Laser phototherapy at high energy densities do not stimulate pre-osteoblast growth and differentiation.

OBJECTIVE: The aim of this study is to evaluate the effects of red and infrared lasers at high energy densities on pre-osteoblast MC3T3 proliferation and differentiation. BACKGROUND DATA: The acceleration of bone regeneration by low intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. MATERIALS AND METHODS: Cells were irradiated with red (660 nm) and infrared (780 nm) lasers (90 and 150 J/cm2, 40 mW). The control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, 96 h) and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72, 96 h; 7, 14 days). RESULTS: None of the irradiation groups had an enhancement in cell growth (p<0.05). The production of ALP was not influenced by irradiation at any period of time (p>0.05). CONCLUSIONS: The low intensity laser stimulated neither cell growth nor the production of alkaline phosphatase.

Incisão vertical para remoção de osso autógeno da sínfise mentual: relato de dois casos clínicos / Vertical incision to autogenous bone removal from the mental symphysis: report of two clinical cases

O presente artigo apresenta o relato de dois casos clínicos onde através de uma técnica alternativa de incisão vertical foram removidos tecidos ósseos da região de sínfise mentual para aumento de volume ósseo em outra região para posterior reabilitação com implante osseo integrado. No primeiro relato em um paciente de 40 anos foi removido osso autógeno em bloco da sínfise mentual com a técnica da incisão vertical para aumento vertical na região da pré-maxila associado com biomaterial e posteriormente instalados dois implantes cilíndricos na região dos 12 e 22. O segundo relato, em paciente com 72 anos, removeu osso particulado da região de sínfise mentual e procedeu-se a enxertia do seio maxilar através da técnica da janela lateral e ambas as áreas utilizaram a técnica da incisão vertical.Constatou-se em ambos os casos minimização de edemas e sintomatologia dolorosa pós-operatórias e nenhum paciente relatou parestesia na região doadora, algo comum em casos com a remoção pela técnica tradicional.

This paper presents a report of two cases where using an alternative technique of vertical incision were removed bone tissue of the symphysis chin region to increase bone volume in another region for later rehabilitation with osseointegrated implant. The first report on a 40 year old patient was removed autogenous bone in the chin symphysis block with the vertical incision technique for vertical increase in pre maxilla associated with biomaterial and then installed two cylindrical implants in the region of 12 and 22. The second account in patients with 72 years removed particulate bone symphysis chin region and decided to graft the maxillarysinus through the lateral window technique and both areas have used the technique of vertical incision. It was found in both cases minimizing swelling and postoperative painful symptoms and no patients reported numbness in the donor area, something common in cases with removing the traditional technique.