dental implant dentistry in Europe using the Delphi methodology. MATERIAL AND METHODS: A steering committee and a management team of experts in implant dentistry were created and validated a questionnaire including 60 questions, divided in eight topics. The survey was conducted in two rounds using an anonymous questionnaire, which provided the participants in the second round with the results of the first. Each question had three possible answers, and the results were expressed as percentages. RESULTS: A total of 138 experts were invited to participate in the survey. From all the invited experts, 52 answered in both the first and second rounds. Three different consensus categories were established based on the percentage of agreement: no consensus (<65%); moderate consensus (65%-85%); and high consensus (≥86%). Within the topic categories, a consensus was reached (mainly moderate consensus) for the majority of questions discussed among experts during a face to face consensus meeting. However, consensus was not reached for a small number of questions/topics. CONCLUSIONS: About 82% of the questions reached consensus. The consensus points towards a lower number of implants to replace chewing units, with implants surfaces made of bioactive materials with reduced micro-roughness using mainly customized abutments with polished surfaces and an internal implant-abutment connection (85%). CBCT-3D technologies will be the main tool for pre-surgical implant placement diagnosis together with direct digital restorative workflows. There will be an increase in the incidence of peri-implantitis, although there will be more efficient interventions its treatment and prevention.

Introduction of a prediction model to assigning periodontal prognosis based on survival time.

AIMS: To develop a prediction model for tooth loss due to periodontal disease (TLPD) in patients following periodontal maintenance (PM), and assess its performance using a multicentre approach. MATERIAL AND METHODS: A multilevel analysis of eleven predictors of TLPD in 500 patients following PM was carried out to calculate the probability of TLPD. This algorithm was applied to three different TLPD samples (369 teeth) gathered retrospectively by nine periodontist, associating several intervals of probability with the corresponding survival time, based on significant differences in the mean survival time. The reproducibility of these associations was assessed in each sample (One-way ANOVA and pairwise comparison with Bonferroni corrections). RESULTS: The model presented high specificity and moderate sensitivity, with optimal calibration and discrimination measurements. Seven intervals of probability were associated with seven survival time and these associations contained close to 80% of the cases: the probability predicted the survival time at this percentage. The model performed well in the three samples, as the mean survival time of each association were significantly different within each sample, while no significant differences between the samples were found in pairwise comparisons of means. CONCLUSIONS: This model might be useful for predicting survival time in different TLPD samples.

Consensus document on the use of antibiotic prophylaxis in dental surgery and procedures.

The goal of antibiotic prophylaxis in Odontology is to prevent the onset of infections through the entranceway provided by the therapeutic action, therefore it is indicated providing there is a considerable risk of infection, either because of the characteristics of the operation itself or the patient s local or general condition. Nonetheless, clinical trials with antibiotics in dental pathologies have had scant regard for the required methodological criteria and, in addition, are not sufficiently numerous. This text presents the results of an expert conference comprising the Presidents of the most representative Scientific Societies in Spain who have analyzed the existing literature and have drawn on their valuable professional experience. It describes the technical circumstances, analyzes the biological and pharmacological foundations and their application to the most representative medical situations. It is concluded that antibiotic prophylaxis in Odontology has certain well-founded, precise indications and offers the international scientific community a practical protocol for action.

Relationship Between Long-Term Marginal Bone Loss and Bone Quality, Implant Width, and Surface.

