Chlorophyllin protects HEp-2 cells from nuclear fragmentation induced by poliovirus.

AIMS: Chlorophyllin (CHLN) is a synthetic derivative of chlorophyll that possesses antimutagenic activity against several environmental contaminants. In the present study, CHLN was assayed for its capacity to prevent nuclear fragmentation (NF) in HEp-2 cells infected with poliovirus. METHODS AND RESULTS: CHLN was assayed at concentrations of 0.5 and 2.5 microg ml(-1), and NF was monitored using the comet assay and acridine orange staining. We demonstrated that CHLN reduced the percentage of NF in poliovirus-infected HEp-2 cells, when cells were treated with drug before infection or exposed continuously to drug. However, the highest degree of protection was achieved when the virus was exposed to CHLN before infection followed by protocol where infected cultures were continuously exposed to the drug after infection. CONCLUSIONS: It is suggested that CHLN primarily binds to the virus which inhibits cell penetration, thereby maintaining nuclear integrity. SIGNIFICANCE AND IMPACT OF THE STUDY: Considering that CHLN has several beneficial properties and no significant toxic effects in humans and animals, it would be an ideal candidate drug to test for antiviral activity.

Siringoma eruptivo / Eruptive syringoma

Los siringomas son tumores anexiales benignos con diferenciación principalmente ductal. Se presentan con mayor frecuencia en la cara, especialmente en párpados, pero pueden aparecer en cualquier zona del cuerpo. Son más frecuentes en el sexo femenino y se inician por lo general en la pubertad. Pueden aparecer en forma de pápulas pequeñas y firmes, habitualmente múltiples, y existen variadas presentaciones clínicas. Presentamos dos casos clínicos que corresponden a dos mujeres con el diagnóstico de siringomas eruptivos.

Syringomas are benign adnexal tumors with mainly ductal differentiation, more frequently seen in the face, especially in the eyelids, yet may appear in any part of the body. In general, they start a puberty, being more frequent in women. They may present as multiple, small and firm papules, and there are various clinical types. We present two clinical cases corresponding to two women with eruptive syringoma.