PURPOSE: Short- or long-term implant survival and success are related to peri-implant marginal bone loss (MBL), among other key factors. The purpose of this study was to analyze the role of clinical and implant-related variables in MBL over a long-term follow-up. MATERIALS AND METHODS: A retrospective study of 558 implants in 172 patients was conducted, analyzing the relationship between MBL and clinical, implant-related, and prosthetic design-related variables. MBL was measured on digital radiographs with specific software, using implant threads as reference. RESULTS: Linear mixed analysis revealed the following significant effects: a lower mean MBL for type IV bone (0.047 mm/year, 95% CI [-0.019, 0.119]) than for type III bone (0.086 mm/year, 95% CI [0.038, 0.138]), type II bone (0.112 mm/year, 95% CI [0.070, 0.167]), or type I bone (0.138 mm/year, 95% CI [0.052, 0.23]); an increased MBL of 0.033 mm/year for each increment of 1 mm in diameter (95% CI [0.002, 0.065]); a lower mean MBL in smooth implants (0.103 mm/year, 95% CI [0.090, 0.117]) vs rough implants (0.122 mm/year, 95% CI [0.102, 0.142]). The mean MBL was > 0 mm/year for all prostheses except for fixed complete dental prostheses. CONCLUSION: Within the limits of a retrospective follow-up study, a lower mean peri-implant MBL was associated with type IV bone, a smaller diameter, a smooth surface, and a fixed complete dental prosthesis.

Multivariate study of factors influencing primary dental implant stability.

OBJECTIVES: The purpose was to determine by multivariate analysis in a large series of dental implants the variables associated with primary endosseous dental implant stability (DIS). MATERIAL AND METHODS: A 10-year retrospective study was conducted of 1084 Brånemark implants placed in 316 patients. Clinical variables (age, gender, smoking habit, and periodontal status), implant diameter, implant length, and Periotest values (PTVs) were analyzed in bivariate and multivariate studies in order to determine their influence on DIS, using a cut-off PTV value of -2. RESULTS: The site of implant insertion showed the strongest association with primary DIS failure among the study variables. Implants in the anterior mandible had a 6.43-fold lower risk of primary DIS risk vs. those at other sites [95% confidence interval (CI) 3.28-12.61], and implants in the maxillary had a 2.70-fold higher risk of primary DIS failure vs. those in the mandible (95% CI 1.82-4). Among other variables, females had a 1.54-fold higher risk of primary DIS failure vs. males (95% CI 1.88-2.22) and implants <15 mm in length had a 1.49-fold higher risk of failure vs. longer implants (95% CI 1.09-2.04). CONCLUSION: According to these findings, primary DIS failure is more likely in females, at sites other than the anterior mandible, and with dental implants shorter than 15 mm, at least when non-threaded titanium implants are used. These data may be of value in the identification of patients at a high risk of primary DIS failure with immediate implant loading.

Early implant failure. Prognostic capacity of Periotest: retrospective study of a large sample.

OBJECTIVES: The objectives of this study were to determine the accuracy of Periotest to monitor primary implant stability at first-stage surgery, to identify by multivariate analysis the variables associated with early implant failure and to compare Periotest with radiographic study in the diagnosis of implant stability at second-stage surgery (during osseointegration period). MATERIAL AND METHODS: A 10-year retrospective study was conducted on 1084 Brånemark implants placed in 316 patients. Clinical variables, implant diameter and length, Periotest values (PTVs) and radiological variables were analyzed in bivariate and multivariate studies in order to determine their influence on early implant failure. RESULTS: After examination of the sensitivity and specificity values obtained for different PTV cutoff points, a cutoff PTV of -2 was selected (84% sensitivity and 39% specificity). In the bivariate analysis, early failure was significantly related to smoking habits, implant location, bone type, implant features and PTVs (-2 and >or=-2). In the final multiple logistic model, only age (odds ratio (OR)=4.53; 95% confidence interval (CI), 1.34-15.27), smoking habits (OR=2.5; 95% CI, 1.3-4.79), bone type (OR=1.93; 95% CI, 1.01-3.7) and PTV at first surgery (OR=3.01; 95% CI, 1.5-6.02) were independently related to early failure. CONCLUSIONS: The Periotest (with -2 cutoff) at first surgery offers high sensitivity in the prognosis of early implant loss and shows a greater capacity to evaluate stability during the osseointegration period compared with radiographic study.